8 research outputs found
Desensitization of P2U receptor in neuronal cell line. Different control by the agonists ATP and UTP, as demonstrated by single-cell Ca2+ responses
No full textTwo Types of Neurons in the Rat Cerebellar Nuclei as Distinguished by Membrane Potentials and Intracellular Fillings
No full textA Comparative Voltage and Current-Clamp Analysis of Feedback and Feedforward Synaptic Transmission in the Striatal Microcircuit In Vitro
No full textTwo−photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo
No full textMultiphoton imaging (MPI) is widely used for recording activity simultaneously from many neurons in superficial cortical layers in vivo. We combined regenerative amplification multiphoton microscopy (RAMM) with genetically encoded calcium indicators to extend MPI of neuronal population activity into layer 5 (L5) of adult mouse somatosensory cortex. We found that this approach could be used to record and quantify spontaneous and sensory−evoked activity in populations of L5 neuronal somata located as much as 800 μm below the pia. In addition, we found that RAMM could be used to simultaneously image activity from large (80) populations of apical dendrites and follow these dendrites down to their somata of origi
