Influencia del sexo, la edad y el índice de masa corporal en la anatomía del colon sano. Variaciones anatómicas del colon sano

Objective: This study analyzes the influence of sex, age and body mass index on the anatomy of the colon as determined by three-dimensional reconstructions from computed tomography scans. Methods: 130 patients were included, each of whom underwent a three-dimensional reconstruction of the colon from abdominal CT scans without obvious signs of pathology. Information corresponding to sex, age and body mass index was obtained from each patient, and morphometric data obtained from measurements taken at the pubic symphysis, superior and inferior mesenteric artery, hepatic angle, splenic angle, ileocecal junction and sigmoid-descending colon junction were considered. Results: Morphological analysis showed an important anatomical variability of the reconstructed colon. Statistical analysis showed significant differences in the measurements obtained when grouping subjects by sex, age (under 65 years vs over 65 years) and body mass index (normopese, overweight and obese). Conclusions: Colon anatomical variations can be studied with three-dimensional reconstructions from CT scans, indicating that sex, age and body mass index have a significant influence on such variability.Objetivo: Este estudio analiza la influencia del sexo, la edad y el índice de masa corporal en la anatomía del colon, determinada por reconstrucciones tridimensionales, a partir de tomografías computarizadas. Metodología: Se incluyeron 130 pacientes, a cada uno de los cuales se les realizó una reconstrucción tridimensional del colon, a partir de tomografías computarizadas abdominales sin signos evidentes de patología. De cada paciente se obtuvo información correspondiente a las variables sexo, edad e índice de masa corporal, y se consideraron los datos morfométricos obtenidos de las mediciones realizadas en la sínfisis púbica, arteria mesentérica superior e inferior, ángulo hepático, ángulo esplénico, unión ileocecal y unión del colon sigmoideo-descendente. Resultados: El análisis morfológico mostró una importante variabilidad anatómica del colon reconstruido. El análisis estadístico mostró diferencias significativas en las medidas obtenidas al agrupar a los sujetos por sexo, edad (menores de 65 años vs mayores de 65 años) e índice de masa corporal (normopeso, sobrepeso y obesidad). Conclusiones: Las variaciones anatómicas del colon pueden estudiarse con reconstrucciones tridimensionales a partir de tomografías computarizadas, lo que indica que el sexo, la edad y el índice de masa corporal tienen una influencia significativa en dicha variabilidad

Variation in Susceptibility to Downy Mildew Infection in Spanish Minority Vine Varieties

Downy mildew is one of the most destructive diseases affecting grapevines (Vitis vinifera L.). Caused by the oomycete Plasmopara viticola (Berk. and Curt.) Berl. and de Toni, it can appear anywhere where vines are cultivated. It is habitually controlled by the application of phytosanitary agents (copper-based or systemic) at different stages of the vine growth cycle. This, however, is costly, can lead to reduced yields, has a considerable environmental impact, and its overuse close to harvest can cause fermentation problems. All grapevines are susceptible to this disease, although the degree of susceptibility differs between varieties. Market demands and European legislation on viticulture and the use of phytosanitary agents (art. 14 of Directive 128/2009/EC) now make it important to know the sensitivity of all available varieties, including minority varieties. Such knowledge allows for a more appropriate use of phytosanitary agents, fosters the commercial use of these varieties and thus increases the offer of wines associated with different terroirs, and helps identify material for use in crop improvement programmes via crossing or genetic transformation, etc. Over 2020–2021, the susceptibility to P. viticola of 63 minority vine varieties from different regions of Spain was examined in the laboratory using the leaf disc technique. Some 87% of these varieties were highly susceptible and 11% moderately susceptible; just 2% showed low susceptibility. The least susceptible of all was the variety Morate (Madrid, IMIDRA). Those showing intermediate susceptibility included the varieties Sanguina (Castilla la Mancha, IVICAM), Planta Mula (Comunidad Valenciana, ITVE), Rayada Melonera (Madrid, IMIDRA), Zamarrica (Galicia, EVEGA), Cariñena Roja (Cataluña, INCAVI), Mandrègue (Aragón, DGA) and Bastardo Blanco (Extremadura, CICYTEX). The highly susceptible varieties could be differentiated into three subgroups depending on sporulation severity and density

Variation in Susceptibility to Downy Mildew Infection in Spanish Minority Vine Varieties

Downy mildew is one of the most destructive diseases affecting grapevines (Vitis vinifera L.). Caused by the oomycete Plasmopara viticola (Berk. and Curt.) Berl. and de Toni, it can appear anywhere where vines are cultivated. It is habitually controlled by the application of phytosanitary agents (copper-based or systemic) at different stages of the vine growth cycle. This, however, is costly, can lead to reduced yields, has a considerable environmental impact, and its overuse close to harvest can cause fermentation problems. All grapevines are susceptible to this disease, although the degree of susceptibility differs between varieties. Market demands and European legislation on viticulture and the use of phytosanitary agents (art. 14 of Directive 128/2009/EC) now make it important to know the sensitivity of all available varieties, including minority varieties. Such knowledge allows for a more appropriate use of phytosanitary agents, fosters the commercial use of these varieties and thus increases the offer of wines associated with different terroirs, and helps identify material for use in crop improvement programmes via crossing or genetic transformation, etc. Over 2020–2021, the susceptibility to P. viticola of 63 minority vine varieties from different regions of Spain was examined in the laboratory using the leaf disc technique. Some 87% of these varieties were highly susceptible and 11% moderately susceptible; just 2% showed low susceptibility. The least susceptible of all was the variety Morate (Madrid, IMIDRA). Those showing intermediate susceptibility included the varieties Sanguina (Castilla la Mancha, IVICAM), Planta Mula (Comunidad Valenciana, ITVE), Rayada Melonera (Madrid, IMIDRA), Zamarrica (Galicia, EVEGA), Cariñena Roja (Cataluña, INCAVI), Mandrègue (Aragón, DGA) and Bastardo Blanco (Extremadura, CICYTEX). The highly susceptible varieties could be differentiated into three subgroups depending on sporulation severity and density.This work, performed by the VIOR (Viticultura, Olivo y Rosa) group of the Misión Biológica de Galicia (CSIC), forms part of the project “Valorización de variedades minoritarias de vid por su potencial para la diversificación vitivinícola. Resiliencia a enfermedades fúngicas influenciadas por el cambio climático” (MINORVIN) (RTI 2018-101085-RC32), funded by MCIN/AEI/, 10.13039/501100011033 and the European Regional Development Fund.info:eu-repo/semantics/publishedVersio

Structural characterization of PaaX, the main repressor of the phenylacetate degradation pathway in Escherichia coli W: A novel fold of transcription regulator proteins

15 p.-8 fig.-2 tab.PaaX is a transcriptional repressor of the phenylacetic acid (PAA) catabolic pathway, a central route for bacterial aerobic degradation of aromatic compounds. Induction of the route is achieved through the release of PaaX from its promoter sequences by the first compound of the pathway, phenylacetyl-coenzyme A (PA-CoA). We report the crystal structure of PaaX from Escherichia coli W. PaaX displays a novel type of fold for transcription regulators, showing a dimeric conformation where the monomers present a three-domain structure: an N-terminal winged helix-turn-helix domain, a dimerization domain similar to the Cas2 protein and a C-terminal domain without structural homologs. The domains are separated by a crevice amenable to harbour a PA-CoA molecule. The biophysical characterization of the protein in solution confirmed several hints predicted from the structure, i.e. its dimeric conformation, a modest importance of cysteines and a high dependence of solubility and thermostability on ionic strength. At a moderately acidic pH, the protein formed a stable folding intermediate with remaining α-helical structure, a disrupted tertiary structure and exposed hydrophobic patches. Our results provide valuable information to understand the stability and mechanism of PaaX and pave the way for further analysis of other regulators with similar structural configurations.This research was funded by the following sources: Grants PID2019-105126RB-I00, PID2022-139209OB-C21 (MCIN/AEI/10.13039/501100011033/and ERDF A way of making Europe), TED2021-129747B-C22 (AEI/10.13039/501100011033/NextGenerationEU/PRTR) and CIBER-Consorcio Centro de Investigación Biomédica en Red (CIBERES, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación, Spain) to JMS; grants PID2020-115331GB-100 funded by MCIN/AEI/10.13039/501100011033 and CRSII5_198737/1 (Swiss National Science Foundation) to JAH; grant PID2021-128751NB-I00 (MICINN/AEI/FEDER/UE) to IU, and grant RYC2021-030916-I by the Spanish Agencia Estatal de Investigación to RM. VMH-R was supported by a FPU PhD fellowship from Spanish Ministerio de Educación y Ciencia.Peer reviewe

Association of Candidate Gene Polymorphisms With Chronic Kidney Disease: Results of a Case-Control Analysis in the Nefrona Cohort

Chronic kidney disease (CKD) is a major risk factor for end-stage renal disease, cardiovascular disease and premature death. Despite classical clinical risk factors for CKD and some genetic risk factors have been identified, the residual risk observed in prediction models is still high. Therefore, new risk factors need to be identified in order to better predict the risk of CKD in the population. Here, we analyzed the genetic association of 79 SNPs of proteins associated with mineral metabolism disturbances with CKD in a cohort that includes 2, 445 CKD cases and 559 controls. Genotyping was performed with matrix assisted laser desorption ionizationtime of flight mass spectrometry. We used logistic regression models considering different genetic inheritance models to assess the association of the SNPs with the prevalence of CKD, adjusting for known risk factors. Eight SNPs (rs1126616, rs35068180, rs2238135, rs1800247, rs385564, rs4236, rs2248359, and rs1564858) were associated with CKD even after adjusting by sex, age and race. A model containing five of these SNPs (rs1126616, rs35068180, rs1800247, rs4236, and rs2248359), diabetes and hypertension showed better performance than models considering only clinical risk factors, significantly increasing the area under the curve of the model without polymorphisms. Furthermore, one of the SNPs (the rs2248359) showed an interaction with hypertension, being the risk genotype affecting only hypertensive patients. We conclude that 5 SNPs related to proteins implicated in mineral metabolism disturbances (Osteopontin, osteocalcin, matrix gla protein, matrix metalloprotease 3 and 24 hydroxylase) are associated to an increased risk of suffering CKD

Anestesia con halotano en conejos: a propósito de su utilización en 21 intervenciones abdominales

El conejo es una de las especies más utilizadas en el campo de la experimentación biomédica en todas sus ramas, y concretamente en la investigación quirúrgica. Las técnicas anestésicas descritas para esta especie, aunque son muy diversas, no presen tan un alto margen de seguridad, lo que hace considerar al conejo como la especie animal de experimentación más difícil de anestesiar sin un alto riesgo El conejo plantea una serie de problemas que complican las diversas técnicas anestésicas.The rabbit is one of the most used species in the field of biomedical experimentation in all its branches, and specifically in surgical research. The anesthetic techniques described for this species, although very diverse, do not present a high safety margin, which means that the rabbit is considered the most difficult experimental animal species to anesthetize without a high risk. The rabbit poses a series of problems that complicate the various anesthetic techniques.peerReviewe

Técnica quirúrgica y alteraciones morfológicas de la vasectomía en équidos

Se realiza la vasectomía en el conducto deferente del lado izquierdo en un asno de unos 20 años de edad. Tras describir la técnica quirúrgica se estudian también los cambios macro y microscópicos que aparecen 22 meses después tanto en el testículo como en el epidídimo.We describe the surgical technique of a vasectomy which was performed in the left deferent duct of a 20 years old donkey. The macroscopic and microscopic changes in testes and epididymis were analysed twenty-two months later operation.peerReviewe

Colonic Electrical Stimulation for Chronic Constipation: A Perspective Review

Chronic constipation affects around 20% of the population and there is no efficient solution. This perspective review explores the potential of colonic electric stimulation (CES) using neural implants and methods of bioelectronic medicine as a therapeutic way to treat chronic constipation. The review covers the neurophysiology of colonic peristaltic function, the pathophysiology of chronic constipation, the technical aspects of CES, including stimulation parameters, electrode placement, and neuromodulation target selection, as well as a comprehensive analysis of various animal models highlighting their advantages and limitations in elucidating the mechanistic insights and translational relevance for CES. Finally, the main challenges and trends in CES are discussed