Gajenje karaša (Carassius carassius) u ribnjacima

Uzgoj juvenilnih jedinki karaša (C. carassius) analiziran je u pet ribnjačkih objekata veličine 100 m2. Karaš je gajen u monokulturi u dva, dok je sa linjakom gajen u bikulturi u tri ribnjačka jezera. Stopa preživljavanja karaša u monokulturi iznosila je 21.15±6.86 %, a u bikulturi 47.07±16.86%. Kod linjaka je zabeležena veca stopa preživljavanja (69.33±16.76) i brži rast u odnosu na karaša. Iako je prema dobijenim rezultatima teško proceniti razlike između uzgoja u monokulturi i bikulturi, može se zaključiti da linjak nije značajan kompetitor karašu

Testing cryopreserved European eel sperm for hybridization (A. japonica × A. anguilla)

[EN] The objective of this study was to assess impact of cryopreserved European eel sperm and Japanese eel native sperm on early fertilization, hatch, survival, and malformation rates of larvae, as well as develop molecular techniques to distinguish different eel species. Eggs from Japanese eel females (Anguilla japonica) were artificially fertilized with sperm of Japanese eel males and cryopreserved sperm from European eel (A. anguilla, extender was modified Tanaka solution and methanol as cryoprotectant). There were no statistical differences (p¿>¿0.05) among the measured parameters such as fertilization, hatch and survival after 10 days post-hatch rates due to large individual differences. The malformation rate of larvae compared to the hatching rate was higher in cryopreserved groups than in the control indicating that the methodology needs further refinement. Genetic analyses (PCR-RFLP, PCR-HRM) proved a clear result in the detection of paternal contribution in hybridization between the Japanese and the European eel and applied PCR-HRM method is a quick and cost effective tool to identify illegally imported A. anguilla at the glass eel stage, which can be transported from Europe to Asia.The research was supported by The Ministry of Education, Culture, Sports, Science and Technology (MEXT)/Japan Society for the Promotion of Science (JSPS) Kakenhi Grant No.15K07562 and Tokyo University of Agriculture Strategic Research Program (TUA-SRP), Mohamed bin Zayed Species Conservation Fund (grant number 12252178), GINOP-2.3.2-15-2016-00054 project of the National Research, Development and Innovation Office of Hungary and EFOP-3.6.3-VEKOP-16-2017-00008 project. The project is co-financed by the European Union, the European Social Fund and KMR_12-1-2012-0435.Müller, T.; Matsubara, H.; Kubara, Y.; Horváth, Á.; Kolics, B.; Taller, J.; Stéger, V.... (2018). Testing cryopreserved European eel sperm for hybridization (A. japonica × A. anguilla). Theriogenology. 113:153-158. https://doi.org/10.1016/j.theriogenology.2018.02.021S15315811

Sperm cryopreservation in sturgeon with a special focus on A. sturio: chap. 35

International audienceCryopreservation is a technique of low-temperature conservation of genetic resources for an unlimited period of time The cryopreservation of sturgeon sperm has been problematic for many years, however, during the last decade a reliable methodology has been described that helped to maximize the fertilizing capacity of frozen sturgeon semen. This techique involves the use of methanol as cryoprotectant instead of the more conventional dimethyl-sulfoxide. The method has been successfully applied to Acipenser sturio and currently a cryopreserved gene bank is being developed for this species

Sites of simian foamy virus persistence in naturally infected African green monkeys: Latent provirus is ubiquitous, whereas viral replication is restricted to the oral mucosa

Foamy viruses (FV), retroviruses of the genus Spumavirus, are able to infect a wide variety of animal species and replicate in nearly all types of cultured cells. To identify the cells targeted by FV in the natural host and define the sites of viral replication, multiple organs of four African green monkeys naturally infected with simian Ri type 3 were investigated for the presence of FV proviral DNA and viral transcripts. All organs contained significant amounts of FV proviral DNA. In addition to proviruses containing the complete transactivator gene taf, proviral genomes carrying a specific 295-bp deletion in the taf gene were detected in all monkeys. As in the case of human foamy virus the deletion leads to the formation of the bet gene that is regarded to be instrumental in the regulation of viral persistence. FV RNA was detected by RT-PCR and in situ hybridization only in the oral mucosa of one monkey. No other samples contained detectable levels of viral transcripts. Histopathological changes were not observed in any of the tissue samples analyzed. Our results show that the natural history of FV is characterized by latent infection in all organs of the host and by minimal levels of harmless viral replication in the oral mucosa. The broad host cell range in vivo further encourages the development of FV-derived vectors for therapeutic gene delivery.Foamy viruses (FV), retroviruses of the genus Spumevirus, are able to infect a wide variety of animal species and replicate in nearly all types of cultured cells. To identify the cells targeted by FV in the natural host and define the sites of viral replication, multiple organs of four African green monkeys naturally infected with simian FV type 3 were investigated for the presence of FV proviral DNA and viral transcripts. All organs contained significant amounts of FV proviral DNA. In addition to proviruses containing the complete transactivator gene taf, proviral genomes carrying a specific 295-bp deletion in the taf gene were detected in all monkeys. As in the case of human foamy virus the deletion leads to the formation of the bet gene that is regarded to be instrumental in the regulation of viral persistence. FV RNA was detected by RT-PCR and in situ hybridization only in the oral mucosa of one monkey. No other samples contained detectable levels of viral transcripts. Histopathological changes were not observed in any of the tissue samples analyzed. Our results show that the natural history of FV is characterized by latent infection in all organs of the host and by minimal levels of harmless viral replication in the oral mucosa. The broad host cell range in vivo further encourages the development of FV-derived vectors for therapeutic gene delivery

Molecular dissection of Alzheimer's disease neuropathology by depletion of serum amyloid P component

New therapeutic approaches in Alzheimer's disease are urgently needed. The normal plasma protein, serum amyloid P component (SAP), is always present in cerebrospinal fluid (CSF) and in the pathognomonic lesions of Alzheimer's disease, cerebrovascular and intracerebral Aβ amyloid plaques and neurofibrillary tangles, as a result of its binding to amyloid fibrils and to paired helical filaments, respectively. SAP itself may also be directly neurocytotoxic. Here, in this unique study in Alzheimer's disease of the bis(d-proline) compound, (R)-1-[6-[(R)-2-carboxy-pyrrolidin-1-yl]-6-oxo-hexanoyl]pyrrolidine-2-carboxylic acid (CPHPC), we observed depletion of circulating SAP and also remarkable, almost complete, disappearance of SAP from the CSF. We demonstrate that SAP depletion in vivo is caused by CPHPC cross-linking pairs of SAP molecules in solution to form complexes that are immediately cleared from the plasma. We have also solved the structure of SAP complexed with phosphothreonine, its likely ligand on hyperphosphorylated τ protein. These results support further clinical study of SAP depletion in Alzheimer's disease and potentially other neurodegenerative diseases