Arenavirus reverse genetics: New approaches for the investigation of arenavirus biology and development of antiviral strategies

AbstractSeveral arenaviruses, chiefly Lassa virus, cause hemorrhagic fever disease in humans and pose a significant public health problem in their endemic regions. On the other hand the prototypic arenavirus LCMV is a superb workhorse for the investigation of virus–host interactions and associated disease. The development of novel antiviral strategies to combat pathogenic arenaviruses would be facilitated by a detailed understanding of the arenavirus molecular and cell biology. To this end, the development of reverse genetic systems for several arenaviruses has provided investigators with novel and powerful approaches to dissect the functions of arenavirus proteins and their interactions with host factors required to complete each of the steps of the virus life cycle, as well as to cause disease

The GRB 071112C: A Case Study of Different Mechanisms in X-ray and Optical Temporal Evolution

We present the study on GRB 071112C X-ray and optical light curves. In these two wavelength ranges, we have found different temporal properties. The R-band light curve showed an initial rise followed by a single power-law decay, while the X-ray light curve was described by a single power-law decay plus a flare-like feature. Our analysis shows that the observed temporal evolution cannot be described by the external shock model in which the X-ray and optical emission are produced by the same emission mechanism. No significant color changes in multi-band light curves and a reasonable value of the initial Lorentz factor ({\Gamma}0 = 275 \pm 20) in a uniform ISM support the afterglow onset scenario as the correct interpretation for the early R-band rise. The result suggests the optical flux is dominated by afterglow. Our further investigations show that the X-ray flux could be created by an additional feature related to energy injection and X-ray afterglow. Different theoretical interpretations indicate the additional feature in X-ray can be explained by either late internal dissipation or local inverse-Compton scattering in the external shock.Comment: 20 pages, 3 figures, accepted for publication in Ap

Design and Performance of the Wide-Field X-Ray Monitor on Board the High-Energy Transient Explorer 2

The Wide-field X-ray Monitor (WXM) is one of the scientific instruments carried on the High Energy Transient Explorer 2 (HETE-2) satellite launched on 2000 October 9. HETE-2 is an international mission consisting of a small satellite dedicated to provide broad-band observations and accurate localizations of gamma-ray bursts (GRBs). A unique feature of this mission is its capability to determine and transmit GRB coordinates in almost real-time through the burst alert network. The WXM consists of three elements: four identical Xe-filled one-dimensional position-sensitive proportional counters, two sets of one-dimensional coded apertures, and the main electronics. The WXM counters are sensitive to X-rays between 2 keV and 25 keV within a field-of-view of about 1.5 sr, with a total detector area of about 350 cm$^2$. The in-flight triggering and localization capability can produce a real-time GRB location of several to 30 arcmin accuracy, with a limiting sensitivity of $10^{-7}$ erg cm$^{-2}$. In this report, the details of the mechanical structure, electronics, on-board software, ground and in-flight calibration, and in-flight performance of the WXM are discussed.Comment: 28 pages, 24 figure

Observations of the Prompt Gamma-Ray Emission of GRB 070125

The long, bright gamma-ray burst GRB 070125 was localized by the Interplanetary Network. We present light curves of the prompt gamma-ray emission as observed by Konus-WIND, RHESSI, Suzaku-WAM, and \textit{Swift}-BAT. We detail the results of joint spectral fits with Konus and RHESSI data. The burst shows moderate hard-to-soft evolution in its multi-peaked emission over a period of about one minute. The total burst fluence as observed by Konus is $1.79 \times 10^{-4}$ erg/cm$^2$ (20 keV--10 MeV). Using the spectroscopic redshift $z=1.548$, we find that the burst is consistent with the ``Amati'' $E_{peak,i}-E_{iso}$ correlation. Assuming a jet opening angle derived from broadband modeling of the burst afterglow, GRB 070125 is a significant outlier to the ``Ghirlanda'' $E_{peak,i}-E_\gamma$ correlation. Its collimation-corrected energy release $E_\gamma = 2.5 \times 10^{52}$ ergs is the largest yet observed.Comment: 25 pages, 6 figures; accepted for publication in ApJ. Improved spectral fits and energetics estimate

Optical Classification of Gamma-Ray Bursts in the Swift Era

We propose a new method for the classification of optically dark gamma-ray bursts (GRBs), based on the X-ray and optical-to-X-ray spectral indices of GRB afterglows, and utilizing the spectral capabilities of Swift. This method depends less on model assumptions than previous methods, and can be used as a quick diagnostic tool to identify optically sub-luminous bursts. With this method we can also find GRBs that are extremely bright at optical wavelengths. We show that the previously suggested correlation between the optical darkness and the X-ray/gamma-ray brightness is merely an observational selection effect.Comment: 6 pages, 3 figures; accepted for publication in Ap

Detection of lipid-induced structural changes of the Marburg virus matrix protein VP40 using hydrogen/deuterium exchange-mass spectrometry

Marburg virus (MARV) is a lipid-enveloped virus from the Filoviridae family containing a negative sense RNA genome. One of the seven MARV genes encodes the matrix protein VP40, which forms a matrix layer beneath the plasma membrane inner leaflet to facilitate budding from the host cell. MARV VP40 (mVP40) has been shown to be a dimeric peripheral protein with a broad and flat basic surface that can associate with anionic phospholipids such as phosphatidylserine. Although a number of mVP40 cationic residues have been shown to facilitate binding to membranes containing anionic lipids, much less is known on how mVP40 assembles to form the matrix layer following membrane binding. Here we have used hydrogen/deuterium exchange (HDX) mass spectrometry to determine the solvent accessibility of mVP40 residues in the absence and presence of phosphatidylserine and phosphatidylinositol 4,5-bisphosphate. HDX analysis demonstrates that two basic loops in the mVP40 C-terminal domain make important contributions to anionic membrane binding and also reveals a potential oligomerization interface in the C-terminal domain as well as a conserved oligomerization interface in the mVP40 N-terminal domain. Lipid binding assays confirm the role of the two basic patches elucidated with HD/X measurements, whereas molecular dynamics simulations and membrane insertion measurements complement these studies to demonstrate that mVP40 does not appreciably insert into the hydrocarbon region of anionic membranes in contrast to the matrix protein from Ebola virus. Taken together, we propose a model by which association of the mVP40 dimer with the anionic plasma membrane facilitates assembly of mVP40 oligomers

Status of GRB Observations with the Suzaku Wideband All-sky Monitor

The Wide-band All-sky Monitor (WAM) is a function of the large lateral BGO shield of the Hard X-ray Detector (HXD) onboard Suzaku. Its large geometrical area of 800 cm^2 per side, the large stopping power for the hard X-rays and the wide-field of view make the WAM an ideal detector for gamma-ray bursts (GRBs) observations in the energy range of 50-5000 keV. In fact, the WAM has observed 288 GRBs confirmed by other satellites, till the end of May 2007.Comment: 4 pages, 4 figures, to be published in the proceedings of ''Gamma Ray Bursts 2007'', Santa Fe, New Mexico, November 5-

Resilience and economic empowerment: A qualitative investigation of entrepreneurial Indonesian Women

The development of female entrepreneurs in Indonesia is an integral part of Muslim women\u27s economic contributions and empowerment. However, there is a lack of reliable research about female entrepreneurship and how gender may affect the experiences of business ownership in Indonesia. Therefore, the aim of this study is to explore the challenges encountered by these women entrepreneurs on a daily basis. Qualitative in-depth interviews were conducted with 30 female Indonesian entrepreneurs. Participants were recruited using theoretical and maximum variation sampling techniques. Content analysis was then used to analyze the data. Results revealed high levels of variations, both within and between women, suggesting that the quality of business entrepreneurship and success depended largely on the personal characteristics of these women, rather than on any system of formal education or training. This study also found that many women displayed resilient coping strategies when dealing with business failures. As a consequence, they were able to thrive despite restrictive social, cultural and political constraints. The paper highlights the importance of the experiences of female entrepreneurs in a developing country and the need to integrate the development of female entrepreneurship as a part of women empowerment effort

Swift and Suzaku Observations of the X-Ray Afterglow from the GRB 060105

Results are presented of early X-ray afterglow observations of GRB 060105 by Swift and Suzaku. The bright, long gamma-ray burst GRB 060105 triggered the Swift Burst Alert Telescope (BAT) at 06:49:28 on 5 January 2006. The Suzaku team commenced a pre-planned target of opportunity observation at 19 ks (5.3 hr) after the Swift trigger. Following the prompt emission and successive very steep decay, a shallow decay was observed from T_0+187 s to T_0+1287 s. After an observation gap during T_0 +(1.5-3) ks, an extremely early steep decay was observed in T_0+(4-30) ks. The lightcurve flattened again at T_0+30 ks, and another steep decay followed from T_0+50 ks to the end of observations. Both steep decays exhibited decay indices of 2.3 - 2.4. This very early break, if it is a jet break, is the earliest case among X-ray afterglow observations, suggesting a very narrow jet whose opening angle is well below 1 degree. The unique Suzaku/XIS data allow us to set very tight upper limits on line emission or absorption in this GRB. For the reported pseudo-redshift of z=4.0+/-1.3 the upper limit on the iron line equivalent width is 50 eV.Comment: 8 pages, 5 figures, Accepted for publication in PASJ Suzaku Special Issue (vol. 58