Brief communication: impacts of ocean-wave-induced breakup of Antarctic sea ice via thermodynamics in a stand-alone version of the CICE sea-ice model

Impacts of wave-induced breakup of Antarctic sea ice on ice concentration and volume are investigated using a modified version of the CICE sea-ice model, run in standalone mode from 1979–2010. Model outputs show that during summer wave-induced breakup reduces local ice concentration by up to 0.3–0.4 in a vicinity of the ice edge and total ice volume by up to a factor of 0.1–0.2.Luke G. Bennetts, Siobhan O’Farrell, and Petteri Uotil

Atmospheric Circulation Response to Anomalous Siberian Forcing in October 2016 and its Long‐Range Predictability

Abstract: The warm Arctic-cold continent pattern was of record strength in October 2016, providing the opportunity to test its proposed influence on large-scale atmospheric circulation. We find a record weak polar stratospheric vortex and negative North Atlantic Oscillation in November-December 2016 and link them to increased planetary wave generation associated with cold Siberian anomalies followed by troposphere-stratosphere dynamical coupling. At the same time the warm Arctic anomalies, in particular those over the Barents-Kara Seas, do not appear to play an important role in forcing the atmospheric circulation. Long-range forecasts initialized on 1 October 2016 reproduced both the weak polar vortex and negative North Atlantic Oscillation, as well as their link with the Siberian temperatures. Our results support the stratospheric pathway for atmospheric circulation forcing associated with Siberian surface anomalies and uncover a source of skill for subseasonal forecasts from October to December. Plain Language Summary: The warm Arctic-cold continent pattern is an observed, large-scale pattern of near-surface temperatures where the Arctic is warmer than average and Siberia is colder than average. This pattern was of record strength in October 2016, providing the opportunity to test its influence on the Northern Hemisphere atmospheric circulation and the possibility of skillful long-range forecasts. It has been proposed that the warm Arctic-cold continent pattern can drive large atmospheric waves, which are able to travel from the troposphere into the stratosphere, where they weaken the strong wintertime winds that make up the stratospheric polar vortex. A weakened polar vortex can then lead to changes in the surface pressure that can affect weather patterns. We find a record weak polar stratospheric vortex in late autumn 2016 and link that to cold Siberian anomalies. At the same time the warm Arctic anomalies do not appear to play an important role in forcing the atmospheric circulation. Long-range forecasts initialized in October 2016 reproduced both the weak polar vortex and resulting surface pressure patterns. Our results support the stratospheric pathway for atmospheric circulation forcing by Siberian surface anomalies and uncover a source of skill for subseasonal forecasts in the Northern Hemisphere autumn.Peer reviewe

Subpolar Southern Ocean response to changes in the surface momentum, heat, and freshwater fluxes under 2xCO2

The Antarctic subpolar Southern Ocean (sSO) has fundamental climate importance. Antarctic Bottom Water (AABW) originates in the sSO and supplies the lower limb of the meridional overturning circulation (MOC), occupying 36% of ocean volume. Climate models struggle to represent continental shelf processes that form AABW. We explore sources of persistent model biases by examining response of the sSO to perturbations in surface forcing in a global ocean–sea ice model (ACCESS-OM2) that forms AABW both on shelf and in open ocean. The sSO response to individual and combined perturbations of surface heat, freshwater, and momentum fluxes follows the WCRP CMIP6 FAFMIP-protocol. Wind perturbation (i.e., a poleward shift and intensification of the westerlies) is dominant, enhancing AABW formation and accelerating the global MOC. This occurs through upwelling of warm waters and inhibition of sea ice growth during winter, which triggers large open water polynya (OWP) events with associated deep convection. These events occur in the Weddell and Ross Seas and their variability is associated with availability of heat at midocean depths. These OWPs cease when the heat reservoir is depleted. Effects of surface warming and freshening only partially compensate changes from increasing winds on ocean stratification and depletion of AABW formation. These results indicate that overly convective models, such ACCESS-OM2, can respond to CO2-perturbed scenarios by forming too much AABW in OWP, which might not hold in models without OWPs. This might contribute to the large intermodel spread thermosteric sea level projections, being relevant to the interpretation of future projections by current climate models.Peer reviewe

Sub-ppb detection of formaldehyde with cantilever enhanced photoacoustic spectroscopy using quantum cascade laser source

Abstract A novel cantilever enhanced photoacoustic spectrometer with mid-infrared quantum cascade laser was applied for selective and sensitive formaldehyde (CH 2 O) gas measurement. The spectrum of formaldehyde was measured from 1,772 to 1,777 cm -1 by tuning the laser with a spectral resolution of 0.018 cm -1 . The band at 1,773.959 cm -1 was selected for data analysis, at which position the laser emitted 47 mW. In univariate measurement, the detection limit (3r, 0.951 s) and the normalized noise equivalent absorption coefficient (3r) for amplitude modulation (AM) were 1.6 ppbv and 7.32 9 10 -10 W cm -1 (Hz) -1/2 and for wavelength modulation (WM) 1.3 ppbv and 6.04 9 10 -10 W cm -1 (Hz) -1/2 . In multivariate measurement, the detection limit (3r) can be as low as 901 pptv (1,773.833-1,774.085 cm -1 , 15 spectral points each 0.951 s) for AM and 623 pptv (1,773.743-1,774.265 cm -1 , 30 spectral points each 0.951 s) for WM. Because measurement time increases in multivariate measurement, its application is justified only when interferents need to be resolved. Potential improvements of the system are discussed

A beta 2-Integrin/MRTF-A/SRF Pathway Regulates Dendritic Cell Gene Expression, Adhesion, and Traction Force Generation

beta 2-integrins are essential for immune system function because they mediate immune cell adhesion and signaling. Consequently, a loss of beta(2)-integrin expression or function causes the immunodeficiency disorders, Leukocyte Adhesion Deficiency (LAD) type I and III. LAD-III is caused by mutations in an important integrin regulator, kindlin-3, but exactly how kindlin-3 regulates leukocyte adhesion has remained incompletely understood. Here we demonstrate that mutation of the kindlin-3 binding site in the beta 2-integrin (TTT/AAA-beta 2-integrin knock-in mouse/KI) abolishes activation of the actin-regulated myocardin related transcription factor A/serum response factor (MRTF-A/SRF) signaling pathway in dendritic cells and MRTF-A/SRF-dependent gene expression. We show that Ras homolog gene family, member A (RhoA) activation and filamentous-actin (F-actin) polymerization is abolished in murine TTT/AAA-beta 2-integrin KI dendritic cells, which leads to a failure of MRTF-A to localize to the cell nucleus to coactivate genes together with SRF. In addition, we show that dendritic cell gene expression, adhesion and integrin-mediated traction forces on ligand coated surfaces is dependent on the MRTF-A/SRF signaling pathway. The participation of beta 2-integrin and kindlin-3-mediated cell adhesion in the regulation of the ubiquitous MRTF-A/SRF signaling pathway in immune cells may help explain the role of beta 2-integrin and kindlin-3 in integrin-mediated gene regulation and immune system function

Dysfunction of complement receptors CR3 (CD11b/18) and CR4 (CD11c/18) in pre-eclampsia : a genetic and functional study

Objective To study genetic variants and their function within genes coding for complement receptors in pre-eclampsia. Design A case-control study. Setting Pre-eclampsia is a common vascular disease of pregnancy. The clearance of placenta-derived material is one of the functions of the complement system in pregnancy. Population We genotyped 500 women with pre-eclamptic pregnancies and 190 pregnant women without pre-eclampsia, as controls, from the FINNPEC cohort, and 122 women with pre-eclamptic pregnancies and 1905 controls from the national FINRISK cohort. Methods The functional consequences of genotypes discovered by targeted exomic sequencing were explored by analysing the binding of the main ligand iC3b to mutated CR3 or CR4, which were transiently expressed on the surface of COS-1 cells. Main outcome measures Allele frequencies were compared between pre-eclamptic pregnancies and controls in genetic studies. The functional consequences of selected variants were measured by binding assays. Results The most significantly pre-eclampsia-linked CR3 variant M441K (P = 4.27E-4, OR = 1.401, 95% CI = 1.167-1.682) displayed a trend of increased adhesion to iC3b (P = 0.051). The CR4 variant A251T was found to enhance the adhesion of CR4 to iC3b, whereas W48R resulted in a decrease of the binding of CR4 to iC3b. Conclusions Results suggest that changes in complement-facilitated phagocytosis are associated with pre-eclampsia. Further studies are needed to ascertain whether aberrant CR3 and CR4 activity leads to altered pro- and anti-inflammatory cytokine responses in individuals carrying the associated variants, and the role of these receptors in pre-eclampsia pathogenesis. Tweetable abstract Genetic variants of complement receptors CR3 and CR4 have functional consequences that are associated with pre-eclampsia.Peer reviewe

Mice Lacking beta2-Integrin Function Remain Glucose Tolerant in Spite of Insulin Resistance, Neutrophil Infiltration and Inflammation

Beta2-integrins are important in leukocyte trafficking and function, and are regulated through the binding of cytoplasmic proteins, such as kindlin-3, to their intracellular domain. Here, we investigate the involvement of beta2-integrins in the regulation of metabolic disease using mice where the kindlin-3 binding site in the beta2-integrin cytoplasmic tail has been mutated (TTT/AAA-beta2-integrin knock-in (KI) mice), leading to expressed but dysfunctional beta2-integrins and significant neutrophilia in vivo. Beta2-integrin KI mice fed on a high fat diet showed normal weight gain, and normal accumulation of macrophages and lymphocytes in white adipose tissue (WAT) and liver, but increased neutrophil numbers especially in WAT. In addition, beta2-integrin KI mice fed on a high fat diet showed significantly increased peripheral insulin resistance in response to high-fat feeding. However, this was associated with improved glucose disposal following glucose load. Interestingly, beta2-integrin KI neutrophils produced more elastase in vitro, in response to stimulation. Beta2-integrin KI mice displayed variability of tissue inflammatory status, with liver and WAT exhibiting little or no difference in inflammation compared to high fat fed controls, whereas skeletal muscle demonstrated a raised inflammatory profile in association with higher elastase levels and diminished signalling through the IRS1-PKB pathway. In conclusion, although expression of dysfunctional beta2-integrins increased neutrophil production and infiltration into tissue, skeletal muscle was the most affected tissue exhibiting evidence of higher neutrophil activity and insulin resistance. Thus, beta2-integrins modulate glucose homeostasis during high fat feeding predominantly through actions on skeletal muscle to affect metabolic phenotype in vivo.Peer reviewe

Passage and concentration-dependent effects of Indomethacin on tendon derived cells

<p>Abstract</p> <p>Background</p> <p>Non-steroidal anti-inflammatory drugs (NSAID) are commonly used in the treatment of tendinopathies such as tendonitis and tendinosis. Despite this, little is known of their direct actions on tendon-derived cells. As NSAIDs have been shown to delay healing in a number of mesenchymal tissues we have investigated the direct effects of indomethacin on the proliferation of tendon-derived cells.</p> <p>Results and Discussion</p> <p>The results obtained were dependent on both the type of cells used and the method of measurement. When measured using the Alamar blue assay, a common method for the measurement of cell proliferation and viability, no effect of indomethacin was seen regardless of cell source. It is likely that this lack of effect was due to a paucity of mitochondrial enzymes in tendon cells.</p> <p>However, when cell number was assessed using the methylene blue assay, which is a simple nuclear staining technique, an Indomethacin-induced inhibition of proliferation was seen in primary cells but not in secondary subcultures.</p> <p>Conclusion</p> <p>These results suggest that firstly, care must be taken when deciding on methodology used to investigate tendon-derived cells as these cells have a quite different metabolism to other mesenchymal derive cells. Secondly, Indomethacin can inhibit the proliferation of primary tendon derived cells and that secondary subculture selects for a population of cells that is unresponsive to this drug.</p

What is the potential for replacing monocultures with mixed-species stands to enhance ecosystem services in boreal forests in Fennoscandia?

The boreal forests of Fennoscandia are largely dominated by Norway spruce and Scots pine. Conifer monocultures have been favoured in forest management during the last decades. Recently, concern has risen that forests consisting of only one tree species could be vulnerable to biotic damage. Additionally, environmental and societal changes are placing new demands on forest utilization, thus shifting the focus to alternative forest management options providing a wider scale of ecosystem services. It has been proposed that mixed forests are better than monocultures with respect to biodiversity, risk management and recreational value. By synthesising research studies, we provide an overview of current knowledge on how to combine wood production and other ecosystem services in mixed boreal forests in Fennoscandia. We addressed the following questions in more detail: what are the effects of mixed forests on soil properties, understorey vegetation, biodiversity, wildlife, resistance to and resilience against damage, forest productivity and the multiple use of forests? Furthermore, what are the silvicultural possibilities for establishing and managing mixed forests?Based on this review, mixed forests appear to provide a higher output of most ecosystem goods and services, including higher biodiversity and improved risk management, soil properties and multiple-use values. The most serious challenge is the browsing by cervids, which damages sapling stands. There is potential to establish single-storied mixed forests with current regeneration methods and material. Further research is particularly needed on the silvicultural practices suited for mixed boreal forests

COX2 genetic variation, NSAIDs, and advanced prostate cancer risk

Collective evidence suggests that cyclooxygenase 2 (COX2) plays a role in prostate cancer risk. Cyclooxygenase 2 is the major enzyme that converts arachidonic acid to prostaglandins, which are potent mediators of inflammation. Nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit the enzymatic activity of COX2 and long-term use of NSAIDs appears to modestly lower the risk of prostate cancer. We investigated whether common genetic variation in COX2 influences the risk of advanced prostate cancer. Nine single-nucleotide polymorphisms (SNPs) in COX2 were genotyped among 1012 men in our case–control study of advanced prostate cancer. Gene–environment interactions between COX2 polymorphisms and NSAID use were also evaluated. Information on NSAID use was obtained by questionnaire. Three SNPs demonstrated nominally statistically significant associations with prostate cancer risk, with the most compelling polymorphism (rs2745557) associated with a lower risk of disease (odds ratio (OR) GC vs GG=0.64; 95% confidence interval (CI): 0.49–0.84; P=0.002). We estimated through permutation analysis that a similarly strong result would occur by chance 2.7% of the time. Nonsteroidal anti-inflammatory drug use was associated with a lower risk of disease in comparison to no use (OR=0.67; 95% CI: 0.52–0.87). No significant statistical interaction between NSAID use and rs2745557 was observed (P=0.12). Our findings suggest that variation in COX2 is associated with prostate cancer risk