Stochastic Modelling of Subcellular Biochemical Systems

Stochastic approaches are needed for modelling many cellular processes to capture noise effects. The difficulty of solving the chemical master equation, the most common formulation of stochastic models, is circumvented by stochastic simulations and analytical approximations. The central theme here is one such approximation, the two-moment approximation (2MA) which represents the mean-covariance coupling. Our 2MA formulation allows non-elementary reactions and relative concentrations. The approach is applied to the fission yeast cell cycle model. The analytical model reproduces the relevant experimental data.Die Modellierung zellulärer Prozesse erfordert oft die Anwendung von stochastischen Methoden. Das Problem, die master equation für biochemische Systeme zu lösen kann auf zweierlei Weise umgangen werden. Entweder durch stochastische Simulationen oder durch analytische Approximation. Das Herzstück hier ist die Entwicklung einer solchen Approximation, der zwei-Momenten Approximation, welcher die Verknüpfung von Mittelwert und Kovarianz repräsentiert und es erlaubt Modelle mit nicht elementaren Reaktionen sowie relativen Konzentrationen zu analysieren. Dieser neue Ansatz , angewandt auf ein anerkanntes Modell des Zellzyklus in der Bäckerhefe, reproduziert vorhandene experimentell Daten

Towards autonomy in agriculture: Design and prototyping of a robotic vehicle with seed selector

Traditional method of seeding by farmers demands laborious work and is now becoming story of the past. Technological revolution in mechatronics and allied areas is reshaping the agricultural processes, making the robots an integral part of this automation. This paper presents design details of an autonomous robot developed keeping in view the constraints imposed by an agricultural field. The novelty of the proposed low-cost indigenously developed modular vehicle lies in design of its seed selector. The simple but efficient mechanism of the single seed selector with extremely low miss rate distinguishes the vehicle from other designs. Hardware details including sensing, actuation, processing and communication modules and software architecture are detailed in the paper. Results of trajectory tracking obtained by implementing the proposed scheme on a mini-robot and functionality of seed selector demonstrate potential of the presented robotic vehicle

Percutaneous Intervention or Bypass Graft for Left Main Coronary Artery Disease? A Systematic Review and Meta-Analysis

Background. The safety and efficacy of percutaneous coronary intervention (PCI) versus coronary artery bypass grafting (CABG) for stable left main coronary artery disease (LMCAD) remains controversial.Methods. Digital databases were searched to compare the major adverse cardiovascular and cerebrovascular events (MACCE) and its components. A random effect model was used to compute an unadjusted odds ratio (OR).Results. A total of 43 studies (37 observational and 6 RCTs) consisting of 29,187 patients (PCI 13,709 and CABG 15,478) were identified. The 30-day rate of MACCE (OR, 0.56; 95% CI, 0.42-0.76;p = 0.0002) and all-cause mortality (OR, 0.52; 95% CI, 0.30-0.91;p = 0.02) was significantly lower in the PCI group. There was no significant difference in the rate of myocardial infarction (MI) (p = 0.17) and revascularization (p = 0.12). At 5 years, CABG was favored due to a significantly lower rate of MACCE (OR, 1.67; 95% CI, 1.18-2.36;p = <0.04), MI (OR, 1.67; 95% CI, 1.35-2.06;p = <0.00001), and revascularization (OR, 2.80; 95% CI, 2.18-3.60;p = <0.00001), respectively. PCI was associated with a lower overall rate of a stroke, while the risk of all-cause mortality was not significantly different between the two groups at 1- (p = 0.75), 5- (p = 0.72), and 10-years (p = 0.20). The Kaplan-Meier curve reconstruction revealed substantial variations over time; the 5-year incidence of MACCE was 38% with CABG, significantly lower than 45% with PCI (p = <0.00001).Conclusion. PCI might offer early safety advantages, while CABG provides greater durability in terms of lower long-term risk of ischemic events. There appears to be an equivalent risk for all-cause mortality

Petrographic and Physiomechanical Investigation of Late Cretaceous Kawagarh Formation Kahi Section, Nizampur Basin

The late Cretaceous Kawagarh Formation has been investigated in terms of field observation, and petrographic analysis, to understand the petrography and its impact on the geotechnical properties. The Kawagarh Formation is well exposed among the upper Indus Basin, and has been studied by various workers in different aspects. Kawagarh Formationexposed in Kahi section of Nizampur Basin has been selected in this study to know the behavior of carbonate rocks for engineering purposes. Lithologically, this formation is composed of thick to medium bedded, highly fractured limestone, marls, and dolomitic limestone which has undertaken diagenetic alteration including dolomite, calcite veins, and stylolites. Followed by petrographic analysis which reveals that the Kawagarh limestone is mostly fossiliferouscomprised of a large number of planktonic foraminifera fossils like Globotruncana Hilli and Globotruncana Linneana fossils. Furthermore, to know the impact of petrographic minerals on engineering behavior, mechanical properties in terms of uniaxial compressive strength (UCS) and uniaxial tensile strength (UTS) were also computed by using a universal testing machine (UTM). The resultant mechanical values lie in the strong compressive strength and suggest their usage for various construction purposes. Aggregate degradation tests including water absorption, specific gravity, aggregate impact value, Los angles abrasion, and soundness was also computed according to the International standard organization, ASTM (American Society for testing materials) and British standard. The aggregate values of the Cretaceous Kawagarh Formation are within the defined standard limits and can be used as an aggregate source for different construction engineering projects

Livestock in Pakistan: An Insight into Climate Changes and Impacts

The global livestock sector is expanding at a faster rate than any other agricultural sub-sector. It employs approximately 1.3 billion people and accounts for approximately 40 % of global agricultural output. This industry is one of the most damaging sectors to the world\u27s increasingly scarce water resources, contributing to water pollution from animal wastes, hormones, antibiotics, chemicals from tanneries, pesticides, and fertilizers used to spray feed crop. Climate change harms livestock productivity by changing ecosystem services i.e. water availability, forage quality and quantity, diseases outbreak, and animals stress due to heat shock and reduction of livestock diversity and breeds. Ecosystem and animal health are under the direct influence of climate change. The prevalence and dispersal of animal illnesses and pathogens are presently on the rise due to climate change. Climate change may have a significant impact on farm animal production performance around the world. Heat stress appears to be one of the intriguing environmental variables affecting animals, making animal production challenging in many geographical locations around the world. Intake of feed can decrease at high temperatures while energy demands increase due to the activation of thermoregulation mechanisms, which harms productivity, growth, and development. This is because thermoregulation mechanisms are activated when temperatures are high. The rate at which animals digest their food is impacted, subjected to heat stress, in addition to their rate of growth and development. The animal\u27s heart rate, rectal temperature, and respiratory rate can all be increased during expose to environments with high temperatures

Knowledge, Attitude and Practice Regarding Exclusive Breastfeeding Among Mothers Attending Tertiary Care Hospital

OBJECTIVES: To determine knowledge, attitude, and practice regarding exclusive breastfeeding among mothers attending tertiary care hospitals.  METHODOLOGY: A descriptive cross-sectional study was conducted at the Department of Pediatrics in Hayatabad Medical Complex, Peshawar. A total of 162 mothers were selected who were practicing breastfeeding of ages between 20 to 35 years, having a full-term baby of age up to 6 months old. The data is collected through a pre-planned questionnaire and then was analyzed using statistics version 24.0. The duration of the study was from 1st June 2020 to 30th October 2020. RESULTS: The results showed that no significant association was found between age groups, socio-economic status regarding knowledge, attitude, and practice (p&gt;0.05). However, there was a significant difference between knowledge, attitude, and practice with respect to educational status (p&lt;0.05). 123 (73.21%) mothers had unsatisfactory; whereas 39 (24.07%) mothers had excellent knowledge regarding breastfeeding. 39 (27.07%) of breast-feeding mothers had a positive attitude towards breastfeeding. 123 (73.21%) women showed a negative response regarding the practice of breastfeeding. CONCLUSION: Most of the mothers didn’t have adequate knowledge of exclusive breastfeeding (EBF) practice. As a result, it is suggested that media can be used as a medium to educate women about the benefits of exclusive breastfeeding (EBF)

Microfacies Analysis and Depositional Environment of Middle Jurassic Samana Suk Formation, Chichali Nala Section, Surghar Range, Pakistan

The Middle Jurassic age Samana Suk Formation, exposed in Chichali Nala section of Surghar ranges has been investigated by field work, petrographic study and XRD analysis to understand the microfacies, depositional environment and fault related dolomitization of the Samana Suk Formation. This formation is widely distributed in the upper Indus basin of Pakistan and considered the most prominent stratigraphic unit of the Jurassic period. The project area lies in the Chichali Nala Section of Surghar range (Trans Indus Salt Ranges). In this section, Samana Suk Formation constitutes the lithology of carbonate having CaCo3 as a major mineral, where dolomite is present in minor amount, which is restricted to fluids along fault zone. During the study two major microfacies have been identified including the Grainstone microfacies and Mudstone-Wackestone microfacies. Samana Suk. Formation was formed under stormy influence in the environment of deposition of Formation. Its depositional environment is the inner-middle shelf which suggests the marine shelf depositional environment