Photo-strobo-acoustic Imaging at the Microscale by Laser-Induced Ultrasound

The combination of microfluidic technology and optical fluids characterization techniques has been recently applied to produce lab-on-a-chip systems. In the present work, bringing together the imaging technique called photoacoustic imaging (PAI) and microfluidic technology were implemented to obtain micro-scale imaging. Laser-induced ultrasound signals were measured from microdroplets produced in a simple T-junction microfluidic system. Single pulse laser images were produced as a result of the combination of the aforementioned techniques, allowing to obtain of geometrical information of the microdroplets and its spatial position.Comment: Manuscript in proces

Duplicación de colon y recto. Presentación de un caso clínico.

Introducción: Las duplicaciones intestinales tienen una incidencia de dos por cada 10,000 nacidos vivos. Con frecuencia, el diagnóstico se establece en la intervención quirúrgica. Objetivo: Presentar un caso clínico de duplicación intestinal y revisión de la bibliografía. Caso clínico: RN sexo femenino a término, sin antecedentes de importancia con diversas anomalías encontradas a la exploración física, destacando escoliosis dorsal, masa pélvica y otras alteraciones urogenitales. Conclusión: Se diagnosticó duplicación de colon y recto, acompañado de duplicación urogenital y hemivértebras. Se resecaron colon y recto duplicados y masa pélvica, con buena evolución

Usporedba djelovanja korionskog gonadotropina konja (eCG) i estradiol cipionata primijenjenih 24 sata nakon prestanka kontrolirane primjene progesterona kao dijela protokola za sinkronizaciju estrusa i umjetno osjemenjivanje meksičkog Criollo goveda.

Two experiments were conducted to evaluate the effect of administering equine chorionic gonadotropin (eCG) in substitution of estradiol, 24 h after the removal of a progesterone intravaginal device, on estrus and ovulatory response, and pregnancy rates of Rodeo Criollo cows (exp. 1, n = 21) and heifers (exp. 2, n = 39) subjected to a synchronization protocol (estradiol + CIDR + PGF2α) with estrus-detected artificial insemination. All females were inseminated 12 h after detected estrus. In cows, estrus and ovulation response, and maximum pre-ovulatory follicle diameter were similar (P>0.05) between equine chorionic gonadotropin and estradiol groups. However, the time to estrus was shorter (P0.05) in ovulation rate. Both treatments resulted in low pregnancy rates, with a significantly lower (P0,05) u skupinama krava kod kojih je bio primijenjen korionski gonadrotropin ili estradiol. Međutim, u skupini kod koje je bio primijenjen estradiol vrijeme do estrusa je bilo kraće (P0,05) u stopi ovulacije. Oba su postupka dovela do niže stope gravidnosti, pri čemu je smanjenje kod junica kojima je bio primijenjen korionski gonadotropin konja bilo signifikantno (P<0,05). Zaključno, primjena korionskog gonadotropina konja, kao zamjene za estradiol nakon uklanjanja sredstva za otpuštanje progesterona, dovela je u sklopu protokola za otkrivanje estrusa i umjetno osjemenjivanje do povećanog grupiranja estrusa i ovulacije te posljedično veće stope gravidnosti u krava, ali ne i u junica

Usporedba djelovanja korionskog gonadotropina konja (eCG) i estradiol cipionata primijenjenih 24 sata nakon prestanka kontrolirane primjene progesterona kao dijela protokola za sinkronizaciju estrusa i umjetno osjemenjivanje meksičkog Criollo goveda.

Two experiments were conducted to evaluate the effect of administering equine chorionic gonadotropin (eCG) in substitution of estradiol, 24 h after the removal of a progesterone intravaginal device, on estrus and ovulatory response, and pregnancy rates of Rodeo Criollo cows (exp. 1, n = 21) and heifers (exp. 2, n = 39) subjected to a synchronization protocol (estradiol + CIDR + PGF2α) with estrus-detected artificial insemination. All females were inseminated 12 h after detected estrus. In cows, estrus and ovulation response, and maximum pre-ovulatory follicle diameter were similar (P>0.05) between equine chorionic gonadotropin and estradiol groups. However, the time to estrus was shorter (P0.05) in ovulation rate. Both treatments resulted in low pregnancy rates, with a significantly lower (P0,05) u skupinama krava kod kojih je bio primijenjen korionski gonadrotropin ili estradiol. Međutim, u skupini kod koje je bio primijenjen estradiol vrijeme do estrusa je bilo kraće (P0,05) u stopi ovulacije. Oba su postupka dovela do niže stope gravidnosti, pri čemu je smanjenje kod junica kojima je bio primijenjen korionski gonadotropin konja bilo signifikantno (P<0,05). Zaključno, primjena korionskog gonadotropina konja, kao zamjene za estradiol nakon uklanjanja sredstva za otpuštanje progesterona, dovela je u sklopu protokola za otkrivanje estrusa i umjetno osjemenjivanje do povećanog grupiranja estrusa i ovulacije te posljedično veće stope gravidnosti u krava, ali ne i u junica

Centro de diseño y patronaje para la industria del calzado de las MYPES de El Salvador

Se proponen directrices generales relacionadas con el “Diseño y Patronaje de Calzado” mediante la ayuda de CONAMYPE y las asociaciones de calzado de El Salvador, donde, se identifica sus necesidades relacionadas al Diseño y Patronaje de Calzado y un diagnóstico minucioso sobre las condiciones actuales en las cuales se encuentran trabajando, y además un diseño del centro de Diseño y Patronaje acorde al diagnostico realizad

Resistance and inactivation kinetics of bacterial strains isolated from the Non-chlorinated and chlorinated effluents of a WWTP

The microbiological quality of water from a wastewater treatment plant that uses sodium hypochlorite as a disinfectant was assessed. Mesophilic aerobic bacteria were not removed efficiently. This fact allowed for the isolation of several bacterial strains from the effluents. Molecular identification indicated that the strains were related to Aeromonas hydrophila, Escherichia coli (three strains), Enterobacter cloacae, Kluyvera cryocrescens (three strains), Kluyvera intermedia, Citrobacter freundii (two strains), Bacillus sp. and Enterobacter sp. The first five strains, which were isolated from the non-chlorinated effluent, were used to test resistance to chlorine disinfection using three sets of variables: disinfectant concentration (8, 20 and 30 mg·L−1), contact time (0, 15 and 30 min) and water temperature (20, 25 and 30 °C). The results demonstrated that the strains have independent responses to experimental conditions and that the most efficient treatment was an 8 mg·L−1 dose of disinfectant at a temperature of 20 °C for 30 min. The other eight strains, which were isolated from the chlorinated effluent, were used to analyze inactivation kinetics using the disinfectant at a dose of 15 mg·L−1 with various retention times (0, 10, 20, 30, 60 and 90 min). The results indicated that during the inactivation process, there was no relationship between removal percentage and retention time and that the strains have no common response to the treatmentsThe work of SM-H was supported by a graduate scholarship (number 217745) that was kindly provided by CONACyT, Mexico. Some chemical reagents were generously provided by the Administration of the B.A. in Biology at UAEH, Mexico. We thank the Instituto Tecnológico de Estudios Superiores de Monterrey, Hidalgo campus, for allowing us to sample from its WWTP. The authors recognize Jose A. Rodriguez-Ávila for his comments on the procedure for analyzing inactivation kineticsS

Genome misclassification of Klebsiella variicola and Klebsiella quasipneumoniae isolated from plants, animals and humans

Objective. Due to the fact that K. variicola, K. quasipneu­moniae and K. pneumoniae are closely related bacterial species, misclassification can occur due to mistakes either in normal biochemical tests or during submission to public databases. The objective of this work was to identify K. variicola and K. quasipneumoniae genomes misclassified in GenBank database. Materials and methods. Both rpoB phylogenies and average nucleotide identity (ANI) were used to identify a significant number of misclassified Klebsiella spp. genomes. Results. Here we report an update of K. variicola and K. Quasipneumoniae genomes correctly classified and a list of isolated genomes obtained from humans, plants, animals and insects, described originally as K. pneumoniae or K. variicola, but known now to be misclassified. Conclusions. This work contributes to recognize the extensive presence of K. variicola and K. quasipneumoniae isolates in diverse sites and samples

Evaluation of a telepsychological intervention guide during COVID-19 pandemic: evidence from a pilot study with mexican population

La Guía de Atención Psicológica Virtual (GAPV_COVID 19) se ha diseñado como una herramienta de orientación y atención urgente a padecimientos y síntomas psicológicos presentes durante la pandemia por SARS-CoV-2. En el presente estudio, se buscó evaluar el impacto psicológico en los niveles de depresión, ansiedad y estrés asociados a la implementación de una intervención en telepsicología basada en la GAPV_COVID 19. Se diseñó un grupo único de medidas repetidas para evaluar depresión, ansiedad y estrés antes (T0 = pretest), después de la intervención (T1 = posttest ) y 14 días después de su implementación (T2 = retest). Participaron 117 adultos (edad promedio = 37.69; DE = 12.07; 62.4 % varones) que recibieron atención por telepsicología y que fueron canalizados según los criterios de la GAPV_COVID 19 para la atención de sus necesidades psicológicas: nivel 2 psicoeducación y orientación (n = 46), nivel 3 = primeros auxilios psicológicos (n = 24) y nivel 4 = terapia cognitiva conductual breve (n = 47). Se encontraron diferencias significativas en depresión, ansiedad y estrés, para los tres niveles de atención con respecto a las mediciones pre, post y retest, con una magnitud considerable.El presente trabajo involucra un proyecto financiado por el Consejo Nacional de Ciencia y Tecnología (Conacyt) México. Proyecto No. 000000000312728