ФРАКЦІЙНИЙ СКЛАД БІЛКІВ У ПАСТАХ ДЛЯ ДИТЯЧОГО ХАРЧУВАННЯ

The paper presents the results of determination of fractional composition of proteins in the pastes for infant food. It was shown that the use of thermostatic production method for technology of protein pastes for infant food and developed starter compositions of monocultures B. animalis Bb-12 and mixed cultures of Lactococcus lactis ssp. with heightened proteolytic properties as part of the frozen bacterial concentrates F DVS C-303 (or F DVS C -303) or of concentrates of FD DVS CH N-11 (or FD-DVS CH N 19 or CH N DVS FD-22 or FD-DVS Flora danica), prepared by freeze-drying, allows to obtain products with hypoallergenic properties. The article specifies that the protein pastes for infant food enriched with lactulose, a complex of vitamins FT 041081EU and a complex of minerals FT 042836EU have the highest hypoallergenic properties: the content of α- and β-casein in the experimental samples of products produced using above mentioned starter compositions is 5,24...5,72 and 4,99...5,21 %, respectively, the content of κ-casein complex+β-lactoglobulin – 1,13...1,42 %.  В статье приведены результаты определения фракционного состава белков в пастах для детского питания. Показано, что использование в технологии белковых паст для детского питания термостатного способа производства и разработанных заквасочных композиций из монокультур B. animalis Bb-12 и смешанных культур Lactococcus lactis ssp. с повышенными протеолитическими свойствами в составе замороженного бакконцентрата F DVS C-303 (или F DVS C-303) или в составе бакконцентрата FD DVS CH N-11 (или FD DVS CH N-19 или FD DVS CH N-22 или FD DVS Flora-danica), полученного лиофильной сушкой, позволяет получить продукты с гипоаллергенными свойствами. Самые высокие гипоаллергенные свойства имеют белковые пасты для детского питания, обогащенные лактулозой, комплексом витаминов FT 041081EU и комплексом минеральных веществ FT 042836EU: содержание α- и β-казеина в экспериментальных образцах продуктов, произведенных с использованием указанных заквасочных композиций, составляет 5,24…5,72 и 4,99...5,21 % соответственно, содержание комплекса κ-казеин+β-лактоглобулин – 1,13...1,42 %.У статті наведено результати визначення фракційного складу білків у пастах для дитячого харчування. Показано, що використання у технології білкових паст для дитячого харчування термостатного способу виробництва і розроблених заквашувальних композицій із монокультур B. animalis Bb-12 та змішаних культур Lactococcus lactis ssp. з підвищеними протеолітичними властивостями у складі замороженого бакконцентрату F DVS C-303 (або F DVS C-303) чи у складі бакконцентрату FD DVS CH N-11 (або FD DVS CH N-19, або FD DVS CH N-22, або FD DVS Flora-danica), отриманого ліофільним сушінням, дозволяє отримати продукти з гіпоалергенними властивостями. Найвищі гіпоалергенні властивості мають білкові пасти для дитячого харчування, збагачені лактулозою, комплексом вітамінів FT 041081EU та комплексом мінеральних речовин FT 042836EU: вміст  α- й β-казеїнів у експериментальних зразках продуктів, вироблених з використанням зазначених заквашувальних композицій, складає 5,24…5,72 й 4,99…5,21 % відповідно, вміст комплексу κ-казеїн+β-лактоглобулін – 1,13…1,42 %. &nbsp

How Well Does the Family Longevity Selection Score Work: A Validation Test Using the Utah Population Database

The Family Longevity Selection Score (FLoSS) was used to select families for the Long Life Family Study (LLFS) but has never been validated in other populations. The goal of this paper is to validate how well the FLoSS-based selection procedure works in an independent dataset. In this paper, we computed FLoSS using the lifespan data of 234,155 individuals from a large comprehensive genealogically-based resource, the Utah Population Database (UPDB), born between 1779 and 1910 with mortality follow-up through 2012–2013. Computations of FLoSS in a specific year (1980) confirmed the survival advantage of the “exceptional” sibships (defined by LLFS FLoSS threshold, FLoSS ≥ 7). We found that the subsample of the UPDB participants born after 1900 who were from the “exceptional” sibships had survival curves similar to that of the US participants from the LLFS probands' generation. Comparisons between the offspring of parents with “exceptional” and “ordinary” survival showed the survival advantage of the “exceptional” offspring. Investigators seeking to explain the extent genetics and environment contribute to exceptional survival will benefit from the use of exceptionally long-lived individuals and their relatives. Appropriate ranking of families by survival exceptionality and their availability for the purposes of providing genetic and phenotypic data is critical for selecting participants into such studies. This study validated the FLoSS as selection criteria in family longevity studies using UPDB

Sporadic Colorectal Cancer Development Shows Rejuvenescence Regarding Epithelial Proliferation and Apoptosis

Background and Aims: Sporadic colorectal cancer (CRC) development is a sequential process showing age-dependency, uncontrolled epithelial proliferation and decreased apoptosis. During juvenile growth cellular proliferation and apoptosis are well balanced, which may be perturbed upon aging. Our aim was to correlate proliferative and apoptotic activities in aging human colonic epithelium and colorectal cancer. We also tested the underlying molecular biology concerning the proliferation- and apoptosis-regulating gene expression alterations. Materials and Methods: Colorectal biopsies from healthy children (n1 = 14), healthy adults (n2 = 10), adult adenomas (n3 = 10) and CRCs (n4 = 10) in adults were tested for Ki-67 immunohistochemistry and TUNEL apoptosis assay. Mitosis- and apoptosis-related gene expression was also studied in healthy children (n1 = 6), adult (n2 = 41) samples and in CRC (n3 = 34) in HGU133plus2.0 microarray platform. Measured alterations were confirmed with RT-PCR both on dependent and independent sample sets (n1=6, n2=6, n3 = 6). Results: Mitotic index (MI) was significantly higher (p,0.05) in intact juvenile (MI = 0.3360.06) and CRC samples (MI = 0.4260.10) compared to healthy adult samples (MI = 0.1560.06). In contrast, apoptotic index (AI) was decreased in children (0.1360.06) and significantly lower in cancer (0.0660.03) compared to healthy adult samples (0.1760.05). Eight proliferation- (e.g. MKI67, CCNE1) and 11 apoptosis-associated genes (e.g. TNFSF10, IFI6) had altered mRNA expression both in the course of normal aging and carcinogenesis, mainly inducing proliferation and reducing apoptosis compared to healthy adults. Eight proliferation-associated genes including CCND1, CDK1, CDK6 and 26 apoptosis-regulating genes (e.g. SOCS3) were differently expressed between juvenile and cancer groups mostly supporting the pronounced cell growth in CRC. Conclusion: Colorectal samples from children and CRC patients can be characterized by similarly increased proliferative and decreased apoptotic activities compared to healthy colonic samples from adults. Therefore, cell kinetic alterations during colorectal cancer development show uncontrolled rejuvenescence as opposed to the controlled cell growth in juvenile colonic epithelium

An ancient bison from the mouth of the Rauchua River (Chukotka, Russia)

An incomplete carcass of an extinct bison, Bison ex gr. priscus, was discovered in 2012 in the mouth of the Rauchua River (69°30'N, 166°49'E), Chukotka. The carcass included the rump with two hind limbs, ribs, and large flap of hide from the abdomen and sides, several vertebrae, bones of the forelimbs and anterior autopodia, stomach with its contents, and wool. The limb bones are relatively gracile, which is unusual in bison, and a SEM study of the hair microstructure suggests higher insulating capacity than in extant members of the genus. Additionally, mitochondrial DNA analyses indicate that the Rauchua bison belonged to a distinct and previously unidentified lineage of steppe bison. Two radiocarbon dates suggest a Holocene age for the bison: a traditional 14C date provided an estimate of 8030±70 14C yr BP (SPb-743) and an AMS radiocarbon date provided an age of 9497±92 14C yr BP (AA101271). These dates make this the youngest known bison from Chukotka. Analysis of stomach contents revealed a diet of herbaceous plants (meadow grasses and sedges) and shrubs, suggesting that the early Holocene vegetation near the mouth of the Rauchua River was similar to that of the present day: tundra-associated vegetation with undersized plants