The use of Raman spectroscopy to identify and grade prostatic adenocarcinoma in vitro.

PublishedClinical TrialJournal ArticleThis is the final version of the article. Available from Cancer Research UK via the DOI in this record.Raman spectroscopy is an optical technique, which provides a measure of the molecular composition of tissue. Raman spectra were recorded in vitro from both benign and malignant prostate biopsies, and used to construct a diagnostic algorithm. The algorithm was able to correctly identify each pathological group studied with an overall accuracy of 89%. The technique shows promise as a method for objectively grading prostate cancer

Editorial

Editorial [sem resumo

Defining New Research Questions and Protocols in the Field of Traumatic Brain Injury through Public Engagement: Preliminary Results and Review of the Literature

Traumatic brain injury (TBI) is the most common cause of death and disability in the age group below 40 years. The financial cost of loss of earnings and medical care presents a massive burden to family, society, social care, and healthcare, the cost of which is estimated at £1 billion per annum (about brain injury (online)). At present, we still lack a full understanding on the pathophysiology of TBI, and biomarkers represent the next frontier of breakthrough discoveries. Unfortunately, many tenets limit their widespread adoption. Brain tissue sampling is the mainstay of diagnosis in neuro-oncology; following on this path, we hypothesise that information gleaned from neural tissue samples obtained in TBI patients upon hospital admission may correlate with outcome data in TBI patients, enabling an early, accurate, and more comprehensive pathological classification, with the intent of guiding treatment and future research. We proposed various methods of tissue sampling at opportunistic times: two methods rely on a dedicated sample being taken; the remainder relies on tissue that would otherwise be discarded. To gauge acceptance of this, and as per the guidelines set out by the National Research Ethics Service, we conducted a survey of TBI and non-TBI patients admitted to our Trauma ward and their families. 100 responses were collected between December 2017 and July 2018, incorporating two redesigns in response to patient feedback. 75.0% of respondents said that they would consent to a brain biopsy performed at the time of insertion of an intracranial pressure (ICP) bolt. 7.0% replied negatively and 18.0% did not know. 70.0% would consent to insertion of a jugular bulb catheter to obtain paired intracranial venous samples and peripheral samples for analysis of biomarkers. Over 94.0% would consent to neural tissue from ICP probes, external ventricular drains (EVD), and lumbar drains (LD) to be salvaged, and 95.0% would consent to intraoperative samples for further analysis

DESCENDENTES DESLOCADOS: UMA LEITURA DE O SOL SE PÕE EM SÃO PAULO E A CHAVE DE CASA

Neste trabalho, buscamos analisar como dois romances, O sol se põe em São Paulo, de Bernardo Carvalho (2007), e A chave de casa, de Tatiana Salem Levy (2008), problematizam a ideia de identidade nacional como algo rígido, único e fechado através do deslocamento de descendentes de imigrantes. No romance de Carvalho, no qual o leitor se depara com imigrantes e descendentes japoneses em uma história que se expande do Japão a São Paulo, percebe-se a busca por assimilação à nova nação e a demanda de êxito que recai sobre as novas gerações. O narrador, bisneto de imigrantes japoneses, relata sua aversão àquilo que pode o aproximar ao país de origem de seus antepassados assim como a importância de atingir um certo nível de sucesso para honrar os sacrifícios daqueles que imigraram. A obra de Levy também toca na questão da imigração, desta vez de pessoas oriundas da Turquia, e mostra não só a necessidade de assimilação como o romance de Carvalho, mas também a herança traumática que a imigração pode deixar para os descendentes. Na narrativa, o corpo doente da narradora-personagem, neta de imigrantes, serve como metáfora para essa herança. Uma leitura desses romances, portanto, possibilita uma problematização da visão de identidade nacional única brasileira

DESIGN, SUSTENTABILIDADE E ESPIRITUALIDADE: O RENASCIMENTO DE UM VÍNCULO

O artigo propõe um breve apanhado histórico sobre a relação entre design e espiritualidade, e como o desenvolvimento do eu interior é capaz de promover o real entendimento do conceito de sustentabilidade, relembrando que somos cocriadores e responsáveis pelo nosso mundo, pelo todo e pela nossa própria felicidade