Evaluación de parámetros de estrés oxidativo y actividades metabólicas de enfermeras trabajando en turnos diurnos y nocturnos

Se objetivó evaluar estrés oxidativo y actividades metabólicas de enfermeras en turnos diurnos y nocturnos. Participaron enfermeras de Unidad de Terapia Intensiva (UTI, n=70) y del servicio común (SC, n=70). Se obtuvieron muestras sanguíneas al inicio y al final de los turnos para medir parámetros de estrés oxidativo. Las actividades metabólicas también fueron analizadas utilizando brazaletes SenseWear. Los parámetros de estrés oxidativo aumentaron hacia el final de los turnos de todas las enfermeras SC y UTI, comparados con la medida de inicio. Los niveles de TAS, TOS y OSI no eran significativamente diferentes entre enfermeras SC y UTI al cierre de los turnos diurnos y nocturnos. Las actividades metabólicas de enfermeras SC y UTI se mostraron similares. Consecuentemente, los parámetros de estrés oxidativo y las actividades metabólicas de enfermeras SC y UTI no resultaron diferentes, y todas las enfermeras sufren efectos semejantes en sus turnos, tanto diurnos como nocturnos.O objetivo deste estudo foi avaliar o estresse oxidativo e as atividades metabólicas das enfermeiras em turnos diurnos e noturnos. Enfermeiras da Unidade de Tratamento Intensivo (UTI) (n=70) e do serviço comum (SC) (n=70) participaram do estudo. Logo no início e ao final dos turnos, amostras de sangue foram obtidas para medir parâmetros de estresse oxidativo. Atividades metabólicas também foram analisadas com o uso da braçadeira SenseWear. Parâmetros de estresse oxidativo aumentaram no fim dos turnos de todas as enfermeiras SC e UTI quando comparados ao início dos turnos. Comparados às enfermeiras SC, os níveis de TAS, TOS e OSI das enfermeiras de UTI não eram significativamente diferentes no final dos turnos diurnos e noturnos. Além disso, as atividades metabólicas das enfermeiras de SC e UTI se revelaram como sendo similares. Assim, os parâmetros de estresse oxidativo e as atividades metabólicas das enfermeiras SC e UTI não eram diferentes, e todas as enfermeiras sofrem efeitos semelhantes dos turnos, tanto no dia quanto na noite.The aim of this study was to evaluate the oxidative stress and metabolic activities of nurses working day and night shifts. Intensive care unit (ICU) (n=70) and ordinary service (OS) nurses (n=70) were enrolled in the study. Just before and the end of the shifts, blood samples were obtained to measure the participants' oxidative stress parameters. Metabolic activities were analyzed using the SenseWear Armband. Oxidative stress parameters were increased at the end of the shifts for all OS and ICU nurses compared to the beginning of the shifts. Compared to the OS nurses, the ICU nurses' TAS, TOS, and OSI levels were not significantly different at the end of the day and night shifts. The metabolic activities of the OS and ICU nurses were found to be similar. As a result, the OS and ICU nurses' oxidative stress parameters and metabolic activities were not different, and all of the nurses experienced similar effects from both the day and night shifts

Proanthocyanidin to prevent formation of the reexpansion pulmonary edema

<p>Abstract</p> <p>Background</p> <p>We aimed to investigate the preventive effect of Proanthocyanidine (PC) in the prevention of RPE formation.</p> <p>Methods</p> <p>Subjects were divided into four groups each containing 10 rats. In the Control Group (CG): RPE wasn't performed. Then subjects were followed up for three days and they were sacrificed after the follow up period. Samplings were made from tissues for measurement of biochemical and histopathologic parameters. In the Second Group (PCG): The same protocol as CG was applied, except the administration of PC to the subjects. In the third RPE Group (RPEG): Again the same protocol as CG was applied, but as a difference, RPE was performed. In the Treatment Group (TG): The same protocol as RPEG was applied except the administration of PC to the subjects.</p> <p>Results</p> <p>In RPEG group, the most important histopathological finding was severe pulmonary edema with alveolar damage and acute inflammatory cells. These findings were less in the TG group. RPE caused increased MDA levels, and decreased GPx, SOD and CAT activity significantly in lung tissue.</p> <p>Conclusion</p> <p>PC decreased MDA levels. Oxidative stress plays an important role in pathophysiology of RPE and PC treatment was shown to be useful to prevent formation of RPE.</p

Long non-coding RNA NKILA regulates expression of HSP90 alpha, NF-kappa B and beta-catenin proteins in the MCF-7 breast cancer cell line

Non-coding RNAs are increasingly being investigated and have shown great potential for diagnosis, prognosis and treatment of cancer. Thus, we have investigated a possible regulatory mechanism between NF-kappa B suppressor-NKILA, and HSP90, NF-kappa B, and beta-catenin molecules in MCF-7 breast cancer cells. HSP90 is an important stress protein and together with beta-catenin and NF-kappa B molecules can be responsible for cancer cell development. However, there is no comprehensive data available on the novel molecule NKILA unlike for HSP90, beta-catenin and NF-kappa B alone. Therefore, we suggest there might be a correlation between NKILA and these proteins. To investigate the NKILA role on HSP90, NF-kappa B and beta-catenin proteins we inhibited the NKILA by using transfection in MCF-7 breast cancer cells. NKILA-siRNA transfected cells were incubated for 5 h. Then, cells were collected and proteins were extracted to be separated by SDS-PAGE. The aforementioned proteins of siRNA transfected group were evaluated by quantification and comparison of their relative expression levels with the control group by immunoblotting. Results showed, HSP90 and NF-kappa B/p105, NF-kappa B/p65 and NF-kappa B/p50 subunits significantly increased while the level of beta-catenin decreased after NKILA inhibition. For the first time we have demonstrated that HSP90 and expression levels of beta-catenin are associated with NKILA levels which may be closely related to the canonical NF-kappa B pathway in MCF-7 cells. These novel findings may have significant implications in cancer cells development and possibly present important hints for the future studies of the cancer cell targeted therapy

Regional Differences in Equivalence Scales in Turkey

Equivalence scales have a crucial role in poverty measurement. For Turkey, there are no available up-todate equivalence scales, representing Turkish data. There were no efforts for calculation of equivalence scales at the regional level. The aim of the paper is to calculate and propose an equivalence scale for Turkey and estimate regional differences. Besides the models with Engel method, different equivalence scales were estimated by Almost Ideal Demand System. The results of the first model of AIDS approach composed of 5 age groups, is proposed for Turkey, but for simplicity the results of the second AIDS model could be used as well. In this model, the equivalence scale for Turkey is calculated as 0.65 for each additional adult after the first one and 0.35 for each child. For regional equivalence scales, we use two methods and comare the results. In the first one, regressions were run for each region separately and in the second one, dummy variables introduced. The highest difference in the results of the two methods was observed in Istanbul region. The findings for the regional scales are less reliable as the household size is bigger. This limitation is due to the relatively small size of the data sets. Having surveys with higher sample sizes would enable better results. After getting the results some conclusions could be drawn especially with regard to child cost differences among regions. It was expected to have higher costs for children in poorer regions and the regional results have confirmed this hypothesis

How to Reduce the Impact of Equivalence Scales on Poverty Measurement: Evidence from Turkey

The main aim of the paper is to contribute to the poverty measurement literature by demonstrating a method to reduce the impact of equivalence scales in poverty measurement. This is accomplished by choosing the most appropriate reference household type. The results showed that one adult household is certainly not suitable for being the reference household type. When one adult household is set as the reference, in the range of no equivalence scale and per capita equivalence scale, poverty head count ratio changes from 1 to 48 %, showing the huge effect of the equivalence scale choice. Also the analyses at household size level showed that one adult household type is not convenient to be the reference household type. On the other hand, no clear distinction could be made between central household types, but the importance of choosing a household type close to the center was demonstrated for Turkish data

Regional Differences in Equivalence Scales in Turkey

Equivalence scales have a crucial role in poverty measurement. For Turkey, there are no available up-todate equivalence scales, representing Turkish data. There were no efforts for calculation of equivalence scales at the regional level. The aim of the paper is to calculate and propose an equivalence scale for Turkey and estimate regional differences. Besides the models with Engel method, different equivalence scales were estimated by Almost Ideal Demand System. The results of the first model of AIDS approach composed of 5 age groups, is proposed for Turkey, but for simplicity the results of the second AIDS model could be used as well. In this model, the equivalence scale for Turkey is calculated as 0.65 for each additional adult after the first one and 0.35 for each child. For regional equivalence scales, we use two methods and comare the results. In the first one, regressions were run for each region separately and in the second one, dummy variables introduced. The highest difference in the results of the two methods was observed in Istanbul region. The findings for the regional scales are less reliable as the household size is bigger. This limitation is due to the relatively small size of the data sets. Having surveys with higher sample sizes would enable better results. After getting the results some conclusions could be drawn especially with regard to child cost differences among regions. It was expected to have higher costs for children in poorer regions and the regional results have confirmed this hypothesis

Anesthetic Management of a Patient with Motor-sensory polyneuropathy [Motor-Duyu Polinoropatili Olguda Anestezi Yonetimi]

Polyneuropathy that characterized by diffuse axonal degeneration or multifocal segmental demyelination is neuropathy and usually influence peripheral nerves. It can lead to respiratory failure with muscle weakness, atrophy, fasciculations, the involvement of the respiratory muscles. Increased risk of malignant hyperthermia, potential airway problems that may develop after extubation with effected the respiratory muscles, prolonged duration of recovery from non-depolarizing neuromuscular blockade, the discussion of the used neuraxial anesthesia makes anesthesia management important in patient with polyneuropathy. In this presentation aimed to provide general anesthesia management without neuromuscular blockade a patient with motor-sensory polyneuropathy mainly demyelinating who underwent posterior instrumentation due to scoliosis. [Med-Science 2016; 5(3.000): 884-8

The Effects of Positive End-Expiratory Pressure at Different Levels on Postoperative Respiration Parameters in Patients Undergoing Laparoscopic Cholecystectomy

Purpose/Aim of the study: We investigated the effects of different positive end-expiratory pressure (PEEP) levels on postoperative respiration parameters in patients undergoing laparoscopic cholecystectomy. Materials and Methods: One hundred and five consecutive patients were randomly divided to three groups (n = 35, for each group). Group I did not receive PEEP whereas group II received PEEP as 5 cmH2O and group III received PEEP as 8 cm H2O. Measurements with spirometer were taken 1 hour before the operation (T1) and, 1 (T2), 6 (T3), and 24 hours (T4) after extubation by an anesthetist. Forced expiratory volume (FEV1), forced vital capacity (FVC), and peak expiratory flow (PEF) were recorded. Results: Group I had higher FVC values measured at T2 time point compared with other groups (p < 0.05, for both groups). There was a statistically significant difference between group I and group III in terms of the FVC values measured at T3 and T4 time points (p = 0.05 and p < 0.05, retrospectively). A statistical difference was found between group I and group II in terms of FEV1 measured at T2 time point (p < 0.05). Group I had higher FEV1 values measured at T3 and T4 time points compared to group III (p = 0.05, P<0.05; respectively). Group III had lower PEF values measured at T4 time point compared to group I and II (p < 0.05). Conclusions: We concluded that PEEP with 5 or 8 cmH2O has negative effects on lung function tests measured with spirometer during postoperative 1st, 6th, and 24th hours in patients underwent elective laparoscopic cholecystectomy