26 research outputs found
Coupling of erbium-implanted silicon to a superconducting resonator
Erbium-implanted silicon is promising for both photonic and quantum-technology platforms, since it possesses both telecommunications and integrated-circuit processing compatibility. However, several different
Er
centers are generated during the implantation and annealing process, the presence of which could hinder the development of these applications. When
Si
is coimplanted with
10
17
cm
−
3
Er
and
10
20
cm
−
3
O
ions, and the appropriate annealing process is used, one of these centers, which is present at higher
Er
concentrations, can be eliminated. Characterization of samples with
Er
concentrations of <
10
17
cm
−
3
is limited by the sensitivity of standard electron paramagnetic resonance (EPR) instruments. The collective coupling strength between a superconducting (SC)
Nb
N
lumped-element resonator and a
10
17
cm
−
3
Er
-implanted
Si
sample at 20 mK is measured to be about 1 MHz, which provides a basis for the characterization of low-concentration
Er
-implanted
Si
and for future networks of hybrid quantum systems that exchange quantum information over the telecommunication network. Of six known
Er
-related EPR centers, only one trigonal center couples to the SC resonator
Biochemical, physiological, and performance response of a functional watermelon juice enriched in L-citrulline during a half-marathon race
Background: Watermelon is a rich natural source of l-citrulline. This non-essential amino acid increases exercise performance.
Objective: Evaluate the effect of Fashion watermelon juice enriched in l-citrulline (CWJ) (3.45 g per 500 mL) in physical performance and biochemical markers after a half-marathon race.
Design: A randomised, double blind, crossover design where 2 h after drinking 500 mL of CWJ or placebo (PLA, beverage without l-citrulline) amateur male runners performed two half-marathon races. Jump height, heart rate and rating of perceived exertion were evaluated before and after the races. Moreover, muscle soreness and plasma markers of muscle damage and metabolism were evaluated for 72 h after the races.
Results: Muscle soreness perception was significantly lower from 24 to 72 h after the race with CWJ beverage. Immediately after the races, runners under CWJ condition showed plasma lactate and glucose concentrations significantly lower and higher lactate dehydrogenase and l-arginine concentration than runners under PLA. A maintenance of jump heights after the races under CWJ supplementation was found, decreasing significantly with PLA.
Conclusion: A single Fashion watermelon juice enriched in l-citrulline dose diminished muscle soreness perception from 24 to 72 h after the race and maintained lower concentrations of plasma lactate after an exhausting exercise.Actividad Física y Deport
Influencing the properties of dysprosium single-molecule magnets with phosphine, phosphide and phosphinidene ligands
Single-molecule magnets are a type of coordination compound that can retain magnetic information at low temperatures. Single-molecule magnets based on lanthanides have accounted for many important advances, including systems with very large energy barriers to reversal of the magnetization, and a di-terbium complex that displays magnetic hysteresis up to 14 K and shows strong coercivity. Ligand design is crucial for the development of new single-molecule magnets: organometallic chemistry presents possibilities for using unconventional ligands, particularly those with soft donor groups. Here we report dysprosium single-molecule magnets with neutral and anionic phosphorus donor ligands, and show that their properties change dramatically when varying the ligand from phosphine to phosphide to phosphinidene. A phosphide-ligated, trimetallic dysprosium single-molecule magnet relaxes via the second-excited Kramers’ doublet, and, when doped into a diamagnetic matrix at the single-ion level, produces a large energy barrier of 256 cm1 and magnetic hysteresis up to 4.4 K
Chromosome studies of some Thinouia species (Sapindaceae) and the taxonomic implications
The karyotypes of three species of the genus Thinouia (Sapindaceae, Paullinieae) from southern Brazil and Bolivia are analyzed. New counts and karyotypes are reported for the first time for T ventricosa and T paraguayensis, both with 2n = 28. The karyotype formulae of T mucronata, T paraguayensis and T. ventricosa are 8m + 14sm + 6st, 10m + 8sm + 10st, and 14m + 4sm + 10st, respectively. In all the species investigated, the prophase chromosome condensation was always proximal and the pattern of the interphase nuclei was non-reticulate. These results are discussed in relation to previous chromosome studies for Paullinieae in order to infer the phylogenetic relationships within the tribe.o TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.451687
Differences and Resemblances in Banding Patterns and Ribosomal DNA Distribution in Four Species of Paullinieae Tribe (Sapindaceae)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)The tribe Paullinieae (Sapindaceae) is exclusively neotropically distributed, and is characterized by apomorphic characters and considered a monophyletic natural group. Recently explored cytogenetical aspects Suggest that the disploid chromosomal reduction, the increase in the chromosomal size and the diversification of highly repetitive DNA sequences are associated with the karyotypic evolution of this tribe. This work compares patterns of chromosome banding and the distribution of ribosomal DNA 18S-5.8S-26S in Cardiospermum grandiflorum Sw., Pullinia elegans Cambess., Urvillea chacoensis Hunz. and U ulmacea Kunth. The studied species share the presence of a pattern of terminal C-Giemsa bands, differentiated for characteristics of heterochromatic regions. Terminal AT-rich bands occurred in C. grandiflorum (2n=2x=20) and U. chacoensis (2n=2x=22). Differing from the others, U. chacoensis presented prominent bands in the majority of chromosomes. The polyploid cytotype of U. ulmacea (2n=8x=88) possessed terminal bands CMA(+) and DAPI(+), forming heterochromatic blocks constituted by GC- and AT-rich repetitive DNA. On the other hand, P elegans (2n=2x=24) presented a pattern of neutral bands after staining with CMA(3)/DAPI. The presence of GC-rich regions associated with 45S rDNA sites was a common characteristic in the studied species, nevertheless, variations in the NOR number might be useful for the differentiation of some species. Our results on karyological differences and resemblances of the studied species are discussed in relation to the systematics of the Paullinieae tribe.733283291Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP
Karyotype evolution and phylogenetic analyses in the genus Cardiospermum L. (Paullinieae, Sapindaceae)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Cardiospermum L. belongs to the Paullinieae tribe (Sapindaceae) and comprises 16 species. Of these, 12 species are present in South America and all occur in Brazil. Cardiospermum shows the most variable chromosome number of the tribe. Phylogenetic relationships within the genus Cardiospermum, especially with other species of the tribe, are poorly understood. This research focuses on characterisation of the karyotypic features of Cardiospermum using conventional cytogenetic methods, CMA/DAPI chromosome banding and fluorescence in situ hybridisation (FISH). To elucidate the phylogeny of the genus, the nuclear markers ITS1 and ITS2 were sequenced and analysed using maximum parsimony and Bayesian inference. Cardiospermum shows important diversity in basic numbers, with x = 7, 9, 10, 11 and 12. All species studied have metacentric and submetacentric chromosomes, some species have subtelocentric chromosomes, while telocentric chromosomes are absent. The interphase nuclei differentiate the Cardiospermum species into two groups. The CMA(3)/DAPI chromosome banding revealed the presence of an AT-rich terminal region in C. corindum, C. grandiflorum and C. urvilleoides, whereas GC-rich regions were found in C. grandiflorum, C. halicacabum var. halicacabum, C. halicacabum var. microcarpum, C. heringeri and C. integerrimum. FISH revealed syntenic and non-syntenic distribution of the 18-5.8-26S and 5S rDNA. The syntenic distribution always occurred in the short arms of the same chromosome in all of the species. The phylogenetic relationships reveal, in part, the taxonomic arrangement of the genus Cardiospermum.155868881Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)CONICETANPCyT-UNNE of ArgentinaConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP
Karyotypes, heterochromatin, and physical mapping of 18S-26S rDNA in Cactaceae
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Karyotype analyses in members of the four Cactaceae subfamilies were performed. Numbers and karyotype formula obtained were: Pereskioideae = Pereskia aculeata (2n = 22; 10 m + 1 sm), Maihuenioideae = Maihuenia patagonica (2n = 22, 9 m + 2 sm; 2n = 44, 18 m + 4 sm), Opuntioideae = Cumulopuntia recurvata (2n = 44; 20 m + 2 sm), Cactoideae = Acanthocalycium spiniflorum (2n = 22; 10 m + 1 sm), Echinopsis tubiflora (2n = 22; 10 m + 1 sm), Trichocereus candicans (2n = 22, 22 m). Chromosomes were small, the average chromosome length was 2.3 mu m. Diploid species and the tetraploid C. recurvata had one terminal satellite, whereas the remaining tetraploid species showed four satellited chromosomes. Karyotypes were symmetrical. No CMA(-)/DAPI(+) bands were detected, but CMA(+)/DAPI(-) bands associated with NOR were always found. Pericentromeric heterochromatin was found in C. recurvata, A. spiniflorum, and the tetraploid cytotype of M. patagonica. The locations of the 18S-26S rDNA sites in all species coincided with CMA(+)/DAPI(-) bands; the same occurred with the sizes and numbers of signals for each species. This technique was applied for the first time in metaphase chromosomes in cacti. NOR-bearing pair no.1 may be homeologous in all species examined. In Cactaceae, the 18S-26S loci seem to be highly conserved. Copyright (C) 2009 S. Karger AG, Basel12417280'Consejo Nacional de Investigaciones Cientificas y Tecnicas' (CONICET, Argentina)FONCYT'Universidad Nacional de Cordoba' (SECyT, Argentina)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
Molecular and cytogenetic characterization of an AT-rich satellite DNA family in Urvillea chacoensis Hunz. (Paullinieae, Sapindaceae)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Urvillea chacoensis is a climber with 2n = 22 and some terminal AT-rich heterochromatin blocks that differentiate it from other species of the genus. The AT-rich highly repeated satellite DNA was isolated from U. chacoensis by the digestion of total nuclear DNA with HindIII and XbaI and cloned in Escherichia coli. Satellite DNA structure and chromosomal distribution were investigated. DNA sequencing revealed that the repeat length of satDNA ranges between 721 and 728 bp, the percentage of AT-base pairs was about 72-73% and the studied clones showed an identity of 92.5-95.9%. Although this monomer has a tetranucleosomal size, direct imperfect repetitions of similar to 180 bp subdividing it in four nucleosomal subregions were observed. The results obtained with FISH indicate that this monomer usually appears distributed in the terminal regions of most chromosomes and is associated to heterochromatin blocks observed after DAPI staining. These observations are discussed in relation to the satellite DNA evolution and compared with other features observed in several plant groups.1361171177Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP