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Expression regulation of MAO isoforms in monocytic cells in response to Th2 cytokines
Background: Th2-cytokines, such as interleukins-4 and –13 (IL-4, IL-13), have been identified as alternative stimuli of monocytes/macrophages. We have recently profiled the gene-expression pattern of IL-4-teated human peripheral monocytes and found that 15-lipoxygenase-1 (15-LOX1) and monoamine oxidase A (MAO-A) are among the five most strongly upregulated gene products in IL-4-treated cells. Transfection of monocytic cells (U937) with 15-LOX1 also induced MAO-A expression. These data suggested that 15-LOX1 products might play a role in the IL4-induced signaling cascade leading to expression of MAO-A in human monocytes. Material/Methods: To test this hypothesis we incubated wild-type and 15-LOX1-transfected U937 cells with different concentrations of either IL-4 or 15-LOX-products [13S-H(p)ODE, 15S-H(p)ETE] and quantified the expression of 15-LOX1, MAO-A, and MAO-B by activity assays and real-time RT-PCR. Results: Wild-type U937 cells express neither MAO-A nor MAO-B, but after three days of IL4 treatment, MAO-A mRNA was detected. A similar isoform-specific expression of MAO-A mRNA was observed when U937 cells were transfected with 15-LOX1 or when the cells were incubated with primary 15-LOX1 products (hydroperoxy fatty acids) or H2O2. In contrast, the corresponding hydroxy fatty acids were ineffective. Conclusions: These data indicate that increased intracellular peroxide concentrations (oxidative stress) induce MAO-A expression in monocytes/macrophages, which normally do not express the enzyme. Our findings also suggest that IL-4-induced upregulation of MAO-A expression in human peripheral monocytes may proceed via 15-LOX1-dependent and 15-LOX1-independent pathways. The biological role of MAO-A expression for monocyte function is discussed
The effect of flooding on the exchange of the volatile Câ‚‚-compounds ethanol, acetaldehyde and acetic acid between leaves of Amazonian floodplain tree species and the atmosphere
The effect of root inundation on the leaf emissions of ethanol, acetaldehyde and acetic acid in relation to assimilation and transpiration was investigated with 2–3 years old tree seedlings of four Amazonian floodplain species by applying dynamic cuvette systems under greenhouse conditions. Emissions were monitored over a period of several days of inundation using a combination of Proton Transfer Reaction Mass Spectrometry (PTR-MS) and conventional techniques (HPLC, ion chromatography). Under non-flooded conditions, none of the species exhibited measurable emissions of any of the compounds, but rather low deposition of acetaldehyde and acetic acid was observed instead. Tree species specific variations in deposition velocities were largely due to variations in stomatal conductance. Flooding of the roots resulted in leaf emissions of ethanol and acetaldehyde by all species, while emissions of acetic acid were only observed from the species exhibiting the highest ethanol and acetaldehyde emission rates. All three compounds showed a similar diurnal emission profile, each displaying an emission burst in the morning, followed by a decline in the evening. This concurrent behavior supports the conclusion, that all three compounds emitted by the leaves are derived from ethanol produced in the roots by alcoholic fermentation, transported to the leaves with the transpiration stream and finally partly converted to acetaldehyde and acetic acid by enzymatic processes. Co-emissions and peaking in the early morning suggest that root ethanol, after transportation with the transpiration stream to the leaves and enzymatic oxidation to acetaldehyde and acetate, is the metabolic precursor for all compounds emitted, though we can not totally exclude other production pathways. Emission rates substantially varied among tree species, with maxima differing by up to two orders of magnitude (25–1700 nmol m−2 min−1 for ethanol and 5–500 nmol m−2 min−1 for acetaldehyde). Acetic acid emissions reached 12 nmol m−2 min−1. The observed differences in emission rates between the tree species are discussed with respect to their root adaptive strategies to tolerate long term flooding, providing an indirect line of evidence that the root ethanol production is a major factor determining the foliar emissions. Species which develop morphological root structures allowing for enhanced root aeration produced less ethanol and showed much lower emissions compared to species which lack gas transporting systems, and respond to flooding with substantially enhanced fermentation rates and a non-trivial loss of carbon to the atmosphere. The pronounced differences in the relative emissions of ethanol to acetaldehyde and acetic acid between the tree species indicate that not only the ethanol production in the roots but also the metabolic conversion in the leaf is an important factor determining the release of these compounds to the atmosphere
Health, education, and retirement over the prolonged life cycle: a selective survey of recent research
Fear of campaign violence and support for democracy and autocracy
Election violence is common in many developing countries and has potentially detrimental implications for democratic consolidation. Drawing on political psychology, we argue that citizens’ fear of campaign violence undermines support for democracy while increasing support for autocracy. Using individual-level survey data from 21 electoral democracies in Sub-Saharan Africa, we find robust support for our argument. Citizens fearing campaign violence are less likely to support democracy and multi-party competition, more likely to favor a return to autocracy, and less likely to turn out to vote. Our findings have important implications for democratic survival and provide further impetus for reducing electoral violence
Electroweak Sudakov Logarithms and Real Gauge-Boson Radiation in the TeV Region
Electroweak radiative corrections give rise to large negative,
double-logarithmically enhanced corrections in the TeV region. These are partly
compensated by real radiation and, moreover, affected by selecting
isospin-noninvariant external states. We investigate the impact of real gauge
boson radiation more quantitatively by considering different restricted final
state configurations. We consider successively a massive abelian gauge theory,
a spontaneously broken SU(2) theory and the electroweak Standard Model. We find
that details of the choice of the phase space cuts, in particular whether a
fraction of collinear and soft radiation is included, have a strong impact on
the relative amount of real and virtual corrections.Comment: 20 pages, 4 figure
Reducing decoherence of the confined exciton state in a quantum dot by pulse-sequence control
We study the phonon-induced dephasing of the exciton state in a quantum dot
excited by a sequence of ultra-short pulses. We show that the multiple-pulse
control leads to a considerable improvement of the coherence of the optically
excited state. For a fixed control time window, the optimized pulsed control
often leads to a higher degree of coherence than the control by a smooth single
Gaussian pulse. The reduction of dephasing is considerable already for 2-3
pulses.Comment: Final version (moderate changes
Measuring Global Similarity between Texts
We propose a new similarity measure between texts which, contrary to the
current state-of-the-art approaches, takes a global view of the texts to be
compared. We have implemented a tool to compute our textual distance and
conducted experiments on several corpuses of texts. The experiments show that
our methods can reliably identify different global types of texts.Comment: Submitted to SLSP 201
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