13 research outputs found
Selection of Trichoderma spp. strains for the control of Sclerotinia sclerotiorum in soybean
Preliminary characterization of bacteria from genus Pseudomonas causing soft rot of onion (Allium cepa L.) in Poland
Poszukiwanie źródeł odporności na choroby w odmianach dawnych i miejscowych oraz dzikich gatunkach pokrewnych zbóż. Praca przeglądowa
Waste materials as growing media for antagonistic Trichoderma fungi
Konsekwencją unowocześniania polskiego rolnictwa i specjalizacji gospodarstw jest coraz większy problem z bezpiecznym dla środowiska naturalnego wykorzystaniem różnego rodzaju odpadów organicznych, powstających w trakcie produkcji i przetwarzania produktów rolnych. Celem badań było określenie tempa wzrostu i zarodnikowania wybranych izolatów antagonistycznych grzybów Trichoderma na podłożach organicznych, skomponowanych z materiałów odpadowych. W doświadczeniach wykorzystano gatunki: Trichoderma harzianum izolaty TRS 61, TRS 59, TRS 69 oraz T. atroviride izolaty TRS 14 i TRS 6. Komponentami służącymi do wytwarzania podłoży organicznych były materiały odpadowe z gospodarstw rolnych oraz odpady z produkcji przetworów warzywnych i gospodarstwa domowego. Stwierdzono, że analizowane izolaty grzybów Trichoderma najlepiej rosły i zarodnikowały na podłożach zawierających w swoim składzie słomę pszenną moczoną w wodach drenażowych z upraw pod osłonami, odpad z marchwi, ziemniaka lub buraka oraz na podłożach zawierających podłoże popieczarkowe z dodatkiem odpadu z marchwi i buraka. Wyniki uzyskane w tej pracy wskazują, że różnorodne organiczne odpady można wykorzystać w rolnictwie jako nośniki mikroorganizmów antagonistycznych.A growing problem with safe for the environment utilization of a variety of organic waste, arising during the production and processing of agricultural products, is a consequence of modernization and specialization of Polish agriculture. Objective of the research was to examine the growth and sporulation of selected isolates of Trichoderma fungi on organic media, composed from a variety of waste materials. In experiments the isolates of Trichoderma harzianum isolates TRS 61, TRS 59, TRS 69 and T. atroviride TRS 14, TRS 6 were used. Components used for the production of organic substrates were waste materials from farms, from the vegetable-processing industry and household appliances. It was found that the analyzed fungal isolates of Trichoderma best grew and sporulated on the media containing wheat straw soaked in waste nutrient solution (WD) obtained from soilless culture system with the addition of carrot, potato or beetroot waste and on media containing substrate after cultivated mushrooms with carrot and beetroot waste. The results obtained in this work show that it is possible the use of a variety of organic wastes as carriers of antagonistic microorganisms, that could be used to grow plants
Prevalence of ESBL-producing Pseudomonas aeruginosa isolates in Warsaw, Poland, detected by various phenotypic and genotypic methods
Extract of Mimosa tenuiflora and Quercus robur as potential eco-friendly management tool against Sclerotinia sclerotiorum in Lactuca sativa enhancing the natural plant defences
Irritable bowel syndrome and functional dyspepsia in patients with eating disorders ‐ a systematic review
Objectives: The prevalence of eating disorders is rising worldwide. The low body weight in anorexia nervosa as well as the increase in body mass index due to binge eating disorder are contributing to a strikingly high morbidity and mortality. In a similar pattern, the prevalence and burden of the disease of functional gastrointestinal disorders such as functional dyspepsia and irritable bowel syndrome is increasing. As gastrointestinal complaints are commonly reported by patients with eating disorders, the question arose whether there is a relationship between eating disorders and functional gastrointestinal disorders.
Methods: To address the need to better understand the interplay between eating disorders and functional gastrointestinal disorders as well as factors that might influence this connection, the data bases Medline, Web of Science and Embase were systematically searched.
Results: After removal of duplicates the search yielded 388 studies which were screened manually. As a result, 36 publications were selected for inclusion in this systematic review.
Conclusion: The occurrence of functional gastrointestinal disorders like irritable bowel syndrome and functional dyspepsia in patients with eating disorders is considerably high and often associated with psychological, hormonal and functional alterations. In the future, further research addressing the underlying mechanisms accounting for this relationship is required
Analysis of the impact of sex and age on the variation in the prevalence of antinuclear autoantibodies in Polish population:a nationwide observational, cross-sectional study
The detection of antinuclear autoantibody (ANA) is dependent on many factors and varies between the populations. The aim of the study was first to assess the prevalence of ANA in the Polish adult population depending on age, sex and the cutoff threshold used for the results obtained. Second, we estimated the occurrence of individual types of ANA-staining patterns. We tested 1731 patient samples using commercially available IIFA using two cutoff thresholds of 1:100 and 1:160. We found ANA in 260 participants (15.0%), but the percentage of positive results strongly depended on the cutoff level. For a cutoff threshold 1:100, the positive population was 19.5% and for the 1:160 cutoff threshold, it was 11.7%. The most prevalent ANA-staining pattern was AC-2 Dense Fine speckled (50%), followed by AC-21 Reticular/AMA (14.38%) ANA more common in women (72%); 64% of ANA-positive patients were over 50 years of age. ANA prevalence in the Polish population is at a level observed in other highly developed countries and is more prevalent in women and elderly individuals. To reduce the number of positive results released, we suggest that Polish laboratories should set 1:160 as the cutoff threshold.</p
Relationship Between Anti-DFS70 Autoantibodies and Oxidative Stress
Background:
The anti-DFS70 autoantibodies are one of the most commonly and widely described agent of unknown clinical significance, frequently detected in healthy individuals. It is not known whether the DFS70 autoantibodies are protective or pathogenic. One of the factors suspected of inducing the formation of anti-DFS70 antibodies is increased oxidative stress. We evaluated the coexistence of anti-DFS70 antibodies with selected markers of oxidative stress and investigated whether these antibodies could be considered as indirect markers of oxidative stress.
Methods:
The intensity of oxidative stress was measured in all samples via indices of free-radical damage to lipids and proteins such as total oxidant status (TOS), concentrations of lipid hydroperoxides (LPH), lipofuscin (LPS), and malondialdehyde (MDA). The parameters of the non-enzymatic antioxidant system, such as total antioxidant status (TAS) and uric acid concentration (UA), were also measured, as well as the activity of superoxide dismutase (SOD). Based on TOS and TAS values, the oxidative stress index (OSI) was calculated. All samples were also tested with indirect immunofluorescence assay (IFA) and 357 samples were selected for direct monospecific anti DFS70 enzyme-linked immunosorbent assay (ELISA) testing. Results:: The anti-DFS70 antibodies were confirmed by ELISA test in 21.29% of samples. Compared with anti-DFS70 negative samples we observed 23% lower concentration of LPH (P = .038) and 11% lower concentration of UA (P = .005). TOS was 20% lower (P = .014). The activity of SOD was up to 5% higher (P = .037). The Pearson correlation showed weak negative correlation for LPH, UA, and TOS and a weak positive correlation for SOD activity.
Conclusion:
In samples positive for the anti-DFS70 antibody a decreased level of oxidative stress was observed, especially in the case of samples with a high antibody titer. Anti-DFS70 antibodies can be considered as an indirect marker of reduced oxidative stress or a marker indicating the recent intensification of antioxidant processes