Study of CAPE effect on apoptosis induction in AGS human gastric cancer cell line

Background: Propolis is a natural product of bee and caffeic acid phenethyl ester (CAPE) is a pharmacologically important product of propolis. Objectives: The aim of this study was to investigate the effect of CAPE on apoptosis induction in AGS human gastric cancer cells. Materials and Methods: The cytotoxic effects of CAPE at different concentrations were investigated on AGS cells viability after 24 hours treatment by MTT assay. To measure the effect of CAPE on apoptosis induction, AGS cells were treated with CAPE for 24 hours and investigated by FITC Annexin V/PI staining using flow cytometry. Results: CAPE prevented growth and proliferation of AGS human gastric cancer cell line in a concentration-dependent manner with an IC50 of approximately 60 μM by a 24-hour treatment. Also CAPE caused increased induction of apoptosis in AGS cells from 1.37 % in control cells to 21.76 % in treated cells with 30 μM CAPE. Conclusions: CAPE prevents growth and proliferation of AGS human gastric cancer cell line through inducing programmed cell death in AGS cells. Therefore, CAPE could be helpful for developing chemotherapeutic agents or as an adjuvant for human gastric cancer treatment. © 2016, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences

THE EFFECT OF STEROID TREATMENT ON CORROSIVE ESOPHAGEAL BURNS IN CHILDREN

WOS: A1991HA54500003PubMed ID: 1777488A retrospective study was done on corrosive oesophageal burns to ascertain the preventive effect of corticosteroids on stricture development. Within the last 12 years 351 children were admitted to our medical centre with a history of corrosive agent ingestion. In 235 of these the diagnosis of oesophageal burn was confirmed by means of oesophagoscopy. Children admitted within the first 48 hours received steroid, antibiotic and fluid therapy while fluid and antibiotics were given, if needed, in the rest. Forty-six of the children were lost to follow-up. The type of corrosive agent, the admission period, the degree of the burn and the stricture development were used as parameters. Stricture development was found statistically significant in late admitted patients vs. early admissions. This retrospective study suggests the effectiveness of corticosteroid treatment in preventing the stricture development

in a premature neonate

Endotracheal intubation is a common daily procedure used for sustaining life in premature and term neonates in the neonatal intensive care unit. This procedure can be associated with some iatrogenic complications. One of these complications is ingesting a laryngoscope light bulb, and it is an extremely rare complication in neonates. The consequences of ingestion or aspiration may be serious and life-threatening. Herein, a male premature neonate, ingested a laryngoscope light bulb during endotracheal intubation because of surfactant replacement therapy, is reported. This case re-affirms the importance of checking the integrity of equipment before intubation effort

in a premature neonate

Endotracheal intubation is a common daily procedure used for sustaining life in premature and term neonates in the neonatal intensive care unit. This procedure can be associated with some iatrogenic complications. One of these complications is ingesting a laryngoscope light bulb, and it is an extremely rare complication in neonates. The consequences of ingestion or aspiration may be serious and life-threatening. Herein, a male premature neonate, ingested a laryngoscope light bulb during endotracheal intubation because of surfactant replacement therapy, is reported. This case re-affirms the importance of checking the integrity of equipment before intubation effort

A different aspect of corrosive ingestion in children: socio-demographic characteristics and effect of family functioning.

OBJECTIVE: Corrosive ingestion is thoroughly investigated in terms of its complications and treatment modalities but to date family functioning, behavior, attitude and knowledge of mothers of children who ingested corrosives has not been investigated and socio-demographic data has rarely been mentioned. Therefore a study was planned to investigate the socio-demographic data, family functioning, knowledge, behavior and attitude of mothers of children who ingested corrosive materials. METHODS: The mothers of 50 children who ingested corrosive materials and 60 controls were asked to answer a questionnaire which included socio-demographic data and questions about their attitude, behavior and knowledge of corrosive ingestion. The mothers were also asked to reply family assessment device (FAD) and parental attitude research instrument (PARI). RESULTS: The average ages of the children were 5.31+/-2.32 in the corrosive group with an M:F ratio of 1:1. The level of education of both mothers (p=0.000) and fathers (p=0.000) in the corrosive group were lower than that of the controls. There was no difference between the two groups in terms of number of working mothers (p=0.085). In the corrosive group the families used to have three or more children (42%) and the socioeconomic status of this group was lower than the controls (p=0.001). In the corrosive group these substances were purchased unlabeled (64%) and kept mainly in coke bottles. The affective involvement dimension of the FAD and the attitude of over-parenting and democratic attitude dimensions of the PARI tests were significantly different in the corrosive group among mothers from lower educational level whereas no difference was detected among mothers from higher educational level in this regard. CONCLUSION: It was found that both level of education of the parents and socioeconomic factors played important role in shaping the habits that might lead to corrosive ingestion in children. For the prevention of corrosive ingestion broad based strategies including education are required

Congenital Cystic Adenomatoid Malformation: Case Report

Congenital cystic adenomatoid malformation (CCAM) is characterized by disorganized, hamartomatous and adenomatoid proliferation of primary bronchiole and occurs with the frequency of 1/11000-1/35000. Histopathologically, there are three types of CCAM, differing in the number and size of the cysts. Usually, surgical management for the symptomatic cases and clinical follow up for the asymptomatic cases are recommended. In this article, a 35-week-old premature infant with CCAM presenting with respiratory distress on postnatal fifth day was reported. A mass covering the 2/3 of the right lung and pushing the mediastinum to the left was determined by the chest radiography and the thorax computerized tomography. When the infant was 12 days old, right upper lobectomy and right lower lobe posterobasal segmentectomy were performed. Histopathologic examination was consistent with CCAM type I. The postoperative period was uneventful. On account of this patient, the importance of early surgical treatment for the symptomatic cases with CCAM was emphasized

Konjenital kistik adenomatoid malformasyonlu bir olgu sunumu

Congenital cystic adenomatoid malformation (CCAM) is characterized by disorganized, hamartomatous and adenomatoid proliferation of primary bronchiole and occurs with the frequency of 1/11000-1/35000. Histopathologically, there are three types of CCAM, differing in the number and size of the cysts. Usually, surgical management for the symptomatic cases and clinical follow up for the asymptomatic cases are recommended. In this article, a 35-week-old premature infant with CCAM presenting with respiratory distress on postnatal fifth day was reported. A mass covering the 2/3 of the right lung and pushing the mediastinum to the left was determined by the chest radiography and the thorax computerized tomography. When the infant was 12 days old, right upper lobectomy and right lower lobe posterobasal segmentectomy were performed. Histopathologic examination was consistent with CCAM type I. The postoperative period was uneventful. On account of this patient, the importance of early surgical treatment for the symptomatic cases with CCAM was emphasized. © 2010 by Türkiye Klinikleri