New viruses found in fig exhibiting mosaic symptoms

Mosaic is the most widespread viral disease of fig, affecting the crop wherever it is grown. The causal agent of the disease was poorly characterized and until recently it was considered a virus-like agent with double membrane bound semispherical bodies transmitted by eriophyid mites. During the molecular characterization of the Fig mosaic virus we discovered two new closteroviruses and a new badnavirus affecting the tree used in our studies. The characterization and presence of the three new viruses in mosaic-affected plants is the subject of this communication. Keywords: Fig mosaic, Emaravirus, Closterovirus, Badnaviru

Making silicon hydrophobic: wettability control by two-lengthscale simultaneous patterning with femtosecond laser irradiation

We report on the wettability properties of silicon surfaces, simultaneously structured on the micrometre-scale and the nanometre-scale by femtosecond (fs) laser irradiation to render silicon hydrophobic. By varying the laser fluence, it was possible to control the wetting properties of a silicon surface through a systematic and reproducible variation of the surface roughness. In particular, the silicon–water contact angle could be increased from 66° to more than 130°. Such behaviour is described by incomplete liquid penetration within the silicon features, still leaving partially trapped air inside. We also show how controllable design and tailoring of the surface microstructures by wettability gradients can drive the motion of the drop's centre of mass towards a desired direction (even upwards)

Blueberry latent virus in highbush blueberry (Vaccinium corymbosum L.) in Bosnia and Herzegovina

A small-scale survey for blueberry viruses in Bosnia and Herzegovina was performed in 2018. A total of 20 samples from three locations were collected and analyzed for the presence of 11 viruses. ELISA assays were performed for blueberry scorch virus, blueberry shock virus, blueberry shoestring virus, blueberry leaf mottle virus, tobacco ringspot virus, and tomato ring spot virus. The samples were tested for blueberry red ringspot virus by PCR, and blueberry fruit drop associated virus, blueberry latent virus, blueberry mosaic associated virus, blueberry necrotic ring blotch virus, blueberry virus A, and blueberry leaf mottle virus by RT-PCR. The analyses confirmed the presence of BlLV in eight samples with no other virus detected in any of the samples

Natural occurrence of Cucumber mosaic virus infecting water mint (Mentha aquatica) in Antalya and Konya, Turkey

A virus causing a disease in mint (the aromatic and culinary plant) has recently become a problem in the Taurus Mountains, a mountain range in the Mediterranean region of Turkey. To detect the virus and investigate its distribution in the region, mint leaf samples were collected from the vicinity of spring areas in the plateaus of Antalya and Konya in 2009. It was found that Cucumber mosaic virus (CMV) was detected in 27.08% of symptomatic samples tested by DAS-ELISA. To the best of our knowledge, this is the first report of CMV on mint plants in this region of Turkey

Informedia at TRECVID 2003: Analyzing and searching broadcast news video

We submitted a number of semantic classifiers, most of which were merely trained on keyframes. We also experimented with runs of classifiers were trained exclusively on text data and relative time within the video, while a few were trained using all available multiple modalities. 1.2 Interactive search This year, we submitted two runs using different versions of the Informedia systems. In one run, a version identical to last year's interactive system was used by five researchers, who split up the topics between themselves. The system interface emphasizes text queries, allowing search across ASR, closed captions and OCR text. The result set can then be manipulated through: • storyboards of images spanning across video story segments • emphasizing matching shots to a user’s query to reduce the image count to a manageable size • resolution and layout under user control • additional filtering provided through shot classifiers such as outdoors, and shots with people, etc. • display of filter count and distribution to guide their use in manipulating storyboard views. In the best-performing interactive run, for all topics a single researcher used an improved version of the system, which allowed more effective browsing and visualization of the results of text queries using

A computational study on outliers in world music

The comparative analysis of world music cultures has been the focus of several ethnomusicological studies in the last century. With the advances of Music Information Retrieval and the increased accessibility of sound archives, large-scale analysis of world music with computational tools is today feasible. We investigate music similarity in a corpus of 8200 recordings of folk and traditional music from 137 countries around the world. In particular, we aim to identify music recordings that are most distinct compared to the rest of our corpus. We refer to these recordings as ‘outliers’. We use signal processing tools to extract music information from audio recordings, data mining to quantify similarity and detect outliers, and spatial statistics to account for geographical correlation. Our findings suggest that Botswana is the country with the most distinct recordings in the corpus and China is the country with the most distinct recordings when considering spatial correlation. Our analysis includes a comparison of musical attributes and styles that contribute to the ‘uniqueness’ of the music of each country

Fetal lung underdevelopment is rescued by administration of amniotic fluid stem cell extracellular vesicles in rodents

Fetal lung underdevelopment, also known as pulmonary hypoplasia, is characterized by decreased lung growth and maturation. The most common birth defect found in babies with pulmonary hypoplasia is congenital diaphragmatic hernia (CDH). Despite research and clinical advances, babies with CDH still have high morbidity and mortality rates, which are directly related to the severity of lung underdevelopment. To date, there is no effective treatment that promotes fetal lung growth and maturation. Here, we describe a stem cell–based approach in rodents that enhances fetal lung development via the administration of extracellular vesicles (EVs) derived from amniotic fluid stem cells (AFSCs). Using fetal rodent models of pulmonary hypoplasia (primary epithelial cells, organoids, explants, and in vivo), we demonstrated that AFSC-EV administration promoted branching morphogenesis and alveolarization, rescued tissue homeostasis, and stimulated epithelial cell and fibroblast differentiation. We confirmed this regenerative ability in in vitro models of lung injury using human material, where human AFSC-EVs obtained following good manufacturing practices restored pulmonary epithelial homeostasis. Investigating EV mechanism of action, we found that AFSC-EV beneficial effects were exerted via the release of RNA cargo. MicroRNAs regulating the expression of genes involved in lung development, such as the miR17–92 cluster and its paralogs, were highly enriched in AFSC-EVs and were increased in AFSC-EV–treated primary lung epithelial cells compared to untreated cells. Our findings suggest that AFSC-EVs hold regenerative ability for underdeveloped fetal lungs, demonstrating potential for therapeutic application in patients with pulmonary hypoplasia

ICTV virus taxonomy profile: Ophioviridae

The Ophioviridae is a family of filamentous plant viruses, with single-stranded negative, and possibly ambisense, RNA genomes of 11.3-12.5 kb divided into 3-4 segments, each encapsidated separately. Virions are naked filamentous nucleocapsids, forming kinked circles of at least two different contour lengths. The sole genus, Ophiovirus, includes seven species. Four ophioviruses are soil-transmitted and their natural hosts include trees, shrubs, vegetables and bulbous or corm-forming ornamentals, both monocots and dicots. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Ophioviridae, which is available at http://www.ictv.global/report/ophioviridae.Instituto de Biotecnologia y Biologia MolecularFacultad de Ciencias Agrarias y Forestale