6,049 research outputs found
On the maximum bias functions of MM-estimates and constrained M-estimates of regression
We derive the maximum bias functions of the MM-estimates and the constrained
M-estimates or CM-estimates of regression and compare them to the maximum bias
functions of the S-estimates and the -estimates of regression. In these
comparisons, the CM-estimates tend to exhibit the most favorable
bias-robustness properties. Also, under the Gaussian model, it is shown how one
can construct a CM-estimate which has a smaller maximum bias function than a
given S-estimate, that is, the resulting CM-estimate dominates the S-estimate
in terms of maxbias and, at the same time, is considerably more efficient.Comment: Published at http://dx.doi.org/10.1214/009053606000000975 in the
Annals of Statistics (http://www.imstat.org/aos/) by the Institute of
Mathematical Statistics (http://www.imstat.org
Profilin reduces aggregation and phase separation of huntingtin N-terminal fragments by preferentially binding to soluble monomers and oligomers
Shotgun ion mobility mass spectrometry sequencing of heparan sulfate saccharides
Despite evident regulatory roles of heparan sulfate (HS) saccharides in numerous biological processes, definitive information on the bioactive sequences of these polymers is lacking, with only a handful of natural structures sequenced to date. Here, we develop a “Shotgun” Ion Mobility Mass Spectrometry Sequencing (SIMMS2) method in which intact HS saccharides are dissociated in an ion mobility mass spectrometer and collision cross section values of fragments measured. Matching of data for intact and fragment ions against known values for 36 fully defined HS saccharide structures (from di- to decasaccharides) permits unambiguous sequence determination of validated standards and unknown natural saccharides, notably including variants with 3O-sulfate groups. SIMMS2 analysis of two fibroblast growth factor-inhibiting hexasaccharides identified from a HS oligosaccharide library screen demonstrates that the approach allows elucidation of structure-activity relationships. SIMMS2 thus overcomes the bottleneck for decoding the informational content of functional HS motifs which is crucial for their future biomedical exploitation
Molecular Line Observations of the Small Protostellar Group L1251B
We present molecular line observations of L1251B, a small group of pre- and
protostellar objects, and its immediate environment in the dense C18O core
L1251E. These data are complementary to near-infrared, submillimeter and
millimeter continuum observations reported by Lee et al. (2006, ApJ, 648, 491;
Paper I). The single-dish data of L1251B described here show very complex
kinematics including infall, rotation and outflow motions, and the
interferometer data reveal these in greater detail. Interferometer data of N2H+
1-0 suggest a very rapidly rotating flattened envelope between two young
stellar objects, IRS1 and IRS2. Also, interferometer data of CO 2-1 resolve the
outflow associated with L1251B seen in single-dish maps into a few narrow and
compact components. Furthermore, the high resolution data support recent
theoretical studies of molecular depletions and enhancements that accompany the
formation of protostars within dense cores. Beyond L1251B, single-dish data are
also presented of a dense core located ~150" to the east that, in Paper I, was
detected at 850 micron but has no associated point sources at near- and
mid-infrared wavelengths. The relative brightness between molecules, which have
different chemical timescales, suggests it is less chemically evolved than
L1251B. This core may be a site for future star formation, however, since line
profiles of HCO+, CS, and HCN show asymmetry with a stronger blue peak, which
is interpreted as an infall signature.Comment: 46 pages, 18 figures. Accepted for publication in Ap
SCUBA Mapping of Spitzer c2d Small Clouds and Cores
We present submillimeter observations of dark clouds that are part of the
Spitzer Legacy Program, From Molecular Cores to Planet-Forming Disks (c2d). We
used the Submillimetre Common User's Bolometer Array to map the regions
observed by Spitzer by the c2d program to create a census of dense molecular
cores including data from the infrared to the submillimeter. In this paper, we
present the basic data from these observations: maps, fluxes, and source
attributes. We also show data for an object just outside the Perseus cloud that
was serendipitously observed in our program. We propose that this object is a
newly discovered, evolved protostar.Comment: 37 pages, accepted to The Astronomical Journa
Inhibition of Cellular Protein Secretion by Norwalk Virus Nonstructural Protein p22 Requires a Mimic of an Endoplasmic Reticulum Export Signal
Protein trafficking between the endoplasmic reticulum (ER) and Golgi apparatus is central to cellular homeostasis. ER export signals are utilized by a subset of proteins to rapidly exit the ER by direct uptake into COPII vesicles for transport to the Golgi. Norwalk virus nonstructural protein p22 contains a YXΦESDG motif that mimics a di-acidic ER export signal in both sequence and function. However, unlike normal ER export signals, the ER export signal mimic of p22 is necessary for apparent inhibition of normal COPII vesicle trafficking, which leads to Golgi disassembly and antagonism of Golgi-dependent cellular protein secretion. This is the first reported function for p22. Disassembly of the Golgi apparatus was also observed in cells replicating Norwalk virus, which may contribute to pathogenesis by interfering with cellular processes that are dependent on an intact secretory pathway. These results indicate that the ER export signal mimic is critical to the antagonistic function of p22, shown herein to be a novel antagonist of ER/Golgi trafficking. This unique and well-conserved human norovirus motif is therefore an appealing target for antiviral drug development
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