Morphology of the rat’s brain in four vessels model of ischemic stroke after administration of carbamylated darbepoetin

The aim was the study of the cerebroprotective effect of carbamylated darbepoetin in a four-vessel model of rat brain ischemia-reperfusion. A massive preconditioning cerebroprotective effect of carbamylated darbepoetin in the fourth group of animals and massive ischemic changes of the hippocampal and cortical neurons in the third group were revealed. These were manifested by karyopyknosis, karyorrhexis, and violation of stratification of CA1-CA3 layers of the hippocampu

Quinoidal Azaacenes: 99 % Diradical Character

Quinoidal azaacenes with almost pure diradical character (y=0.95 to y=0.99) were synthesized. All compounds exhibit paramagnetic behavior investigated by EPR and NMR spectroscopy, and SQUID measurements, revealing thermally populated triplet states with an extremely low-energy gap ΔEST′ of 0.58 to 1.0 kcal mol−1. The species are persistent in solution (half-life≈14–21 h) and in the solid state they are stable for weeks

Deoxygenation affects composition of membrane-bound proteins in human erythrocytes

Background/Aims: ATP release from erythrocyte plays a key role in hypoxia-induced elevation of blood flow in systematic circulation. We have previously shown that hemolysis contributes to erythrocyte ATP release triggered by several stimuli, including hypoxia, but the molecular mechanisms of hypoxia-increased membrane fragility remain unknown. Methods: In this study, we compared the action of hypoxia on hemolysis, ATP release and the composition of membrane-bound proteins in human erythrocytes. Results: Twenty minutes incubation of human erythrocytes in the oxygen-free environment increased the content of extracellular hemoglobin by ∼1.5 fold. Paired measurements of hemoglobin and ATP content in the same samples, showed a positive correlation between hemolysis and ATP release. Comparative analysis of SDS-PAGE electrophoresis of erythrocyte ghosts obtained under control and deoxygenated conditions revealed a ∼2-fold elevation of the content of membrane-bound protein with Mr of ∼60 kDa. Conclusion: Deoxygenation of human erythrocytes affects composition of membrane-bound proteins. Additional experiments should be performed to identify the molecular origin of 60 kDa protein and its role in the attenuation of erythrocyte integrity and ATP release in hypoxic conditions

Исследование матричной полимеризации анилина в присутствии смесей полимерных сульфокислот и свойств образующихся интерполимерных комплексов

The polymerization of aniline in the presence of mixtures of polymer sulfonic acid <http://lingvo.yandex.ru/en?text=sulfonic acid&st_translate=on&lang_from=en&lang_to=ru&dict=Medical>s has been studied. It was shown dominating influence of rigid polyacid on polymerization kinetics and electronic structure of formed polyaniline.Изучена полимеризация анилина в присутствии смесей полисульфокислот, различающихся гибкостью цепи макромолекулы. Показано доминирующее влияние жесткоцепной поликислоты на кинетику полимеризации и электронную структуру образующегося полианилина

Матричный синтез полианилина в присутствии полимерной сульфокислоты

It was investigated the influence of the correlation of sulfoacid and aniline concentration on the polymerization of the latter when it was protonated by polymeric sulfoacid and on electronic structure of forming polyaniline.Исследовано влияние соотношения концентраций сульфокислотных групп и анилина на полимеризацию последнего при его протонировании по- лимерной сульфокислотой и электрон- ную структуру образующегося полиани- лина

Study of matrix polymerization of polyaniline in the presence of mixtures of polymeric sulfonic acids and properties of formed interpolymer complexes

The polymerization of aniline in the presence of mixtures of polymer sulfonic acid <http://lingvo.yandex.ru/en?text=sulfonic acid&st_translate=on&lang_from=en&lang_to=ru&dict=Medical>s has been studied. It was shown dominating influence of rigid polyacid on polymerization kinetics and electronic structure of formed polyaniline

Template synthesis of polyaniline in presence of polymeric sulfoacid

It was investigated the influence of the correlation of sulfoacid and aniline concentration on the polymerization of the latter when it was protonated by polymeric sulfoacid and on electronic structure of forming polyaniline

ADDITIVE NEUROPROTECTIVE EFFECT OF 3-HYDROXYPYRIDINE DERIVATIVES AND HUMAN ERYTHROPOETIN ANALOGUE ON A HEMORRHAGIC STROKE MODEL IN RATS

The correction of free radical oxidation processes is one of the most promising strategies of neuroprotection in acute cerebrovascular disorders.The aim of the study is an experimental study of the neuroprotective effects of 3-hydroxypyridine and erythropoietin derivatives, as well as their combined use.Materials and methods. The study was performed on 109 male Wistar rats. The neuroprotective effect of the substances was studied on a hemorrhagic stroke model. The study drugs were administered to the animals intraperitoneally. Carbamylated darbepoetin was administered three times in advance at the dose of 100 µg/kg within intervals of 3 days, the last injection took place 1 hour before the operation (the total dose was 300 mg/kg). Etoxidol was administered once 1 hour before the surgery at the dose of 50 mg/kg. The survival rate, behavioral features and the state of the animals on the 1st, 3rd, 7th and 14th days were recorded, and the morphological assessment of the brain was carried out.Results. The investigated substances had a positive effect on both the survival rate of the animals during the first day and on the 14th day. The best survival rates on the 14th day were recorded in the group of a combined use of ethoxydol and carbamylated darbepoetin (75%). Thus, in this group of rats, a faster recovery of neurological disorders was already distinguished from the first day on. By the 7th day, more than 50% of the rats receiving the combination of the studied drugs, had had a slight neurological deficit (up to 3 points on the McGrow scale); by the 14th day there had been only minor changes in the neurological status in the rats of this group. A pronounced neuroprotective effect of the combination of 3-hydroxypyridine and erythropoietin derivatives has been confirmed by a histological examination of brain slices – a more rapid decrease in the size of perifocal edema and microcirculation disorders, less damage to neurons and glial elements, and faster processes of resorption and organization of hemorrhage. A macroscopic examination of the brain sections stained with triphenyltetrazolium chloride of the dying rats, showed that perifocal necrosis had been the main cause of high mortality in the control group after the 3rd day.Conclusion. As a result of the experiment, the nephroprotective effect of the studied derivatives of 3-hydroxypyridine and erythropoietin has been proved. Moreover, the combination of these drugs has shown a greater neuroprotective activity than their isolated use. The additive effect of these drugs was due to their action mechanism resulting from the synergism of various structures and components of the cells