Archeogeophysical Studies in The Sultan Ahmet (Blue) Mosque

Bu çalışmada İstanbul Fatih ilçesinde bulunan Sultan Ahmet Camii’nin içinde, dış avlularda, tüm alt bahçe alanlarında ve dış parsellerde gerçekleştirilen yeraltı haritalama çalışmaları anlatılmaktadır. Mayıs-Temmuz 2011 tarihleri arasında gerçekleştirilen arazi ölçümleri ve saha çalışmaları sonucunda Sultan Ahmet Camii oturum sahasının yaklaşık 30 metre derinliğe kadar olan kısımların yeraltı haritaları oluşturulmuştur. Bu sayede daha önce varlığından emin olunamayan çok sayıda yapay ve doğal yer içi oluşumu haritalanması ve (3B) yeriçi modellerinin oluşturulması amaçlanmıştır

Tracking the embryonic stem cell transition from ground state pluripotency

Mouse embryonic stem (ES) cells are locked into self-renewal by shielding from inductive cues. Release from this ground state in minimal conditions offers a system for delineating developmental progression from naive pluripotency. Here we examined the initial transition process. The ES cell population behaves asynchronously. We therefore exploited a short-half-life $\textit{Rex1::GFP}$ reporter to isolate cells either side of exit from naive status. Extinction of ES cell identity in single cells is acute. It occurs only after near-complete elimination of naïve pluripotency factors, but precedes appearance of lineage specification markers. Cells newly departed from the ES cell state display features of early post-implantation epiblast and are distinct from primed epiblast. They also exhibit a genome-wide increase in DNA methylation, intermediate between early and late epiblast. These findings are consistent with the proposition that naive cells transition to a distinct formative phase of pluripotency preparatory to lineage priming.This research was funded by the Wellcome Trust (091484/Z/10/Z and 095645/Z/11/Z), the Biotechnology and Biological Sciences Research Council (BB/M004023/1 and BB/K010867/1), a European Commission Framework 7 project EuroSyStem (HEALTH-F4-2007-200720 EUROSYSTEM), SysStemCell (ERC-2013-AdG 339431), the Medical Research Council (MRC) (G1100526/1) the Louis-Jeantet Foundation and the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO-VIDI 864.12.007). The Cambridge Stem Cell Institute receives core funding from the Wellcome Trust and Medical Research Council (MRC). A.S. is an MRC Professor. Deposited in PMC for immediate release

Prevalence of leishmaniosis in dogs in Istanbul, Turkey determined by using PCR

Leishmania infantum is widespread in Mediterranean countries including Turkey and can cause a serious disease in both humans and dogs. Dogs are considered to be its main reservoirs. The current epidemiological study was carried out in Istanbul for detection of leishmaniosis among dogs. A total of 246 dogs were included in the study. Twenty one (8.54%) blood samples were found to be positive based on PCR diagnosis, using primers specific for the kinetoplast DNA of Leishmania. Infection rate was 6.51% in 169 dogs living in shelters and 12.99% in 77 client-owned dogs. The rate was significantly (p<0.001) higher in the dogs (37.93%) presenting one or more clinical symptoms which may be attributable to leishmaniosis, than in the asymptomatic dogs (4.61%). Eleven (52.4%) of 21 PCR positive dogs presented clinical symptoms whereas the rest of the dogs (47.62%) were asymptomatic. The major clinical sign in PCR positive animals was dermatological problems. Amongst PCR positive dogs, skin lesions were present in 11, weight loss in 2, lymphadenopathy in 2, epistaxis in 2 and lethargy in 2 dogs