Rilevamento delle sorgenti doppler della ionosfera tramite radar-HF

Il presente lavoro contiene un insieme di concetti di base utili per comprendere la cosiddetta tecnica della “interferometria doppler”, usata, nell’ambito degli studi sulla ionosfera, per ricavare informazioni sulla conformazione e velocità degli strati riflettenti (operazione chiamata anche sky mapping). È utile ricordare, infatti, che il sondaggio ionosferico tradizionale consente solo la determinazione dell’altezza virtuale di ogni strato, pensato come un unico oggetto riflettente piano. Tale determinazione è giunta nel tempo ad avere una risoluzione molto spinta, dell’ordine di qualche chilometro, tuttavia una ionosonda tradizionale non possiede la capacità di individuare la struttura degli strati riflettenti

In Silico Design, Synthesis and Biological Evaluation of Anticancer Arylsulfonamide Endowed with Anti-Telomerase Activity

Telomerase, a reverse transcriptase enzyme involved in DNA synthesis, has a tangible role in tumor progression. Several studies have evidenced telomerase as a promising target for developing cancer therapeutics. The main reason is due to the overexpression of telomerase in cancer cells (85–90%) compared with normal cells where it is almost unexpressed. In this paper, we used a structure-based approach to design potential inhibitors of the telomerase active site. The MYSHAPE (Molecular dYnamics SHared PharmacophorE) approach and docking were used to screen an in-house library of 126 arylsulfonamide derivatives. Promising compounds were synthesized using classical and green methods. Compound 2C revealed an interesting IC50 (33 ± 4 µM) against the K-562 cell line compared with the known telomerase inhibitor BIBR1532 IC50 (208 ± 11 µM) with an SI ~10 compared to the BALB/3-T3 cell line. A 100 ns MD simulation of 2C in the telomerase active site evidenced Phe494 as the key residue as well as in BIBR1532. Each moiety of compound 2C was involved in key interactions with some residues of the active site: Arg557, Ile550, and Gly553. Compound 2C, as an arylsulfonamide derivative, is an interesting hit compound that deserves further investigation in terms of optimization of its structure to obtain more active telomerase inhibitors

Rilevamento delle sorgenti doppler della ionosfera tramite radar-HF

Il presente lavoro contiene un insieme di concetti di base utili per comprendere la cosiddetta tecnica della “interferometria doppler”, usata, nell’ambito degli studi sulla ionosfera, per ricavare informazioni sulla conformazione e velocità degli strati riflettenti (operazione chiamata anche sky mapping). È utile ricordare, infatti, che il sondaggio ionosferico tradizionale consente solo la determinazione dell’altezza virtuale di ogni strato, pensato come un unico oggetto riflettente piano. Tale determinazione è giunta nel tempo ad avere una risoluzione molto spinta, dell’ordine di qualche chilometro, tuttavia una ionosonda tradizionale non possiede la capacità di individuare la struttura degli strati riflettenti

The new AIS-INGV digital ionosonde

A new digital ionosonde called AIS-INGV (Advanced Ionospheric Sounder) was designed both for research and for routine service of HF radio wave propagation forecast. Nearly the entire system was developed in the Laboratorio di Geofisica Ambientale at the Istituto Nazionale di Geofisica e Vulcanologia (INGV), Rome. It exploits advanced techniques for signal analysis, recent technological devices and PC resources. This paper describes design concepts and performance of the new ionosonde

THE NEW INGV DIGITAL IONOSONDE: DESIGN REPORT

The ionosonde is a system which exploits the radar technique: it applies electromagnetic waves with variable frequency in the HF band to measure the ionospheric layers electron density, height and other parameters. This paper is a technical report on the new digital ionosonde (AIS-INGV), which was designed both for research purposes and for the routine service of the HF radiowave propagation forecast. It has been developed almost completely within the Laboratorio di Geofisica Ambientale (LGA) at the Istituto Nazionale di Geofisica e Vulcanologia (INGV). It exploits advanced techniques for the signal analysis, recent technological devices and PC resources. The report is divided into two parts; the first is a general description of the design development, the second is a more detailed description of the blocks and circuits actually built and tested, directed to a specialist reader

Evaluation of the IKKβ Binding of Indicaxanthin by Induced-Fit Docking, Binding Pose Metadynamics, and Molecular Dynamics

Background: Indicaxanthin, a betaxanthin belonging to the betalain class of compounds, has been recently demonstrated to exert significant antiproliferative effects inducing apoptosis of human melanoma cells through the inhibition of NF-κB as the predominant pathway. Specifically, Indicaxanthin inhibited IκBα degradation in A375 cells. In resting cells, NF-κB is arrested in the cytoplasm by binding to its inhibitor protein IκBα. Upon stimulation, IκBα is phosphorylated by the IKK complex, and degraded by the proteasome, liberating free NF-κB into the nucleus to initiate target gene transcription. Inhibition of the IKK complex leads to the arrest of the NF-κB pathway. Methods: To acquire details at the molecular level of Indicaxanthin’s inhibitory activity against hIKKβ, molecular modeling and simulation techniques including induced-fit docking (IFD), binding pose metadynamics (BPMD), molecular dynamics simulations, and MM-GBSA (molecular mechanics-generalized Born surface area continuum solvation) have been performed. Results: The computational calculations performed on the active and inactive form, and the allosteric binding site of hIKKβ, revealed that Indicaxanthin inhibits prevalently the active form of the hIKKβ. MM-GBSA computations provide further evidence of Indicaxanthin’s stability inside the active binding pocket with a binding free energy of −22.2 ± 4.3 kcal/mol with respect to the inactive binding pocket with a binding free energy of −20.7 ± 4.7 kcal/mol. BPMD and MD simulation revealed that Indicaxanthin is likely not an allosteric inhibitor of hIKKβ. Conclusion: As a whole, these in silico pieces of evidence show that Indicaxanthin can inhibit the active form of the hIKKβ adding novel mechanistic insights on its recently discovered ability to impair NF-κB signaling in melanoma A375 cells. Moreover, our results suggest the phytochemical as a new lead compound for novel, more potent IKKβ inhibitors to be employed in the treatment of cancer and inflammation-related conditions

IONOSONDA A COMPRESSIONE DI IMPULSI - NOTA TECNICA PRELIMINARE

Nel laboratorio di geofisica ambientale dell'Istituto Nazionale di Geofisica e Vulcanologia è stato progettato e realizzato il prototipo di una ionosonda a bassa potenza a compressione di impulsi secondo i dettami della moderna tecnica radaristica. Si è puntato sulla realizzazione di una macchina a bassa potenza e dal costo contenuto con possibilità di costituire una rete di ionosonde per scopi di sorveglianza ionosferica. Il sistema con una potenza di 200W consente di investigare la ionosfera da 90 a 700km con una risoluzione verticale di 5km in un range di frequenza da 1 a 20MHz

Ionospheric Observatory Development At Mario Zucchelli Station

Since 1995 Italian Ionospheric Antarctic Observatory at Terra Nova Bay, now “MARIO ZUCCHELLI”, station (geographic coordinates: 74.70°S, 164.11°E) performs continuous and systematic ionospheric vertical soundings. Long time series of continuous and accurate ionospheric observations (more than one solar cycle) are necessary for a deeper understanding of the complex phenomena occurring in the upper atmosphere at high latitude; furthermore high rate soundings (at least four soundings per hour or more) contribute to the short-time prediction of the radiopropagation conditions and to the Space Weather. During 2003–2004 Antarctic campaign a new digital ionosonde, recently developed at the Istituto Nazionale di Geofisica e Vulcanologia (INGV) in Rome, (Italy), has been installed the Ionospheric Observatory and preliminary tests have been carried out. This new Advanced Ionospheric Sounder-INGV, briefly AIS, is integrated in a stand alone system during winter time: the sounding, device settings and data sending to Rome are completely automatic and remote programmable. Ionograms are available on line at the INGV web and ftp server. The new features of the Ionospheric Observatory are presented and preliminary statistics on the reliability and validation of the experimental observation are shown and discussed

Preliminary design of a RESPER probe prototype, configured in a multi dipole-dipole array.

The RESPER probe has been assembled, except the metallic points that must ensure the contact, by insulating materials and more specifically in Tufnol, as regards the support plates, and Teflon, as regards the standoffs. A series of holes has been drilled on the surface of support plates in order to allow approaching each other of the two central electrodes to external ones, from a minimum of 4.29 cm to a maximum of 10 cm, as shown in the table of configurations. The dipole-dipole array defined by the integer parameter n = 6 could not be implemented, as the positioning of suitable “spring” poles requires 6 mm holes and an adequate space could not be available to carry out the drilling. The presence of these “spring” poles allows reaching a right prominence of the tip from the base and, at the same time, a certain amount of pressure which ensures the proper adherence to the artifact that must be tested. There is a brass screw within each pole, which edge has been turned to the measurement of 1.4 mm. Four metal spacers are replaced of time in time depending on the configuration that is to be adopted. The achievement of height l(n) from the plates is ensured by the presence of metallic spacers, which dimensions are reported. To complete the description, a copper cable, 1 m long and with a 4.0 mm2 section area, has been welded to the head of each electrode. The two transmitting or reading cables are kept at a fixed distance L(n) between them by means of Teflon rods in which has been applied the same series of holes existing on the Tufnol plates