Diversity and Community Structure of Stream Insects in a Minimally Disturbed Forested Watershed in Southern Illinois

The Lusk Creek Watershed, located in Pope County, IL, long has been rec- ognized as a high quality area of biological significance, but surveys of the stream macroinvertebrate fauna have been limited. Thus, a survey of the benthic insect community at 11 sites in the upper portion of Lusk Creek was conducted from May 2003 to April 2005. A total of 20,888 specimens, mostly immatures, were examined during the study and represented eight orders. The Diptera, by far, was the most abundant order, with 18,590 specimens, almost all of which were members of the Chironomidae or Simuliidae. Members of the EPT (Ephemer- optera, Plecoptera, Trichoptera) contributed 1,550 specimens. The Coleoptera was represented by 647 specimens, most of which were members of Stenelmis (Elmidae) (n = 612). The Shannon diversity index (H ́) ranged from 1.07-2.01 for individual sites and was indicative of relatively undisturbed streams in this region. Jackknife analyses of richness estimated that as many as 37 taxa were unobserved in this survey. Results provide information on reference conditions in the region and a foundation for future monitoring

Establishment and operation of a Good Manufacturing Practice-compliant allogeneic Epstein-Barr virus (EBV)-specific cytotoxic cell bank for the treatment of EBV-associated lymphoproliferative disease

Funded by Wellcome Trust Translational Award SNBTSPeer reviewedPublisher PD

Properties and biases of the global heat flow compilation

Geothermal heat flow is inferred from the gradient of temperature values in boreholes or short-penetration probe measurements. Such measurements are expensive and logistically challenging in remote locations and, therefore, often targeted to regions of economic interest. As a result, measurements are not distributed evenly. Some tectonic, geologic and even topographic settings are overrepresented in global heat flow compilations; other settings are underrepresented or completely missing. These limitations in representation have implications for empirical heat flow models that use catalogue data to assign heat flow by the similarity of observables. In this contribution, we analyse the sampling bias in the Global Heat Flow database of the International Heat Flow Commission; the most recent and extensive heat flow catalogue, and discuss the implications for accurate prediction and global appraisals. We also suggest correction weights to reduce the bias when the catalogue is used for empirical modelling. From comparison with auxiliary variables, we find that each of the following settings is highly overrepresented for heat flow measurements; continental crust, sedimentary rocks, volcanic rocks, and Phanerozoic regions with hydrocarbon exploration. Oceanic crust, cratons, and metamorphic rocks are underrepresented. The findings also suggest a general tendency to measure heat flow in areas where the values are elevated; however, this conclusion depends on which auxiliary variable is under consideration to determine the settings. We anticipate that using our correction weights to balance disproportional representation will improve empirical heat flow models for remote regions and assist in the ongoing assessment of the Global Heat Flow database

Performing heritage: the use of live 'actors' in heritage presentations

This paper investigates the phenomenon of 'living history' presentations of heritage, using live 'actors' to portray historical characters. Its aim is to discuss these presentations in the context of what may be understood as 'heritage', and of the nature of 'performance'. Four case studies of heritage sites, each important as a tourist attraction, have been selected for detailed study, together with a number of other examples of heritage performance. It is clear from the empirical work that different performance strategies are employed within the heritage industry and by individual 'actors'. Most of the performers take part as a leisure activity, and many do not consider themselves to be 'performing' at all. The greatest concern of participants lies in the degree of authenticity of the performance. Through 'living history', the 'actors' are drawn into an experience of heritage which has real meaning for them, and which may contribute both to a sense of identity and to an enhanced understanding of society, past and present. The popularity of such presentations with visitors also indicates that similar benefits are perceived by the 'audience'

Diagnostic accuracy of circulating-free DNA for the determination of MYCN amplification status in advanced-stage neuroblastoma: a systematic review and meta-analysis

Abstract: Background: MYCN amplification (MNA) is the strongest indicator of poor prognosis in neuroblastoma (NB). This meta-analysis aims to determine the diagnostic accuracy of MNA analysis in circulating-free DNA (cfDNA) from advanced-stage NB patients. Methods: A systematic review of electronic databases was conducted to identify studies exploring the detection of MNA in plasma/serum cfDNA from NB patients at diagnosis using PCR methodology. Pooled estimates for sensitivity, specificity and diagnostic odds ratio (DOR) were calculated by conducting a bivariate/HSROC random-effects meta-analysis. Results: Seven studies, with a total of 529 advanced-stage patients, were eligible. The pooled sensitivity of cfDNA-based MNA analysis was 0.908 (95% CI, 0.818–0.956), the pooled specificity was 0.976 (0.940–0.991) and the DOR was 410.0 (−103.6 to 923.7). Sub-grouped by INSS stage, the sensitivity for stage 3 and 4 patients was 0.832 (0.677–0.921) and 0.930 (0.834–0.972), respectively. The specificity was 0.999 (0.109–1.000) and 0.974 (0.937–0.990), respectively, and the DOR was 7855.2 (−66267.0 to 81977.4) and 508.7 (−85.8 to 1103.2), respectively. Conclusions: MNA analysis in cfDNA using PCR methodology represents a non-invasive approach to rapidly and accurately determine MNA status in patients with advanced-stage NB. Standardised methodology must be developed before this diagnostic test can enter the clinic

Deletion of airway cilia results in noninflammatory bronchiectasis and hyperreactive airways

The mechanisms for the development of bronchiectasis and airway hyperreactivity have not been fully elucidated. Although genetic, acquired diseases and environmental influences may play a role, it is also possible that motile cilia can influence this disease process. We hypothesized that deletion of a key intraflagellar transport molecule, IFT88, in mature mice causes loss of cilia, resulting in airway remodeling. Airway cilia were deleted by knockout of IFT88, and airway remodeling and pulmonary function were evaluated. In IFT88− mice there was a substantial loss of airway cilia on respiratory epithelium. Three months after the deletion of cilia, there was clear evidence for bronchial remodeling that was not associated with inflammation or apparent defects in mucus clearance. There was evidence for airway epithelial cell hypertrophy and hyperplasia. IFT88− mice exhibited increased airway reactivity to a methacholine challenge and decreased ciliary beat frequency in the few remaining cells that possessed cilia. With deletion of respiratory cilia there was a marked increase in the number of club cells as seen by scanning electron microscopy. We suggest that airway remodeling may be exacerbated by the presence of club cells, since these cells are involved in airway repair. Club cells may be prevented from differentiating into respiratory epithelial cells because of a lack of IFT88 protein that is necessary to form a single nonmotile cilium. This monocilium is a prerequisite for these progenitor cells to transition into respiratory epithelial cells. In conclusion, motile cilia may play an important role in controlling airway structure and function

Safety profile of autologous macrophage therapy for liver cirrhosis

This work was supported by a Medical Research Council UK grant (Biomedical Catalyst Major Awards Committee; reference MR/M007588/1) to S.J. Forbes. We thank Z.M. Younossi (Center for Outcomes Research in Liver Diseases, Washington, DC, USA) for academic use of the CLDQ instrument and L.J. Fallowfield (Sussex Health Outcomes Research & Education in Cancer (SHORE-C), University of Sussex, UK) for advice about health-related quality of life assessment.Peer reviewedPostprintPostprintPostprintPostprin