Motor units as tools to evaluate profile of human Renshaw inhibition

Although Renshaw inhibition (RI) has been extensively studied for decades, its precise role in motor control is yet to be discovered. One of the main handicaps is a lack of reliable methods for studying RI in conscious human subjects. We stimulated the lowest electrical threshold motor axons (thickest axons) in the tibial nerve and analysed the stimulus‐correlated changes in discharge of voluntarily recruited low‐threshold single motor units (SMUs) from the soleus muscle. In total, 54 distinct SMUs from 12 subjects were analysed. Stimuli that generated only the direct motor response (M‐only) on surface electromyography induced an inhibitory response in the low‐threshold SMUs. Because the properties of RI had to be estimated indirectly using the background discharge rate of SMUs, its profile varied with the discharge rate of the SMU. The duration of RI was found to be inversely proportional to the discharge rate of SMUs. Using this important finding, we have developed a method of extrapolation for estimating RI as it develops on motoneurons in the spinal cord. The frequency methods indicated that the duration of RI was between 30 and 40 ms depending on the background firing rate of the units, and the extrapolation indicated that RI on silent motoneurons was ∼55 ms. The present study establishes a novel methodology for studying RI in human subjects and hence may serve as a tool for improving our understanding of the involvement of RI in human motor control

Grating coupler integrated photodiodes for plasmon resonance based sensing

Cataloged from PDF version of article.In this work, we demonstrate an integrated sensor combining a grating-coupled plasmon resonance surface with a planar photodiode. Plasmon enhanced transmission is employed as a sensitive refractive index (RI) sensing mechanism. Enhanced transmission of light is monitored via the integrated photodiode by tuning the angle of incidence of a collimated beam near the sharp plasmon resonance condition. Slight changes of the effective refractive index (RI) shift the resonance angle, resulting in a change in the photocurrent. Owing to the planar sensing mechanism, the design permits a high areal density of sensing spots. In the design, absence of holes that facilitate resonant transmission of light, allows an easy-to-implement fabrication procedure and relative insensitivity to fabrication errors. Theoretical and experimental results agree well. An equivalent long-term RI noise of 6.3 x 10(-6) RIU/root Hz is obtained by using an 8 mW He-Ne laser, compared to a shot-noise limited theoretical sensitivity of 5.61 x 10(-9) RIU/root Hz. The device features full benefits of grating-coupled plasmon resonance, such as enhancement of sensitivity for non-zero azimuthal angle of incidence. Further sensitivity enhancement using balanced detection and optimal plasmon coupling conditions are discussed

Short-term plasticity of neuro-auditory processing induced by musical active listening training

Although there is strong evidence for the positive effects of musical training on auditory perception, processing, and training-induced neuroplasticity, there is still little knowledge on the auditory and neurophysiological short-term plasticity through listening training. In a sample of 37 adolescents (20 musicians and 17 nonmusicians) that was compared to a control group matched for age, gender, and musical experience, we conducted a 2-week active listening training (AULOS: Active IndividUalized Listening OptimizationS). Using magnetoencephalography and psychoacoustic tests, the short-term plasticity of auditory evoked fields and auditory skills were examined in a pre-post design, adapted to the individual neuro-auditory profiles. We found bilateral, but more pronounced plastic changes in the right auditory cortex. Moreover, we observed synchronization of the auditory evoked P1, N1, and P2 responses and threefold larger amplitudes of the late P2 response, similar to the reported effects of musical long-term training. Auditory skills and thresholds benefited largely from the AULOS training. Remarkably, after training, the mean thresholds improved by 12 dB for bone conduction and by 3–4 dB for air conduction. Thus, our findings indicate a strong positive influence of active listening training on neural auditory processing and perception in adolescence, when the auditory system is still developing

Measuring vertebrate telomeres: applications and limitations

Telomeres are short tandem repeated sequences of DNA found at the ends of eukaryotic chromosomes that function in stabilizing chromosomal end integrity. In vivo studies of somatic tissue of mammals and birds have shown a correlation between telomere length and organismal age within species, and correlations between telomere shortening rate and lifespan among species. This result presents the tantalizing possibility that telomere length could be used to provide much needed information on age, ageing and survival in natural populations where longitudinal studies are lacking. Here we review methods available for measuring telomere length and discuss the potential uses and limitations of telomeres as age and ageing estimators in the fields of vertebrate ecology, evolution and conservation

Investigation of Changes in Mechanical Properties and Microstructures of Mild Steel Joint by Oxy-Hydrogen Welding

In this paper, in order to see weldability behaviour of mild steels joint by the oxy-hydrogen welding technique, an investigation of changes in mechanical properties and microstructures of mild steel is carried out. The specimens with 1.5 mm thickness and 125 mm length are welded by using both the oxyhydrogen and oxy-acetylene gases. Microstructural analysis is carried out on the cross sectional area of the welding zone in order to compare the changes in weld metals and the heat affected zones in terms of the welding gases. Experimental results of the tensile strength and microhardness obtained with mechanical tests show that samples welded with oxy-acetylene exhibited 10% higher yield strength than that of the samples welded with oxy-hydrogen. However, the oxy-hydrogen welding technique can be used as an alternative to the oxy-acetylene welding, since it has advantageous of being clean and economic.С целью выяснения качества сварного шва на малоуглеродистой стали, полученного водород-кислородной сваркой, проведено исследование изменений механических свойств и микроструктуры данного типа стали. Образцы толщиной 1,5 мм и длиной 125 мм сваривались как водород-кислородной, так и ацетиленокислородной сваркой. Был проведён микроструктурный анализ поперечного сечения зоны сварки с целью сравнения изменений в металле шва и зоне термического влияния в зависимости от сварочного газа. Экспериментальные результаты по пределу прочности при растяжении и микротвёрдости, полученные при механических испытаниях, показали, что образцы, сваренные ацетиленокислородной сваркой, имеют прочность при растяжении на 10% выше, чем водород-кислородной. Однако водород-кислородная сварка может быть использована как альтернатива ацетиленокислородной сварке, так как она имеет преимущества по чистоте и экономичности.З метою з’ясування якости зварюваного шва на маловуглецевій сталі, виробленого водневокисневим зварюванням, виконано дослідження змін механічних властивостей і мікроструктури даного типу сталі. Зразки товщиною у 1,5 мм та довжиною у 125 мм зварювалися як водневокисневим, так і ацетиленокисневим зварюванням. Виконано мікроструктурну аналізу поперечного перерізу зони зварювання з метою порівняння змін у металі шва та зоні термічного впливу в залежності від зварювального газу. Експериментальні результати стосовно границі міцности при розтягу та мікротвердости, одержані під час механічних випробувань, показали, що зразки, зварені ацетиленокисневим зварюванням, мають міцність при розтягу, на 10% вищу, ніж зварені водневокисневою газовою сумішшю. Однак водневокисневе зварювання може бути використано як альтернатива ацетиленокисневому зварюванню завдяки його перевазі за чистотою та економічністю

Joinability of Particulate Reinforced Alumix 231 Based Composite Materials Produced by Powder Metallurgy Route

In the present study, weldability of Alumix 231 based composite materials reinforced with Al₂O₃ and B₄C and produced by powder metallurgy route is investigated. Various amounts of (0, 5, 10, and 15% wt.)Al₂O₃ and B₄C powders are added to the pre-alloyed Alumix 231 (Al—2.5% Cu—0.5% Mg—14% Si) powders separately and then mixed in a three-dimensional mixer for 45 min. Powders are compacted and sintered in an argon atmosphere at 640°C for 4 h. Produced blocks are welded by the Tungsten Inert Gas (TIG) welding at 25 V, 197 A and 14 l/min shielded gas flow in commercially pure argon atmosphere. Macro- and microexamination together with some mechanical properties of the welded area are studied. The results show that, although high amount of particles resulted in a decrease in the density, these composite materials can still be welded by the TIG welding method successfully.Исследуется свариваемость произведённых методом порошковой металлургии композитных материалов на основе Alumix 231, армированных Al₂O₃ и B₄C. Различные количества (0, 5, 10 и 15 масс.%) порошков Al₂O₃ и B₄C по отдельности добавлялись в предварительно сплавленные порошки Alumix 231 (Al—2,5% Cu—0,5% Mg—14% Si), а затем перемешивались в трёхмерном миксере в течение 45 минут. Затем порошки прессовались и спекались в атмосфере аргона при 640°C в течение 4 часов. Полученные блоки сваривались вольфрамовой сваркой в атмосфере инертного газа при 25 В, 197 А и потоке инертного газа в 14 л/мин в атмосфере технически чистого аргона. Были проведены макро- и микроисследования некоторых механических свойств сварной зоны. Их результаты показали, что, хотя оказалось большое количество частиц с пониженной плотностью, эти композитные материалы могут быть успешно сварены методом вольфрамовой сварки в атмосфере инертного газа.Досліджується зварюваність вироблених методою порошкової металургії композитних матеріялів на основі Alumix 231, армованих Al₂O₃ і B₄C. Різні кількості (0, 5, 10 і 15 мас.% ) порошків Al₂O₃ і B₄C окремо додавалися у попередньо стоплені порошки Alumix 231 (Al—2,5% Cu—0,5% Mg—14% Si), а потім перемішувалися в тривимірному міксері впродовж 45 хвилин. Потім порошки пресувалися та спікалися в атмосфері арґону при 640°C впродовж 4 годин. Одержані блоки зварювалися вольфрамовим зваренням в атмосфері інертного газу при 25 В, 197 А та потоці інертного газу у 14 л/хв. в атмосфері технічно чистого арґону. Було виконано макро- та мікродослідження деяких механічних властивостей зварної зони. їх результати показали, що, хоча виявилася велика кількість частинок зі зниженою густиною, ці композитні матеріяли можуть бути успішно зварені методою вольфрамового зварювання в атмосфері інертного газу

Aberrant Epigenetic Silencing Is Triggered by a Transient Reduction in Gene Expression

Aberrant epigenetic silencing plays a major role in cancer formation by inactivating tumor suppressor genes. While the endpoints of aberrant silencing are known, i.e., promoter region DNA methylation and altered histone modifications, the triggers of silencing are not known. We used the tet-off system to test the hypothesis that a transient reduction in gene expression will sensitize a promoter to undergo epigenetic silencing.The tet responsive promoter (P(TRE)) was used to drive expression of the selectable human HPRT cDNA in independent transfectants of an Hprt deficient mouse cell line. In this system, high basal HPRT expression is greatly reduced when doxycycline (Dox) is added to the culture medium. Exposure of the P(TRE)-HPRT transfectants to Dox induced HPRT deficient clones in a time dependent manner. A molecular analysis demonstrated promoter region DNA methylation, loss of histone modifications associated with expression (i.e., H3 lysine 9 and 14 acetylation and lysine 4 methylation), and acquisition of the repressive histone modification H3 lysine 9 methylation. These changes, which are consistent with aberrant epigenetic silencing, were not present in the Dox-treated cultures, with the exception of reduced H3 lysine 14 acetylation. Silenced alleles readily reactivated spontaneously or after treatment of cells with inhibitors of histone deacetylation and/or DNA methylation, but re-silencing of reactivated alleles did not require a new round of Dox exposure. Inhibition of histone deacetylation inhibited both the induction of silencing and re-silencing, whereas inhibition of DNA methylation had no such effect.This study demonstrates that a transient reduction in gene expression triggers a pathway for aberrant silencing in mammalian cells and identifies histone deacetylation as a critical early step in this process. DNA methylation, in contrast, is a secondary step in the silencing pathway under study. A model to explain these observations is offered

VEZF1 elements mediate protection from DNA methylation

There is growing consensus that genome organization and long-range gene regulation involves partitioning of the genome into domains of distinct epigenetic chromatin states. Chromatin insulator or barrier elements are key components of these processes as they can establish boundaries between chromatin states. The ability of elements such as the paradigm β-globin HS4 insulator to block the range of enhancers or the spread of repressive histone modifications is well established. Here we have addressed the hypothesis that a barrier element in vertebrates should be capable of defending a gene from silencing by DNA methylation. Using an established stable reporter gene system, we find that HS4 acts specifically to protect a gene promoter from de novo DNA methylation. Notably, protection from methylation can occur in the absence of histone acetylation or transcription. There is a division of labor at HS4; the sequences that mediate protection from methylation are separable from those that mediate CTCF-dependent enhancer blocking and USF-dependent histone modification recruitment. The zinc finger protein VEZF1 was purified as the factor that specifically interacts with the methylation protection elements. VEZF1 is a candidate CpG island protection factor as the G-rich sequences bound by VEZF1 are frequently found at CpG island promoters. Indeed, we show that VEZF1 elements are sufficient to mediate demethylation and protection of the APRT CpG island promoter from DNA methylation. We propose that many barrier elements in vertebrates will prevent DNA methylation in addition to blocking the propagation of repressive histone modifications, as either process is sufficient to direct the establishment of an epigenetically stable silent chromatin stat

The relation of the pleural thickening in tuberculosis pleurisy with the activity of adenosine deaminase

Selvi, A. Kant, S. Arslan, M. Ozgel. Background: Residual pleural thickening (RPT) still occurs in most patients with tuberculosis pleurisy despite advances in the treatment of tuberculosis. The aim of this study was to evaluate the significance of RPT in tuberculosis pleurisy with the patients clinical findings, biochemical and microbiological properties of pleural effusion and with the total adenosine deaminase (ADA) and isoenzymes levels. Methods: 121 tuberculosis pleurisy patients were evaluated retrospectively. According to posteroanterior chest x-rays, the 63 (52%) cases with the thickness 2 mm or more in lower lateral hemithorax were grouped as I and the 58 (48%) cases without pleural thickness were grouped as II. The amount of pleural effusion was classified into small, medium or massive according to their chest x-rays. In both groups; sex, age, symptoms score, bacteriological and biochemical tests and ADA levels were recorded. Results: 81 (67%) male and 40 (33%) female, overall 121 patients were enrolled into the study. RPT was found higher in males (p=0.014) and the increase ran parallel with the amount of cigarette smoking (p=0.014). RPT was found to be lower in small effusions (p=0.001). The group with RPT, the serum albumin was found lower (p=0.002), pleural fluid total protein (p=0.047) and the ratio of pleural fluid protein to serum protein (p=0.002) were found higher. In group I, total ADA: 69.5±38.9 IU/L and ADA2: 41.3±31.6 IU/L were higher than the cases without RPT (p=0.032, p=0.017, respectively). Conclusions: We suggest that the immunological mechanisms are effective in the development of pleural thickening