The Influence of CAT and Self-Disclosure on the Classroom

Epping-Fuentes, DarrenClassroom relational dynamics influence the ability to teach and learn effectively, making positive, meaningful relationships in the classroom crucial. Communication Accommodation Theory (CAT) explores how communicative adjustments, or accommodations, “reflect and affect personal and social relationships” (Soliz et al., 2021, p. 130). Thus, implementing CAT in the classroom could result in greater effectiveness of teaching and learning. Because CAT is used tomanage identity and relationships, it is also important to understand the results of communicating identity in the classroom. Communicating identity by self-disclosing can foster a connected classroom dynamic (Johnson & LaBelle, 2015), resulting in greater satisfaction with and appreciation of classroom relationships (Hosek, 2015). Because CAT, specifically accommodation behaviors and self-disclosure, influences academic performance and relational satisfaction, their usage in classrooms would bolster instructional communication. To understand the influence of CAT and self-disclosure on the classroom, a thorough literature review examining research on CAT’s classroom influence will be conducted first.Then, the implementation of self-disclosure in the classroom will be analyzed. Together, these two parts will explain the importance of CAT and self-disclosure’s usage in classrooms to increase instructional effectiveness and build positive relationships

Purification of Cathepsin D by Affinity Chromatography on Pepstatin Sepharose Column

A method was developed for the isolation of cathepsin D by affinity chromatography on immobilized pepstatin. This inhibitor was coupled to agarose by water soluble carbodiimide. Further purification included gel filtration on Sephadex G-100. The obtained cathepsin D exists in three active forms which were resolved on DEAE cellulose. Electrophoresis in the presence of sodium. dodecyl sulphate revealed that the first form consists of only one polypeptide chain having molecular weight 42 000. The second and the third form contain also polypeptides having molecular weight 27 000 and 14 000

Purification of Cathepsin D by Affinity Chromatography on Pepstatin Sepharose Column

A method was developed for the isolation of cathepsin D by affinity chromatography on immobilized pepstatin. This inhibitor was coupled to agarose by water soluble carbodiimide. Further purification included gel filtration on Sephadex G-100. The obtained cathepsin D exists in three active forms which were resolved on DEAE cellulose. Electrophoresis in the presence of sodium. dodecyl sulphate revealed that the first form consists of only one polypeptide chain having molecular weight 42 000. The second and the third form contain also polypeptides having molecular weight 27 000 and 14 000

Isolation of Cathepsin Band a-N-Benzoylarginine-B-naphthylamide Hydrolase by Covalent Chromatography on Activated Thiol Sepharose

Cathepsin Band a-N-benzoylarginine-P,-naphthylamide (BANA) hydrolase have been isolated from bovine lymph nodes using a novel procedure that includes besides gel filtration and ion exchange chromatography also covalent chromatography as the essential step. Both enzymes were selectively bound to the activated thiol-Sepharose and afterwards eluted with cysteine. The homogeneity of enzymes was proved by polyacrylamide gel electrophoresis

In vitro transfection of HeLa cells with temperature sensitive polycationic copolymers

Cataloged from PDF version of article.In this study, we investigated different types of polyethyleneimine (PEI) and their block copolymers with N-isopropylacrylamide (NIPA) as temperature-sensitive polycationic non-viral vectors for transfection of HeLa cells in cell culture media. First carboxyl-terminated poly(NIPA) was synthesized and then copolymerized with PEIs branched or linear and with two different molecular weights (2 and 25 kDa). Addition of PEI units to the poly(NIPA) chains increased the LCST values up to body temperature. Zeta potentials of the copolymers were significantly lower than the corresponding PEI homopolymers. A green fluorescent protein expressing plasmid was used as a model. Complexes of this plasmid both with PEIs and their copolymers were formed. The zeta potentials of these complexes were between -3.1 and +21.3. Higher values were observed for the complexes prepared with branched and higher molecular weight PEIs. Copolymerization caused a profound decrease in the positive charges. Particle sizes of the complexes were in the range of 190-1235 nm. Using high polymer/plasmid ratios caused aggregation. The smallest complexes were obtained with the copolymer prepared with branched PEI with 25-kDa molecular weight. Copolymers were able to squeeze plasmid DNA more at the body temperature. Cytotoxicity was observed with PEIs especially with the branched higher molecular weights. Copolymerization reduced the cytotoxicity. The best in vitro DNA uptake efficiency (70%) was achieved with the complex prepared with poly(NIPA)/PEI25B. However, poly(NIPA)/PEI25L was the most successful vector for an effective gene expression without any significant toxicity. © 2004 Elsevier B.V. All rights reserved

Some Properties of Thymus Cathepsin D

Cathepsin D has been purified from calf thymus using ammonium sulphate precipitation and gel chromatography on Se.phadex G-100. Preparative electrophoresis in polyacrylamide gel, used as the final step in the purification procedure, yielded four active forms of cathepsin D that dissociated further .into several polypepUde bands in the presence of sodium dodecyl sulphate. All four forms were stable over a range of pH from 4-11. They were completely inhibited by pepstatin whereas other metal ions had no appreciable effect upon their activity

On the effect of Ti on Oxidation Behaviour of a Polycrystalline Nickel-based Superalloy

Titanium is commonly added to nickel superalloys but has a well-documented detrimental effect on oxidation resistance. The present work constitutes the first atomistic-scale quantitative measurements of grain boundary and bulk compositions in the oxide scale of a current generation polycrystalline nickel superalloy performed through atom probe tomography. Titanium was found to be particularly detrimental to oxide scale growth through grain boundary diffusion