¿Puede la antropometría medir la grasa corporal de las personas que viven con el VIH/SIDA?: revisión sistemática

Introduction: Assessment of the quantity and distribution of body fat in people living with HIV/AIDS is of great importance in clinical practice, due to the association of body fat changes with clinical conditions. The aim of this systematic review was to answer the central question: Can anthropometry accurately measure the body fat in people living with HIV/AIDS?Material and Methods: Systematic review carried out using four databases: Medline, LILACS, Scopus and BDTD.Results: Of the 581 studies found, 11 met the eligibility criteria. To assess the validate of anthropometry, only two studies employed regress analysis to development of predictive body fat equations in people living with HIV/AIDS and nine studies employed correlation analysis. This coefficient only measures the strength of the relation between two variables, and there is not concordance between them and therefore, these studies did not accurately evaluate whether or not the anthropometric information showed good concordance with the gold standard. The other two studies developed five equations to evaluate the total fat and limbs (arm, leg and trunk) in people living with HIV/AIDS using antiretrovirals and showed R2 between 0.50 and 0.83.Conclusions: Further research needs to be conducted to answer the central question of this review, as the small number of articles that applied the correct statistical test and the absence of research on people living with HIV/AIDS without the use of antiretrovirals.Introducción: La evaluación de la cantidad y distribución de la grasa corporal en personas que viven con el VIH/SIDA es de gran importancia en la práctica clínica, debido a la asociación de los cambios de grasa corporal con condiciones clínicas. El objetivo de esta revisión es responder a la pregunta central: ¿Puede la antropometría medir con precisión la grasa corporal en las personas que viven con el VIH/SIDA?Material y Métodos: Revisión sistemática llevada a cabo por medio de cuatro bases de datos: Medline, LILACS, Scopus y BDTD.Resultados: De los 581 estudios encontrados, 11 cumplieron con los criterios de elegibilidad. Para evaluar la validación de la antropometría, sólo dos estudios emplearon análisis de regresión para el desarrollo de las ecuaciones de predicción de grasa corporal en las personas que viven con el VIH/SIDA y nueve estudios emplearon análisis de correlación. Este coeficiente sólo mide la fuerza de la relación entre dos variables, y no hay concordancia entre ellos y, por lo tanto, estos estudios no evaluaron con precisión si la información antropométrica mostró buena concordancia con el estándar de oro. Los otros dos estudios desarrollaron cinco ecuaciones para evaluar la grasa total y en las extremidades (brazos, piernas y tronco) en personas que viven con el VIH/SIDA y usan antirretrovirales y mostraron R2 entre 0,50 y 0,83.Conclusiones: Más investigación debe llevarse a cabo para responder a la pregunta central de esta revisión, dado el pequeño número de artículos en que se aplicó la prueba estadística correcta y la ausencia de investigaciones sobre personas que viven con el VIH/SIDA que no usan antirretrovirales

Diagnóstico tardio de infecção pelo Vírus da Imunodeficiência Humana e fatores associados

Objetivo: analisar a ocorrência de diagnóstico tardio de infecção pelo Vírus da Imunodeficiência Humana e seus fatores associados. Método: trata-se de estudo epidemiológico, transversal e analítico, realizado com 369 pessoas acompanhadas por Serviços de Assistência Especializada, em tratamento antirretroviral, entrevistadas por meio de questionário. Realizou-se análise univariada, utilizando-se os testes Qui-quadrado de Pearson ou exato de Fisher e o teste de Kruskall-Wallis, e análise multivariada, pelo modelo de regressão logística ordinal de chances proporcionais. Resultados: observou-se a ocorrência de 59,1% de diagnóstico tardio da infecção; a probabilidade de diagnóstico mais tardio é maior entre pessoas que têm parceria fixa, quando comparadas aos que não têm; com o aumento da idade, particularmente, acima dos 35 anos; entre os que possuem menor escolaridade; para os que buscam os serviços de saúde para realizar o teste de HIV quando se sentem doentes; para aqueles que fazem o teste de HIV com menor frequência ou nunca o fazem após relação sexual sem preservativo com parceria fixa. Conclusão: o conhecimento da elevada proporção de diagnóstico tardio e de seus fatores associados verificados neste estudo tornam imperiosos o planejamento e a implementação de novas políticas e estratégias que visem ao diagnóstico oportuno da infecção.Objetivo: analizar la ocurrencia del diagnóstico tardío de infección por el Virus de Inmunodeficiencia Humana y sus factores asociados. Método: estudio epidemiológico, transversal y analítico, realizado con 369 personas que utilizan Servicios de Asistencia Especializada, en tratamiento antirretroviral, entrevistados a través de un cuestionario. El análisis univariado se realizó por medio de la prueba de chi-cuadrado de Pearson o la prueba exacta de Fisher y la prueba de Kruskall-Wallis, mientras que para el análisis multivariado se utilizó el modelo de regresión logística ordinal de probabilidades proporcionales. Resultados: se observó la ocurrencia de 59,1% de diagnóstico tardío de la infección; la probabilidad de un diagnóstico posterior es mayor entre las personas que tienen una pareja estable, en comparación con quienes no la tienen; con el aumento de la edad, particularmente, por encima de los 35 años; entre aquellos con índice más bajo de escolaridad; para aquellos que buscan servicios de salud para hacerse una prueba de VIH cuando se sienten enfermos; para quienes se realizan la prueba del VIH con menor frecuencia o nunca lo hacen después de tener relaciones sexuales sin condón con una pareja estable. Conclusión: el conocimiento de la alta proporción de diagnósticos tardíos y sus factores asociados verificados en este estudio hacen que la planificación e implementación de nuevas políticas y estrategias dirigidas al diagnóstico oportuno de la infección sea imperativa.Objective: to analyze the occurrence of late diagnosis of infection by the Human Immunodeficiency Virus and its associated factors. Method: this is an epidemiological, cross-sectional and analytical study, carried out with 369 people followed-up by Specialized Assistance Services, undergoing anti-retroviral treatment, and interviewed by means of a questionnaire. Univariate analysis was performed using Pearson’s chi-square test or Fisher’s exact test and Kruskall-Wallis test, and multivariate analysis using the ordinal logistic regression model of proportional odds. Results: the occurrence of 59.1% for late diagnosis of the infection was observed; the probability of later diagnosis is greater among people who have a steady partnership, when compared to those who do not; with increasing age, particularly above 35 years old; among those with lower schooling; for those who seek the health services to have an HIV test when they feel sick; and for those who test HIV less often or never do it after sex without a condom with a steady partner. Conclusion: the knowledge on the high proportion of late diagnosis and its associated factors verified in this study make the planning and implementation of new policies and strategies aimed at the timely diagnosis of the infection imperative

Can anthropometry measure the body fat of people living with HIV/AIDS?: A systematic review

Introduction: Assessment of the quantity and distribution of body fat in people living with HIV/AIDS is of great importance in clinical practice, due to the association of body fat changes with clinical conditions. The aim of this systematic review was to answer the central question: Can anthropometry accurately measure the body fat in people living with HIV/AIDS? Material and Methods: Systematic review carried out using four databases: Medline, LILACS, Scopus and BDTD. Results: Of the 581 studies found, 11 met the eligibility criteria. To assess the validate of anthropometry, only two studies employed regress analysis to development of predictive body fat equations in people living with HIV/AIDS and nine studies employed correlation analysis. This coefficient only measures the strength of the relation between two variables, and there is not concordance between them and therefore, these studies did not accurately evaluate whether or not the anthropometric information showed good concordance with the gold standard. The other two studies developed five equations to evaluate the total fat and limbs (arm, leg and trunk) in people living with HIV/AIDS using antiretrovirals and showed R2 between 0.50 and 0.83. Conclusions: Further research needs to be conducted to answer the central question of this review, as the small number of articles that applied the correct statistical test and the absence of research on people living with HIV/AIDS without the use of antiretrovirals

Profile of patients diagnosed with AIDS at age 60 and above in Brazil, from 1980 until June 2009, compared to those diagnosed at age 18 to 59.

OBJECTIVES: The aim of this study was to learn more about people diagnosed with acquired immunodeficiency syndrome (AIDS) at age 60 and above in Brazil, and to compare them with people diagnosed at a younger age. METHODS: This study was based on the analysis of secondary data from the Brazilian AIDS Program. The study population consisted of people diagnosed with AIDS at age 60 and above. The comparison group was comprised of a 20% random sample of people diagnosed at age 18 to 59, frequency-matched by year of diagnosis. RESULTS: 544,846 cases of AIDS were reported in Brazil from 1980 until June 2009. Over 90% of cases were diagnosed between 18 and 59 years of age, and 13,657 (2.5%) at age 60 and above. The first case of AIDS among the elderly was reported in 1984. The comparison group consisted of 101,528 patients. Gender proportion was similar for both groups, and the proportion of people identified with AIDS after death in the Brazilian Mortality Information System (SIM) was 4% higher among the elderly. Both groups were also similar regarding the region of residence; a markedly higher proportion lived in Southeastern Brazil. Older people were more likely to have lower education and to have contracted AIDS by heterosexual contact, and less likely to be intravenous drug users. Male to female ratio among those diagnosed with AIDS at or above age 60 decreased over the years, in the same way as observed for the whole cohort. Mortality was higher among men in both groups. CD4 category (taken closest to the date of AIDS diagnosis) was very similar in both groups. CONCLUSION: The characteristics of the epidemic among the elderly show similarities to the younger group considering gender distribution and CD4 category, but differ regarding educational level and exposure category. Also, the elderly were more likely not to have their AIDS condition promptly diagnosed

Gordura corporal de crianças e adolescentes vivendo com HIV estimada por antropometria: revisão sistemática

Objetivo: Avaliar se, em comparação a absorciometria por raio X de dupla energia (DXA), a antropometria é um bom método para estimar a gordura corporal de crianças e adolescentes vivendo com HIV. Método: Revisão sistemática da literatura realizada nas bases eletrônicas de dados Medline®; LILACS®; SCOPUS® e Biblioteca Digital Brasileira de Teses ou Dissertações®. Resultados: Dos 363 estudos encontrados, quatro foram selecionados. Os estudos apontaram fracas correlações entre a dobra cutânea supra-íliaca (DCSI) em relação à gordura do tronco, braços e pernas avaliada pelo DXA. A circunferência da cintura também esteve fracamente associada à quantidade de gordura do tronco. A razão antropométrica entre as dobras cutâneas subescapular (DCSE) e DCSI, dividida pela soma das dobras cutâneas bicipital (DCB) e tricipital (DCT) [DCSI+DCSE/DCB+DCT] explicou 69% da variabilidade da razão tronco/membros medida pelo DXA. A DCSE associada ao sexo e idade em equações preditivas concordou em 80% com a quantidade de gordura troncular e 85% em gordura total medida pelo DXA. Conclusão: A aferição antropométrica por dobras cutâneas, aplicadas em razões ou equações

Autocoleta de swab nasofaríngeo e teste molecular em pool testing como estratégias para detecção de coronavírus da síndrome respiratória aguda grave 2 (SARS-CoV-2): viabilidade em estudantes de medicina da Universidade Federal de Minas Gerais, 2021

Objective: To show the feasibility of using combined nasopharyngeal swab auto-collection and pool testing to detect SARS-CoV-2 in epidemiological surveys. Methods: The study involved a sample of 154 students from the Universidade Federal de Minas Gerais, who performed the self-collection of the nasopharyngeal swab in individual booths without supervision. Molecular testing was performed using the pool testing technique. Results: Obtaining samples lasted about 5 minutes each. Analysis 6 was performed to detect endogenous RNA in 40 samples, and the results indicated that no failures resulted from self-collection. None of the pools detected the presence of viral RNA. The cost of performing the molecular test (RT-PCR) by pool testing with samples obtained by self-collection was about 10 times lower than with the usual methods. Conclusion: The investigated strategies showed to be economically feasible and valid for the research of SARS-CoV-2 in epidemiological surveys.Objetivo: Demostrar la viabilidad de utilizar el uso combinado de la autocollección de swabs nasofaríngeos y pool testing para la detección del SARS-CoV2 en encuestas epidemiológicas. Métodos: El estudio involucró a una muestra de 154 estudiantes de la Universidade Federal de Minas Gerais, quienes realizaron la autocolección del hisopo nasofaríngeo en cabinas individuales sin supervision. La prueba molecular se realizó utilizando la técnica de prueba de grupo. Resultados: La obtención de muestras duró unos 5 minutos por persona. Se realizó un análisis para detectar RNA endógeno en 40 muestras y los resultados indicaron que no hubo fallas derivadas de la autocolección. Ninguno de los grupos detectó la presencia de RNA viral. El costo de realizar una prueba molecular (RT-PCR) por pool con muestras obtenidas por auto-recolección fue aproximadamente 10 veces menor que con los métodos habituales. Conclusión: Las estrategias investigadas demonstraram ser económicamente viables y válidas para la investigación del SARS-CoV-2 en encuestas epidemiológicas.Objetivo: Demonstrar a viabilidade da utilização combinada da autocoleta de swab nasofaríngeo e pool testing para detecção do SARS-CoV-2 em inquéritos epidemiológicos. Métodos: O estudo envolveu amostra de 154 estudantes da Universidade Federal de Minas Gerais, que realizaram a autocoleta do swab nasofaríngeo em cabines individuais e sem supervisão. O teste molecular foi realizado utilizando-se a técnica de pool testing. Resultados: A obtenção de amostras durou cerca de 5 minutos por pessoa. Realizou-se análise para detecção de RNA endógeno em 40 amostras e os resultados indicaram que não houve falhas decorrentes da autocoleta. Nenhum dos pools detectou presença de RNA viral. O custo da realização do teste molecular (RT-PCR) por pool testing com amostras obtidas por autocoleta foi cerca de dez vezes menor do que nos métodos habituais. Conclusão: As estratégias investigadas mostraram-se economicamente viáveis e válidas para a pesquisa de SARS-CoV-2 em inquéritos epidemiológicos

Trends and predictors of HIV-1 acquired drug resistance in Minas Gerais, Brazil: 2002–2012

Several studies show that the prevalence of multidrug-resistant HIV-1 virus is declining over time. A retrospective cohort study was carried out to evaluate the trends of drug resistance in antiretroviral treatment-exposed individuals in a state of a middle-income country, Minas Gerais, southeast region of Brazil. We analyzed 2115 HIV-1 sequences from 2002 up to 2012, from 52 cities of Minas Gerais. The groups were analyzed according to the definitions: “IAS – 3 class mutations”, if ≥1 drug resistance mutation from IAS 2015 list (DRM) was present in each class; “No fully susceptible drugs” as the absence of any fully susceptible drug in Stanford algorithm; and “GSS ≥ 2″, when a maximum calculated GSS (genotypic susceptibility score) was ≥2 or ≥3, counting only drugs available in Brazil and USA at given calendar years. Time trends of resistance were analyzed by Cochran–Armitage test. We observed a decrease in the rate resistance mutations for PI, NRTI, “IAS – 3 class mutations”, and “No fully susceptible drugs” over these 11 years, from 69.2% to 20.7%, 92.3% to 90.2%, 46.2% to 22.5%, and 12.8% to 5.7%, respectively (p < 0.05). Resistance to NNRTI increased from 74.4% to 81.6%, mainly because of K103N mutation. The GSS score ≥2 increased during the years from 35.9% to 87.3% (p < 0.001). We demonstrate that resistance to PI and to the three main classes simultaneously are declining, although the number of patients on of antiretroviral therapy has doubled in the last ten years in Brazil (125,000 in 2002 to 400,000 in 2014). Broader resistance testing and the availability of more therapeutic options might have influenced this decline. The increase in NNRTI resistance can limit this class as first line treatment in Brazil in the future. Keywords: HIV, Antiretroviral, Resistance, Genotyping, Epidemiolog