What Plants and Animals Do Early Childhood and Primary Students’ Name? Where Do They See Them?

Children from England and the United States of America have a basic similar knowledge of plants and animals, which they observe during their everyday life. Nine children of ages 4, 6, 8, and 10 years, in each country, were asked to free-list plants and animals. Afterwards, they were interviewed individually about the plants and animals they listed to determine where they were seen. Additionally, children were asked to name animals they knew that were found in specific habitats or had specific characteristics. The results showed that children from the earliest years notice the animals in their everyday lives and 8 year olds were able to name the most animals. Plants were not named as often as animals and children in the USA found it difficult to name plants when questioned. This study shows that children are in touch with their everyday environment to varying extents, and that rich experiences can greatly contribute to their knowledge about plants and animals

An Experimental Investigation of Conformational Fluctuations in Proteins G and L

SummaryThe B1 domains of streptococcal proteins G and L are structurally similar, but they have different sequences and they fold differently. We have measured their NMR spectra at variable temperature using a range of concentrations of denaturant. Many residues have curved amide proton temperature dependence, indicating that they significantly populate alternative, locally unfolded conformations. The results, therefore, provide a view of the locations of low-lying, locally unfolded conformations. They indicate approximately 4–6 local minima for each protein, all within ca. 2.5 kcal/mol of the native state, implying a locally rough energy landscape. Comparison with folding data for these proteins shows that folding involves most molecules traversing a similar path, once a transition state containing a β hairpin has been formed, thereby defining a well-populated pathway down the folding funnel. The hairpin that directs the folding pathway differs for the two proteins and remains the most stable part of the folded protein

Structural Basis for the Recognition of Cellular mRNA Export Factor REF by Herpes Viral Proteins HSV-1 ICP27 and HVS ORF57

The herpesvirus proteins HSV-1 ICP27 and HVS ORF57 promote viral mRNA export by utilizing the cellular mRNA export machinery. This function is triggered by binding to proteins of the transcription-export (TREX) complex, in particular to REF/Aly which directs viral mRNA to the TAP/NFX1 pathway and, subsequently, to the nuclear pore for export to the cytoplasm. Here we have determined the structure of the REF-ICP27 interaction interface at atomic-resolution and provided a detailed comparison of the binding interfaces between ICP27, ORF57 and REF using solution-state NMR. Despite the absence of any obvious sequence similarity, both viral proteins bind on the same site of the folded RRM domain of REF, via short but specific recognition sites. The regions of ICP27 and ORF57 involved in binding by REF have been mapped as residues 104–112 and 103–120, respectively. We have identified the pattern of residues critical for REF/Aly recognition, common to both ICP27 and ORF57. The importance of the key amino acid residues within these binding sites was confirmed by site-directed mutagenesis. The functional significance of the ORF57-REF/Aly interaction was also probed using an ex vivo cytoplasmic viral mRNA accumulation assay and this revealed that mutants that reduce the protein-protein interaction dramatically decrease the ability of ORF57 to mediate the nuclear export of intronless viral mRNA. Together these data precisely map amino acid residues responsible for the direct interactions between viral adaptors and cellular REF/Aly and provide the first molecular details of how herpes viruses access the cellular mRNA export pathway

Intercentre reproducibility of cardiac apparent diffusion coefficient and fractional anisotropy in healthy volunteers

BACKGROUND: Diffusion tensor cardiac magnetic resonance (DT-CMR) enables probing of the microarchitecture of the myocardium, but the apparent diffusion coefficient (ADC) and fractional anisotropy (FA) reported in healthy volunteers have been inconsistent. The aim of this study was to validate a stimulated-echo diffusion sequence using phantoms, and to assess the intercentre reproducibility of in-vivo diffusion measures using the sequence. METHODS AND RESULTS: A stimulated-echo, cardiac-gated DT-CMR sequence with a reduced-field-of-view, single-shot EPI readout was used at two centres with 3 T MRI scanners. Four alkane phantoms with known diffusivities were scanned at a single centre using a stimulated echo sequence and a spin-echo Stejskal-Tanner diffusion sequence. The median (maximum, minimum) difference between the DT-CMR sequence and Stejskal-Tanner sequence was 0.01 (0.04, 0.0006) × 10(-3) mm(2)/s (2%), and between the DT-CMR sequence and literature diffusivities was 0.02 (0.05, 0.006) × 10(-3) mm(2)/s (4%). The same ten healthy volunteers were scanned using the DT-CMR sequence at the two centres less than seven days apart. Average ADC and FA were calculated in a single mid-ventricular, short axis slice. Intercentre differences were tested for statistical significance at the p < 0.05 level using paired t-tests. The mean ADC ± standard deviation for all subjects averaged over both centres was 1.10 ± 0.06 × 10(-3) mm(2)/s in systole and 1.20 ± 0.09 × 10(-3) mm(2)/s in diastole; FA was 0.41 ± 0.04 in systole and 0.54 ± 0.03 in diastole. With similarly-drawn regions-of-interest, systolic ADC (difference 0.05 × 10(-3) mm(2)/s), systolic FA (difference 0.003) and diastolic FA (difference 0.01) were not statistically significantly different between centres (p > 0.05), and only the diastolic ADC showed a statistically significant, but numerically small, difference of 0.07 × 10(-3) mm(2)/s (p = 0.047). The intercentre, intrasubject coefficients of variance were: systolic ADC 7%, FA 6%; diastolic ADC 7%, FA 3%. CONCLUSIONS: This is the first study to demonstrate the accuracy of a stimulated-echo DT-CMR sequence in phantoms, and demonstrates the feasibility of obtaining reproducible ADC and FA in healthy volunteers at separate centres with well-matched sequences and processing

Characterisation of Sticky Debris Generated During Smear Wear

Smear wear behaviour has often been observed during rubber abrasion especially under mild test severities. It generates degraded sticky rubber debris, which often produces erratic measurements of abrasion weight loss. Various practical methods to avoid or remove the debris from the abrasion test surface have been reported, such as applying a drying powder lubricant. However, the detailed mechanism of smear wear behaviour is still not clear. In this paper, various characterisation techniques are applied to investigate the smear wear of both an unfilled NR model compound and a commercial carbon black filled SBR tyre tread compound obtained during blade abrasion testing. The debris showed lower molecular weight and higher oxygen content than the virgin materials. In addition, 75% of the smear wear was found to be de-crosslinked during smear wear, as detected by the DQ-NMR technique. For the first time, it is demonstrated that both the polymer itself and crosslinking points are broken down during smear wear. The effect of the smear layer on friction and abrasion is also discussed

PHL 1445: An eclipsing cataclysmic variable with a substellar donor near the period minimum

PublishedThis is a pre-copyedited, author-produced PDF of an article accepted for publication in Monthly Notices of the Royal Astronomical Society following peer review. The version of record is available online via the DOI in this record.We present high-speed, three-colour photometry of the eclipsing dwarf nova PHL 1445, which, with an orbital period of 76.3 min, lies just below the period minimum of ~82 min for cataclysmic variable stars (CVs). Averaging four eclipses reveals resolved eclipses of the white dwarf and bright spot. We determined the system parameters by fitting a parametrized eclipse model to the averaged light curve. We obtain a mass ratio of q = 0.087 ± 0.006 and inclination i = 85°.2 ± 0°.9. The primary and donor masses were found to be Mw = 0.73 ± 0.03 M⊙ and Md = 0.064 ± 0.005 M⊙, respectively. Through multicolour photometry a temperature of the white dwarf of Tw = 13 200 ± 700 K and a distance of 220 ± 50 pc were determined. The evolutionary state of PHL 1445 is uncertain. We are able to rule out a significantly evolved donor, but not one that is slightly evolved. Formation with a brown dwarf donor is plausible, though the brown dwarf would need to be no older than 600 Myr at the start of mass transfer, requiring an extremely low mass ratio (q = 0.025) progenitor system. PHL 1445 joins SDSS 1433 as a sub-period minimum CV with a substellar donor. The existence of two such systems raises an alternative possibility that current estimates for the intrinsic scatter and/or position of the period minimum may be in error.UK Science and Technology Facilities Council (STFC)FONDECY