Tumor Suppressor Gene Alterations In Patients With Malignant Mesothelioma Due To Environmental Asbestos Exposure In Turkey

Background Environmental asbestos exposure can cause the grave lung and pleura malignancies with a high mortality rate, and it is also associated with increased rate of other organ malignancies. Asbestos exposure can develop genotoxic effects and damage in the pleura and lungs. Objective In this study, we aimed to determine tumor suppressor gene (TSG) loss in genomic DNA which was isolated from pleural fluid and blood samples of patients with Malignant Pleural Mesothelioma (MPM) due to environmental asbestos exposure. Design and patients Prospective study of period from 2001 to 2003 in 17 patients with MPM. Methods A total of 12 chromosomal regions were researched by comparing genomic DNA samples isolated from blood and pleural effusion (using PCR, and polyacrilamid gel electrophoresis denaturizing), on 2 different chromosomes which have 9 different polymorphic determinants at 6q and 3 different polymorphic determinants at 9p using molecular genetic methods on 13 patients clinico-pathologically diagnosed MPM. Results Loss of Heterozygosity (LOH) was determined at D6S275 in one patient, at D6S301 in another, at D6S474 in 2, at ARG1 in 2, at D6S1038 in 2 and at D6S1008 in 3 patients. In 7 (54%) of the13 patients, we found LOH in at least one site. No LOH was determined at any informative loci in 6 patients. Of the 13 patients, no investigated markers were determined at 9p. Conclusion In this study, genomic DNA samples obtained from MPM patients with asbestos exposure revealed that they contained important genotoxic damage. We found no other study on this subject at molecular level in pleural effusion either in Turkey or in the med-line literature. We believe that this study will provide important support for other research into molecular-genetic variations, both on this subject and other malignancies, and may also constitute a base for early diagnosis and gene therapy research in the future.PubMedScopu

Factors influencing influenza and pneumococcal immunization rates of COPD patients in Bolu, Turkey

The aim of this study was finding out the rates of pneumococcal and influenza vaccination, the factors influencing vaccination, and non-vaccination in COPD patients. The study was conducted with 104 COPD patients diagnosed by a pulmonologist. The diagnosis was made according to the GOLD 2019 document via an FEV1/FVC ratio below 70%. The patients were inquired about getting vaccinated for influenza in the last year and pneumococci in the last five years. The patients were then grouped according to their vaccination status and inquired about their perception of the necessity of vaccination. The study revealed a significant association between COPD levels and the awareness of the necessity of annual influenza vaccination (p=0.019). However, there was no significant association between COPD levels and being vaccinated for influenza in the last year (p=0.434). There was no significant association between COPD levels and pneumococcal vaccination in the last five years or the awareness for the necessity of the vaccine (p=0.0559 and p=0.495). 22.2% of the patients who were vaccinated for pneumonia were also vaccinated for influenza and 98.7% of the patients who were not vaccinated for pneumonia were also not vaccinated for influenza in the last year. 85.7% of the patients who got an influenza vaccine in the last year also knew the necessity of getting annually vaccinated (p= [Med-Science 2020; 9(1.000): 60-6

Effects of Quadriceps Muscle Strength and Kinesiophobia on Health Related Quality of Life in Elderly Women and Men with Chronic Obstructive Pulmonary Disease

ozel, alp/0000-0002-1215-7051WOS: 000450536200013Objective: The aim of this study was to investigate the effects of quadriceps muscle strength and kinesiophobia on health-related quality of life in elderly women and men with chronic obstructive pulmonary disease. Methods: After recording the socio -demographic information of individuals, the Visual Analogue Scale (VAS) for pain status, the modified Medical Research Council Scale (mMRCS) for dyspnea, TAMPA Scale for Kinesiophobia (TSK), Saint George Respiratory Questionnaire (SGRQ) for health-related quality of life, and manual dynamometer were used to measure for muscle strength of quadriceps. Results: Seventeen (37.8%) of the 45 participants were female. The mean age and body mass index of females were 71.59 +/- 4.84 years and 28.75 +/- 6.71 kg/m(2), respectively. Kinesiophobia averages were 47.47 +/- 7.40 and 41.89 +/- 7.65 in women and men, respectively, and SGRQ((Symptom)) scores were 75.97 +/- 15.87 and 63.89 +/- 18.54 in women and men, respectively. Statistical analysis showed that there was a significant difference between men and women between kinesiophobia and SGRQ((symptom)) (p0.05). Conclusion: In elderly individuals with COPD, kinesiophobia is an important factor affecting the quality of life and is recommended to increase physical activity, and monitor the physiological, emotional well-being in order to improve quality of life

Protective effect of octreotide on intra-tracheal bleomycin-induced oxidative damage in rats

The present study is aimed at determining the effect of parenteral octreotide against oxidative damage caused by intra-tracheal bleomycin (BLM) administration. A total of 30 male Wistar rats randomly divided into three groups (control, bleomycin alone, and bleomycin and octreotide) were used in the study. A group of animals received a single dose of intra-tracheal bleomycin (7.5 mg/kg). Animals in another group, which also received intra-tracheal bleomycin, were given 82.5 mu g/kg octreotide via i.m. injection for a week. Animals in the control group received neither bleomycin nor octreotide. All animals were sacrificed at the end of the experiment. Serum levels of malondialdehyde, vitamins A, E, and C, selenium levels were determined. In addition, glutathion peroxidase activity levels in erythrocytes were also determined. Malondialdehyde levels and glutathion peroxidase activity were increased whereas antioxidant vitamin levels were decreased significantly in animals that received only bleomycin compared to control animals (pThe present study is aimed at determining the effect of parenteral octreotide against oxidative damage caused by intra-tracheal bleomycin (BLM) administration. A total of 30 male Wistar rats randomly divided into three groups (control, bleomycin alone, and bleomycin and octreotide) were used in the study. A group of animals received a single dose of intra-tracheal bleomycin (7.5mg/kg). Animals in another group, which also received intra-tracheal bleomycin, were given 82.5 microg/kg octreotide via i.m. injection for a week. Animals in the control group received neither bleomycin nor octreotide. All animals were sacrificed at the end of the experiment. Serum levels of malondialdehyde, vitamins A, E, and C, selenium levels were determined. In addition, glutathion peroxidase activity levels in erythrocytes were also determined. Malondialdehyde levels and glutathion peroxidase activity were increased whereas antioxidant vitamin levels were decreased significantly in animals that received only bleomycin compared to control animals (p&lt;0.05). The values in rats that received bleomycin and octreotide were found to be closer to the control group (p&lt;0.05). Selenium levels in animals that received only bleomycin were determined to be reduced compared to controls (p&lt;0.05). On the other hand, selenium levels in bleomycin and octreotide groups were similar to control values in (p&lt;0.05). In conclusion, bleomycin induces a severe stress and more importantly increases the amount of free radicals whereas octreotide administration reduces this oxidative damage significantly.</p

The effect of octreotide, an analog of somatostatin, on bleomycin-induced interstitial pulmonary fibrosis in rats

In this study, octreotide (OCT), a synthetic sonnatostatin analog, was tested for its beneficial effects in the prevention of interstitial pulmonary fibrosis (IPF) induced by bleomycin (BLM) in rats by histological examination and by evaluating tissue OH-proline levels. Thirty male Wistar rats were divided randomly into three groups: group I: intratracheal (i.t.) BLM (7.5 mg/kg, single dose) + saline solution [0.9% NaCl, subcutaneously (s.c.), once-daily for 7 days]; group II: i.t. BLM (7.5 mg/kg, single dose) + OCT acetate (82.5 mu g/kg, s.c., once-daily for 7 days); and the control group. At the end of the 7 days, lung tissues were excised and examined by histopathological methods. Levels of tissue hydroxyproline (OH-proline) were determined. BLM administration resulted in prominent histopathologic findings, such as diffuse alveolar damage and interstitial pulmonary fibrosis, as well as a significant increase in OH-proline level, as compared to controls. OCT application explicitly attenuated the histopathologic changes to a significant extent. OCT decreased paranchymal fibrosis and structural deformities in BLM-induced lung fibrosis. These results suggest that OCT administration to rats with BLM-induced IPF has a protective effect. Further studies are necessary to reveal the molecular mechanism(s) of OCT-induced protective effect