Spatial and temporal expression profiling of cell-wall invertase genes during early development in hybrid poplar

Cell-wall invertase genes are spatially and temporally regulated in several plant species, including Daucus carota L., Lycopersicon esculentum L. and Solanum tuberosum L. However, few studies of cell-wall invertase genes of trees have been conducted, despite the importance of trees as a source of lignocellulosic biopolymers.We identified three putative cell-wall invertase genes in hybrid poplar (Populus alba L. × grandidentata Michx.) that showed higher homology to each other than to cell-wall invertases of other dicotyledonous species, with two of the genes (Pa×gINV2 and Pa×gINV3) appearing as a genomic tandem repeat. These genes are more similar to each other than to tandemly repeated cell-wall invertases of other plants, perhaps indicating parallel evolution of a duplication event with cell-wall invertases in dicotyledons. Spatial and temporal expression analyses throughout a complete annual cycle indicated that Pa×gINV1 and Pa×gINV2 are highly regulated in vegetative tissues during three distinct growth phases: early growth, dormancy and post-dormancy. Expression of the third gene (Pa×gINV3) appears to be tightly regulated and may represent a floral-specific cell-wall invertase. Of the two genes expressed in vegetative tissues, Pa×gINV1 appears to be exclusively involved in processes related to dormancy, whereas Pa×gINV2 appears to encode an enzyme involved in phloem unloading and in providing actively growing tissues, such as developing xylem, with the energy and carbon skeletons necessary for respiration and cell wall biosynthesis

Hydrological control of soil thickness spatial variability on the initiation of rainfall-induced shallow landslides using a three-dimensional model

Thickness and stratigraphic settings of soils covering slopes potentially control susceptibility to initiation of rainfall-induced shallow landslides due to their local effect on slope hydrological response. Notwithstanding the relevance of the assessment of hazard to shallow landsliding at a distributed scale by approaches based on a coupled modelling of slope hydrological response and slope stability, the spatial variability of soil thickness and stratigraphic settings are factors poorly considered in the literature. Under these premises, this paper advances the well-known case study of rainfall-induced shallow landslides involving ash-fall pyroclastic soils covering the peri-Vesuvian mountains (Campania, southern Italy). In such a unique geomorphological setting, the soil covering is formed by alternating loose ash-fall pyroclastic deposits and paleosols, with high contrasts in hydraulic conductivity and total thickness decreasing as the slope angle increases, thus leading to the establishment of lateral flow and an increase of pore water pressure in localised sectors of the slope where soil horizon thickness is less. In particular, we investigate the effects, on hillslope hydrological regime and slope stability, of irregular bedrock topography, spatial variability of soil thickness and vertical hydraulic heterogeneity of soil horizons, by using a coupled three-dimensional hydrological and a probabilistic infinite slope stability model. The modelling is applied on a sample mountain catchment, located on Sarno Mountains (Campania, southern Italy), and calibrated using physics-based rainfall thresholds derived from the literature. The results obtained under five simulated constant rainfall intensities (2.5, 5, 10, 20 and 40 mm h−1) show an increase of soil pressure head and major failure probability corresponding to stratigraphic and morphological discontinuities, where a soil thickness reduction occurs. The outcomes obtained from modelling match the hypothesis of the formation of lateral throughflow due to the effect of intense rainfall, which leads to the increase of soil water pressure head and water content, up to values of near-saturation, in narrow zones of the slope, such as those of downslope reduction of total soil thickness and pinching out of soil horizons. The approach proposed can be conceived as a further advance in the comprehension of slope hydrological processes at a detailed scale and their effects on slope stability under given rainfall and antecedent soil hydrological conditions, therefore in predicting the most susceptible areas to initiation of rainfall-induced shallow landslides and the related I-D rainfall thresholds. Results obtained demonstrate the occurrence of a slope hydrological response depending on the spatial variability of soil thickness and leading to focus slope instability in specific slope sectors. The approach proposed is conceived to be potentially exportable to other slope environments for which a spatial modelling of soil thickness would be possible

Probabilistic approaches for assessing rainfall thresholds triggering shallow landslides. The study case of the peri-vesuvian area (Southern Italy)

Ash-fall pyroclastic soil deposits covering steep carbonate slopes in the peri-Vesuvian area (southern Italy) are periodically involved in shallow landslides (about 700 events were recorded during the last three centuries, as reported by CASCINI et alii, 2008), triggered by intense and/or prolonged rainfall events, which evolve as catastrophic debris flows. In the last decades, many studies have been focused on estimating reliable relationships among the triggering of shallow landslides and the amount and duration of rainfall events, as well as the role played by antecedent soil hydrological conditions. Results of these studies have been expected to give information on temporal hazard to landslide onset to be used for setting a reliable early warning system. In this paper we present probabilistic approaches to assess rainfall thresholds triggering shallow landslides by classical empirical methods and to manage the uncertainties related to biases of data. At this scope, rainfall events related to the occurrence of debris flows along slopes of the Sarno and Lattari Mountains, known from chronicles of the last century, were analyzed by means of the empirical models of Intensity-Duration (I-D) (CAINE, 1980) and rainfall recorded in the day of the landslide occurrence (P) vs the antecedent cumulated rainfall (Pa) (CROZIER & EYLES, 1980). In order to limit and to assess uncertainties related to biases of rainfall data, a comparison with the regional probability model of high intensity rainfall, carried out in the framework of the VAPI Project (ROSSI & VILLANI, 1994) has been carried out. Moreover, rainfall data were processed by a bivariate logistic regression model resulting in the assessment of probability to landslide triggering, given an assumed rainfall event. The I-D empirical rainfall thresholds obtained by Caine model (1980) were compared to rainfall thresholds estimated by deterministic approaches (DE VITA et alii, 2013; NAPOLITANO et alii, 2016) showing a good match

Seasonal and event-based hydrological and slope stability modeling of pyroclastic fall deposits covering slopes in Campania (Southern Italy)

The pyroclastic fall deposits mantling mountain slopes in the Campania region (Southern Italy) represent one of the most studied geomorphological frameworks of the world regarding rainfall-induced debris flows threating urban areas. The proposed study focused on advancing knowledge about the hydrological response of pyroclastic fall coverings from the seasonal to event-based time scales, leading to the initiation of slope instability. The study was based on two consequential tasks. The first was the analysis of a six-year monitoring of soil pressure head carried out in a sample area of the Sarno Mountains, located above a debris flow initiation zone. The second was based on coupled hydrological and slope stability modeling performed on the physical models of slopes, which were reconstructed by empirical correlations between the slope angle, total thickness, and stratigraphic settings of pyroclastic fall deposits mantling slopes. The results obtained were: (a) The understanding of a soil pressure head regime of the volcaniclastic soil mantle, always ranging in unsaturated conditions and characterized by a strong seasonal variability depending on precipitation patterns and the life cycle of deciduous chestnut forest; and (b) the reconstruction through a deterministic approach of seasonal intensity-duration rainfall thresholds related to different morphological conditions

Impact of the laminar flame speed correlation on the results of a quasi-dimensional combustion model for Spark-Ignition engine

Abstract In the present study, the impact of the laminar flame speed correlation on the prediction of the combustion process and performance of a gasoline engine is investigated using a 1D numerical approach. The model predictions are compared with experimental data available for full- and part-load operations of a small-size naturally aspirated Spark-Ignition (SI) engine, equipped with an external EGR circuit. A 1D model of the whole engine is developed in the GT-Power™ environment and is integrated with refined sub-models of the in-cylinder processes. In particular, the combustion is modelled using the fractal approach, where the burning rate is directly related to the laminar flame speed. In this work, three laminar flame speed correlations are assessed, including both experimentally- and numerically-derived formulations, the latter resulting from the fitting of laminar flame speeds computed by a chemical kinetic solver. Each correlation is implemented within the combustion sub-model, which is properly tuned to reproduce the experimental performance of the engine at full load. Then, the reliability of the considered flame speed formulations is proved at part-loads, even under external EGR operations

A Large Nonmetastatic Anaplastic Thyroid Cancer with Complete Thyroidal Confinement

Anaplastic thyroid cancer (ATC) is rare but extremely aggressive, which accounts for about 2% of all thyroid cancers yet nearly 50% of thyroid-cancer-associated deaths in the United States. The median survival time from diagnosis is 5 months, with a 1-year survival rate of only 20%. We report here a case of ATC in a 56-year-old man who survived a large ATC. Preoperative fine-needle aspiration biopsy study to a large right thyroid mass suggested ATC. Total thyroidectomy with radical lateral neck and central neck dissection removed a well-circumscribed 9.5 cm tumor without extrathyroidal extension or lymphovascular invasion. All 73 lymph nodes removed were negative for metastasis. The tumor consisted of highly pleomorphic, undifferentiated cells with large zones of necrosis and loss of thyroid transcription factor-1 and thyroglobulin expression. A focal well-differentiated component and PAX8 expression confirmed its thyroid follicular cell origin. Nine months after postsurgical adjuvant concurrent radiation therapy and chemotherapy, the patient remained well without clinical, biochemical, and radiographical evidence for cancer recurrence. This is an unusual case of ATC in that it is one of the largest ATC tumors reported to display mild pathologic behavior and relatively long-term patient survival

Transoral robotic-assisted thyroidectomy with central neck dissection: preclinical cadaver feasibility study and proposed surgical technique

Recently, a transoral robotic-assisted technique to access the thyroid gland has been introduced. Despite the advantages this approach may have over other minimally invasive and robotic-assisted techniques, we found that the placement of the camera through the floor of mouth led to restricted freedom of movement. We describe our modification to this technique to overcome this problem. In a study using two fresh human cadavers, the camera port of the da Vinci robot was placed in the midline oral vestibule instead of the floor of the mouth. A transoral thyroidectomy and central neck dissection was successfully performed. Our modification led to an unfettered view of the central neck and allowed for a total thyroidectomy and central neck dissection. Our modification of transoral robotic-assisted thyroidectomy provides superior access to the central compartment of the neck over other robotic-assisted thyroidectomy techniques

Groundwater vulnerability of principal aquifers of the Campania region (southern Italy)

The assessment of groundwater vulnerability is an important aspect of territorial planning aimed at the management and protection of groundwater quality. This topic is particularly relevant for the Campania region (southern Italy) due to the abundance of groundwater resources and the strong dependence on them of current economic, social and environmental settings. The region is characterized by complex geological, structural and hydrogeological frameworks which make challenging and innovative the assessment of groundwater vulnerability with SINTACS, a parametric method officially recognized by the Italian environmental agencies. In order to apply results obtained to current regional regulations, groundwater vulnerability was estimated for the 80 principal aquifers, hosting respective groundwater bodies, as recognized by the application of the Directive 2000/60/EC. Among principal results, the alluvial and limestone (karst) aquifers, which are the most productive of the region, show the highest groundwater vulnerability, even with spatially variable conditions depending on local hydrogeological features

On the Temporality of Introducing Code Technical Debt

Code Technical Debt (TD) is intentionally or unintentionally created when developers introduce inefficiencies in the codebase. This can be attributed to various reasons such as heavy work-load, tight delivery schedule, unawareness of good practices, etc. To shed light into the context that leads to technical debt accumulation, in this paper we investigate: (a) the temporality of code technical debt introduction in new methods, i.e., whether the introduction of technical debt is stable across the lifespan of the project, or if its evolution presents spikes; and (b) the relation of technical debt introduction and the development team’s workload in a given period. To answer these questions, we perform a case study on twenty-seven Apache projects, and inspect the number of Technical Debt Items introduced in 6-month sliding temporal windows. The results of the study suggest that: (a) overall, the number of Technical Debt Items introduced through new code is a stable metric, although it presents some spikes; and (b) the number of commits performed is not strongly correlated to the number of introduced Technical Debt Items

Update of Radiofrequency Ablation for Treating Benign and Malignant Thyroid Nodules. The Future Is Now

Thermal and chemical ablation are minimally invasive procedures that avoid removal of the thyroid gland and target symptomatic nodules directly. Internationally, Radiofrequency ablation (RFA) is among one of the most widely used thermal ablative techniques, and is gaining traction in North America. Surgery remains the standard of care for most thyroid cancer, and in the right clinical setting, Active Surveillance (AS) can be a reasonable option for low risk disease. Minimally invasive techniques have emerged as an alternative option for patients deemed high risk for surgery, or for those patients who wish to receive a more active treatment approach compared to AS. Herein, we review the literature on the safety and efficacy of RFA for treating benign non-functioning thyroid nodules, autonomously functioning thyroid nodules, primary small low risk thyroid cancer (namely papillary thyroid cancer) as well as recurrent thyroid cancer.Peer reviewe