181 research outputs found
The research of qualitative indicators of gas pipelines during the operation
The operation of gas pipelines is a complex of technical measures, aimed at preservation of the main stock of gas pipeline transportation facilities. The purpose of these measures is to maintain and to restore the initial operational capabilities of gas pipelines, in general and in particular areas. The line section of gas pipelines has the largest size and cost. Naturally determined process of changing the quality of gas pipelines during the operation is accompanied by the accumulation and development of damages, failures; this determines the objective need to restore the quality of operation. The practical running of the line section of gas pipelines is characterized by a certain flow of failures; therefore it is necessary to ensure the required level of reliability of the gas pipeline during the entire period of operation. The quality control study of the inter-settlement gas pipeline section was conducted, and graphs of dependence of gas pipeline quality parameters on time were presented in the work.Keywords: line section of the gas pipeline, technological state, failures in operation, gas pipeline operation, control and quality parameters of the gas pipeline
Splitting fields and general differential Galois theory
An algebraic technique is presented that does not use results of model theory
and makes it possible to construct a general Galois theory of arbitrary
nonlinear systems of partial differential equations. The algebraic technique is
based on the search for prime differential ideals of special form in tensor
products of differential rings. The main results demonstrating the work of the
technique obtained are the theorem on the constructedness of the differential
closure and the general theorem on the Galois correspondence for normal
extensions..Comment: 33 pages, this version coincides with the published on
ΠΠ΅Π»ΡΠ½ΠΎΠΊΠ°ΠΌΠ΅Π½Π½Π°Ρ Π±ΠΎΠ»Π΅Π·Π½Ρ ΡΡΠ΅Π΄ΠΈ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠ³ΠΎ ΠΏΠ΅ΡΡΠΎΠ½Π°Π»Π° ΠΏΠΎΠ»ΠΈΠΊΠ»ΠΈΠ½ΠΈΠΊΠΈ: ΡΠ°ΠΊΡΠΎΡΡ ΡΠΈΡΠΊΠ° Π΅Π΅ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π² ΡΠ°Π·Π½ΡΡ ΠΏΡΠΎΡΠ΅ΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ Π³ΡΡΠΏΠΏΠ°Ρ
The aim. Identification of risk factors for gallstone disease (GDS) development between different professional groups of polyclinic medical staff. Material and methods. 75 medical staff members were divided into two groups (1st group: 30 doctors; 2nd group: 45 nurses). All of them were interviewed fo r information about profession, gender, age, waist circumference (WC), body mass index (BMI), smoking, arterial hypertension (AH), diabetes mellitus (DM) and hypercholesterolemia. In addition, women were interviewed about the number of pregnancies, ostmenopause and taking oral contraceptive pills (OCPs). To assess physical activity, a short international questionnaire on physical activity (IPAQ) was used. To identify the presence of professional burnout (PB) syndrome we applied the Maslach Burnout Inventory (MBI) questionnaire, adapted by N.Π. Vodopianova for medical staff. At the final stage, the data of abdominal organs ultrasound examination were analyzed based on the materials of medical staff outpatient records. Results. The GSD prevalence among doctors was 33.3%, and 24.4% among nurses. In both groups age, AH, weight, WC and BMI contribute to GSD formation. In the first group of individuals with GSD, the level of total cholesterol (TC) was significantly higher. In the same group extremely high PB, postmenopause (p <0.05) and hypodynamia (p <0.1) were much more common. In the second group, there were more DM cases and pregnancies (p <0.05). Conclusion. The medical staff of the clinic has a high GSD incidence, whose frequency depends not only on classical risk factors prevalence, but is also determined by profession peculiarities, characterized by high psychological stress and lower physical activity of physicians, compared to nurses.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΡΡΠ²ΠΈΡΡ ΡΠ°ΠΊΡΠΎΡΡ ΡΠΈΡΠΊΠ° ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΆΠ΅Π»ΡΠ½ΠΎΠΊΠ°ΠΌΠ΅Π½Π½ΠΎΠΉ Π±ΠΎΠ»Π΅Π·Π½ΠΈ (ΠΠΠ) ΡΡΠ΅Π΄ΠΈ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠ³ΠΎ ΠΏΠ΅ΡΡΠΎΠ½Π°Π»Π° ΠΏΠΎΠ»ΠΈΠΊΠ»ΠΈΠ½ΠΈΠΊΠΈ Π² ΡΠ°Π·Π½ΡΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΠ°Ρ
. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π ΠΏΠΎΠ»ΠΈΠΊΠ»ΠΈΠ½ΠΈΠΊΠ΅ Π±ΡΠ»ΠΎ ΠΎΡΠΌΠΎΡΡΠ΅Π½ΠΎ ΠΈ Π°Π½ΠΊΠ΅ΡΠΈΡΠΎΠ²Π°Π½ΠΎ 75 ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΡ
ΡΠΎΡΡΡΠ΄Π½ΠΈΠΊΠΎΠ² ΠΏΠΎΠ»ΠΈΠΊΠ»ΠΈΠ½ΠΈΠΊΠΈ, ΠΊΠΎΡΠΎΡΡΠ΅ Π±ΡΠ»ΠΈ ΡΠ°Π·Π΄Π΅Π»Π΅Π½Ρ Π½Π° Π΄Π²Π΅ Π³ΡΡΠΏΠΏΡ. Π 1-Ρ Π±ΡΠ»ΠΈ Π²ΠΊΠ»ΡΡΠ΅Π½Ρ 30 Π²ΡΠ°ΡΠ΅ΠΉ; Π²ΠΎ 2-Ρ - 45 ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΡ
ΡΠ΅ΡΡΠ΅Ρ. ΠΠ½Π°Π»ΠΈΠ·Ρ ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π°Π»ΠΈΡΡ ΡΠ°ΠΊΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ, ΠΊΠ°ΠΊ ΠΏΡΠΎΡΠ΅ΡΡΠΈΡ, ΠΏΠΎΠ», Π²ΠΎΠ·ΡΠ°ΡΡ, ΠΎΠ±ΡΠ΅ΠΌ ΡΠ°Π»ΠΈΠΈ (ΠΠ’), ΠΈΠ½Π΄Π΅ΠΊΡ ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π° (ΠΠΠ’), ΠΊΡΡΠ΅Π½ΠΈΠ΅, Π½Π°Π»ΠΈΡΠΈΠ΅ Π°ΡΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π³ΠΈΠΏΠ΅ΡΡΠ΅Π½Π·ΠΈΠΈ (ΠΠ), ΡΠ°Ρ
Π°ΡΠ½ΠΎΠ³ΠΎ Π΄ΠΈΠ°Π±Π΅ΡΠ° (Π‘Π), Π³ΠΈΠΏΠ΅ΡΡ
ΠΎΠ»Π΅ΡΡΠ΅ΡΠΈΠ½Π΅ΠΌΠΈΠΈ, ΠΏΠΎΡΡΠΌΠ΅Π½ΠΎΠΏΠ°ΡΠ·Ρ; Ρ ΠΆΠ΅Π½ΡΠΈΠ½ β ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ Π±Π΅ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΠΈ ΠΏΡΠΈΠ΅ΠΌ ΠΎΡΠ°Π»ΡΠ½ΡΡ
ΠΊΠΎΠ½ΡΡΠ°ΡΠ΅ΠΏΡΠΈΠ²ΠΎΠ² (ΠΠ). ΠΠ»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΡΠΈΠΌΠ΅Π½ΡΠ»ΠΈ ΠΊΡΠ°ΡΠΊΠΈΠΉ ΠΌΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½ΡΠΉ ΠΎΠΏΡΠΎΡΠ½ΠΈΠΊ ΠΏΠΎ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ (IPAQ). ΠΠ»Ρ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΡΠΈΠΌΠΏΡΠΎΠΌΠΎΠ² ΠΏΡΠΎΡΠ΅ΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π²ΡΠ³ΠΎΡΠ°Π½ΠΈΡ (ΠΠ) ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΎΠΏΡΠΎΡΠ½ΠΈΠΊ Maslach Burnout Inventory (MBI), Π°Π΄Π°ΠΏΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΉ Π.Π. ΠΠΎΠ΄ΠΎΠΏΡΡΠ½ΠΎΠ²ΠΎΠΉ Π΄Π»Ρ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΡ
ΡΠΎΡΡΡΠ΄Π½ΠΈΠΊΠΎΠ². ΠΠ° ΠΊΠΎΠ½Π΅ΡΠ½ΠΎΠΌ ΡΡΠ°ΠΏΠ΅ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π»ΠΈΡΡ Π΄Π°Π½Π½ΡΠ΅ ΡΠ»ΡΡΡΠ°Π·Π²ΡΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΎΡΠ³Π°Π½ΠΎΠ² Π±ΡΡΡΠ½ΠΎΠΉ ΠΏΠΎΠ»ΠΎΡΡΠΈ ΠΏΠΎ Π°ΠΌΠ±ΡΠ»Π°ΡΠΎΡΠ½ΡΠΌ ΠΊΠ°ΡΡΠ°ΠΌ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΡ
ΡΠ°Π±ΠΎΡΠ½ΠΈΠΊΠΎΠ². Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π Π°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΡ ΠΠΠ ΡΡΠ΅Π΄ΠΈ Π²ΡΠ°ΡΠ΅ΠΉ ΡΠΎΡΡΠ°Π²ΠΈΠ»Π° 33,3%, ΠΈ 24,4% ΡΡΠ΅Π΄ΠΈ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΡ
ΡΠ΅ΡΡΠ΅Ρ. Π ΠΎΠ±Π΅ΠΈΡ
Π³ΡΡΠΏΠΏΠ°Ρ
Π½Π° ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠΠ Π²Π»ΠΈΡΡΡ Π²ΠΎΠ·ΡΠ°ΡΡ, Π½Π°Π»ΠΈΡΠΈΠ΅ ΠΠ, Π²Π΅Ρ, ΠΠ’, ΠΠΠ’. Π ΠΏΠ΅ΡΠ²ΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅ Π»ΠΈΡ Ρ ΠΠΠ ΡΡΠΎΠ²Π΅Π½Ρ ΠΎΠ±ΡΠ΅Π³ΠΎ Ρ
ΠΎΠ»Π΅ΡΡΠ΅ΡΠΈΠ½Π° Π±ΡΠ» Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π²ΡΡΠ΅ (ΠΠ₯). Π ΡΡΠΎΠΉ ΠΆΠ΅ Π³ΡΡΠΏΠΏΠ΅ ΠΠ ΠΊΡΠ°ΠΉΠ½Π΅ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ, ΠΏΠΎΡΡΠΌΠ΅Π½ΠΎΠΏΠ°ΡΠ·Π° (p<0,05) ΠΈ Π³ΠΈΠΏΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡ (p<0,1) Π²ΡΡΡΠ΅ΡΠ°Π»ΠΈΡΡ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΠ°ΡΠ΅. Π Π³ΡΡΠΏΠΏΠ΅ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΡ
ΡΠ΅ΡΡΠ΅Ρ Ρ ΠΠΠ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΎΡΡ ΠΏΡΠ΅ΠΎΠ±Π»Π°Π΄Π°Π½ΠΈΠ΅ Π»ΠΈΡ Ρ Π‘Π ΠΈ Π±ΠΎΠ»ΡΡΠ΅Π΅ ΡΠΈΡΠ»ΠΎ Π±Π΅ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΡΡΠ΅ΠΉ (p<0,05). ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π£ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠ³ΠΎ ΠΏΠ΅ΡΡΠΎΠ½Π°Π»Π° ΠΏΠΎΠ»ΠΈΠΊΠ»ΠΈΠ½ΠΈΠΊΠΈ ΠΈΠΌΠ΅Π΅Ρ ΠΌΠ΅ΡΡΠΎ Π²ΡΡΠΎΠΊΠ°Ρ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΡ ΠΠΠ, ΡΠ°ΡΡΠΎΡΠ° ΠΊΠΎΡΠΎΡΠΎΠΉ Π·Π°Π²ΠΈΡΠΈΡ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ ΠΎΡ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΠΈ ΠΊΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ² ΡΠΈΡΠΊΠ°, Π½ΠΎ ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΡΡΡ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΡΠΌΠΈ ΠΏΡΠΎΡΠ΅ΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ, Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΠ΅ΠΉΡΡ Π²ΡΡΠΎΠΊΠΎΠΉ ΠΏΡΠΈΡ
ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π°Π³ΡΡΠ·ΠΊΠΎΠΉ ΠΈ Π±ΠΎΠ»Π΅Π΅ Π½ΠΈΠ·ΠΊΠΎΠΉ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ Π²ΡΠ°ΡΠ΅Π±Π½ΠΎΠ³ΠΎ ΠΏΠ΅ΡΡΠΎΠ½Π°Π»Π°, ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΠΌΠΈ ΡΠ΅ΡΡΡΠ°ΠΌΠΈ
ΠΠ°ΡΠ°ΠΌΠ΅ΡΡΡ ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ ΠΊΠ°ΠΊ ΠΌΠ°ΡΠΊΠ΅ΡΡ Π½Π΅ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π΄Π°ΠΏΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ° ΠΏΡΠΈ ΠΎΡΡΡΡΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΡΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΡ Ρ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ ΡΠΎΠ½Π·ΠΈΠ»Π»ΠΈΡΠ°
Evaluation of nonspecific adaptive response of the body in children with acute infectious diseases associated with tonsillitis syndrome was the aim of this research. This prospective study included clinical, anamnestic and laboratory examination of children with acute infectious diseases with tonsillitis syndrome. A systemic multiple factor analysis was conducted (significance level Ρ<0.05). The evaluation of peripheral blood parameters (specific gravity of lymphocytes and indices of reactive protective potential (RPP) - specific immune lymphocytic-monocytic parameter (SILMP) and coefficient of phagocytic defense (CPD)) gives the possibility to determine the condition of nonspecific adaptation in children with acute infectious diseases associated with tonsillitis syndrome. Children with tonsillitis syndrome show significant increase of integral RPP parameters, i.e. decrease of RPP, which is more pronounced on discharge (on admission - in 57% of children, on discharge - in 87%). The most unfavourable initial nonspecific adaptative body response (NABR) (according to the percentage of lymphocytes) is the reaction of increased activation, as it is associated with the transition to the overactivation by the time of discharge (37% of children). Systemic multiple factor analysis determined which peripheral blood parameters have more influence on childrenβs adaptation during the course of infectious disease: RPP indices showed high influence coefficients in all diseases (CPD was the highest in bacterial infection - Π i 198.3; SILMP was the highest in viral infection - Π i 147.81; in mixed infection CPD and SILMP were roughly the same - Π i β 107.25 and Π i - 78.11, respectively), which proves the feasibility of RPP evaluation for prognostic purposes in the treatment of this category of patients.Π¦Π΅Π»ΡΡ Π½Π°ΡΡΠΎΡΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²ΠΈΠ»Π°ΡΡ ΠΎΡΠ΅Π½ΠΊΠ° Π½Π΅ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°Π΄Π°ΠΏΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ° Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ ΠΎΡΡΡΡΠΌΠΈ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΡΠΌΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΠΌΠΈ, Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ Ρ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ ΡΠΎΠ½Π·ΠΈΠ»Π»ΠΈΡΠ°. ΠΠ°Π½Π½ΠΎΠ΅ ΠΏΡΠΎΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΠΊΠ»ΡΡΠ°Π»ΠΎ ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-Π°Π½Π°ΠΌΠ½Π΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΠΎΠ΅ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π΄Π΅ΡΠ΅ΠΉ Ρ ΠΎΡΡΡΡΠΌΠΈ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΡΠΌΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΠΌΠΈ Ρ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ ΡΠΎΠ½Π·ΠΈΠ»Π»ΠΈΡΠ°. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΠΈΡΡΠ΅ΠΌΠ½ΡΠΉ ΠΌΠ½ΠΎΠ³ΠΎΡΠ°ΠΊΡΠΎΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· (ΡΡΠΎΠ²Π΅Π½Ρ Π·Π½Π°ΡΠΈΠΌΠΎΡΡΠΈ Ρ<0,05). ΠΡΠ΅Π½ΠΊΠ° ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ (ΡΠ΄Π΅Π»ΡΠ½ΠΎΠ³ΠΎ Π²Π΅ΡΠ° Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΈ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΡΠ΅Π°ΠΊΡΠΈΠ²Π½ΠΎ-ΠΏΡΠΎΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΉ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π» (Π ΠΠ) - ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΎ Π»ΠΈΠΌΡΠΎΡΠΈΡΠ°ΡΠ½ΠΎ-ΠΌΠΎΠ½ΠΎΡΠΈΡΠ°ΡΠ½ΠΎΠ³ΠΎ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠ° (ΠΠΠΠ) ΠΈ ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½ΡΠ° ΡΠ°Π³ΠΎΡΠΈΡΠ°ΡΠ½ΠΎΠΉ Π·Π°ΡΠΈΡΡ (ΠΠ€Π) Π΄Π°Π΅Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅ Π½Π΅ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°Π΄Π°ΠΏΡΠ°ΡΠΈΠΈ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ ΠΎΡΡΡΡΠΌΠΈ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΡΠΌΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΠΌΠΈ, Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ Ρ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ ΡΠΎΠ½Π·ΠΈΠ»Π»ΠΈΡΠ°. Π£ Π΄Π΅ΡΠ΅ΠΉ Ρ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ ΡΠΎΠ½Π·ΠΈΠ»Π»ΠΈΡΠ° ΠΎΡΠΌΠ΅ΡΠ°Π΅ΡΡΡ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎΠ΅ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΠΈΠ½ΡΠ΅Π³ΡΠ°Π»ΡΠ½ΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ Π ΠΠ, Ρ. Π΅. ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ Π ΠΠ, ΠΊΠΎΡΠΎΡΠΎΠ΅ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½ΠΎ ΠΏΡΠΈ Π²ΡΠΏΠΈΡΠΊΠ΅ (ΠΏΡΠΈ ΠΏΠΎΡΡΡΠΏΠ»Π΅Π½ΠΈΠΈ - Ρ 57% Π΄Π΅ΡΠ΅ΠΉ, ΠΏΡΠΈ Π²ΡΠΏΠΈΡΠΊΠ΅ - Ρ 87%). ΠΠ°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π½Π΅Π±Π»Π°Π³ΠΎΠΏΡΠΈΡΡΠ½ΠΎΠΉ ΠΈΡΡ
ΠΎΠ΄Π½ΠΎΠΉ Π½Π΅ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°Π΄Π°ΠΏΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠ΅ΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ° (ΠΏΠΎ ΠΏΡΠΎΡΠ΅Π½ΡΡ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ²) ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ΅Π°ΠΊΡΠΈΡ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ, ΡΠ°ΠΊ ΠΊΠ°ΠΊ ΠΎΠ½Π° ΡΠ²ΡΠ·Π°Π½Π° Ρ ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄ΠΎΠΌ ΠΊ Π³ΠΈΠΏΠ΅ΡΠ°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ ΠΊ ΠΌΠΎΠΌΠ΅Π½ΡΡ Π²ΡΠΏΠΈΡΠΊΠΈ (37% Π΄Π΅ΡΠ΅ΠΉ). Π‘ΠΈΡΡΠ΅ΠΌΠ½ΡΠΉ ΠΌΠ½ΠΎΠ³ΠΎΡΠ°ΠΊΡΠΎΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΠ», ΠΊΠ°ΠΊΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ ΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡ Π±ΠΎΠ»ΡΡΠ΅Π΅ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½Π° Π°Π΄Π°ΠΏΡΠ°ΡΠΈΡ Π΄Π΅ΡΠ΅ΠΉ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ: ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ Π ΠΠ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ Π²ΡΡΠΎΠΊΠΈΠ΅ ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½ΡΡ Π²Π»ΠΈΡΠ½ΠΈΡ ΠΏΡΠΈ Π²ΡΠ΅Ρ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΡ
, ΡΡΠΎ Π΄ΠΎΠΊΠ°Π·ΡΠ²Π°Π΅Ρ ΡΠ΅Π»Π΅ΡΠΎΠΎΠ±ΡΠ°Π·Π½ΠΎΡΡΡ ΠΎΡΠ΅Π½ΠΊΠΈ Π ΠΠ Π² ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ΅Π»ΡΡ
ΠΏΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΠΈ Π΄Π°Π½Π½ΠΎΠΉ ΠΊΠ°ΡΠ΅Π³ΠΎΡΠΈΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
Changes of photosynthesis and carbon metabolism in typha angustifolia L grown in conditions of nitrate nitrogen overload
Nitrates may induce alterations in NO-signaling system and change photosynthesis in plants. Significant reduction of 14CO 2 fixation was noted at concentration of 3.96 mM NaNO 3 in an aquatic macrophyte (Typha angustifolia L.). Assimilation of 14CO 2 seven days after the introduction of nitrates did not differ between control and experimental samples. There were changes in distribution of 14C among products of 4CO 2 fixation 4 h after NaNO 3 addition, resulting in increased sugar radioactivity in experimental plants. It was suggested that the observed changes may have regulatory importance. Β© 2012 by Acta Botanica Croatica, the Faculty of Science, University of Zagreb
Altered brain energetics induces mitochondrial fission arrest in Alzheimer's Disease.
Altered brain metabolism is associated with progression of Alzheimer's Disease (AD). Mitochondria respond to bioenergetic changes by continuous fission and fusion. To account for three dimensional architecture of the brain tissue and organelles, we applied 3-dimensional electron microscopy (3D EM) reconstruction to visualize mitochondrial structure in the brain tissue from patients and mouse models of AD. We identified a previously unknown mitochondrial fission arrest phenotype that results in elongated interconnected organelles, "mitochondria-on-a-string" (MOAS). Our data suggest that MOAS formation may occur at the final stages of fission process and was not associated with altered translocation of activated dynamin related protein 1 (Drp1) to mitochondria but with reduced GTPase activity. Since MOAS formation was also observed in the brain tissue of wild-type mice in response to hypoxia or during chronological aging, fission arrest may represent fundamental compensatory adaptation to bioenergetic stress providing protection against mitophagy that may preserve residual mitochondrial function. The discovery of novel mitochondrial phenotype that occurs in the brain tissue in response to energetic stress accurately detected only using 3D EM reconstruction argues for a major role of mitochondrial dynamics in regulating neuronal survival
Extracellular vesicles derived from Acholeplasma laidlawii PG8
Extracellular vesicle production is believed to be a ubiquitous process in bacteria, but the data on such a process in Mollicutes are absent. We report the isolation of ultramicroforms - extracellular vesicles from supernatants of Acholeplasma laidlawii PG8 (ubiquitous mycoplasma; the main contaminant of cell culture). Considering sizes, morphology, and ultrastructural organization, the ultramicroforms of A. laidlawii PG8 are similar to membrane vesicles of Gram-positive and Gram-negative bacteria. We demonstrate that A. laidlawii PG8 vesicles contain genetic material and proteins, and are mutagenic to lymphocytes of human peripheral blood. We show that Mycoplasma gallisepticum S6, the other mycoplasma, also produce similar structures, which suggests that shedding of the vesicles might be the common phenomenon in Mollicutes. We found that the action of stress conditions results in the intensive formation of ultramicroforms in mycoplasmas. The role of vesicular formation in mycoplasmas remains to be studied. Β©2011 with author. Published by TheScientificWorld
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