6 research outputs found
Current Knowledge on Selenium Biofortification to Improve the Nutraceutical Profile of Food: A Comprehensive Review
Selenium (Se) is an important micronutrient for living organisms, since it is involved in several physiological and metabolic processes. Se intake in humans is often low and very seldom excessive, and its bioavailability depends also on its chemical form, with organic Se as the most available after ingestion. The main dietary source of Se for humans is represented by plants, since many species are able to metabolize and accumulate organic Se in edible parts to be consumed directly (leaves, flowers, fruits, seeds, and sprouts) or after processing (oil, wine, etc.). Countless studies have recently investigated the Se biofortification of plants to produce Se-enriched foods and elicit the production of secondary metabolites, which may benefit human health when incorporated into the diet. Moreover, feeding animals Se-rich diets may provide Se-enriched meat. This work reviews the most recent literature on the nutraceutical profile of Se-enriched foods from plant and animal sources.Fil: D'Amato, Roberto. Università di Perugia; ItaliaFil: Regni, Luca. Università di Perugia; ItaliaFil: Falcinelli, Beatrice. Università di Perugia; ItaliaFil: Mattioli, Simona. Università di Perugia; ItaliaFil: Benincasa, Paolo. Università di Perugia; ItaliaFil: Dal Bosco, Alessandro. Università di Perugia; ItaliaFil: Pacheco, Pablo Hugo. Consejo Nacional de Investigaciones CientÃficas y Técnicas. Centro CientÃfico Tecnológico Conicet - San Luis. Instituto de QuÃmica de San Luis. Universidad Nacional de San Luis. Facultad de QuÃmica, BioquÃmica y Farmacia. Instituto de QuÃmica de San Luis; ArgentinaFil: Proietti, Primo. Università di Perugia; ItaliaFil: Troni, Elisabetta. Università di Perugia; ItaliaFil: Santi, Claudio. Università di Perugia; ItaliaFil: Businelli, Daniela. Università di Perugia; Itali
In Vitro Evaluation of the Inhibitory Activity of Different Selenium Chemical Forms on the Growth of a Fusarium proliferatum Strain Isolated from Rice Seedlings
In this study, the in vitro effects of different Se concentrations (5, 10, 15, 20, and 100 mg kg−1) from different Se forms (sodium selenite, sodium selenate, selenomethionine, and selenocystine) on the development of a Fusarium proliferatum strain isolated from rice were investigated. A concentration-dependent effect was detected. Se reduced fungal growth starting from 10 mg kg−1 and increasing the concentration (15, 20, and 100 mg kg−1) enhanced the inhibitory effect. Se bioactivity was also chemical form dependent. Selenocystine was found to be the most effective at the lowest concentration (5 mg kg−1). Complete growth inhibition was observed at 20 mg kg−1 of Se from selenite, selenomethionine, and selenocystine. Se speciation analysis revealed that fungus was able to change the Se speciation when the lowest Se concentration was applied. Scanning Electron Microscopy showed an alteration of the fungal morphology induced by Se. Considering that the inorganic forms have a higher solubility in water and are cheaper than organic forms, 20 mg kg−1 of Se from selenite can be suggested as the best combination suitable to inhibit F. proliferatum strain. The addition of low concentrations of Se from selenite to conventional fungicides may be a promising alternative approach for the control of Fusarium species
Small is Beautiful: The Unusual Transformation of Nanocrystalline Layered α‑Zirconium Phosphate into a New 3D Structure
Nanosized
α-zirconium phosphate, α-ZrP, undergoes a phase transition
at 120 °C, which is not observed with microcrystalline α-ZrP
in the same conditions, and which leads to a new 3D phase. The new
compound, with formula ZrÂ(HPO<sub>4</sub>)<sub>2</sub> (τ′-ZrP),
consists of cubelike nanoparticles and has a tetragonal unit cell
(space group <i>P</i>4<sub>3</sub>2<sub>1</sub>2, <i>a</i> = 7.955 Ã…, <i>c</i> = 10.744 Ã…). The
structure of τ′-ZrP is in close relationship with that
of the already known Ï„-ZrP. Both structures are made of packed
chains of eight-membered rings, composed of Zr atoms connected to
bridging HPO<sub>4</sub> groups. The main difference between the two
structures concerns the different orientation of the uncoordinated
P–OH groups, pointing into the channels. The in situ XRPD analysis
on nanosized α-ZrP, performed at 120 °C as a function of
time, provided information about the kinetics of the formation of
τ′-ZrP, showing that the α-ZrP phase is directly
transformed into τ′-ZrP. Moreover, τ′-ZrP
is converted into α-ZrP at room temperature in the presence
of water vapor. It was proved that the free phosphoric acid, which
is originally present in small amounts in nanosized α-ZrP and
τ′-ZrP, is necessary for the interconversion between
the two phases. As a matter of fact, the removal of phosphoric acid,
by washing α-ZrP and τ′-ZrP with anhydrous ethanol,
inhibits the above conversion
A nanoscale interface promoting molecular and functional differentiation of neural cells
Potassium channels and aquaporins expressed by astrocytes are key players in the maintenance of cerebral homeostasis and in brain pathophysiologies. One major challenge in the study of astrocyte membrane channels in vitro, is that their expression pattern does not resemble the one observed in vivo. Nanostructured interfaces represent a significant resource to control the cellular behaviour and functionalities at micro and nanoscale as well as to generate novel and more reliable models to study astrocytes in vitro. However, the potential of nanotechnologies in the manipulation of astrocytes ion channels and aquaporins has never been previously reported. Hydrotalcite-like compounds (HTlc) are layered materials with increasing potential as biocompatible nanoscale interface. Here, we evaluate the effect of the interaction of HTlc nanoparticles films with primary rat neocortical astrocytes. We show that HTlc films are biocompatible and do not promote gliotic reaction, while favouring astrocytes differentiation by induction of F-actin fibre alignment and vinculin polarization. Western Blot, Immunofluorescence and patch-clamp revealed that differentiation was accompanied by molecular and functional up-regulation of both inward rectifying potassium channel Kir 4.1 and aquaporin 4, AQP4. The reported results pave the way to engineering novel in vitro models to study astrocytes in a in vivo like condition
In vitro Evaluation of the Inhibitory Activity of Different Selenium Chemical Forms on a Fusarium proliferatum Strain Isolated from Rice Seedlings
In this study, the in vitro effects of different Se concentrations (5, 10, 15, 20, and 100 mg kg 121) from different Se forms (sodium selenite, sodium selenate, selenomethionine, and selenocystine) on the development of a Fusarium proliferatum strain isolated from rice were investigated. A concentration-dependent effect was detected. Se reduced fungal growth starting from 10 mg kg 121 and increasing the concentration (15, 20, and 100 mg kg 121) enhanced the inhibitory effect. Se bioactivity was also chemical form dependent. Selenocystine was found to be the most effective at the lowest concentration (5 mg kg 121). Complete growth inhibition was observed at 20 mg kg 121 of Se from selenite, selenomethionine, and selenocystine. Se speciation analysis revealed that fungus was able to change the Se speciation when the lowest Se concentration was applied. Scanning Electron Microscopy showed an alteration of the fungal morphology induced by Se. Considering that the inorganic forms have a higher solubility in water and are cheaper than organic forms, 20 mg kg 121 of Se from selenite can be suggested as the best combination suitable to inhibit F. proliferatum strain. The addition of low concentrations of Se from selenite to conventional fungicides may be a promising alternative approach for the control of Fusarium species