Avaliação participativa de adubos verdes em assentamentos de reforma agrária de Corumbá, MS: resultados preliminares.

O texto relata o resultados preliminares do processo de avaliação participativa de Unidades de Observação de oito espécies de adubos verdes implantadas em 03 assentamentos de reforma agrária, no Município de Corumbá, MS. Os experimentos foram conduzidos no delineamento de blocos ao acaso, onde cada bloco foi instalado em uma propriedade diferente juntamente com um grupo de agricultores. Além da avaliação estatística convencional foi feita uma avaliação qualitativa por técnicos e agricultores mediante um questionário com a definição de 13 critérios. O último critério referiu-se a nota geral para cada espécie, numa escala de 1 a 10, cujos resultados serão aqui discutidos. Com pequenas diferenças a avaliação dos técnicos foi coincidente com a dos agricultores. This work presents the preliminary results of a participative evaluation of eight green manure species in three land reform settlements, in Corumbá, MS. The experiment was performed in randomized blocs design, with each bloc installed in a different farm, together with a group of farmers. Beside a quantitative statistical evaluation, I was made a qualitative evaluation by technicians and farmers by a list of 13 criteria. The last criteria was a general prefernce note for each specie, with a scale varies from 1 to 10, and which results are discussed in this work. With little differences the technicians preferences were coincident with there from the farmers

Violacein Extracted from Chromobacterium violaceum Inhibits Plasmodium Growth in Vitro and in Vivo

Violacein is a violet pigment extracted from the gram-negative bacterium Chromobacterium violaceum. It presents bactericidal, tumoricidal, trypanocidal, and antileishmanial activities. We show that micromolar concentrations efficiently killed chloroquine-sensitive and -resistant Plasmodium falciparum strains in vitro; inhibited parasitemia in vivo, even after parasite establishment; and protected Plasmodium chabaudi chabaudi-infected mice from a lethal challenge.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Campinas, UNICAMP, Dept Parasitol, Inst Biol, BR-13083970 Campinas, SP, BrazilUniv Estadual Campinas, UNICAMP, Dept Microbiol & Imunol, Inst Biol, BR-13083970 Campinas, SP, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Bioquim, BR-04044020 São Paulo, BrazilCEPEM, IPEPATRO, BR-78900970 Porto Velho, RO, BrazilUniv São Paulo, Dept Parasitol, ICB2, São Paulo, BrazilUniv Estadual Campinas, Dept Fisiol & Biofis, Inst Biol, BR-13083970 Campinas, SP, BrazilUniv Estadual Campinas, Lab Quim Biol, Inst Quim, BR-13083970 Campinas, SP, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Bioquim, BR-04044020 São Paulo, BrazilFAPESP: 2004/00638-6CNPq: 470587/2006-7Web of Scienc

ESC core curriculum for the general cardiologist (2013)

[No abstract available

Ecologização da pecuária familiar na Serra do Sudeste.

Histórico; Local de execução e perfil da população; Identificação dos elementos e interações; Modelo sistêmico / Sistemas de produção.bitstream/item/63681/1/DT98.pd

Cellular and tissue effects induced by Photogem® and red LED in photodynamic therapy

In order to consider the photodynamic therapy (PDT) as a clinical treatment for candidosis, it is necessary to know its cytotoxic effect on normal cells and tissues. Therefore, this study evaluated the toxicity of PDT with Photogem® associated with red light-emitting diode (LED) on L929 and MDPC-23 cell cultures and healthy rat palatal mucosa. In the in vitro experiment, the cells (30000 cells/cm2) were seeded in 24-well plates for 48 h, incubated with Photogem® (50, 100, or 150 mg/l) and either irradiated or not with a red LED source (630 ± 3 nm; 75 or 100 J/cm2; 22 mW/cm2). Cell metabolism was evaluated by the MTT assay (ANOVA and Dunnet’s post hoc tests; p < 0.05) and cell morphology was examined by scanning electron microscopy. In the in vivo evaluation, Photogem® (500 mg/l) was applied to the palatal mucosa of Wistar rats during 30 min and exposed to red LED (630 nm) during 20 min (306 J/cm2). The palatal mucosa was photographed for macroscopic analysis at 0, 1, 3, and 7 days posttreatment and subjected to histological analysis after sacrifice of the rats. For both cell lines, there was a statistically significant decrease of the mitochondrial activity (90-97%) for all Photogem® concentrations associated with red LED regardless of the energy density. However, in the in vivo evaluation, the PDT-treated groups presented intact mucosa with normal characteristics both macroscopically and histologically. From these results, it may be concluded that the association of Photogem® and red LED caused severe toxic effects on normal cell cultures, characterized by the reduction of mitochondrial activity and morphological alterations, but did not cause damage to the rat palatal mucosa in vivo.FAPESP (07/04376-4

Volumetric laser endomicroscopy and its application to Barrett's esophagus: results from a 1,000 patient registry.

Volumetric laser endomicroscopy (VLE) uses optical coherence tomography (OCT) for real-time, microscopic cross-sectional imaging. A US-based multi-center registry was constructed to prospectively collect data on patients undergoing upper endoscopy during which a VLE scan was performed. The objective of this registry was to determine usage patterns of VLE in clinical practice and to estimate quantitative and qualitative performance metrics as they are applied to Barrett's esophagus (BE) management. All procedures utilized the NvisionVLE Imaging System (NinePoint Medical, Bedford, MA) which was used by investigators to identify the tissue types present, along with focal areas of concern. Following the VLE procedure, investigators were asked to answer six key questions regarding how VLE impacted each case. Statistical analyses including neoplasia diagnostic yield improvement using VLE was performed. One thousand patients were enrolled across 18 US trial sites from August 2014 through April 2016. In patients with previously diagnosed or suspected BE (894/1000), investigators used VLE and identified areas of concern not seen on white light endoscopy (WLE) in 59% of the procedures. VLE imaging also guided tissue acquisition and treatment in 71% and 54% of procedures, respectively. VLE as an adjunct modality improved the neoplasia diagnostic yield by 55% beyond the standard of care practice. In patients with no prior history of therapy, and without visual findings from other technologies, VLE-guided tissue acquisition increased neoplasia detection over random biopsies by 700%. Registry investigators reported that VLE improved the BE management process when used as an adjunct tissue acquisition and treatment guidance tool. The ability of VLE to image large segments of the esophagus with microscopic cross-sectional detail may provide additional benefits including higher yield biopsies and more efficient tissue acquisition. Clinicaltrials.gov NCT02215291

Hematologia e bioquímica sérica de bovinos curraleiro pé duro infectados por Babesia spp. e Leptospira spp.

A Babesia spp. e a Leptospira spp. são importantes enfermidades em sistemas de produção pecuária de países tropicais como o Brasil podendo causar alterações laboratoriais em animais clinicamente sadios. Esse trabalho teve por objetivo avaliar os títulos de anticorpos anti-Babesia bovis, anti-Babesia bigemina e anti-Leptospira interrogans, em bovinos da raça Curraleiro Pé Duro e correlacioná-los às características hematológicas e da bioquímica sérica em animais sadios submetidos à infecção natural. Em 2003 foram amostrados dois rebanhos, localizados no Estado de Goiás e Tocantins e avaliadas 282 amostras para detecção de anticorpos anti-B. bovis e anti- B. bigemina e 236 amostras para detecção de anticorpos anti-Leptospira spp. Foram realizados, hemograma, determinação da atividade sérica de Aspartato Amino Transferase (AST), Alanina Amino Transferase (ALP) e Gama Glutamil Transferase (GGT) e a quantificação no soro da proteína total (PT), albumina, colesterol, uréia, creatinina e bilirrubina, de todos os animais amostrados. Obteve-se 92,90% (n=262) de positividade para B. bovis, 85,46% (n=241) para B. bigemina e 33,05% (n=78) para L. interrogans. Não houve qualquer alteração nos valores médios hematológicos e da bioquímica sérica em relação aos valores de referência. Os resultados não mostraram nenhuma correlação entre títulos de anticorpos anti-Leptospiras e as variáveis laboratoriais avaliadas. O nível de anticorpos específicos para Babesia spp. apresentou correlação positiva com os valores de hemácias, hemoglobina, leucócitos totais e linfócitos, além de AST, ALP, bilirrubina direta (BD), colesterol e creatinina. Concluiu-se que as correlações observadas entre os anticorpos anti-Babesia sp. estão relacionados principalmente ao estímulo do sistema imunológico, funções hepática e renal. Porém, como todos os valores encontrados estão dentro do intervalo de referência, concluiu-se que não houve manifestação patológica decorrente da infecção ou de maiores títulos de imunoglobulinas.bitstream/item/171695/1/Juliano-BP-Curraleiro-final.pd