Sistema de gestão da qualidade e ambiental na Embrapa Arroz e Feijão.

O presente documento relata o histórico dessa mudança de filosofia institucional e mostra a interface existente entre a Gestão da Qualidade e Ambiental, para identificar e desenvolver estratégias para aplicação de modelos de planejamento integrado, que ofereçam subsídios para a sustentabilidade da empresa.bitstream/CNPAF/26654/1/doc_215.pd

Evaluating structural differences in cortical bone tissue after demineralization and calcination

Although the best results in bone grafting have been achieved with autogeneuos bone tissue, allografts and xenografts have been widely used either in mineralized, demineralized, or calcined forms. Demineralized bone has been proven to stimulate new bone formation by exposing, proteins and growth factors necessary for osteoinduction. On the other hand, calcined bone offers a natural architectural mineralized matrix, not present in synthetic apatite materials, as well as an excellent source of calcium. Despite the extensive use and importance of these materials, systematic works regarding their characterization are relatively scarce

Circular economy applied to eco-design of cement furniture loaded with ceramic and glass waste

Foram desenvolvidos novos materiais de cimento carregado com resíduos de vidro e cerâmica. Estes novos materiais apresentam-se com alto potencial de concorrerem vantajosamente em termos ecológicos e económicos com pedras ornamentais naturais e pedras sintéticas. Foram projetados novos produtos de mobiliário e seus acessórios que integram ou serão totalmente produzidos nos novos materiais. Este estudo perspetiva a possibilidade de dar resposta positiva aos desafios e às oportunidades para o desenvolvimento da economia circular no setor da cerâmica e do vidro nomeadamente a partir da valorização dos resíduos destas indústrias a partir do seu elevado potencial de cor. O potencial que a cor destes resíduos pode ter ao nível da valorização e diferenciação de produtos de mobiliário e seus acessórios demonstra a possibilidade de simbioses industriais entre os setores da cerâmica e do vidro e da produção de artefactos em cimento.New cement materials loaded with glass and ceramic waste have been developed. These new materials have a high potential to compete advantageously in ecological and economic terms with natural ornamental stones and synthetic stones. New furniture products and acessories have been designed that integrate or will be produced entirely in the new materials. This study envisages the possibility of giving a positive answer to the challenges and opportunities for the development of the circular economy in the ceramics and glass sector, namely through the valorisation of the residues of these industries based on their high color potential. The potential that the color of these residues can have in terms of the valuation and differentiation of furniture products and their accessories demonstrates the possibility of industrial symbiosis between the sectors of ceramics and glass and the production of cement artefacts

Synthesis of CdS and CdSe nanocrystallites using a novel single-molecule precursors approach

The synthesis of CdS and CdSe nanocrystallites using the thermolysis of several dithioor diselenocarbamato complexes of cadmium in trioctylphosphine oxide (TOPO) is reported. The nanodispersed materials obtained show quantum size effects in their optical spectra and exhibit near band-edge luminescence. The influence of experimental parameters on the properties of the nanocrystallites is discussed. HRTEM images of these materials show well-defined, crystalline nanosized particles. Standard size fractionation procedures can be performed in order to narrow the size dispersion of the samples. The TOPO-capped CdS and CdSe nanocrystallites and simple organic bridging ligands, such as 2,2¢-bipyrimidine, are used as the starting materials for the preparation of novel nanocomposites. The optical properties shown by these new nanocomposites are compared with those of the starting nanodispersed materials

Análise proteômica de isolados de Leishmania (Leishmania) chagasi sensíveis e resistente à Miltefosina.

Leishmaniose visceral (LV) é uma doença sistêmica, fatal se não tratada, causada por parasitas protozoários do gênero Leishmania complexo donovani, o qual abriga a espécie L. chagasi. O tratamento da LV conta com poucas opções terapêuticas, incluindo os antimoniais petnavalentes, anfotericina B e a miltefosina. A miltefosina foi recentemente aprovada como a primeira droga de administração oral para o tratamento da LV. Os mecanismos de resistência à miltefosina estão sendo elucidados em linhagens experimentais de Leishmania spp. resistentes a esta droga. Entretanto, os mecnaismos de resistência à miltefosina em isolados clínicos de Leishmania são poucos conhecidos. Neste estudo foi utilizada a técnica de eletroforese bidimensional acoplada à espectrometria de massas com o objetivo de destacar e identificar proteínas que são diferencialmente experessas entre formas promastigotas de isolados clínicos de L. chagasi sensíveis (S: S1 e S2) e resistentes (R: R1 e R2) Pa miltefosina, obtidos de pacientes com LV que participaram de um estudo clínico realizado no Brasil para avaliar a eficácia dessa droga. Os perfis protéicos obtidos dos isolados clínicos apresentaram em média 459 "spots" correpondendo a 5,7% dos produtos gênicos prditos para Leishmania spp. A análise comparativa entre os perfis protéicos dos isolados clínicos S e R permitiu a detecção de 80 "spots" diferencialmente expressos. Desses 18 "spots" foram encontrados exclusivamente no perfil do grupo S e apenas 1 no grupo R, enquanto 48 "spots" apresentaram diferença quantitativa na expressão protéica entre os grupos. Os demais "spots" foram encontrados em um único isolado (7 em S1, 3 em S2 e 2 em R1) ou em três isolados simultaneamente (1 em S1, S2 e R1). A análise deses "spots" por expectrometria de massas em sistema MALDI/TOF-TOF identificou 49 "spots" (61,3%), os quais correspondem a 32 proteínas distintas e 7 proteínas hipotéticas. Entre proteínas identificadas, a peroxirredoxina (expressão aumentada em R) e uma cistéino peptidase semelhante à calpaína (exclusica do grupo S) forma destacadas por indicar que os isolados resistentes são menos susceptíveis ao processo de morte celular programada. Estes dados sugerem que estas proteínas podem estar relacionadas com o fenótipo resistência à miltefosina. PALAVRAS CHAVES: Leishmania chagasi, miltefosina, resistência, proteoma

A non-systematic approach

Funding Information: This work benefited from access to CERM/CIRMMP, the Instruct-ERIC Italy centre. Financial support was provided by European EC Horizon 2020 TIMB3 (Project 810856) Instruct-ERIC (PID 4509). This article is based upon work from COST Action CA15133, supported by COST (European Cooperation in Science and Technology) . Fondazione Ente Cassa di Risparmio di Firenze ( CRF 2016 0985 ) is acknowledged for providing fellowship to MI. This work was funded by national funds through FCT– Fundação para a Ciência e a Tecnologia , I.P., Project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020, and Fundação para a Ciência e a Tecnologia (FCT) Portugal is acknowledged for Grant PD/BD/135187/2017 to IBT. Funding Information: This work benefited from access to CERM/CIRMMP, the Instruct-ERIC Italy centre. Financial support was provided by European EC Horizon 2020 TIMB3 (Project 810856) Instruct-ERIC (PID 4509). This article is based upon work from COST Action CA15133, supported by COST (European Cooperation in Science and Technology). Fondazione Ente Cassa di Risparmio di Firenze (CRF 2016 0985) is acknowledged for providing fellowship to MI. This work was funded by national funds through FCT? Funda??o para a Ci?ncia e a Tecnologia, I.P. Project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020, and Funda??o para a Ci?ncia e a Tecnologia (FCT) Portugal is acknowledged for Grant PD/BD/135187/2017 to IBT. Publisher Copyright: © 2020 The Author(s) Copyright: Copyright 2020 Elsevier B.V., All rights reserved.The complete assignment of 1H, 13C and 15N protein signals, which is a straightforward task for diamagnetic proteins provided they are folded, soluble and with a molecular mass below 30,000 Da, often becomes an intractable problem in the presence of a paramagnetic center. Indeed, the hyperfine interaction quenches signal intensity; this prevents the detection of scalar and dipolar connectivities and the sequential assignment of protein regions close to the metal ion(s). However, many experiments can be optimized and novel experiments can be designed to circumvent the problem and to revive coherences invisible in standard experiments. The small HiPIP protein PioC provides an interesting case to address this issue: the prosthetic group is a [Fe4S4]2+ cluster that is bound to the 54 amino acids protein via four cysteine residues. The four cluster-bound cysteine residues adopt different binding conformations and therefore each cysteine is affected by paramagnetic relaxation to different extent. A network of tailored experiments succeeded to obtain the complete resonance assignment of cluster bound residues.publishersversionpublishe

Experimental research on masonry wall and timber elements connection

Studies developed for the past years and recent earthquakes have brought to light the importance that efficient connections between structural elements can have in the global behaviour of the structure. Although out-of-plane mechanisms of masonry walls are considered to be local, the reality is that their occurrence can lead to total collapse of the structure. The scarcity of data, both at experimental and numerical levels, introduces an urgent need to characterize the response of connections to seismic actions and understand their impact on the overall behaviour of the structure. Therefore, a total of 15 quasi-static monotonic and cyclic pull-out tests were performed on representative wall-to-floor (timber pavement beams) and wall-to-timber framed wall connections, unstrengthened and strengthened, in order to assess their performance and allow their characterization. The analysis contemplates parameters like: failure mode, hysteretic curve, strength degradation and total energy. The study of these parameters promotes better understanding of this type of connection and also the development of design recommendations for the strengthening. As further development, the experimental data will allow calibration of representative numerical models, enabling parametric studies of material properties and formulation of backbone curves

Pastejo e a estabilidade de pastagens naturais.

Pastejo e a Estabilidade de Pastagens Naturais; Comunidades Vegetais Campestres: Conceito; Dinâmica da Vegetação; Distúrbios: Pastejo; Pastejo e Invasão de Ecossistemas Campestres.bitstream/item/76924/1/DT125.pd