Structural design of a vertical antenna boresight 18.3 by 18.3-m planar near-field antenna measurement system

A large very precise near-field planar scanner was proposed for NASA Lewis Research Center. This scanner would permit near-field measurements over a horizontal scan plane measuring 18.3 m by 18.3 m. Large aperture antennas mounted with antenna boresight vertical could be tested up to 60 GHz. When such a large near field scanner is used for pattern testing, the antenna or antenna system under test does not have to be moved. Hence, such antennas and antenna systems can be positioned and supported to simulate configuration in zero g. Thus, very large and heavy machinery that would be needed to accurately move the antennas are avoided. A preliminary investigation was undertaken to address the mechanical design of such a challenging near-field antenna scanner. The configuration, structural design and results of a parametric NASTRAN structural optimization analysis are contained. Further, the resulting design was dynamically analyzed in order to provide resonant frequency information to the scanner mechanical drive system designers. If other large near field scanners of comparable dimensions are to be constructed, the information can be used for design optimization of these also

Development and application of a self-referencing glucose microsensor for the measurement of glucose consumption by pancreatic ?-cells

Glucose gradients generated by an artificial source and ?-cells were measured using an enzyme-based glucose microsensor, 8-?m tip diameter, as a self-referencing electrode. The technique is based on a difference measurement between two locations in a gradient and thus allows us to obtain real-time flux values with minimal impact of sensor drift or noise. Flux values were derived by incorporation of the measured differential current into Fick's first equation. In an artificial glucose gradient, a flux detection limit of 8.2 ± 0.4 pmol·cm-2·s-1 (mean ± SEM, n = 7) with a sensor sensitivity of 7.0 ± 0.4 pA/mM (mean ± SEM, n = 16) was demonstrated. Under biological conditions, the glucose sensor showed no oxygen dependence with 5 mM glucose in the bulk medium. The addition of catalase to the bulk medium was shown to ameliorate surface-dependent flux distortion close to specimens, suggesting an underlying local accumulation of hydrogen peroxide. Glucose flux from ?-cell clusters, measured in the presence of 5 mM glucose, was 61.7 ± 9.5 fmol·nL-1·s-1 (mean ± SEM, n = 9) and could be pharmacologically modulated. Glucose consumption in response to FCCP (1 ?M) transiently increased, subsequently decreasing to below basal by 93 ± 16 and 56 ± 6%, respectively (mean ± SEM, n = 5). Consumption was decreased after the application of 10 ?M rotenone by 74 ± 5% (mean ± SEM, n = 4). These results demonstrate that an enzyme-based amperometric microsensor can be applied in the self-referencing mode. Further, in obtaining glucose flux measurements from small clusters of cells, these are the first recordings of the real-time dynamic of glucose movements in a biological microenvironment. <br/

A Machine Learning-Based Method for Modelling a Proprietary SO2 Removal System in the Oil and Gas Sector

The aim of this study is to develop a model for a proprietary SO2 removal technology by using machine learning techniques and, more specifically, by exploiting the potentialities of artificial neural networks (ANNs). This technology is employed at the Eni oil and gas treatment plant in southern Italy. The amine circulating in this unit, that allows for a reduction in the SO2 concentration in the flue gases and to be compliant with the required specifications, is a proprietary solvent; thus, its composition is not publicly available. This has led to the idea of developing a machine learning (ML) algorithm for the unit description, with the objective of becoming independent from the licensor and more flexible in unit modelling. The model was developed in MatLab® by implementing ANNs and the aim was to predict three targets, namely the flow rate of SO2 that goes to the Claus unit, the emissions of SO2, and the flow rate of steam sent to the regenerator reboiler. These represent, respectively, the two physical outputs of the unit and a proxy variable of the amine quality. Three different models were developed, one for each target, that employed the Levenberg–Marquardt optimization algorithm. In addition, the ANN topology was optimized case by case. From the analysis of the results, it emerged that with a purely data-driven technique, the targets can be predicted with good accuracy. Therefore, this model can be employed to better manage the SO2 removal system, since it allows for the definition of an optimal control strategy and the maximization of the plant’s productivity by not exceeding the process constraints

Enamel fluorosis in rat’s incisor: S.E.M. and T.E.M. investigation

Findings on the alterations taking place in the enamel have demonstrated that they are generally caused by a daily use of highly fluoritic drinking waters.According to that, the Authors have carried out an ultrastructural study on lower incisors of albino rats after administering for 60 days water with a fluorine concentration five times the normal one.The samples, studied under the S.E.M., showed a general slowing of both the deposition and the maturation phase as well as the presence of some hypomineralized areas even after eruption.All this suggested the possibility that the damages observed were not due to the direct effect of fluorine on the enamel, but to the interaction between fluorine and ameloblasts.The Authors have then carried out an ultrastructural study on the enamel organ using the S.E.M.The results showed the presence of a well-evident endoplasmic reticulum, the lack in dense granules during the secretion phase, the lack in ruffle ended webs during the modulation phase, and the mitochondrial damage in the ameloblast.All this could justify the slowing of the enamel mineralization caused by fluorine effect on the ameloblasts.Il a été démontré que des altérations de l’émail sont généralement dues à l’absorption quotidienne d’eaux de boissons riches en fluor.Les auteurs ont entrepris une étude ultrastructurale des incisives inférieures du rat ayant reçu pendant 60 jours de l’eau dont la concentration en fluor est 5 fois supérieure à la normale.Les échantillons étudiés en microscopie électronique à balayage ont montré un ralentissement global de la déposition et de la phase de maturation ainsi que la présence de territoires hypominéralisés même après l’éruption.Toutes ces constatations suggèrent la possibilité que les dommages observés ne sont pas dus à l’effet direct du fluor sur l’émail, mais aux interactions entre le fluor et les améloblastes.Les auteurs ont mené une étude ultrastructurale de l’émail en utilisant le microscope électronique à balayage.Les résultats ont montré la présence d’un réticulum endoplasmique bien apparent, l’absence de grains denses au cours de la phase secrétoire, l’absence d’extrémités membranaires rugueuses au cours de la phase de modulation, et des altérations des mitochondries dans les améloblastes.Toutes ces constatations pourraient expliquer le ralentissement de la minéralisation de l’émail causé par les effets du fluor sur les améloblastes

Endovascular stenting of the ascending aorta for type A aortic dissections in patients at high risk for open surgery

Background: Open repair is the gold standard for type A aortic dissection (TAAD). Endovascular option has been proposed in very limited and selected TAAD patients. We report our experience with endovascular TAAD repair. Methods: Inclusion criteria were: (1) entry tear in the ascending aorta; (2) proximal landing zone of at least 2 cm; (3) distance between entry tear and brachio-cephalic trunk of at least 0.5 cm; (4) no signs of cardiac tamponade or severe aortic regurgitation and (5) no signs of aortic branches ischaemia. Patients with cardiac revascularisation from ascending aorta were excluded. Results: From April 2009 to June 2012, 37 patients with TAAD were admitted to our hospital. As many as 28 underwent surgical repair and 9 were considered at high surgical risk in a multidisciplinary meeting. Four met our inclusion criteria for an endovascular approach. Two of them had previous ascending aortic repair for TAAD and one had aortic valve replacement. Technical success was achieved in 100% of the patients. No mortality was registered during a median follow-up of 15 months (range 4-39 months), no migration of the graft and complete false lumen thrombosis of the ascending aorta in three patients. Conclusion: Endovascular treatment of TAAD is challenging but feasible in a selected subset of patients. Further research remains mandatory. © 2013 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved

The tyrosine kinase receptor c-met, its cognate ligand HGF and the tyrosine kinase receptor trasducers STAT3, PI3K and RHO in thyroid nodules associated with Hashimoto's thyroiditis: an immunohisto-chemical characterization

Hepatocyte growth factor (HGF) exerts proliferative activities in thyrocytes upon binding to its tyrosine kinase receptor c-met and is also expressed in benign thyroid nodules as well as in Hashimoto's thyroiditis (HT)

A non-invasive method for measuring preimplantation embryo physiology

Author Posting. © Cambridge University Press, 2000. This article is posted here by permission of Cambridge University Press for personal use, not for redistribution. The definitive version was published in Zygote 8 (2000): 15-24, doi:10.1017/S0967199400000782.The physiology of the early embryo may be indicative of embryo vitality and therefore methods for non-invasively monitoring physiological parameters from embryos could improve preimplantation diagnoses. The self-referencing electrophysiological technique is capable of non-invasive measurement of the physiology of individual cells by monitoring the movement of ions and molecules between the cell and the surrounding media. Here we use this technique to monitor gradients of calcium, potassium, oxygen and hydrogen peroxide around individual mouse preimplantation embryos. The calcium-sensitive electrode in self-referencing mode identified a region of elevated calcium concentration ([similar]0.25 pmol) surrounding each embryo. The calcium gradient surrounding embryos was relatively steep, such that the region of elevated calcium extended into the medium only 4 [mu]m from the embryo. By contrast, using an oxygen-sensitive electrode an extensive gradient of reduced dissolved oxygen concentration was measured surrounding the embryo and extended tens of micrometres into the medium. A gradient of neither potassium nor hydrogen peroxide was observed around unperturbed embryos. We also demonstrate that monitoring the physiology of embryos using the self-referencing technique does not compromise their subsequent development. Blastocysts studied with the self-referencing technique implanted and developed to term at the same frequency as did unexamined, control embryos. Therefore, the self-referencing electrode provides a valuable non-invasive technique for studying the physiology and pathophysiology of individual embryos without hindering their subsequent development.A portion of this work was funded by an NIH R21 #RR 12718–02 to D.L.K. and P.J.S.S., KO81099 to D.L.K. and NIH P41 RR01395 to P.J.S.S

A decision support system for Rey-Osterrieth complex figure evaluation

Objective: The Rey Osterrieth complex figure (ROCF) is one of the most used neuropsychological tests for the assessment of mild cognitive impairment (MCI) and dementia. In the copy test, the patient has to draw a replica of a 18-pattern image and the outcome is a score based on the accuracy of the overall drawing. The standard scoring system however have limitations related to its subjective nature and its inability to evaluate other cognitive domains than constructional abilities. Previous works addressed those problems by proposing tablet-based automated evaluation systems. Even promising, such methods are still far away from clinical validation and translation. In this work, we developed a decision support system (DSS) for the evaluation of the ROCF copy test in the common practice using retrospective information from previously performed drawings. The goal of our system was to support the professionals providing a qualitative judgement for each of the 18 patterns, estimating the most probable diagnosis for the patient, and identifying the main signs associated to the obtained diagnosis. Methods: A total of 250 human evaluated ROCF copies were scanned from 57 healthy subjects, 131 individuals with MCI, and 62 individuals with dementia. The images were pre-processed and analysed using both computer vision and deep learning techniques to assign a qualitative label to the 18 patterns. Then, the 18 labels were used as features in 3 binary (healthy VS MCI, healthy VS dementia, MCI VS dementia) and a 3-class classifications with model explanation (SHAP).Results: Very good to excellent performance were obtained in all the diagnosis classification tasks. Indeed, an accuracy of about 85%, 91%, and 83% was obtained in discriminating healthy subjects from MCI, healthy subjects from dementia and MCI from dementia respectively. An accuracy of 73% was achieved in the 3-class classification. The model explanation showed which patterns are responsible for each prediction and how the importance of some patterns changes according to the severity of the cognitive decline. Significance: The proposed DSS enriches the standard evaluation and interpretation of the ROCF copy test. Being trained with retrospective knowledge, the performance of the DSS can be further enhanced by extending the dataset with existing ROCF copies

Immunohistochemical localization and functional characterization of somatostatin receptor subtypes in a corticotropin releasing hormone-secreting adrenal phaeochromocytoma: review of the literature and report of a case

Somastostatin receptors are frequently expressed in phaeochromocytoma but data on somatostatin receptor subtyping are scanty and the functional response to the somatostatin analogue octretide is still debated.We report an unusual case of pheochromocytoma, causing ectopic Cushing’s syndrome due to CRH production by the tumour cells, in a 50-yr-old woman. Abdominal computed tomography revealed an inhomogeneous, 9-cm mass in the right adrenal gland, and [111In-DTPA0] octreotide scintigraphy showed an abnormal uptake of the radiotracer in the right perirenal region, corresponding to the adrenal mass. The patient underwent laparoscopic surgery and formalin-fixed and paraffin-embedded samples were studied. The tumour was extensively characterized by immunohistochemistry and somatostatin receptor (SSTRs) subtypes expression was analyzed. Histological and immunohistochemical examination of the surgical specimens displayed a typical pheochromocytoma, which was found to be immunoreative to S-100, chromogranin A and neurofilaments. Immunostaining for SSTR subtypes showed a positive reaction for SSTR1, SSTR2A, SSTR2B, antisera on tumour cells. The intense and diffuse immunostaining for corticotropin releasing hormone (CRH) antiserum indicated that Cushing’s disease was dependent on CRH overproduction by the pheochromocytoma, in which no immunostaining for adrenocorticotropic hormone was found. Our report confirms the heterogeneity of the pattern of SSTR expression in pheochromocytomas, and provide further evidence for functional SSTR subtype SSTR2a in a subgroup of pheochromocytomas, suggesting that these tumours may represent potential target for octreotide treatment