A transgenic plant having enhanced drought tolerance

The present invention relates to the field of transgenic plants with novel phenotypes, especially plants with enhanced drought and pathogen resistance. Provided are transgenic crop plants comprising integrated in their genome a chimeric gene, characterized by said chimeric gene comprising a transcription regulatory sequence active in plant cells operably linked to a nucleic acid sequence encoding a protein having the sequence of SEQ ID NO: 3 or a protein at least 70 % identical to SEQ ID NO: 3, or an ortholog protein or a functional fragment thereof. In addition to enhanced drought tolerance the transgenic plants may show enhanced disease resistance and enhanced root structure

Identifikasi Galur dan Gen-gen Terkait Toleran Kekeringan pada Padi Transgenik cv. T309 yang Mengandung Vektor Penanda Aktivasi

Activation tagging is an efficient tool forfunctional analysis of the rice genes. We have developed anumber of transgenic rice lines (Oryza sativa L. ssp.japonica cv. Taipei 309) containing activation tagging vector.However, the phenotypes and genotypes of these lines, inrelation to the drought stress, have not been analyzed. Theobjectives of this research were to identify transgenic ricelines that showed tolerance to the drought stress and toidentify the genes that may be associated with the droughtstress. The drought stress tolerance in transgenic rice lineswas identified by testing their tolerance to the drought stressand also by detecting the presence of bar and nptII genes.The result showed that 56 out of 59 rice lines were resistantto Basta herbicide and three of them showed tolerance todrought stress, namely PA.T-1.2, PA.T-4.1, and PA.T-5.1 lines.PCR analysis showed that PA.T-1.2 and PA.T-4.1 containedboth hptII and bar genes, while the PA.T-5.1 line containedbar gene only. Thermal Asymetric Interlaced-PCR (TAILPCR)analysis showed that two genes may be asssociatedwith the drought stress tolerance. Those genes areOSJNBa0004120.14 that produces uridylate putative kinaseand OsPPCK2L that produces phosphoenolpyruvatecarboxylase kinase

Pengaruh Material Abrasif Pada Blasting Terhadap Kekuatan Lekat Cat Dan Ketahanan Korosi Di Lingkungan Air Laut

Coating merupakan proses pelapisan pada permukaan profil material dengan suatu lapisan film organik maupun anorganik. Coating lebih sering digunakan pada industry karena lebih ekonomis dan lebih mudah digunakan. Namun teknik ini pun tidak lepas dari berbagai masalah. Masalah yang timbul dari teknik ini sebagian besar disebabkan karena buruknya proses surface preparation yang dapat mengurangi daya lekat cat. Buruknya proses surface preparation dapat disebabkan berbagai hal antara lain ukuran mesh dari material abrasif yang tidak pas, pemilihan material abrasif, hingga kesalahan teknik dalam melakukan proses ini. Penelitian ini dilakukan untuk menganalisa pengaruh dari material abrasif terhadap kekuatan lekat cat di material A36 yang diberikan variasi jenis material abrasif alumunium oxide, steel grit, dan volcanic sand, kemudian material di coating dengan cat jenis epoxy dan zinc rich. Hasil dari pengujian menunjukan bahwa peningkatan kekerasan material abrasif meningkatkan kekasaran permukaan pada substrat. Nilai rata-rata kekasaran permukaan yang tertinggi adalah material yang di blasting dengan steel grit yaitu sebesar 84.71 μm. Meningkatnya kekasaran permukaan akan menambah daya lekat cat dengan substrat, yang juga akan meningkatkan ketahanan korosi pada material. Nilai daya lekat tertinggi pada material yang di coating dengan cat jenis epoxy dan zinc rich berturut-turut sebesar 20.04 MPa dan 21.46 MPa, dimana material tersebut di blasting dengan menggunakan steel grit. Hasil dari pengujian salt spray NaCl 3.5% selama 72 jam, dan 168 jam pada material menunjukan material yang di blasting dengan steel grit tetap memiliki nilai daya lekat yang tertinggi. Nilai daya lekat tertinggi setelah uji salt spray selama 72 jam pada material yang di coating dengan cat jenis epoxy dan zinc rich berturut-turut sebesar 14.15 MPa dan 15.04 MPa, sedangkan Nilai daya lekat tertinggi setelah uji salt spray selama 168 jam pada material yang di coating dengan cat jenis epoxy dan zinc rich berturut-turut sebesar 11.91 MPa dan 12.80 MPa. ========================================================================================================== Coating is a covering that is applied to the surface of an object, usually referred to as the substrate. Commonly, coating is used in the industry because it is more economical and easier to used. However, this technique is also has a lot of problems. Problems arise from these techniques due to poor surface preparation process that can reduce the adhesion of paint. A poor surface preparation process can be caused by various things including a mesh size of abrasive material, abrasive material selection, and human errors. This research was conducted to analyze the effect of abrasive material for blasting against the adhesive strength of the paint in ASTM A36 which was blasted with varying abrasive material i.e aluminum oxide, steel grit, and volcanic sand, and was coated with different paint types i.e epoxy paint and zinc rich paint. The results showed that the increasing of abrasive material hardness can increase the surface roughness. And increasing surface roughness will increase adhesion of paint to the substrate, which will improve the corrosion resistance of the material. The highest surface roughness value , 84.71 μm, and the highest adhesion value in specimens which coated with epoxy paint and zinc rich paint, consecutively 20.04 MPa and 21.46 MPa possessed by the specimens which use steel grit as an abrasive material for blasting. The results of salt spray testing with NaCl 5% for 72 hours, and 168 hours on the specimens shows that specimens which blasted with steel grit still has the highest adhesion values. The highest adhesion value after the salt spray test for 72 hours in specimens which coated with epoxy paint and zinc rich paint, consecutively 14.15 MPa and 15.05 Mpa. While the highest adhesion value after the test salt spray for 168 hours on the material in the coating with epoxy paint and zinc rich painted consecutively 11.91 MPa and 12.80 MPa

Pengembangan Populasi Mutan Penanda Aktivasi: I. Transformasi Padi Japonica Tropis Lokal Sulawesi CV. Asemandi Dengan Bantuan Agrobacterium Tumefaciens

The rice transformation technologyis not only provides valuable methods for the introductionof useful genes into rice plant to improve importantagronomic traits, but also helps in studying gene functionand regulation based on rice genome sequence information.Knockout of genes by insertional mutagenesis is a straightforwardmethod to identify gene functions. One of themethods to develop rice mutants is through genetic transformationmediated by Agrobacterium using activationtagging by Ac-Ds system. A study was done with an objectiveto obtain mutant rice of local tropical japonica cv. Asemandithrough genetic trans-formation mediated by Agrobacteriumtumefaciens. The transformation was conducted usingAgrobacterium vector with the strain of Agl-1 containingactivation tag construct. The result of experiment showedthat it has been obtained 17 independent line (304 plants)transgenic Asemandi containing activation tag construct.These starter lines will be used as materials to developseveral generations of stabil rice mutant through selfing

Introduksi Konstruk Gen CsNitr1-L dengan Promotor Ubiquitin melalui Agrobacterium Tumefaciens dan Deteksi Molekulernya pada Padi Kultivar Nipponbare

Nitrogen based fertilizers such as urea and NPK are primary needs for rice farmers. To get significant improvement of crop yield, the more quantity of fertilizers are applied. It make negative impact for surrounding environment. Based on that, the efforts should be done to suppress the demand of fertilizers such as by developing Nitrogen Use Efficiency crops. CsNitr1-L is one of gene that related to Nitrogen Use Efficiency trait in plant. The objectives of this research are to develop the construction of CsNitr1-L gene candidate in pCAMBIA1300-Ubi1 promoter and to obtain the transformants of rice cultivar Nipponbare which contain the construction of CsNitr1-L gene candidate. The construction of pCAMBIA1300::Ubi1::CsNitr1-L has successfully assembled and was transformed to immature embryo of rice cultivar Nipponbare using Agrobacterium tumefaciens strain LBA4404. It was obtained 146 lines of T0 Nipponbare. PCR analysis of T0 Nipponbare lines showed that 66 of them was identified as positive T0 lines contained hptII and CsNitr1-L genes. Transformation efficiency obtained was 11,9%. The result of analysis copy number using Southern Hybridization in positive PCR of T0 lines randomly indicated that 4 lines have a single copy of transgene. Based on these results, it can be concluded that CsNitr1-L gene construct was successfully introduced into the genome of the rice plant cultivar Nipponbare and the positive PCR of T0 lines containing the gene of hptII and CsNitr1-L, also a single copy of the transgene was obtained

Respon Padi Transgenik CV. Nipponbare Generasi T1 Yang Mengandung Gen Oryza Sativa Dehydration-response Element Binding 1a (Osdreb1a) Terhadap Cekaman Salinitas [Response of T1 Generation Transgenic Rice CV. Nipponbare Containing an Oryza Sativa Dehydration-response Element Binding 1a (Osdreb1a) Gene to Salinity Stress]

Salinity is one of the abiotic constraints in the cultivation of rice crop. One of the reasons agricultural land becomes saline is due to the intrusion of seawater into the mainland as a result of global climate change. Dehydration-responsive element binding (DREB) gene is a plant -specific transcription factor gene that have important role in regulating plant responses to abiotic stresses, including high salinity. Transgenic rice plants cv. Nipponbare carrying OsDREB1A gene have been generated. However, study of the response of putative transgenic plants to salinity has not been done. The research objective is to study the response of T1 generation Nipponbare-OsDREB1A transgenic rice plants to salinity stress. The result showed that the response of putative transgenic rice Nipponbare-OsDREB1A to salinity stress 25 mM and 150 mM NaCl indicated a level of tolerance varies from highly sensitive to highly tolerance. These variations were possibly occurred because of the segregation state of the T1 generation transgenic rice. Based on damage symptom scoring and PCR analysis provided information that transgenic rice plant cv. Nipponbare-OsDREB1A which showed positive PCR had a very high tolerance to salinity stress 150 mM compared with non-transgenic rice cv. Nipponbare