Optimization of ultrasound-assisted extraction of bioactive compounds from Pelvetia canaliculata to sunflower oil

In this study, Pelvetia canaliculata L. macroalga, collected from the Atlantic Portuguese coast, was used as a source of bioactive compounds, mostly antioxidants, to incorporate them in sunflower oil with the aim of increasing its biological value and oxidative stability. The lyophilized alga was added to the oil, and ultrasound-assisted extraction (UAE) was performed. Algae concentration and UAE time varied following a central composite rotatable design (CCRD) to optimize extraction conditions. The following parameters were analyzed in the oils: oxidation products, acidity, color, chlorophyll pigments, carotenoids, flavonoids, total phenolic content, antioxidant activity by DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) assays, and sensory analysis. Extraction conditions did not affect the acidity and the amount of oxidation products in the oil. Chlorophylls and carotenoid contents increased with algae concentration, while flavonoid extraction did not depend on algae content or UAE time. Total phenolics in the oil were highly related only to FRAP antioxidant activity. Storage experiments of supplemented oil (12.5% algae; 20 min UAE) were carried out under accelerated oxidation conditions at 60 C/12 days. Antioxidant activity (FRAP) of supplemented oil was 6-fold higher than the value of non-supplemented oil. Final samples retained 40% of their initial antioxidant activity. The presence of algae extracts contributed to the increased oxidative stability of sunflower oilinfo:eu-repo/semantics/publishedVersio

From Brussels to Belgrade: Challenges in Conducting Research and Constructing Explanations of the Collapse of Yugoslavia

While acknowledging that it is important to examine events within their appropriate context, this article is interested in the capacity of qualitative research methods to assist us so that we can get a more accurate picture of European Community involvement in the Yugoslav federation and the decisions that terminated its existence. More precisely, the article is concerned with the extent to which archival collections and interviews with state as well as nonstate actors can shape our ideas and consequent explanations of the Yugoslav state crisis. In addition, the last section elaborates on a number of challenges one may encounter while being on such a demanding research journey. As suggested by the concluding remarks, new interpretations, apart from managing to satisfy the researcher’s own ambition to complement the existing scholarship, should also serve to encourage fresh questions and answers

The Principle of Non-Interference and Cyber Operations

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The Law of International Organizations. Documents and Cases

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