Optimization of a Bioremediation Strategy for an Urban Stream of Matanza- Riachuelo Basin

In the present work, a remediation bioprocess based on the use of a local isolate of the microalgae Chlorella vulgaris immobilized in alginate beads is proposed. This process was shown to be effective for the reduction of several chemical and microbial contaminants present in Cildáñez stream, a water course that is part of the Matanza-Riachuelo Basin (Buenos Aires, Argentina). The bioprocess, involving the culture of the microalga in autotrophic conditions in a stirred-tank bioreactor supplied with a marine propeller for 6 days, allowed a significant reduction of Escherichia coli and total coliform numbers (over 95%), as well as of ammoniacal nitrogen (96%), nitrates (86%), nitrites (98%), and total phosphorus (53%) contents. Pb content was also significantly diminished after the bioprocess (95%). Standardized cytotoxicity tests using Allium cepa seeds and Cildáñez water pre- and post-remediation were also performed. Germination rate and mitotic index of onion seeds imbibed in Cildáñez water subjected to the bioprocess was similar to that observed in seeds imbibed in distilled water and significantly superior to that registered when untreated Cildáñez water was used for imbibition. Our results demonstrate the potential of this simple and cost-effective technology to remove urban-water contaminants, offering as an additional advantage the possibility of an easy biomass recovery, which may become a source of alternative energy.Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Trentini, Andrea Giannina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Zawoznik, Myriam Sara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Bigi, Roxana Ines. Agencia de Protección Ambiental; Argentina. Centro de Información y Formación Ambiental; ArgentinaFil: Nadra, Carlos. Autoridad de Cuenca Matanza Riachuelo.; ArgentinaFil: Marconi, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaInternational Conference on Bioremediation and BiodegradationEstambulTurquíaWorld Academy of Science, Engineering and Technolog

Development and testing of a 3D-printable polylactic acid device to optimize a water bioremediation process

In the present work, a remediation bioprocess based on the use of a native isolate of Chlorella vulgaris immobilized in an alginate matrix inside a polylactic acid (PLA) device is proposed. This microalga immobilized in alginate beads was previously shown to be useful for the reduction of several chemical and microbial contaminants present in the highly polluted water from the Matanza–Riachuelo watershed. However, these beads had a relatively short shelf life in the natural environment. To overcome this limitation, a 3D-printed PLA device was designed. PLA is a biocompatible and biodegradable material suitable for biotechnological applications. We used Erlenmeyers and stirred-tank bioreactors fed batch with Murashige Skoog (MS) culture medium or water from the Cildáñez stream (one of the water bodies of the aforementioned watershed) to estimate the growth kinetics parameters and the bioremediation capacity of immobilized-microalgal cells as an unconfined system (UcS) or a confined system (CfS) inside PLA devices on Cildáñez water. Although alga’s growth parameters were maximum in the UcS fed with MS medium as substrate, successful bioremediation of the target water was possible using the CfS: all inorganic nitrogen forms and total phosphorus were reduced at least by 90% after 5 days of bioprocess in an agitated bioreactor, whereas aerobic mesophilic bacteria decreased by about 85%. The number of coliforms also decreased. Standardized cytotoxicity tests using Allium cepa seeds carried out to prove the effectiveness of the bioremediation process, confirmed the high degree of decontamination achieved by the use of immobilized microalga confined in a 3D-printable PLA-device.Fil: Marconi, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Trentini, Andrea Giannina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Zawoznik, Myriam Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Nadra Chaud, Carlos Alberto. Universidad Nacional de Tres de Febrero; Argentina. Autoridad de Cuenca Matanza Riachuelo.; ArgentinaFil: Mercadé, Juan José. Agencia de Protección Ambiental; ArgentinaFil: Sanchez Novoa, Juan Gabriel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Orozco, Daniel. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentin

Blackwater Fever in Children, Burundi

Blackwater fever is characterized by acute intravascular hemolysis with hemoglobinuria in patients with Plasmodium falciparum malaria. Its pathogenesis and management are still debated. Nine cases of this syndrome occurred in 2003 at Kiremba Hospital in Burundi in children receiving multiple quinine treatments

Apuntes antropológicos sobre pueblos indígenas y violencias en la Argentina contemporánea

Constantemente, comunidades y organizaciones indígenas en nuestro país denuncian acciones violentas implementadas por las fuerzas de seguridad, empresarios y terratenientes, entre otros actores sociales. La violencia iniciada con la conquista y colonización española en el siglo XVI se intensificó durante la consolidación del Estado nación en la segunda mitad de siglo XIX, en colaboración con las elites y las iglesias. La violencia contra los pueblos indígenas no ha cesado, sino que continúa hoy a través del despojo territorial y el desplazamiento forzado, que se actualizan a medida que el modelo neoextractivista se profundiza y amplía las fronteras de las tierras a explotar...Fil: Lenton, Diana Isabel. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias Antropológicas. Sección de Etnología y Etnografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodriguez, Mariela Eva. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias Antropológicas. Sección de Etnología y Etnografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Szulc, Andrea Paola. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias Antropológicas. Sección de Etnología y Etnografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Matarrese, Marina Laura. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias Antropológicas. Sección de Etnología y Etnografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trentini, Florencia. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias Antropológicas. Sección de Etnología y Etnografía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tolosa, Sandra. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias Antropológicas. Sección de Etnología y Etnografía; Argentina. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias de la Educación; ArgentinaFil: Aguzin, Cecilia Solange. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias Antropológicas. Sección de Etnología y Etnografía; ArgentinaFil: Elichiry, Valeria. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias Antropológicas. Sección de Etnología y Etnografía; ArgentinaFil: Goñi, Julián. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Instituto de Ciencias Antropológicas. Sección de Etnología y Etnografía; Argentin

Attività di testing rapido HIV al Pride di Torino per determinare politiche strategiche e organizzative tra gli MSM

L’offerta del test HIV presso un evento sociale frequentato prevalentemente da giovani uomini che fanno sesso con uomini (MSM) fornisce una possibilità unica di intervenire nel processo di diagnosi precoce dell’HIV. Il progetto Torino Fast Track City permette di osservare elementi organizzativi utili per l’organizzazione dei test rapidi sul territorio, al tempo stesso l’adesione a COBATEST Network fornisce la possibilità di osservare un campione determinando le variabili utili per la costruzione di strategie di intervento e campagne di prevenzione. La somministrazione dei test rapidi HIV da parte delle associazioni è uno strumento chiave per incrementare il numero di soggetti che si sottopongono al test, accedono alle cure precocemente e vengono informati sulle terapie e sui possibili comportamenti a rischio

Is "option B+" also being adopted in pregnant women in high-income countries? Temporal trends from a national study in Italy

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The Sex-Specific Detrimental Effect of Diabetes and Gender-Related Factors on Pre-admission Medication Adherence Among Patients Hospitalized for Ischemic Heart Disease: Insights From EVA Study

Background: Sex and gender-related factors have been under-investigated as relevant determinants of health outcomes across non-communicable chronic diseases. Poor medication adherence results in adverse clinical outcomes and sex differences have been reported among patients at high cardiovascular risk, such as diabetics. The effect of diabetes and gender-related factors on medication adherence among women and men at high risk for ischemic heart disease (IHD) has not yet been fully investigated.Aim: To explore the role of sex, gender-related factors, and diabetes in pre-admission medication adherence among patients hospitalized for IHD.Materials and Methods: Data were obtained from the Endocrine Vascular disease Approach (EVA) (ClinicalTrials.gov Identifier: NCT02737982), a prospective cohort of patients admitted for IHD. We selected patients with baseline information regarding the presence of diabetes, cardiovascular risk factors, and gender-related variables (i.e., gender identity, gender role, gender relations, institutionalized gender). Our primary outcome was the proportion of pre-admission medication adherence defined through a self-reported questionnaire. We performed a sex-stratified analysis of clinical and gender-related factors associated with pre-admission medication adherence.Results: Two-hundred eighty patients admitted for IHD (35% women, mean age 70), were included. Around one-fourth of the patients were low-adherent to therapy before hospitalization, regardless of sex. Low-adherent patients were more likely diabetic (40%) and employed (40%). Sex-stratified analysis showed that low-adherent men were more likely to be employed (58 vs. 33%) and not primary earners (73 vs. 54%), with more masculine traits of personality, as compared with medium-high adherent men. Interestingly, women reporting medication low-adherence were similar for clinical and gender-related factors to those with medium-high adherence, except for diabetes (42 vs. 20%, p = 0.004). In a multivariate adjusted model only employed status was associated with poor medication adherence (OR 0.55, 95%CI 0.31–0.97). However, in the sex-stratified analysis, diabetes was independently associated with medication adherence only in women (OR 0.36; 95%CI 0.13–0.96), whereas a higher masculine BSRI was the only factor associated with medication adherence in men (OR 0.59, 95%CI 0.35–0.99).Conclusion: Pre-admission medication adherence is common in patients hospitalized for IHD, regardless of sex. However, patient-related factors such as diabetes, employment, and personality traits are associated with adherence in a sex-specific manner