Expression of the Ladybird-like homeobox 2 transcription factor in the developing mouse testis and epididymis

<p>Abstract</p> <p>Background</p> <p>Homeoproteins are a class of transcription factors that are well-known regulators of organogenesis and cell differentiation in numerous tissues, including the male reproductive system. Indeed, a handful of homeoproteins have so far been identified in the testis and epididymis where a few were shown to play important developmental roles. Through a degenerate PCR approach aimed at identifying novel homeoproteins expressed in the male reproductive system, we have detected several homeoproteins most of which had never been described before in this tissue. One of these homeoproteins is Ladybird-like homeobox 2 (Lbx2), a homeobox factor mostly known to be expressed in the nervous system.</p> <p>Results</p> <p>To better define the expression profile of Lbx2 in the male reproductive system, we have performed <it>in situ </it>hybridization throughout testicular and epididymal development and into adulthood. Lbx2 expression was also confirmed by real time RT-PCR in those tissues and in several testicular and epididymal cell lines. In the epididymis, a highly segmented tissue, Lbx2 shows a regionalized expression profile, being more expressed in proximal segments of the caput epididymis than any other segment. In the testis, we found that Lbx2 is constitutively expressed at high levels in Sertoli cells. In interstitial cells, Lbx2 is weakly expressed during fetal and early postnatal life, highly expressed around P32-P36, and absent in adult animals. Finally, Lbx2 can also be detected in a population of germ cells in adults.</p> <p>Conclusion</p> <p>Altogether, our data suggest that the homeoprotein Lbx2 might be involved in the regulation of male reproductive system development and cell differentiation as well as in male epididymal segmentation.</p

Speech production in aging

The manner and extent to which normal aging affects the ability to speak are not fully understood. While age-related changes in voice fundamental frequency and intensity have been documented, changes affecting the planning and articulation of speech are less well understood. In the present study, 76 healthy, cognitively normal participants aged between 18 and 93 years old were asked to produce auditorily and visually triggered sequences of finely controlled movements (speech, oro-facial, and manual movement). These sequences of movements were either (1) simple, in which at least two of the three movements were the same, or (2) complex, in which three different movements were produced. For each of the resulting experimental condition, accuracy was calculated. The results show that, for speech and oro-facial movements, accuracy declined as a function of age and complexity. For these movements, the negative effect of complexity on performance accuracy increased with age. No aging or complexity effects were found for the manual movements on accuracy, but a significant slowing of movement was found, particularly for the complex sequences. These results demonstrate that there is a significant deterioration of fine motor control in normal aging across different response modalities

Life in mine tailings: microbial population structure across the bulk soil, rhizosphere, and roots of boreal species colonizing mine tailings in northwestern Québec

Abstract Purpose Mining activities have negative effects on soil characteristics and can result in low pH, high heavy metal content, and limited levels of essential nutrients. A tailings storage area located in northwestern Québec showed natural colonization by plants from the adjacent natural environment. The objective of the study was to determine the main edaphic parameters that structured microbial populations associated with the indigenous woody plants that had naturally colonized the site. Methods Microbial populations were studied in the bulk soil, the rhizosphere, and inside plant roots using Illumina sequencing, ordination analysis (i.e., redundancy analysis (RDA) and principal coordinates analysis (PCoA)), ternary plotting, and statistical analysis (MANOVA). Results The main variables that drove the microbial community patterns were plant species and the tailings pH. Indeed, the main bacterial classes were Gammaproteobacteria and Deltaproteobacteria in both the rhizosphere and root endosphere. Analysis revealed that some dominant operational taxonomic units (e.g., Pseudomonas sp., Acinetobacter sp., and Delftia sp.) were present in increased proportions in roots for each plant species under study. This study also revealed that many of the most abundant fungal genera (e.g., Claussenomyces, Eupenicillium, and Trichoderma) were more abundant in the rhizosphere than in the root endosphere. Conclusions This comprehensive study of the microbial community dynamics in the bulk soil, rhizosphere, and root endosphere of boreal trees and shrubs could be beneficial in facilitating the rehabilitation of disturbed ecosystems

How toddlers' irritability and fearfulness relate to parenting : a longitudinal study conducted among Quebec families

Although child difficult temperament is a well‐known risk factor for parenting quality, few studies have focused on the association between specific temperament dimensions and parental behaviours. This study focused on negative emotionality, one of the best‐accepted dimensions of temperament, and its subdimensions of irritability and fearfulness. The aim of the present study was to evaluate the predictive value of irritability and fearfulness at 17 months upon parenting practices (involvement, coercion, and overprotection) at 29 months, beyond the influence of other well‐known risk factors (e.g., socio‐economic status and maternal depression). The study used data from the Quebec Longitudinal Study of Child Development, a longitudinal study conducted upon 1,829 families from Quebec (Canada), using self‐report questionnaires and medical files. Structural equation modelling identified irritability as a predictor of coercion, an externally controlling practice, whereas fearfulness predicted overprotection, an internally controlling practice. No significant associations were found after modelling between dimensions of negative emotionality and involvement. These results underline how certain aspects of child temperament may differentially “pull for control” and lead parents to act in a certain way, which may thwart young children's development and need for autonomy. Highlights This article investigates the associations between negative emotionality (i.e., irritability and fearfulness) and parenting (i.e., involvement, coercion, and overprotection). Structural equation modelling was used on data collected during a longitudinal study with a representative sample of 2,223 families. Each dimension of negative emotionality was associated to a different form of controlling parenting 1 year later (i.e., irritability with coercion and fearfulness with overprotection)

The study of noninvasive brain stimulation using molecular brain imaging: A systematic review

Electromagnetic noninvasive brain stimulation (NIBS) techniques, such as transcranial magnetic stimulation and transcranial electrical stimulation, are widely used in research and represent emerging clinical treatment options for many brain disorders. The brain-wide neurobiological effects of electromagnetic NIBS, however, are not yet fully characterized. The combination of NIBS with molecular brain imaging is a powerful tool for the investigation of these effects. Here, we conducted a systematic review of all published studies investigating the effects of all forms of electromagnetic NIBS using molecular imaging (positron emission tomography, single photon emission computed tomography). A meta-analysis was also conducted when sufficient studies employed similar methodologies. A total of 239 articles were identified, of which 71 were included in the review. Information was extracted about the study design, NIBS parameters, imaging parameters, and observed local and remote effects caused by the stimulation. Regional cerebral blood flow and glucose metabolism were the most common outcome measures, followed by dopamine neurotransmission. While the vast majority of studies obtained remote effects of stimulation in interconnected regions, approximately half of the studies showed local effects at the stimulation site. Our meta-analysis on motor cortex stimulation also showed consistent remote effects. The literature review demonstrates that although the local effects of NIBS as captured by molecular imaging are sometimes modest, there are robust remote changes in brain activity and neurotransmitter function. Finally, we discuss the potential pitfalls and methodological issues and identify gaps in the current knowledge that could be addressed using these techniques

Linking molecular size, composition and carbon turnover of extractable soil microbial compounds

Microbial contribution to the maintenance and turnover of soil organic matter is significant. Yet, we do not have a thorough understanding of how biochemical composition of soil microbial biomass is related to carbon turnover and persistence of different microbial components. Using a suite of state-of-the-art analytical techniques, we investigated the molecular characteristics of extractable microbial biomass and linked it to its carbon turnover time. A 13CO2 plant pulse labelling experiment was used to trace plant carbon into rhizosphere soil microbial biomass, which was obtained by chloroform fumigation extraction (CFE). 13C content in molecular size classes of extracted microbial compounds was analysed using size exclusion chromatography (SEC) coupled online to high performance liquid chromatography–isotope ratio mass spectrometry (SEC-HPLC-IRMS). Molecular characterization of microbial compounds was performed using complementary approaches, namely SEC-HPLC coupled to Fourier transform infrared spectroscopy (SEC-HPLC-FTIR) and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS). SEC-HPLC-FTIR suggests that mid to high molecular weight (MW) microbial compounds were richer in aliphatic CH bonds, carbohydrate-like compounds and possibly Pdouble bond; length as m-dashO derivatives from phospholipids. On the contrary, the lower size range was characterized by more oxidised compounds with hydroxyl, carbonyl, ether and/or carboxyl groups. ESI-FT-ICR-MS suggests that microbial compounds were largely aliphatic and richer in N than the background detrital material. Both molecular characterization tools suggest that CFE derived microbial biomass was largely lipid, carbohydrate and protein derived. SEC-HPLC-IRMS analysis revealed that 13C enrichment decreased with increasing MW of microbial compounds and the turnover time was deduced as 12.8 ± 0.6, 18.5 ± 0.6 and 22.9 ± 0.7 days for low, mid and high MW size classes, respectively. We conclude that low MW compounds represent the rapidly turned-over metabolite fraction of extractable soil microbial biomass consisting of organic acids, alcohols, amino acids and sugars; whereas, larger structural compounds are part of the cell envelope (likely membrane lipids, proteins or polysaccharides) with a much lower renewal rate. This relation of microbial carbon turnover to its molecular size, structure and composition thus highlights the significance of cellular biochemistry in determining the microbial contribution to soil carbon cycling and specifically soil organic matter formation

Identification of novel HIV-1 dependency factors in primary CCR4+CCR6+Th17 cells via a genome-wide transcriptional approach

Additional file 2: Table S2. The complete list of genes down regulated in Th17 versus Th1 subsets are included and classified based on p-values < 0.05 and FC cut-off 1.3

Les indicateurs de santé et d’expérience rapportés par les patients et les patientes. Conduire le changement pour l’amélioration des soins.

Demander aux personnes atteintes de maladies chroniques d’évaluer l’impact de leurs soins sur leur santé et leur expérience de soins primaires est l’étape la plus importante pour en rehausser la qualité. Le système de santé québécois collecte massivement des données médico-administratives qui peuvent fournir de l’information pertinente à l’orientation des politiques et à la mise en place de soins primaires pour répondre à leurs besoins. Cependant, peu d’information est disponible en ce qui a trait à la perception de ce public quant à leurs expériences de soins et leur santé. De plus, aucune collecte systématisée de données au niveau provincial ou canadien en santé primaire n’a été réalisée. La perspective du patient ou de la patiente est souvent absente des indicateurs collectés pour moduler les soins primaires et les politiques ministérielles. Pourtant, ces données sont essentielles à considérer pour : 1) la mise en place de plans d’intervention centrés sur leurs besoins réels; 2) l’établissement de politiques en santé en soins primaires, permettant une meilleure prise en charge de cette clientèle atteinte de maladies chroniques et; 3) pour l’amélioration (continue) des soins et services de santé. Notre équipe a été mandatée pour réaliser la portion québécoise de l’enquête internationale PaRIS (Patientreported Indicator Surveys) de l’Organisation de coopération et de développement économiques (OCDE), qui vise à dresser un portrait comparatif de l’expérience de soins et des résultats de santé de la patientèle atteinte de maladies chroniques bénéficiant de soins primaires. Afin de développer une méthodologie efficace et optimale, nous avons réalisé un colloque dans le cadre du 88e congrès de l’ACFAS, ayant eu lieu le 7 mai 2021. Celuici avait pour titre : Enjeux, priorités et leçons apprises pour les enquêtes sur la qualité des soins primaires selon la perspective des patients et des patientes. Nous avons réuni un groupe de personnes provenant de différents secteurs clés (patients et patientes partenaires, personnel de recherche et clinique, entreprises privées, experte en éthique, etc.) afin d’identifier les barrières et facilitants de l’implantation d’études de grande envergure et de s’inspirer des leçons apprises de celles-ci. Ce livre blanc vise à regrouper les constats tirés de cette journée ainsi que des recommandations formulées par l’équipe de co-responsables de la portion québécoise du PaRIS-OCDE.