The Essence of Participation Training: A Phenomenological Examination of Graduate Student Experiences

Data collected through interviews with individuals who recently experienced a Participation Training Institute reveals the nature of the phenomenon as reflective practice, as improvisational theater, and as eductive process. Comparative case studies might reveal productive similarities and differences between Participation Training and other forms of group learning

Intersecting Cultural Connections for Doctoral Persistence

This Roundtable Discussion addresses the barrier of informal peer support system (where its absence is one of the cited barriers to doctoral persistence) via the theoretical framework of shared case stories. These case stories explore the experiences of two women who entered a doctoral program as returning adults, and the ways in which their separate stories intersected and became interdependent, supporting their persistence towards degree. The presenters will provide a theater style format to share their stories in four scenes

O ENSINO TECNOLÓGICO NO BRASIL SOB A ÓTICA DE INSTITUIÇÕES PRIVADAS DE ENSINO.

As transformações em curso no Brasil, decorrentes das mudanças técnico-organizacionais no mundo do trabalho, estão fazendo ressurgir com muita ênfase, debates relativos a temas e problemas pertinentes às relações entre trabalho, qualificação e educação profissional. Novas propostas e práticas alternativas de formação profissional estão sendo geradas em diferentes espaços sociais por educadores, técnicos governamentais, pesquisadores, empresários, trabalhadores etc. Trata-se de um momento de criação e re-elaboração de várias concepções e projetos de formação profissional que retratam de forma diferenciada os interesses dos grupos sociais envolvidos nesses processos de transformação social. Este trabalho objetiva analisar criticamente os diferentes projetos e concepções de educação profissional que existentes no cenário atual

Single-cell whole-genome amplification technique impacts the accuracy of SNP microarray-based genotyping and copy number analyses

Methods of comprehensive microarray-based aneuploidy screening in single cells are rapidly emerging. Whole-genome amplification (WGA) remains a critical component for these methods to be successful. A number of commercially available WGA kits have been independently utilized in previous single-cell microarray studies. However, direct comparison of their performance on single cells has not been conducted. The present study demonstrates that among previously published methods, a single-cell GenomePlex WGA protocol provides the best combination of speed and accuracy for single nucleotide polymorphism microarray-based copy number (CN) analysis when compared with a REPLI-g- or GenomiPhi-based protocol. Alternatively, for applications that do not have constraints on turnaround time and that are directed at accurate genotyping rather than CN assignments, a REPLI-g-based protocol may provide the best solution

Methods for comprehensive chromosome screening of oocytes and embryos: capabilities, limitations, and evidence of validity

Preimplantation aneuploidy screening of cleavage stage embryos using fluorescence in situ hybridization (FISH) may no longer be considered the standard of care in reproductive medicine. Over the last few years, there has been considerable development of novel technologies for comprehensive chromosome screening (CCS) of the human genome. Among the notable methodologies that have been incorporated are whole genome amplification, metaphase and array based comparative genomic hybridization, single nucleotide polymorphism microarrays, and quantitative real-time PCR. As these methods become more integral to treating patients with infertility, it is critical that clinicians and scientists obtain a better understanding of their capabilities and limitations. This article will focus on reviewing these technologies and the evidence of their validity

Estilos de liderança: percepções sobre diferenças entre líderes homens e mulheres nas áreas de finanças e contabilidade

The purpose of this study is to identify how accounting and finance professionals perceive the leadership styles adopted by their male and female leaders. In order to analyze the hypothesis that there are differences in leadership styles between men and women, a bibliographical review of leadership style and contingency theories was carried out. For the data collection, an electronic survey was carried out, using an adapted version of the EAEG (Escala de Avaliação do Estilo Gerencial) scale proposed by Melo (2004), totaling 278 valid responses. Although the general hypothesis has been rejected, the results indicate that women still face differences in their chances of assuming leadership positions.O objetivo da pesquisa apresentada neste artigo é o de identificar a percepção dos profissionais das áreas de contabilidade e finanças acerca dos estilos de liderança adotados por seus líderes sendo homens ou mulheres. Para análise da hipótese de que existem diferenças de estilos de liderança entre homens e mulheres, foi realizada a revisão bibliográfica das teorias de liderança de estilo e contingenciais. Para a coleta de dados, foi realizada uma survey eletrônica, utilizando a escala EAEG (Escala de Avaliação do Estilo Gerencial), proposta por Melo (2004) com adaptações, totalizou 278 respostas válidas. Apesar da hipótese geral ter sido rejeitada, os resultados indicam que mulheres ainda enfrentam diferenciações quanto às suas possibilidades de assumir posições de liderança

Dispelling the Myths of Hunger: The Atlanta Community Food Bank and Collins Memorial UMC

Our group worked with the Atlanta Community Food Bank (ACFB) on their campaign to showcase the work of their partner agency Collins Memorial United Methodist Church (Collins Memorial UMC) through utilizing social media. We assisted ACFB in this endeavor by creating a short video which will be featured on the ACFB website and YouTube channel. We also created a sustainability plan booklet so that the project could be duplicated to showcase other partnerships. During the month of January, we researched the area of Bolton Road where distribution takes place and performed windshield and walking tours to have a deeper understanding of the need of fresh food supply in the community. Additionally, our student group volunteered to assist in distributing food to over 300 people weekly at the food pantry from the end of January 2013 through April 2013. On distribution days, we met with volunteers and clients and gained knowledge about the history of the area from formal and informal interviews. In April, we spent much of our time editing our final video and creating a sustainability plan. Our work with Collins Memorial UMC helped to identify the common interests of the community and key stakeholders: to end hunger and food insecurity. The utilization of social media will help the ACFB disseminate that message that hunger is a prevalent social issue which affects varying classes and populations, regardless of socioeconomic status, race, or gender, available to a wide audience

Effect of Exercise-Induced Reductions in Blood Volume on Cardiac Output and Oxygen Transport Capacity

We wanted to demonstrate the relationship between blood volume, cardiac size, cardiac output and maximum oxygen uptake ([Formula: see text] O(2max)) and to quantify blood volume shifts during exercise and their impact on oxygen transport. Twenty-four healthy, non-smoking, heterogeneously trained male participants (27 ± 4.6 years) performed incremental cycle ergometer tests to determine [Formula: see text] O(2max) and changes in blood volume and cardiac output. Cardiac output was determined by an inert gas rebreathing procedure. Heart dimensions were determined by 3D echocardiography. Blood volume and hemoglobin mass were determined by using the optimized CO-rebreathing method. The [Formula: see text] O(2max) ranged between 47.5 and 74.1 mL⋅kg(–1)⋅min(–1). Heart volume ranged between 7.7 and 17.9 mL⋅kg(–1) and maximum cardiac output ranged between 252 and 434 mL⋅kg(–1)⋅min(–1). The mean blood volume decreased by 8% (567 ± 187 mL, p = 0.001) until maximum exercise, leading to an increase in [Hb] by 1.3 ± 0.4 g⋅dL(–1) while peripheral oxygen saturation decreased by 6.1 ± 2.4%. There were close correlations between resting blood volume and heart volume (r = 0.73, p = 0.002), maximum blood volume and maximum cardiac output (r = 0.68, p = 0.001), and maximum cardiac output and [Formula: see text] O(2max) (r = 0.76, p < 0.001). An increase in maximum blood volume by 1,000 mL was associated with an increase in maximum stroke volume by 25 mL and in maximum cardiac output by 3.5 L⋅min(–1). In conclusion, blood volume markedly decreased until maximal exhaustion, potentially affecting the stroke volume response during exercise. Simultaneously, hemoconcentrations maintained the arterial oxygen content and compensated for the potential loss in maximum cardiac output. Therefore, a large blood volume at rest is an important factor for achieving a high cardiac output during exercise and blood volume shifts compensate for the decrease in peripheral oxygen saturation, thereby maintaining a high arteriovenous oxygen difference

Heat treatment of thioredoxin fusions increases the purity of α-helical transmembrane protein constructs.

Membrane proteins play key roles in cellular signaling and transport, represent the majority of drug targets, and are implicated in many diseases. Their relevance renders them important subjects for structural, biophysical, and functional investigations. However, obtaining membrane proteins in high purities is often challenging with conventional purification steps alone. To address this issue, we present here an approach to increase the purity of α-helical transmembrane proteins. Our approach exploits the Thioredoxin (Trx) tag system, which is able to confer some of its favorable properties, such as high solubility and thermostability, to its fusion partners. Using Trx fusions of transmembrane helical hairpin constructs derived from the human cystic fibrosis transmembrane conductance regulator (CFTR) and a bacterial ATP synthase, we establish conditions for the successful implementation of the selective heat treatment procedure to increase sample purity. We further examine systematically its efficacy with respect to different incubation times and temperatures using quantitative gel electrophoresis. We find that minute-timescale heat treatment of Trx-tagged fusion constructs with temperatures ranging from 50 to 90°C increases the purity of the membrane protein samples from ~60 to 98% even after affinity purification. We show that this single-step approach is even applicable in cases where regular selective heat purification from crude extracts, as reported for Trx fusions to soluble proteins, fails. Overall, our approach is easy to integrate into existing purification strategies and provides a facile route for increasing the purity of membrane protein constructs after purification by standard chromatography approaches