Operability analysis of control system for ROV launch-and-recovery from autonomous surface vessel

The launch and recovery of equipment such as remotely operated vehicles (ROVs) is a critical task that defines the operability limits of many marine operations. This paper considers the analysis of control systems that are designed to maximize the operability limits for launch and recovery of a ROV from a small unmanned surface vessel (USV). We use numerical simulation for the analysis, where the method combines recent approaches for wave compensating dynamic positioning, active heave compensation, and positioning control of the ROV with multi-body dynamic simulation of the surface vessel and ROV, including hydrodynamic forces and dynamic interactions from wires that depend on the ROV depth and moonpool. The results show that the choice of control algorithms and their tuning parameters has a significant effect on the system’s operability, and should be carefully designed and tuned to optimize the operability limits for any given sea state, weather and operational setup. The results show that numerical analysis with a system’s simulation is an effective tool to verify operability and its sensitivity to various parameters for the given ROV recovery application.publishedVersio

NovelRPL13Variants and Variable Clinical Expressivity in a Human Ribosomopathy With Spondyloepimetaphyseal Dysplasia

Spondyloepimetaphyseal dysplasias (SEMDs) are a heterogeneous group of disorders with variable growth failure and skeletal impairments affecting the spine and long bone epiphyses and metaphyses. Here we report on four unrelated families with SEMD in which we identified two monoallelic missense variants and one monoallelic splice site variant inRPL13, encoding the ribosomal protein eL13. In two out of four families, we observed autosomal dominant inheritance with incomplete penetrance and variable clinical expressivity; the phenotypes of the mutation-positive subjects ranged from normal height with or without hip dysplasia to severe SEMD with severe short stature and marked skeletal dysplasia.In vitrostudies on patient-derived dermal fibroblasts harboringRPL13missense mutations demonstrated normal eL13 expression, with proper subcellular localization but reduced colocalization with eL28 (p<0.001). Cellular functional defects in fibroblasts from mutation-positive subjects indicated a significant increase in the ratio of 60S subunits to 80S ribosomes (p= 0.007) and attenuated global translation (p= 0.017). In line with the human phenotype, ourrpl13mutant zebrafish model, generated by CRISPR-Cas9 editing, showed cartilage deformities at embryonic and juvenile stages. These findings extend the genetic spectrum ofRPL13mutations causing this novel human ribosomopathy with variable skeletal features. Our study underscores for the first time incomplete penetrance and broad phenotypic variability in SEMD-RPL13 type and confirms impaired ribosomal function. Furthermore, the newly generatedrpl13mutant zebrafish model corroborates the role of eL13 in skeletogenesis. (c) 2020 The Authors.Journal of Bone and Mineral Researchpublished by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR)..Peer reviewe

Ru(II)-diimine complexes and cytochrome P450 working hand-in-hand

With a growing interest in utilizing visible light to drive biocatalytic processes, several light-harvesting units and approaches have been employed to harness the synthetic potential of heme monooxygenases and carry out selective oxyfunctionalization of a wide range of substrates. While the fields of cytochrome P450 and Ru(II) photochemistry have separately been prolific, it is not until the turn of the 21st century that they converged. Non-covalent and subsequently covalently attached Ru(II) complexes were used to promote rapid intramolecular electron transfer in bacterial P450 enzymes. Photocatalytic activity with Ru(II)-modified P450 enzymes was achieved under reductive conditions with a judicious choice of a sacrificial electron donor. The initial concept of Ru(II)-modified P450 enzymes was further improved using protein engineering, photosensitizer functionalization and was successfully applied to other P450 enzymes. In this review, we wish to present the recent contributions from our group and others in utilizing Ru(II) complexes coupled with P450 enzymes in the broad context of photobiocatalysis, protein assemblies and chemoenzymatic reactions. The merging of chemical catalysts with the synthetic potential of P450 enzymes has led to the development of several chemoenzymatic approaches. Moreover, strained Ru(II) compounds have been shown to selectively inhibit P450 enzymes by releasing aromatic heterocycle containing molecules upon visible light excitation taking advantage of the rapid ligand loss feature in those complexes

Wege ins Institut – Wege aus dem Institut

In der Beilage zu dieser Ausgabe des HJK erläutern Studierende eine studentische Perspektive auf das Institut und das Studium und konzentrieren sich dabei auf die Themen Wissen und Wissensproduktion: Sie fragen einerseits danach, welches Wissen Studierende ins Studium mitbringen und welches Potenzial dieses im Laufe des Studiums noch entwickeln kann. Auf der anderen Seite erkunden sie, wie kulturanthropologische Forschung und Wissensproduktion in der Gesellschaft wirken. Die Autor*innen dieser Beilage befassen sich zudem bei der Frage nach Zukunftsfähigkeit mit der Funktion von wissenschaftlicher Sprache und ihrer Rolle bei Vermittlung von Wissen. Die Zugänglichkeit von Wissen sehen sie als essentiell für die Gestaltung der Gesellschaft und das positive Einwirken auf kritische Gegebenheiten und Missstände

Effectiveness of initiating biologics in severe asthma patients with high steroid exposure

Peer reviewedPostprin

Biomarker-defined clusters by level of Type 2 inflammatory involvement in severe asthma

Peer reviewedPostprin

Association between T2-related co-morbidities and effectiveness of biologics in severe asthma

Acknowledgments The authors thank Mr. Joash Tan (BSc, Hons), of the Observational and Pragmatic Research Institute (OPRI), and Ms Andrea Lim (BSc, Hons) of the Observational Pragmatic Research Institute (OPRI) for their editorial and formatting assistance that supported the development of this publication. Funding statement: This study was conducted by the Observational and Pragmatic Research Institute (OPRI) Pte Ltd and was partially funded by Optimum Patient Care Global and AstraZeneca Ltd. AstraZeneca UK LimitedPeer reviewe