Applications Of Monoclonal Antibodies To Studies Of Structure/function Relationships In Escherichia Coli F(1)-atpase

Twenty-one monoclonal antibodies to Escherichia coli F{dollar}\sb 1{dollar}-ATPase were characterized and used in structural studies of the enzyme. Solid phase competition assays placed antibodies recognizing the same or overlapping epitopes into competition subgroups. Immunoprecipitation and membrane-binding studies identified those antibodies recognizing external epitopes on soluble and membrane-bound forms of the holoenzyme and demonstrated that the {dollar}\delta{dollar} and {dollar}\epsilon{dollar} subunits are substantially exposed in F{dollar}\sb 1{dollar}F{dollar}\sb 0{dollar}.;Analysis of partially cleaved {dollar}\alpha{dollar} subunit using Western blots mapped antibody epitopes to its carboxyl-half. Analysis of CNBr/weak acid cleaved {dollar}\beta{dollar} located the non-inhibitory B-II antibody epitopes between Asp-345 and Met-380 and the inhibitory B-I antibody epitopes between Asp-381 and the carboxyl terminus. The {dollar}\epsilon{dollar}-binding site on {dollar}\beta{dollar} was mapped to the same region as the B-I antibodies. The Fab fragment of a B-I antibody and {dollar}\epsilon{dollar} were cross-linked indicating the proximity of their binding sites in native F{dollar}\sb 1{dollar}.;Cross-linking studies revealed neither differences in quaternary structure between soluble and membrane-bound F{dollar}\sb 1{dollar} nor any association between {dollar}\delta{dollar} or {dollar}\epsilon{dollar} and any F{dollar}\sb 0{dollar} subunit. Cross-linking studies of F{dollar}\sb 1{dollar} in the presence of the ATPase activators ethylene glycol and antibody {dollar}\epsilon{dollar}-4 examined the mechanism of {dollar}\epsilon{dollar}-inhibition. Ethylene glycol displaced {dollar}\epsilon{dollar} only from its site of 1-ethyl-3- (3-(dimethylamino)propyl) carbodiimide (EDC) cross-linkage to {dollar}\beta{dollar} demonstrating that {dollar}\epsilon{dollar}-inhibition is mediated through {dollar}\beta{dollar}. In contrast, antibody {dollar}\epsilon{dollar}-4 did not alter EDC cross-linking of {dollar}\epsilon{dollar} to {dollar}\beta{dollar} showing that this interaction is not always inhibitory. {dollar}\epsilon{dollar} could also be cross-linked to {dollar}\beta{dollar} by EDC in F{dollar}\sb 1{dollar}F{dollar}\sb 0{dollar} where it is not inhibitory. The inhibitory effect of {dollar}\epsilon{dollar} and the B-I antibodies binding to the carboxyl-terminal region of {dollar}\beta{dollar} region suggested a function for this region.;The antibodies were used to characterize an {dollar}\alpha{dollar}-{dollar}\delta{dollar} cross-link produced during column centrifugation of F{dollar}\sb 1{dollar} by oxidation of sulfhydryls. This cross-link affected neither the enzyme\u27s activity nor susceptibility to {dollar}\epsilon{dollar}-inhibition, casting doubt on rotational models of F{dollar}\sb 1{dollar} catalysis. The {dollar}\alpha{dollar}-{dollar}\delta{dollar} cross-link also resulted in a loss of binding affinity between F{dollar}\sb 1{dollar} and F{dollar}\sb 0{dollar}

The Association of Coloproctology of Great Britain and Ireland consensus guidelines in emergency colorectal surgery

ACKNOWLEDGEMENTS Review and editing: S.R. Brown, Professor of Surgery, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK. Email [email protected]. Patient summary: R.G. Arnott, Retired Professor, Patient Liaison Group, Association of Coloproctology of Great Britain and Ireland, Royal College of Surgeons of England, London, UK. Email [email protected]. Delphi review: C.P. Macklin. BMedSci BM BS FRCS DM, Consultant Colorectal Surgeon, Mid Yorkshire Hospitals, UK. Email [email protected] reviewedPublisher PD

Crop Updates 2010 - Genetically Modified Crops, Nutrition and Soils

GENETICALLY MODIFIED CROPS 1. Evaluation of the environmental and economic impact of Roundup Ready® canola in the Western Australian crop production system, James Fisher and Désirée Futures, York, Western Australia, Peter Tozer, PRT Consulting, Armidale NSW 2. Controlling wild radish (Raphanus raphanistrum) in Roundup Ready®1 Canola: Outcomes from the Nufarm 2009 Roundup Ready small plot trial Program, Mike Jackson, Nufarm Australia Limited 3. Weed strategies for glyphosate tolerant crops, John Moore, Department of Agriculture and Food 4. Results of the 2009 Western Australia Roundup Ready® canola trials, Dr James Neilsen, Canola Systems Specialist, Monsanto Australia NUTRITION 5. Modelling P runoff losses from agricultural systems, Geoff Anderson1, Wen Chen1, 2, Richard Bell2 and Ross Brennan1, 1Department of Agriculture and Food, 2School of Environmental Science, Murdoch University 6. Evaluation of deep banded biochars or biochar-mineral complex for increasing wheat yield or replacing fertiliser, Paul Blackwell, Allan Herbert and Stephen Davies, Department of Agriculture and Food 7. Improving fertiliser management: Redefining soil test-crop response relationships for canola in Western Australia, Wen Chen1, 2, Ross Brennan2, Richard Bell1, Mike Bolland2 and Geoff Anderson2, 1School of Environmental Science, Murdoch University, 2Department of Agriculture and Food 8. Phosphorus placement for wheat and lupins in WA cropping systems, 1School of Environmental Science, Murdoch University, 2Department of Agriculture and Food 9. The benefits of fertilising pastures for following crops, James Easton, Ryan Guthrie and Rowan Maddern, CSBP 10. Good nutrition produces high quality wheat in the high rainfall zone of Western Australia, Darren Hughes, Adveco Fertilisers; Wagga Wagga, Robert Belford, Curtin University of Technology, Wal Anderson, Department of Agriculture and Food, and Ian Edwards, Edstar Genetics, Perth 11. Lime to get maximum benefit from soil phosphorus, Reg Lunt and Bill Bowden, Department of Agriculture and Food 12. Variable rate top up N – Is it worth the trouble? Nigel Metz, South East Premium Wheat Growers Association (SEPWA) SOILS 13. Impact of soil inversion, soil dilution and claying on non-wetting sandplain soils, Stephen Davies, Peter Newman and Breanne Best, Department of Agriculture and Food 14. Long term effects of lime application on soil p, crop yields and annual ryegrass competition, Chris Gazey, Department of Agriculture and Food, Joel Andrew, Precision SoilTech, Belmont, Western Australi

A double-blind, placebo-controlled study of the short term effects of a spring water supplemented with magnesium bicarbonate on acid/base balance, bone metabolism and cardiovascular risk factors in postmenopausal women

<p>Abstract</p> <p>Background</p> <p>A number of health benefits including improvements in acid/base balance, bone metabolism, and cardiovascular risk factors have been attributed to the intake of magnesium rich alkaline mineral water. This study was designed to investigate the effects of the regular consumption of magnesium bicarbonate supplemented spring water on pH, biochemical parameters of bone metabolism, lipid profile and blood pressure in postmenopausal women.</p> <p>Findings</p> <p>In this double-blind, placebo-controlled, parallel-group, study, 67 postmenopausal women were randomised to receive between 1500 mL and 1800 mL daily of magnesium bicarbonate supplemented spring water (650 mg/L bicarbonate, 120 mg/L magnesium, pH 8.3-8.5) (supplemented water group) or spring water without supplements (control water group) over 84 days. Over this period biomarkers of bone turnover (serum parathyroid hormone (PTH), 1,25-dihydroxyvitamin D, osteocalcin, urinary telopeptides and hydroxyproline), serum lipids (total cholesterol, HDL-cholesterol, LDL-cholesterol and triglycerides), venous and urinary pH were measured together with measurements of standard biochemistry, haematology and urine examinations.</p> <p>Serum magnesium concentrations and urinary pH in subjects consuming the magnesium bicarbonate supplemented water increased significantly at Day 84 compared to subjects consuming the spring water control (magnesium - p = 0.03; pH - p = 0.018). The consumption of spring water led to a trend for an increase in parathyroid hormone (PTH) concentrations while the PTH concentrations remained stable with the intake of the supplemented spring water. However there were no significant effects of magnesium bicarbonate supplementation in changes to biomarkers of bone mineral metabolism (n-telopeptides, hydroxyproline, osteocalcin and 1,25-dihydroxyvitamin D) or serum lipids or blood pressure in postmenopausal women from Day 0 to Day 84.</p> <p>Conclusions</p> <p>Short term regular ingestion of magnesium bicarbonate supplemented water provides a source of orally available magnesium. Long term clinical studies are required to investigate any health benefits.</p> <p>Trial registration</p> <p>ACTRN12609000863235</p

A function-based typology for Earth’s ecosystems

As the United Nations develops a post-2020 global biodiversity framework for the Convention on Biological Diversity, attention is focusing on how new goals and targets for ecosystem conservation might serve its vision of ‘living in harmony with nature’(1,2). Advancing dual imperatives to conserve biodiversity and sustain ecosystem services requires reliable and resilient generalizations and predictions about ecosystem responses to environmental change and management(3). Ecosystems vary in their biota(4), service provision(5) and relative exposure to risks(6), yet there is no globally consistent classification of ecosystems that reflects functional responses to change and management. This hampers progress on developing conservation targets and sustainability goals. Here we present the International Union for Conservation of Nature (IUCN) Global Ecosystem Typology, a conceptually robust, scalable, spatially explicit approach for generalizations and predictions about functions, biota, risks and management remedies across the entire biosphere. The outcome of a major cross-disciplinary collaboration, this novel framework places all of Earth’s ecosystems into a unifying theoretical context to guide the transformation of ecosystem policy and management from global to local scales. This new information infrastructure will support knowledge transfer for ecosystem-specific management and restoration, globally standardized ecosystem risk assessments, natural capital accounting and progress on the post-2020 global biodiversity framework

Crop Updates 2005 - Farming Systems

This session covers forty four papers from different authors: PLENARY 1. 2005 Outlook, David Stephens and Nicola Telcik, Department of Agriculture FERTILITY AND NUTRITION 2. The effect of higher nitrogen fertiliser prices on rotation and fertiliser strategies in cropping systems, Ross Kingwell, Department of Agriculture and University of Western Australia 3. Stubble management: The short and long term implications for crop nutrition and soil fertility, Wayne Pluske, Nutrient Management Systems and Bill Bowden, Department of Agriculture 4. Stubble management: The pros and cons of different methods, Bill Bowden, Department of Agriculture, Western Australia and Mike Collins, WANTFA 5. Effect of stubble burning and seasonality on microbial processes and nutrient recycling, Frances Hoyle, The University of Western Australia 6. Soil biology and crop production in Western Australian farming systems, D.V. Murphy, N. Milton, M. Osman, F.C. Hoyle, L.K Abbott, W.R. Cookson and S. Darmawanto, The University of Western Australia 7. Urea is as effective as CAN when no rain for 10 days, Bill Crabtree, Crabtree Agricultural Consulting 8. Fertiliser (N,P,S,K) and lime requirements for wheat production in the Merredin district, Geoff Anderson, Department of Agriculture and Darren Kidson, Summit Fertilizers 9. Trace element applications: Up-front verses foliar? Bill Bowden and Ross Brennan, Department of Agriculture 10. Fertcare®, Environmental Product Stewardship and Advisor Standards for thee Fertiliser Industry, Nick Drew, Fertilizer Industry Federation of Australia (FIFA) SOIL AND LAND MANAGEMENT 11. Species response to row spacing, density and nutrition, Bill Bowden, Craig Scanlan, Lisa Sherriff, Bob French and Reg Lunt, Department of Agriculture 12. Investigation into the influence of row orientation in lupin crops, Jeff Russell, Department of Agriculture and Angie Roe, Farm Focus Consultants 13. Deriving variable rate management zones for crops, Ian Maling, Silverfox Solutions and Matthew Adams, DLI 14. In a world of Precision Agriculture, weigh trailers are not passé, Jeff Russell, Department of Agriculture 15. Cover crop management to combat ryegrass resistance and improve yields, Jeff Russell, Department of Agriculture and Angie Roe, Farm Focus Consultants 16. ARGT home page, the place to find information on annual ryegrass toxicity on the web, Dr George Yan, BART Pty Ltd 17. Shallow leading tine (SLT) ripper significantly reduces draft force, improves soil tilth and allows even distribution of subsoil ameliorants, Mohammad Hamza, Glen Riethmuller and Wal Anderson, Department of Agriculture PASTURE ANS SUMMER CROP SYSTEMS 18. New annual pasture legumes for Mediteranean farming systems, Angelo Loi, Phil Nichols, Clinton Revell and David Ferris, Department of Agriculture 19. How sustainable are phase rotations with Lucerne? Phil Ward, CSIRO Plant Industry 20. Management practicalities of summer cropping, Andrea Hills and Sally-Anne Penny, Department of Agriculture 21. Rainfall zone determines the effect of summer crops on winter yields, Andrea Hills, Sally-Anne Penny and David Hall, Department of Agriculture 22. Summer crops and water use, Andrea Hills, Sally-Anne Penny and David Hall, Department of Agriculture, and Michael Robertson and Don Gaydon, CSIRO Brisbane 23. Risk analysis of sorgum cropping, Andrea Hills and Sally-Anne Penny, Department of Agriculture, and Dr Michael Robertson and Don Gaydon, CSIRO Brisbane FARMER DECISION SUPPORT AND ADOPTION 24. Variety release and End Point Royalties – a new system? Tress Walmsley, Department of Agriculture 25. Farming system analaysis using the STEP Tool, Caroline Peek and Megan Abrahams, Department of Agriculture 26. The Leakage Calculator: A simple tool for groundwater recharge assessment, Paul Raper, Department of Agriculture 27. The cost of Salinity Calculator – your tool to assessing the profitability of salinity management options, Richard O’Donnell and Trevor Lacey, Department of Agriculture 28. Climate decision support tools, Meredith Fairbanks and David Tennant, Department of Agriculture 29. Horses for courses – using the best tools to manage climate risk, Cameron Weeks, Mingenew-Irwin Group/Planfarm and Richard Quinlan, Planfarm Agronomy 30. Use of seasonal outlook for making N decisions in Merredin, Meredith Fairbanks and Alexandra Edward, Department of Agriculture 31. Forecasts and profits, Benefits or bulldust? Chris Carter and Doug Hamilton, Department of Agriculture 32. A tool to estimate fixed and variable header and tractor depreciation costs, Peter Tozer, Department of Agriculture 33. Partners in grain: ‘Putting new faces in new places’, Renaye Horne, Department of Agriculture 34. Results from the Grower group Alliance, Tracey Gianatti, Grower Group Alliance 35. Local Farmer Group Network – farming systems research opportunities through local groups, Paul Carmody, Local Farmer Group Network GREENHOUSE GAS AND CLIMATE CHANGE 36. Changing rainfall patterns in the grainbelt, Ian Foster, Department of Agriculture 37. Vulnerability of broadscale agriculture to the impacts of climate change, Michele John, CSIRO (formerly Department of Agriculture) and Ross George, Department of Agriculture 38. Impacts of climate change on wheat yield at Merredin, Imma Farré and Ian Foster, Department of Agriculture 39. Climate change, land use suitability and water security, Ian Kininmonth, Dennis van Gool and Neil Coles, Department of Agriculture 40. Nitrous oxide emissions from cropping systems, Bill Porter, Department of Agriculture, Louise Barton, University of Western Australia 41. The potential of greenhouse sinks to underwrite improved land management in Western Australia, Richard Harper and Peter Ritson, CRC for Greenhouse Accounting and Forest Products Commission, Tony Beck, Tony Beck Consulting Services, Chris Mitchell and Michael Hill, CRC for Greenhouse Accounting 42. Removing uncertainty from greenhouse emissions, Fiona Barker-Reid, Will Gates, Ken Wilson and Rob Baigent, Department of Primary Industries - Victoria and CRC for Greenhouse Accounting (CRCGA), and Ian Galbally, Mick Meyer and Ian Weeks, CSIRO Atmospheric Research and CRCGA 43. Greenhouse in Agriculture Program (GIA), Traci Griffin, CRC for Greenhouse Accounting 44. Grains Greenhouse Accounting framework, D. Rodriguez, M. Probust, M. Meyers, D. Chen, A. Bennett, W. Strong, R. Nussey, I. Galbally and M. Howden CONTACT DETAILS FOR PRINCIPAL AUTHOR

Logging Affects Fledgling Sex Ratios and Baseline Corticosterone in a Forest Songbird

Silviculture (logging) creates a disturbance to forested environments. The degree to which forests are modified depends on the logging prescription and forest stand characteristics. In this study we compared the effects of two methods of group-selection (“moderate” and “heavy”) silviculture (GSS) and undisturbed reference stands on stress and offspring sex ratios of a forest interior species, the Ovenbird (Seiurus aurocapilla), in Algonquin Provincial Park, Canada. Blood samples were taken from nestlings for corticosterone and molecular sexing. We found that logging creates a disturbance that is stressful for nestling Ovenbirds, as illustrated by elevated baseline corticosterone in cut sites. Ovenbirds nesting in undisturbed reference forest produce fewer male offspring per brood (proportion male = 30%) while logging with progressively greater forest disturbance, shifted the offspring sex ratio towards males (proportion male: moderate = 50%, heavy = 70%). If Ovenbirds in undisturbed forests usually produce female-biased broods, then the production of males as a result of logging may disrupt population viability. We recommend a broad examination of nestling sex ratios in response to anthropogenic disturbance to determine the generality of our findings

Developing a core outcome set for fistulising perianal Crohn's disease

OBJECTIVE: Lack of standardised outcomes hampers effective analysis and comparison of data when comparing treatments in fistulising perianal Crohn's disease (pCD). Development of a standardised set of outcomes would resolve these issues. This study provides the definitive core outcome set (COS) for fistulising pCD. DESIGN: Candidate outcomes were generated through a systematic review and patient interviews. Consensus was established via a three-round Delphi process using a 9-point Likert scale based on how important they felt it was in determining treatment success culminating in a final consensus meeting. Stakeholders were recruited nationally and grouped into three panels (surgeons and radiologists, gastroenterologists and IBD specialist nurses, and patients). Participants received feedback fromtheir panel(in the second round) andall participants(in the third round) to allow refinement of their scores. RESULTS: A total of 295 outcomes were identified from systematic reviews and interviews that were categorised into 92 domains. 187 stakeholders (response rate 78.5%) prioritised 49 outcomes through a three-round Delphi study.The final consensus meeting of 41 experts and patients generated agreement on an eight domain COS. The COS comprised three patient-reported outcome domains (quality of life, incontinence and a combined score of patient priorities) and five clinician-reported outcome domains (perianal disease activity, development of new perianal abscess/sepsis, new/recurrent fistula, unplanned surgery and faecal diversion). CONCLUSION: A fistulising pCD COS has been produced by all key stakeholders. Application of the COS will reduce heterogeneity in outcome reporting, thereby facilitating more meaningful comparisons between treatments, data synthesis and ultimately benefit patient care