Innovative tools for nitrogen fertilization traceability in organic farming products: a cauliflower case study

Different research works have been carried out over the years to investigate new and reliable systems to test the authenticity of products obtained using organic cultivation methods. Based on a previously proposed integrated approach for discriminating organic from conventional products through the acquisition of isotopic data and other chemical and biochemical parameters, we herein report the results of an open-field cultivation case study for cauliflower crop. Experiments were carried out on soil, leaves, and corymb samples of cauliflowers grown using six different nitrogen fertilization treatments (organic, conventional, and mixed at different % of mineral fertilizers). The results of this study have shown that a multivariate analysis of isotopic data (13C/12C; 15N/14N, 34S/32S, 2H/1H, and 18O/16O isotopic ratios) combined with other parameters (fresh weight, total soluble solids, total acidity, cut resistance, CIE L*, a*, b* color indices, head height, head diameter, ascorbic acid content, total polyphenols, and ORAC units) performed using the linear discriminant analysis method gives researchers the possibility to discriminate organic products from conventional ones. Our study highlighted that the different isotopic signatures impressed on the cauliflowers by the different nitrogenous sources combined with the qualitative pattern of the crop, significantly affected by the different treatments, could effectively be jointly used to trace the organic origin of the cro

Impact on bone mineral density of tenofovir-containing HAART in HIV-1 infected children and adolescents: a report from 5 years of clinical experience

Purpose of the study Tenofovir disoproxil fumarate (TDF) is not approved for use in HIV-infected children (<18 years). In clinical practice a TAMs-sparing regimen may be needed. Use of TDF in children seems to be associated with decrease in bone mineral density that sometimes can stabilize after 24 weeks. The primary purpose was to characterized the change in bone mineral density (BMD), as measured by osteosonography (QUS), during and after treatment with tenofovir-containing HAART

'Omics' and chemical approaches used to monitor iron-deficiency in citrus rootstocks

Two different 'omics' approaches were performed to a better comprehension of biological mechanisms involved in citrus iron (Fe) deficiency. Tips roots from Swingle citrumelo and Carrizo citrange (sensitive and tolerant rootstocks, respectively), growing in pots with control and chlorotic soil, were used for transcriptomic and proteomic analysis. CombiMatrix array was performed to isolate differential genes, among which glutathione peroxidase (GPX), SAUR gene and glutamate dehydrogenase (GDH) showed to be the most involved ones. They were switched on Swingle grown on calcareous conditions compared to Carrizo (in the same soil) and to the same stock in the control soil. The over-expression of GPX could be the effort of plants to neutralize the oxidative environment produced by stress. The involvement of auxin (and as consequence of SAUR gene) in the regulation of Fe deficiency responses is also well known. Both genes were considered in association to peroxidase, ferric chelate reductase activities, iron and chlorophyll content, to monitor the degree of suffering of rootstocks. Among differentially expressed proteins, identified by means of 2D-PAGE and RP-HPLC/nESI-MSMS, a strong down-regulation of cytosolic pyrophosphate-dependent phosphofructokinase β-subunit and NADPH-isocitrate dehydrogenase could produce plant inability to sustain the energetic request of cell roots

Soil quality comparison among organically and conventionally managed citrus orchards in Eastern Sicily (Italy)

The aim of this work was to compare soil fertility status of conventional and organic managed citrus orchards, using specific soil system descriptors. The research was carried out in a Mediterranean environment (Sicily region, Southern Italy), on Navelina and Tarocco orange orchards. Soil characteristics were analysed in 54 farms under both organic and conventional management. Farms were selected to obtain similar pairs (27) in the same environmental conditions. For each soil, total organic carbon, total nitrogen, mineral NO3-N and NH4-N were determined. In addition, in order to evaluate biological fertility status of soils, carbon mineralisation and nitrogen mineralisation in anaerobic conditions were studied. Soils humic fraction was at least characterised qualitatively by isoelectric focusing technique, to obtain information on soil organic matter stability. Results obtained attest that the organic citrus orchards soils are able to conserve energy and store nutrients more than the conventional ones

Quality of cold stored lemon fruit from orchards consociated to ancient olive trees

In the hilly area of Gioia Tauro (Calabria, Southern Italy), lemon orchards are grown in consociation with centuries-old olive trees. Lemons are partially shaded by olive canopies and the microclimate at the level of their canopies is suitable for plants growth and quality productions. Under these conditions, lemon trees are grown even without irrigation, providing, despite this limitation, a quality product. This study aimed to i) investigate the qualitative characterisation of two clonal selections of the lemon cultivar Femminello, F. Siracusano (S) and F. Zagara bianca (ZB), from the described intercropping, on irrigated (I) and non-irrigated (NI) crops; ii) assess the quality preservation during cold storage, in order to evaluate the availability of lemons for marketing in a period of shortage such as the summer season. Fruits were harvested at commercial maturity, and cold stored at 10±1°C and RH 85-90%, for 60 days. Decay incidence, physiological disorders, weight loss, and the main physical-chemical parameters were assessed at harvest (T0) and every 15 days (T15, T30, T45, T60). The absence of decay and physiological disorders was observed throughout the 60-day storage period, in both clonal selections under the two management conditions. The weight loss was greater in fruits from irrigated lemon groves of both S and ZB. S_I showed significantly lower fruits weight and higher titratable acidity than S_NI. Total soluble solids and titratable acidity were statistically lower for ZB_I than for NI fruits

Assessment of soil quality in wetlands in Eastern Sicily

Wetlands are of great importance for biodiversity as they support a rich variety of plant and animal species, and the presence of endemic and migratory species is frequent. It is therefore necessary to protect and monitor such areas. The work conducted, reported in the present paper, aimed to verify the quality of soil in most wetlands in eastern Sicily. Specifically, four areas were studied: three Oriented Nature Reserves (RNO), the Saline di Priolo, with the adjacent Penisola di Magnisi, the Saline di Siracusa, and the Faunistic Oasis of Vendicari11 The Oasis takes the overall name of Vendicari, in reality it is made up of various localities that take on different names: Vendicari, Marianelli, Cittadella, Calamosche., and one Special Protection Area (SPA), the Biviere di Lentini. The study areas were sampled in 12 different sites, in spring and in autumn, to monitor soil fauna and assess the soil biological quality through the calculation of the QBS-ar. The results represent the first data relating to the QBS-ar in Sicily and, in particular, relating to wetlands.The QBS-ar values recorded were all higher, for both seasons, than the averages reported for land use by Menta et al., 2018. The statistical analysis regarding annual QBS-ar divides the sites into two groups: Saline di Priolo1, Marianelli1, Cittadella1, Vendicari1, Biviere di Lentini2 with a QBS-ar > 200 and Biviere di Lentini1, Saline di Priolo2, Penisola di Magnisi1, Penisola di Magnisi2, Saline di Siracusa1 and Saline di Siracusa2 with QBS-ar < 200; Calamosche1 site show no significant difference between the two groups. Although the number of individuals varied considerably in the two seasons, there are no seasonal differences in the number of taxa and in the QBS-ar.The chemical-physical parameters that influenced the fauna composition and the QBS-ar were mostly those related to the soil texture; some groups were positively correlated with the percentage of silt, while others were negatively correlated with the percentage of sand. The QBS-ar show the same correlations with silt and sand. Proximity or non-proximity to water did not determine variations in the fauna composition and in the QBS-ar values. Soil salinity appeared to be a factor influencing the fauna composition, but further studies are needed to confirm such relief

Impact on bone mineral density of tenofovir-containing HAART in HIV-1 infected children and adolescents: a report from 5 years of clinical experience

Purpose of the study Tenofovir disoproxil fumarate (TDF) is not approved for use in HIV-infected children (<18 years). In clinical practice a TAMs-sparing regimen may be needed. Use of TDF in children seems to be associated with decrease in bone mineral density that sometimes can stabilize after 24 weeks. The primary purpose was to characterized the change in bone mineral density (BMD), as measured by osteosonography (QUS), during and after treatment with tenofovir-containing HAART

Adaptation of citrus orchards to deficit irrigation strategies

In this study, the adaptation characteristics of orange trees, related to the application over a decade of deficit irrigation (DI) strategies, have been explored. To this purpose, the analysis of a minimal dataset composed of physiological information (stem water potential - Ψ and sap flow - SF measurements), yield (fruits number and weight) and qualitative parameters (titratable acidity, TA; and total soluble solids, TSS) was performed with reference to the last irrigation seasons (i.e. 2018–19). The applied irrigation treatments were the following: sustained deficit irrigation (SDI); regulated deficit irrigation (RDI); partial root-zone drying (PRD), each distributing a water deficit of about 19%, 29% and 52%, respectively, compared to the control treatment (FI) supplying the full irrigation level (100% ET). In general, higher water use efficiencies (WUE) have been obtained in DI treatments, which guarantee greater water savings (up to 50%), without affecting yield and quality characteristics. In particular, the most stressed treatment (PRD), while reaching the lowest Ψ values (− 1.8 to − 2.0 MPa), as also shown by SF versus Ψ clusters, resulted in WUE values for yield (WUE), TA (WUE) and TSS (WUE) parameters of approximately 2.6, 2.9, and 3.1 times greater than FI, respectively. Overall, this study allowed identifying the cumulative adaptation characteristics of the orange trees under study to the application of long-term DI strategies and showing that trees were able to achieve yields and qualitative features similar to those obtained with FI, even after 10 years of application of deficient irrigation regimes.The work was carried out in the frame of Programma Operativo Nazionale (PON) “Attraction and International Mobility” (AIM) 1848200-2 initiative (D.V.) and within the project “Strategie per migliorare l’efficienza d’uso dell’acqua per le colture mediterranee” (SaveIrriWater) Linea 2 Ricerca di Ateneo 2020–22 (Universit` a degli Studi di Catania). J.M.R.-C. acknowledges the postdoctoral financial support received from Juan de la Cierva Spanish Postdoctoral Program (FJC2018-037196-I

Early defoliation effects on water status, fruit yield and must quality of ‘Nerello mascalese’ grapes

The effect of basal zone defoliation on vines influences yield and ripening depending on the phenological stage and its intensity. Early basal defoliation (EBD) was carried out at flowering on the autochthonous Vitis vinifera (L.) cultivar ‘Nerello mascalese’ grown on the eastern slopes of Mount Etna, Sicily. The effects were evaluated over a two-year period. In the first year, the canopy retained 67 % of its original leaf area after EBD and in the second year, 58 %. Compared with control vines, mid-day leaf water potentials in the EBD vines enjoyed higher water status throughout the growing seasons in both years. EBD had no significant negative effects on yield but did have significant positive effects on the levels of total polyphenols, total flavonols and total anthocyanins. Yield and its components are conditioned by a number of factors mainly linked to climate and to the vines’ general condition during berry growth and ripening. Even after two consecutive years of treatment, EBD does not have any negative effects on yield. However, EBD does have strong positive effects on berry quality attributes as a consequence of the following: greater exposure of the cluster to sunlight, higher vine water status and avoidance of high temperature stresses in the last phase of ripening during the analysis of anthocyanin

Effects of Different Inter-Row Soil Management and Intra-Row Living Mulch on Spontaneous Flora, Beneficial Insects, and Growth of Young Olive Trees in Southern Italy

Conservation agriculture (i.e., minimized soil disturbance and permanent soil covering) and living mulches represent two agroecological practices that can improve soil fertility, spontaneous flora, and beneficial insect communities. This research studied the effect of these practices in a young olive orchard in the Mediterranean area. Two Sicilian olive cultivars (&lsquo;Nocellara del Belice&rsquo; and &lsquo;Nocellara etnea&rsquo;) were used for the field experiment; inter-row minimum and zero tillage and four species of aromatic plants as living mulch along the row were tested. Spontaneous flora and beneficial insect communities, as well as tree growth, were monitored. The inter-row management did not influence the spontaneous flora dynamics. The species adopted for living mulch showed a very different degree of development and soil cover; 69 insect species (pollinators and predators) belonging to five orders (Hymenoptera, Lepidoptera, Diptera, Neuroptera, and Coleoptera) and 17 families were recorded. The growth of the olive trees was not affected by the conservative strategies.: In the inter-row, the growth of the spontaneous flora was limited by the high temperatures during the summer. Among the living mulch species, sage and lemongrass guaranteed an almost full soil cover, reducing the need for weed management along the row, as well as increasing the beneficial insects without influencing the young tree growth