Genes for asparagine metabolism in Lotus japonicus : differential expression and interconnection with photorespiration

Background: Asparagine is a very important nitrogen transport and storage compound in plants due to its high nitrogen/carbon ratio and stability. Asparagine intracellu lar concentration depends on a balance between asparagine biosynthesis and degradation. The main enzymes involved in asparagine metabolism are as paragine synthetase (ASN), asparaginase (NSE) and serine-glyoxylate aminotransfera se (SGAT). The study of the genes encoding for these enzymes in the model legume Lotus japonicus is of particular interest since it has been proposed that asparagine is the principal molecule used to transport reduced nitrogen within the plant in most temperate legumes. Results: A differential expression of genes encoding for seve ral enzymes involved in asparagine metabolism was detected in L. japonicus . ASN is encoded by three genes, LjASN1 was the most highly expressed in mature leaves while LjASN2 expression was negligible and LjASN3 showed a low expression in this organ, suggesting that LjASN1 is the main gene responsible for asparagine synthesis in mature leaves. In young leaves, LjASN3 was the only ASN gene expressed although at low levels, while all the three genes encoding for NSE were highly expressed, especially LjNSE1 .Innodules, LjASN2 and LjNSE2 were the most highly expressed genes, suggesting an important role for these genes in this organ. Several lines of evidence support the connection between asparagine metabolic genes and photorespiration in L. japonicus : a) a mutant plant deficient in LjNSE1 showed a dramatic decrease in the expression of the two genes encoding for SGAT; b) expression of the genes involved in asparagine metabolism is altered in a photorespiratory mutant lacking plastidic glutamine synthetase; c) a clustering analysis indicated a similar pattern of expression among several genes involved in photorespiratory and asparagine metabolism, indicating a clear link between LjASN1 and LjSGAT genes and photorespiration. Conclusions: The results obtained in this paper indicate the exis tence of a differential expression of asparagine metabolic genes in L. japonicus and point out the crucial relevance of particular genes in different organs. Moreover, the data presented establish clear links betw een asparagine and photorespiratory metabolic genes in this plant.Junta de Andalucía (P10-CVI- 6368)FEDER-Ministerio de Economía y Competitividad (AGL 2014 – 54413-R

CARACTERÍSTICAS DE LAS COMPENSACIONES DE LOS HOTELES PYMES DE VILLAVICENCIO, COLOMBIA - Characteristics of the salary compensation of the pymes hotels of Villavicencio, Colombia.

Los procesos de gestión humana han permitido mejorar las condiciones laborales de los colaboradores, siendo el proceso de compensación salarial un factor clave a la hora de motivar los colaboradores. Por eso, el proyecto hace parte del estudio salarial de la ciudad de Villavicencio, Meta, realizado por el grupo GYDO de la Universidad de los Llanos, donde se analizaron los programas de compensación salarial en los hoteles pymes de Villavicencio, además de identificar las condiciones de contratación de personal, analizar las características salariales y determinar cómo son los sistemas de incentivos y aumentos salariales. Se realizó mediante un estudio descriptivo con un enfoque cuantitativo y con el apoyo de los cuestionarios MP05a y MP05b. En los resultados se evidenció la importancia que le dan los hoteles a las compensaciones salariales, donde el tipo de los incentivos y los beneficios sociales resultan ser fundamentales al momento de motivar a los colaboradores, además se logró conocer las características más importantes que tienen los diferentes cargos que hay en los hoteles. Por lo tanto, los hoteles pymes de la ciudad de Villavicencio requieren de mejoras en este proceso, principalmente en la implementación de las nuevas tendencias salariales, como lo es la aplicación del salario emocional, siendo éste un término poco conocido por el sector

Estudio de las causas y efectos en la aplicación del juego para el desarrollo del pensamiento en los niños y niñas de los segundos y terceros años de básica de las escuelas Nuestra Señora de Fátima, Ciudad de Ibarra y Guayaquil de Alpachaca de la ciudad de Ibarra

Investigar las causas y efectos de las actividades lúdicas en el proceso de enseñanza - aprendizaje de acuerdo a los principios aportados por Piaget, que favorezcan el desarrollo del pensamiento en los niños y niñas de los segundos y terceros años de educación básica de las escuelas “Nuestra Señora de Fátima”, “Ciudad de Ibarra” y “Guayaquil de Alpachaca” del cantón Ibarra.La presente investigación trata sobre las causas y efectos de las actividades lúdicas en el proceso de enseñanza aprendizaje de acuerdo a los principios aportados por Piaget que favorecen el desarrollo dl pensamiento de los niños y niñas, diagnosticar las causas y efectos de las actividades lúdicas que utilizan los docentes de las escuelas, elaborar una propuesta de un manual de actividades lúdicas para el desarrollo del pensamiento

Enfermedad pulmonar intersticial: silicosis, reporte de un caso

Varón de 51 años de edad que acudió por presentar tos seca y dolor torácico desde hace más de 10 años. Tiene antecedentes de exposición a biomasa, ha trabajado como supervisor de construcción de túneles. Hace 12 años presentó cuadro de hemoptisis por el cual recibió tratamiento antituberculoso a pesar de no contar con baciloscopía positiva. El examen físico fue normal excepto que se encontraron crepitantes finos en ambas bases. Presentó patrón obstructivo en la espirometría. Y se evidenció gran compromiso intersticial en el parénquima pulmonar en los estudios de tomografía. Se le practicó una biopsia por video toracoscopía, la cual confirmó del diagnóstico de silicosis

Damage Detection in Metallic Beams from Dynamic Strain Measurements under Different Load Cases by Using Automatic Clustering and Pattern Recognition Techniques

International audienceIn general, the change in the local strain field or global stiffness caused by damage in a structure is very small and the strain field tends to homogenize very quickly in the field close to the defect. Moreover, other environmental effects can fade the slight changes in the strain field. Only by comparing the response of the structure at several points some information about damage may be unveiled. By means of pattern recognition techniques based on the strain field, this task can be achieved. This is the basis of the strain measurements data-driven models. The main limitation of the strain field pattern recognition techniques lies in the susceptibility of the strain field to change depending on the load conditions. In the case of dynamic loads, this may reflect even a greater limitation. Robust automated techniques are required to manage these limitations. In first instance, automatic clustering techniques are needed so that data can be classified according to the load conditions and secondly, a dimensional reduction technique is needed in order to obtain patterns that often underlie from data. Within the context of this paper, a combination of Local Density-based Simultaneous Two-Level (DS2L-SOM) Clustering based on Self-Organizing Maps (SOM) and Principal Components Analysis (PCA) is proposed in order to firstly, classify load conditions and secondly, perform strain field pattern recognition. The clustering technique is the basis for an Optimal Baseline Selection. An experimental validation of the technique is discussed in this paper, comparing damages of different sizes and positions in an aluminum beam, under a set of combined loads under dynamic conditions. Strains were measured at several points by using Fiber Bragg Gratings

Influence of diet in urinary levels of metals in a biomonitoring study of a child population of the Valencian region (Spain)

[EN] Pollution by trace elements and its possible effect on organisms has become a worldwide concern due to the increasing presence of trace elements in the environment and especially in the food chain. Exposure to chemicals has traditionally been measured using environmental samples, however, human biomonitoring brings a different perspective, in which all sources and exposure pathways are integrated. The objective of this paper is to discern the possible relationship between children's diet and the metals found in children urine. With this aim in mind, a total of 120 voluntaries participated in a diet survey carried out in a school-aged population (age 6-11) from the Valencian region. In addition, twenty trace elements were analysed in children urine (arsenic, antimony, barium, beryllium, caesium, cadmium, cobalt, copper, lead, manganese, mercury, molybdenum, nickel, platinum, selenium, thallium, thorium, uranium, vanadium and zinc). Results permitted to compare metal levels in urine with metal levels of other biomonitoring studies to conclude that values, including ours, were similar in most studies. On the other hand, children who ate more vegetables had the highest values in cadmium, copper, molybdenum, antimony, thallium, vanadium, and zinc, while those who ate more fish reached higher values in mercury. Finally, children who ate more cereals and baked products had higher values in total arsenic. (c) 2017 Elsevier B.V. All rights reserved.This study was part of the research project CIPAV supported by the Conselleria de Educacion, Generalitat Valenciana (GV/2011/007).Pérez-Torres, R.; Doménech Antich, EM.; Conchado Peiró, A.; Sanchez, A.; Coscollà, C.; Yusà, V. (2018). Influence of diet in urinary levels of metals in a biomonitoring study of a child population of the Valencian region (Spain). The Science of The Total Environment. 618:1647-1657. https://doi.org/10.1016/j.scitotenv.2017.10.011S1647165761

LOS HELECHOS Y PLANTAS AFINES DEL BOSQUE MESÓFILO DE MONTAÑA DE BANDERILLA, VERACRUZ, MÉXICO

Our inventory of ferns and similar plantspresent in Banderilla, Veracruz, Mexico,focused on cloud forest relicts. We report130 species belonging to 24 families and 49genera of such plants in Banderilla,representing about 21% of the pteridophyteflora of the state. This species richness isremarkable considering the small size of themunicipality (22.21km2 or 0.03% of the areaof the state).Se realizó el inventario de los helechos ylas plantas afines presentes en Banderilla,Veracruz, México, especialmente en losrelictos de bosque mesófilo de montaña.Se registran 130 especies pertenecientes a24 familias y 49 géneros, que representancerca del 21% de los Pteridófitos delestado. Esta riqueza es destacable considerando que el municipio tiene unasuperficie de 22.21 km2 (0.03% del total dela superficie estatal)

A Pattern Recognition Approach for Damage Detection and Temperature Compensation in Acousto-Ultrasonics

International audienceThe global trends in the construction of modern structures require the integration of sensors together with data recording and analysis modules so that their integrity can be continuously monitored for safe-life, economic and ecological reasons. This process of measuring and analysing the data from a distributed sensor network all over a structural system in order to quantify its condition is known as structural health monitoring (SHM). Guided ultrasonic wave-based techniques are increasingly being adapted and used in several SHM systems which benefit from built&#8208,in transduction, large inspection ranges, and high sensitivity to small flaws. However, for reliable health monitoring, much information regarding the innate characteristics of the sources and their propagation is essential. Moreover, any SHM system which is expected to transition to field operation must take into account the influence of environmental and operational changes which cause modifications in the stiffness and damping of the structure and consequently modify its dynamic behaviour. On that account, special attention is paid in this paper to the development of an efficient SHM methodology where robust signal processing and pattern recognition techniques are integrated for the correct interpretation of complex ultrasonic waves within the context of damage detection and identification. The methodology is based on an acousto-ultrasonics technique where the discrete wavelet transform is evaluated for feature extraction and selection, linear principal component analysis for data-driven modelling and self-organizing maps for a two-level clustering under the principle of local density. At the end, the methodology is experimentally demonstrated and results show that all the damages were detectable and i

Inducción y capacitación como factor de desarrollo de los IPS (Centros Médicos) de Villavicencio, Meta - Colombia

The training (induction and training) that the collaborators of the clinics of Villavicencio live is a practice that contributes to the organizational development but when not doing it could affect other processes, for thatreason with the objective of describing the factors that intervene in this practice, conducted a descriptive investigation to the IPS (Medical Centers) of the city, through the instrument Mp03 Induction and Training of the GYDO Group, applied to those in charge of human management, characterizing the practice considered of great importance as a strategy of organizational and personal improvement, These organizations have formalized training plans and consider training as a competitive advantage that allows them to offer a quality service.El entrenamiento (inducción y capacitación) que viven los colaboradores de las clí­nicas de Villavicencio es una práctica que aporta al desarrollo organizacional pero al no hacerse efectivamente podrí­a afectar otros procesos, por ello con el objetivo de describir los factores que intervienen en esta práctica, se realizó una investigación descriptiva a las IPS (Centros Médicos) de la ciudad, a través del instrumento Mp03 Inducción y Capacitación del Grupo GYDO, aplicado a los encargados de gestión humana, caracterizando la práctica considerada de gran importancia como estrategia de mejora organizacional y personal, estas organizaciones cuentan con planes formalizados de entrenamiento y consideran las capacitaciones como una ventaja competitiva que les permite ofrecer un servicio de calidad. Palabras clave: Centros Médicos, inducción, capacitación, entrenamiento, gestión humana. Abstract: The training (induction and training) that the collaborators of the clinics of Villavicencio live is a practice that contributes to the organizational development but when not doing it could affect other processes, for that reason with the objective of describing the factors that intervene in this practice, conducted a descriptive investigation to the IPS (Medical Centers) of the city, through the instrument Mp03 Induction and Training of the GYDO Group, applied to those in charge of human management, characterizing the practice considered of great importance as a strategy of organizational and personal improvement, These organizations have formalized training plans and consider training as a competitive advantage that allows them to offer a quality service. Keywords: Medical Centers, induction, training, human management.

Inducción y capacitación como factor de desarrollo de los IPS (Centros Médicos) de Villavicencio, Meta - Colombia

The training (induction and training) that the collaborators of the clinics of Villavicencio live is a practice that contributes to the organizational development but when not doing it could affect other processes, for thatreason with the objective of describing the factors that intervene in this practice, conducted a descriptive investigation to the IPS (Medical Centers) of the city, through the instrument Mp03 Induction and Training of the GYDO Group, applied to those in charge of human management, characterizing the practice considered of great importance as a strategy of organizational and personal improvement, These organizations have formalized training plans and consider training as a competitive advantage that allows them to offer a quality service.El entrenamiento (inducción y capacitación) que viven los colaboradores de las clí­nicas de Villavicencio es una práctica que aporta al desarrollo organizacional pero al no hacerse efectivamente podrí­a afectar otros procesos, por ello con el objetivo de describir los factores que intervienen en esta práctica, se realizó una investigación descriptiva a las IPS (Centros Médicos) de la ciudad, a través del instrumento Mp03 Inducción y Capacitación del Grupo GYDO, aplicado a los encargados de gestión humana, caracterizando la práctica considerada de gran importancia como estrategia de mejora organizacional y personal, estas organizaciones cuentan con planes formalizados de entrenamiento y consideran las capacitaciones como una ventaja competitiva que les permite ofrecer un servicio de calidad. Palabras clave: Centros Médicos, inducción, capacitación, entrenamiento, gestión humana. Abstract: The training (induction and training) that the collaborators of the clinics of Villavicencio live is a practice that contributes to the organizational development but when not doing it could affect other processes, for that reason with the objective of describing the factors that intervene in this practice, conducted a descriptive investigation to the IPS (Medical Centers) of the city, through the instrument Mp03 Induction and Training of the GYDO Group, applied to those in charge of human management, characterizing the practice considered of great importance as a strategy of organizational and personal improvement, These organizations have formalized training plans and consider training as a competitive advantage that allows them to offer a quality service. Keywords: Medical Centers, induction, training, human management.