271 research outputs found
Robot Egomotion from the Deformation of Active Contours
Traditional sources of information for image-based computer vision algorithms have been points, lines, corners, and recently SIFT features (Lowe, 2004), which seem to represent at present the state of the art in feature definition. Alternatively, the present work explores the possibility of using tracked contours as informative features, especially in applications no
Impact of solid-electrolyte interphase reformation on capacity loss in silicon-based lithium-ion batteries
High-density silicon composite anodes show large volume changes upon charging/discharging triggering the reformation of the solid electrolyte interface (SEI), an interface initially formed at the silicon surface. The question remains how the reformation process and accompanied material evolution, in particular for industrial up-scalable cells, impacts cell performance. Here, we develop a correlated workflow incorporating X-ray microscopy, field-emission scanning electron microscopy tomography, elemental imaging and deep learning-based microstructure quantification suitable to witness the structural and chemical progression of the silicon and SEI reformation upon cycling. The nanometer-sized SEI layer evolves into a micron-sized silicon electrolyte composite structure at prolonged cycles. Experimental-informed electrochemical modelling endorses an underutilisation of the active material due to the silicon electrolyte composite growth affecting the capacity. A chemo-mechanical model is used to analyse the stability of the SEI/silicon reaction front and to investigate the effects of material properties on the stability that can affect the capacity loss
Environment and Rural Affairs Monitoring & Modelling Programme - ERAMMP Document-76: Field-Survey Handbook (Procedures) Pollinators 2021
CALHM1-Mediated ATP Release and Ciliary Beat Frequency Modulation in Nasal Epithelial Cells
Mechanical stimulation of airway epithelial cells causes apical release of ATP, which increases ciliary beat frequency (CBF) and speeds up mucociliary clearance. The mechanisms responsible for this ATP release are poorly understood. CALHM1, a transmembrane protein with shared structural features to connexins and pannexins, has been implicated in ATP release from taste buds, but it has not been evaluated for a functional role in the airway. In the present study, Calhm1 knockout, Panx1 knockout, and wild-type mouse nasal septal epithelial cells were grown at an air-liquid interface (ALI) and subjected to light mechanical stimulation from an air puff. Apical ATP release was attenuated in Calhm1 knockout cultures following mechanical stimulation at a pressure of 55 mmHg for 50 milliseconds (p \u3c 0.05). Addition of carbenoxolone, a PANX1 channel blocker, completely abolished ATP release in Calhm1 knockout cultures but not in wild type or Panx1 knockout cultures. An increase in CBF was observed in wild-type ALIs following mechanical stimulation, and this increase was significantly lower (p \u3c 0.01) in Calhm1 knockout cultures. These results demonstrate that CALHM1 plays a newly defined role, complementary to PANX1, in ATP release and downstream CBF modulation following a mechanical stimulus in airway epithelial cells. © 2017 The Author(s)
T1R3: A human calcium taste receptor
Many animals can detect the taste of calcium but it is unclear how or whether humans have this ability. We show here that calcium activates hTAS1R3-transfected HEK293 cells and that this response is attenuated by lactisole, an inhibitor of hT1R3. Moreover, trained volunteers report that lactisole reduces the calcium intensity of calcium lactate. Thus, humans can detect calcium by taste, T1R3 is a receptor responsible for this, and lactisole can reduce the taste perception of calcium by acting on T1R3
Effect of Chorda Tympani Nerve Transection on Salt Taste Perception in Mice
Effects of gustatory nerve transection on salt taste have been studied extensively in rats and hamsters but have not been well explored in the mouse. We examined the effects of chorda tympani (CT) nerve transection on NaCl taste preferences and thresholds in outbred CD-1 mice using a high-throughput phenotyping method developed in our laboratory. To measure taste thresholds, mice were conditioned by oral self-administration of LiCl or NaCl and then presented with NaCl concentration series in 2-bottle preference tests. LiCl-conditioned and control NaCl-exposed mice were given bilateral transections of the CT nerve (LiCl-CTX, NaCl-CTX) or were left intact as controls (LiCl-CNT, NaCl-CNT). After recovery from surgery, mice received a concentration series of NaCl (0–300 mM) in 48-h 2-bottle tests. CT transection increased NaCl taste thresholds in LiCl-conditioned mice and eliminated avoidance of concentrated NaCl in control NaCl-exposed mice. This demonstrates that in mice, the CT nerve is important for detection and recognition of NaCl taste and is necessary for the normal avoidance of high concentrations of NaCl. The results of this experiment also show that the method of high-throughput phenotyping of salt taste thresholds is suitable for detecting changes in the taste periphery in mouse genetic studies
Exploring relationships between land use intensity, habitat heterogeneity and biodiversity to identify and monitor areas of High Nature Value farming
Understanding how species richness is distributed across landscapes and which variables may be used as predictors is important for spatially targeting management interventions. This study uses finely resolved data over a large geographical area to explore relationships between land-use intensity, habitat heterogeneity and species richness of multiple taxa. It aims to identify surrogate landscape metrics, valid for a range of taxa, which can be used to map and monitor High Nature Value farmland (HNV).
Results show that variation in species richness is distributed along two axes: land-use intensity and habitat heterogeneity. At low intensity land-use, species rich groups include wetland plants, plant habitat indicators, upland birds and rare invertebrates, whilst richness of other species groups (farmland birds, butterflies, bees) was associated with higher land-use intensity. Habitat heterogeneity (broadleaved woodland connectivity, hedgerows, habitat diversity) was positively related to species richness of many taxa, both generalists (plants, butterflies, bees) and specialists (rare birds, woodland birds, plants, butterflies).
The results were used to create maps of HNV farmland. The proportion of semi-natural vegetation is a useful metric for identifying HNV type 1. HNV type 2 (defined as a mosaic of low-intensity habitats and structural elements) is more difficult to predict from surrogate variables, due to complex relationships between biodiversity and habitat heterogeneity and inadequacies of current remotely sensed data.
This approach, using fine-scaled field survey data collected at regular intervals, in conjunction with remotely sensed data offers potential for extrapolating modelled results nationally, and importantly, can be used to assess change over time
Effects of monosodium-L-glutamate administration on serum levels of reproductive hormones and cholesterol, epididymal sperm reserves and testicular histomorphology of male albino rats
This study investigated the effects of administration of monosodium L-glutamate (MSG) on serum gonadotrophin-releasing hormone (GnRH), luteinising hormone (LH), testosterone and total cholesterol (TC), cauda epididymal sperm reserves (CESR) and testicular histomorphology of adult male albino rats. Eighty-four rats, randomly assigned to 7 groups of 12 rats each, were used for the study. Varying low doses (0.25, 0.50 or 1.00 g/kg body weight) of MSG were administered orally or subcutaneously at 48-h intervals for six weeks. Serum GnRH, LH, testosterone and TC, and CESR were evaluated on days 14, 28 and 42 of MSG administration. Testicular histomorphology was evaluated on day 42. The results showed that the mean serum GnRH, LH and testosterone levels, and the CESR of all the treated groups were significantly (P < 0.05) lower than those of the untreated control on days 14, 28 and 42 of MSG administration. The mean serum TC levels of all the treated groups were also significantly (P < 0.05) lower than those of the control group on days 14 and 28. No lesions were observed on sections of the testes. It was concluded that MSG administration for 14, 28 and 42 days led to significantly lower serum levels of GnRH, LH, testosterone and TC, and significantly lower CESR
Assessing the cost of global biodiversity and conservation knowledge
Knowledge products comprise assessments of authoritative information supported by
standards, governance, quality control, data, tools, and capacity building mechanisms.
Considerable resources are dedicated to developing and maintaining knowledge
products for biodiversity conservation, and they are widely used to inform policy and
advise decision makers and practitioners. However, the financial cost of delivering this
information is largely undocumented. We evaluated the costs and funding sources for
developing and maintaining four global biodiversity and conservation knowledge
products: The IUCN Red List of Threatened Species, the IUCN Red List of
Ecosystems, Protected Planet, and the World Database of Key Biodiversity Areas.
These are secondary data sets, built on primary data collected by extensive networks
of expert contributors worldwide. We estimate that US116-204
million), plus 293 person-years of volunteer time (range: 278-308 person-years) valued
at US12-16 million), were invested in these four knowledge
products between 1979 and 2013. More than half of this financing was provided
through philanthropy, and nearly three-quarters was spent on personnel costs. The
estimated annual cost of maintaining data and platforms for three of these knowledge
products (excluding the IUCN Red List of Ecosystems for which annual costs were not
possible to estimate for 2013 ) is US6.2-6.7 million).
We estimated that an additional US12 million. These costs
are much lower than those to maintain many other, similarly important, global
knowledge products. Ensuring that biodiversity and conservation knowledge products
are sufficiently up to date, comprehensive and accurate is fundamental to inform
decision-making for biodiversity conservation and sustainable development. Thus, the
development and implementation of plans for sustainable long-term financing for them
is critical
Low-calcium diet prevents fructose-induced hyperinsulinemia and ameliorates the response to glucose load in rats
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