Effects of the topical hemostatic agent Ankaferd Blood Stopper on the incidence of alveolar osteitis after surgical removal of an impacted mandibular third molar

Background: Alveolar osteitis (AO) is a commonly seen post‑operative complication during the wound‑healing period after permanent tooth extraction or surgical removal of impacted third molar teeth.Objectives: The aim of this clinical study was to evaluate the effects of administration of the topical hemostatic agent Ankaferd Blood Stopper (ABS) into the socket on AO formation after impacted mandibular third molar extraction.Patients and Methods: Bilaterally, 100 half‑impacted mandibular third molars were extracted in 50 patients. Then, 1.0 mL ABS was administered to achieve hemostasis in one half of the sockets and as a control, the other half was irrigated with 1.0 mL physiological serum after surgery.Results: There was no statistically significant difference in terms of AO formation (P > 0.05) between the extraction sites. However, the postoperative pain in ABS administration sites was higher than in the other sites for the first 2 days after surgery (P < 0.05).Conclusions: The results showed that ABS administration did not increase the incidence of AO formation. Thus, ABS can be used safely for hemostasis after impacted mandibular third molar surgery.Key words: Alveolar osteitis, Ankaferd Blood Stopper, hemostasis, third mola

The Effect of GHR/exon-3 Polymorphism and Serum GH, IGF-1 and IGFBP-3 Levels in Diabetes and Coronary Heart Disease

Aim: The present study investigated the effects of growth hormone (GH), insulin-like growth factor-1 (IGF-1), insulin-like growth factor binding protein-3 (IGFBP-3) and GH-receptor (GHR)/exon-3 polymorphism on diabetes mellitus (DM) and coronary heart disease (CHD) patients. Patients and Methods: Ninety patients with CHD, 90 patients with DM and 96 controls were included in this study. The GH, IGF-1 and IGFBP-3 serum levels were measured with enzyme-linked immunosorbent assay. GHR/exon-3 variants were determined by multiplex-polymerase chain reaction. Results: The frequency of all alleles and genotypes in all study groups were distributed according to the Hardy-Weinberg equilibrium. In addition, any association between GHR/exon-3 variants and the presence of risk factors were detected. The blood levels of GH, IGF-1 and IGFBP-3 were not distributed according to GHR/exon-3 variants. However, in the DM group, higher levels of IGF-1 and lower levels of GH and IGFBP-3, and in CHD group lower levels of IGF-1, GH and IGFBP-3 were observed. The order of GH levels were DM<CHD< Controls; IGF-1 levels were CHD<Controls<DM and IGFBP-3 levels were CHD<DM<Controls. Conclusion: No direct effect of GHR/exon-3 polymorphism was observed in DM or CHD patients. However GH, IGF-1, IGFBP-3 and insulin were thought to act together to establish body homeostasis in patients with DM and CHD

Design and Myoelectric Control of an Active Orthosis Device Using Finite State Machine Algorithm

This paper presents a novel myoelectric controlled active hip-knee-ankle-foot orthosis (A-HKAFO) designed to assist lower limb disorders. The proposed orthosis device2 consists of a polypropylene shell and a metal hinge joint, is designed to help patients during gait rehabilitation after neurological injury, and assist people who have difficulty walking3 due to obesity, sports injuries. The system also can use4 for studying human gait biomechanics5. A myoelectric control law strategy is proposed using a finite state machine (FSM) method. The algorithm is activated by users' intend to forward or backward stepping6. The electromyogram (EMG) signals from lower limb7 and device motion data were8 used for the control of A-HKAFO. In order to determine the last location of the user after movement, physical feedback is utilized from the mechanical system

Dynamic assessment of Capparis spinosa buds on survival of periodontal ligament cells using a real‑time cell analysis method

Background: Tooth avulsion is the most severe type of traumatic dental injuries and it results in the complete displacement of the tooth out of its socket in alveolar bone. Reimplantation of the tooth is considered to be a best treatment modality due to its biological and psychological advantages. Its prognosis depends on the extra alveolar time, the storage medium, and the patient’s general health.Objective: The aim of this study was to evaluate the effect of Capparis spinosa (C. spinosa) in maintaining the viability of human periodontal ligament (PDL) cells using a real‑time cell analysis method.Materials and Methods: Periodontal ligament cells were obtained from healthy human third molars extracted for orthodontic purposes. The storage media tested were: Dulbecco’s Modified Eagle Medium (DMEM), C. spinosa, Hank’s Balanced Salt Solution (HBSS), and light milk. A real‑time cell analyzer system was used to evaluate cell viability. After seeding cell suspensions into the wells of the E‑plate 96, PDL cells were treated with each of tested media and monitored for every 5 min for 26 h. Statistical analysis of the data was accomplished using one‑way analysis of variance complemented by the Tukey test. The level of significance was set at P &lt; 0.05.Results: Dulbecco’s Modified Eagle Medium (control) and C. spinosa groups had significantly higher cell index values compared with the HBSS and light milk (P &lt; 0.05). Although, C. spinosa showed better results than DMEM (control), but this difference was not found statistically significant.Conclusion: Capparis spinosa can be a suitable, alternative storage medium for avulsed teeth.Key words: Avulsion, Capparis spinosa, cell viability, storage media, real time cell analyze