Pointless curves of genus three and four

A curve over a field k is pointless if it has no k-rational points. We show that there exist pointless genus-3 hyperelliptic curves over a finite field F_q if and only if q < 26, that there exist pointless smooth plane quartics over F_q if and only if either q < 24 or q = 29 or q = 32, and that there exist pointless genus-4 curves over F_q if and only if q < 50.Comment: LaTeX, 15 page

Helium, neon, and tritium in the Black Sea

Measurements of the 3He/4He ratio, and concentrations of helium, neon, and tritium have been made in samples collected at station 1355 in the Black Sea during the 1975 cruise of R/V Chain. Helium concentrations increase rapidly from 400 m to about 1000 m and then less rapidly below 1000 m. The maximum He concentration excess is about 16% above solubility equilibrium. Neon concentrations are constant, within a few percent of solubility equilibrium, below 400 m. The He-Ne data thus clearly indicate a large component of radiogenic 4He in the deep water from decay of U and Th in the bottom sediments. Tritium concentrations decrease from 67.2 T.U. at the surface to near-zero values at 726 m, 968 m, and 1358 m. Two deeper samples at 1745 m and 1939 m contain some tritium—0.6 ± 0.3 T.U. and 0.7 ± 0.2 T.U. respectively, which shows that a small amount of high-tritium surface water has descended to the bottom during the past twenty years. A one-dimensional three-box model using our tritium concentrations and the geothermal heat flux values measured by Erickson and Simmons (1974) gives vertical exchange times of 440 ± 180 years between the deep water (1000 m-2000 m) and the middle water (400 m-1000 m), and 125 ± 75 years between the middle water and a layer above from 200 m to 400 m in the salinity interval 21.50‰-22.00‰. The fluxes of radiogenic 4He and primordial 3He into the deep water are found to be 1.3 ± 0.5 × 105 atoms cm—2 sec—1 and 1.1 ± 0.6 atoms cm—2 sec—1 respectively which may be compared with world-ocean estimates of Craig et al. (1975)—3 ± 1.5 x 105 atoms cm—2 sec—1 and 4 ± 1 atoms cm—2 sec—1

Radiogenic helium in Baffin Bay bottom water

3He-4He ratios, dissolved He and Ne, and tritium have been measured in 87 seawater samples collected at 14 stations in Baffin Bay, Davis Strait, and in the sounds through which Arctic water flows into Baffin Bay…

Changes in active site histidine hydrogen bonding trigger cryptochrome activation

Cryptochrome (CRY) is the principal light sensor of the insect circadian clock. Photoreduction of the Drosophila CRY (dCRY) flavin cofactor to the anionic semiquinone (ASQ) restructures a C-terminal tail helix (CTT) that otherwise inhibits interactions with targets that include the clock protein Timeless (TIM). All-atom molecular dynamics (MD) simulations indicate that flavin reduction destabilizes the CTT, which undergoes large-scale conformational changes (the CTT release) on short (25 ns) timescales. The CTT release correlates with the conformation and protonation state of conserved His378, which resides between the CTT and the flavin cofactor. Poisson-Boltzmann calculations indicate that flavin reduction substantially increases the His378 pKa. Consistent with coupling between ASQ formation and His378 protonation, dCRY displays reduced photoreduction rates with increasing pH; however, His378Asn/Arg variants show no such pH dependence. Replica-exchange MD simulations also support CTT release mediated by changes in His378 hydrogen bonding and verify other responsive regions of the protein previously identified by proteolytic sensitivity assays. His378 dCRY variants show varying abilities to light-activate TIM and undergo self-degradation in cellular assays. Surprisingly, His378Arg/Lys variants do not degrade in light despite maintaining reactivity toward TIM, thereby implicating different conformational responses in these two functions. Thus, the dCRY photosensory mechanism involves flavin photoreduction coupled to protonation of His378, whose perturbed hydrogen-bonding pattern alters the CTT and surrounding regions

PCR-based detection of mobile genetic elements in total community DNA

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Dynamics of ampicillin-resistant Enterococcus faecium clones colonizing hospitalized patients: data from a prospective observational study

<p>Abstract</p> <p>Background</p> <p>Little is known about the dynamics of colonizing <it>Enterococcus faecium </it>clones during hospitalization, invasive infection and after discharge.</p> <p>Methods</p> <p>In a prospective observational study we compared intestinal <it>E. faecium </it>colonization in three patient cohorts: 1) Patients from the Hematology Unit at the University Hospital Basel (UHBS), Switzerland, were investigated by weekly rectal swabs (RS) during hospitalization (group 1a, n = 33) and monthly after discharge (group 1b, n = 21). 2) Patients from the Intensive Care Unit (ICU) at the University Medical Center Utrecht, the Netherlands (group 2, n = 25) were swabbed weekly. 3) Patients with invasive <it>E. faecium </it>infection at UHBS were swabbed at the time of infection (group 3, n = 22). From each RS five colonies with typical <it>E</it>. <it>faecium </it>morphology were picked. Species identification was confirmed by PCR and ampicillin-resistant <it>E. faecium </it>(ARE) isolates were typed using Multiple Locus Variable Number Tandem Repeat Analysis (MLVA). The Simpson's Index of Diversity (SID) was calculated.</p> <p>Results</p> <p>Out of 558 ARE isolates from 354 RS, MT159 was the most prevalent clone (54%, 100%, 52% and 83% of ARE in groups 1a, 1b, 2 and 3, respectively). Among hematological inpatients 13 (40%) had ARE. During hospitalization, the SID of MLVA-typed ARE decreased from 0.745 [95%CI 0.657-0.833] in week 1 to 0.513 [95%CI 0.388-0.637] in week 3. After discharge the only detected ARE was MT159 in 3 patients. In the ICU (group 2) almost all patients (84%) were colonized with ARE. The SID increased significantly from 0.373 [95%CI 0.175-0.572] at week 1 to a maximum of 0.808 [95%CI 0.768-0.849] at week 3 due to acquisition of multiple ARE clones. All 16 patients with invasive ARE were colonized with the same MLVA clone (<it>p </it>< 0.001).</p> <p>Conclusions</p> <p>In hospitalized high-risk patients MT159 is the most frequent colonizer and cause of invasive <it>E. faecium </it>infections. During hospitalization, ASE are quickly replaced by ARE. Diversity of ARE increases on units with possible cross-transmission such as ICUs. After hospitalization ARE are lost with the exception of MT159. In invasive infections, the invasive clone is the predominant gut colonizer.</p

Health-related quality of life in early onset scoliosis patients treated with the spring distraction system:what to expect in the first 2 years after surgery

Purpose: The Spring Distraction System (SDS) is a novel “growth-friendly” implant for the treatment of Early-Onset Scoliosis (EOS). This prospective study aims to determine the evolution of the “24-Item Early-Onset Scoliosis Questionnaire” (EOSQ-24) scores during 2-year follow-up after SDS surgery. Secondary aims include investigating the relation between EOSQ-24 scores and EOS etiology, and evaluating the impact of an unplanned return to the operating room (UPROR) on HRQoL. Methods: All SDS patients with at least 2-year follow-up were included. Caregivers completed the EOSQ-24 pre-operatively, post-operatively, and at 6, 12, and 24 month follow-up. Mean total and -domain scores were graphed over time. Repeated-measures ANOVA analyzed the influence of etiology on EOSQ-24 scores. Multiple regression analyzed associations between UPRORs and EOSQ-24 scores. Results: Forty-nine patients were included. Mean total EOSQ-24 scores decreased from 70 pre-operatively to 66 post-operatively, then gradually increased to 75 (24 months). Most domains exhibited changes over time, with initial declines, but eventually surpassing pre-operative levels after 2-year follow-up. Neuromuscular/Syndromic patients had lower scores, but showed similar improvements over time compared with other etiologies. Multiple regression showed lower Parental Burden domain score (− 14 points) in patients with UPRORs, although no significant reductions were found in total score, or in other domains. Conclusion: HRQoL decreases immediately following SDS surgery but quickly recovers and exceeds pre-operative levels at 2-year follow-up in all domains. Neuromuscular/Syndromic patients have lower initial scores, but progress similarly over time. UPRORs do not influence EOSQ-24 scores, except for a negative impact on the Parental Burden domain in the short term. Level of Evidence: III.</p