Biomass production of site selective 13C/15N nucleotides using wild type and a transketolase E. coli mutant for labeling RNA for high resolution NMR

Characterization of the structure and dynamics of nucleic acids by NMR benefits significantly from position specifically labeled nucleotides. Here an E. coli strain deficient in the transketolase gene (tktA) and grown on glucose that is labeled at different carbon sites is shown to facilitate cost-effective and large scale production of useful nucleotides. These nucleotides are site specifically labeled in C1′ and C5′ with minimal scrambling within the ribose ring. To demonstrate the utility of this labeling approach, the new site-specific labeled and the uniformly labeled nucleotides were used to synthesize a 36-nt RNA containing the catalytically essential domain 5 (D5) of the brown algae group II intron self-splicing ribozyme. The D5 RNA was used in binding and relaxation studies probed by NMR spectroscopy. Key nucleotides in the D5 RNA that are implicated in binding Mg2+ ions are well resolved. As a result, spectra obtained using selectively labeled nucleotides have higher signal-to-noise ratio compared to those obtained using uniformly labeled nucleotides. Thus, compared to the uniformly 13C/15N-labeled nucleotides, these specifically labeled nucleotides eliminate the extensive 13C–13C coupling within the nitrogenous base and ribose ring, give rise to less crowded and more resolved NMR spectra, and accurate relaxation rates without the need for constant-time or band-selective decoupled NMR experiments. These position selective labeled nucleotides should, therefore, find wide use in NMR analysis of biologically interesting RNA molecules

UMR 9018 - Equipe 2 - « Interactions hôte-tumeur dans les carcinomes ORL»

International audiencePhilippe Gorphe, chirurgie ORL Caroline Even, oncologie médicale Cliniciens rattachés à l'équipe General background Public health problem Exposure to carcinogens (tobacco, alcohol, …) Possible etiologic contributions of viruses e.g. Human PapillomaViru

UMR 9018 - Equipe 2 - « Interactions hôte-tumeur dans les carcinomes ORL»

International audiencePhilippe Gorphe, chirurgie ORL Caroline Even, oncologie médicale Cliniciens rattachés à l'équipe General background Public health problem Exposure to carcinogens (tobacco, alcohol, …) Possible etiologic contributions of viruses e.g. Human PapillomaViru

Synthesis of graphene oxide/magnesium oxide nanocomposites with high-rate adsorption of methylene blue

A series of graphene oxide/magnesium oxide nanocomposites (GO/MgO NCs) were and applied for the removal of Methylene Blue (MB) from aqueous solutions. The prepared NCs were characterized using scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectrum, X-ray photoelectron spectroscopy, and thermogravimetric analysis. The results showed that MgO particles was successfully decorated on GO. The impacts of different experimental variables on the removal of MB including GO/MgO NCs dosage, pH, contact time, and initial MB concentration were investigated. The experimental analysis of adsorption isotherms indicated that adsorption data was best fit with the Langmuir isotherm model. Among the three different synthesized weight ratios of GO/MgO (5:1, 1:1, and 1:5), 5:1 ratio showed the maximum adsorption capacity as 833 mg/g, which is higher than any previously reported GO-based composites. The synthesized GO/MgO NC is also observed to have higher adsorption capacity for MB removal, in comparison with pure GO and MgO. The kinetic adsorption data was best described by pseudo-second-order kinetic model. The pH of point of zero charge (pH(pzc)) of GO/MgO NCs was determined to be 9.7, 10.5, and 10.5 for ratios 5:1, 1:1, and 1:5, respectively. The results revealed that electrostatic attraction can be the dominant mechanism of adsorption between GO/MgO NCs and MB for pH above pH(pzc); whereas for pH below pH(pzc), other adsorption mechanisms such as hydrogen bonding and pi-pi interaction may attribute to adsorption. The high adsorption capacity of GO/MgO composites, thus makes it a promising adsorbent for water and wastewater treatment. (C) 2016 Elsevier B.V. All rights reserved