Getting Out of the Laboratory to Make Experiments Real: Can Sports Fans Influence Muay Thai Judges?

To find out if one thing actually causes another, carefully controlled experiments are needed. Experiments usually take place in a laboratory. However, to examine how people respond to things that happen in real life, in particular places at particular times, it can also be important to step outside the laboratory. This article discusses how to have enough control in an experiment to be confident that something caused something else to happen, yet to also be confident the same effect would happen with other people, at other times, and in other places. This article will explain why it can be important to conduct experiments in real-life settings and will illustrate this using an experiment that a colleague and I conducted on the effect of crowd noise on the judges during a sport called Muay Thai. However, it will begin by exploring how experiments are used to find cause and effect

‘We have to wait in a queue for our turn quite a bit’ Examining children’s physical activity during primary physical education lessons

The overall purpose of this study was to examine children’s physical activity (PA) during primary physical education (PE). This was achieved through the following two research objectives: (1) to measure children’s PA, lesson context and teacher promotion of PA during PE lessons; and (2) to explore teachers’ and children’s perspectives on PA levels during PE lessons. Evidence suggests that children’s PA during PE is below recommended levels and further research is required to understand the reasons why. Through a mixed method design, 138 children were observed using the System for Observing Fitness and Instruction Time, 80 children participated in group interviews, and 13 teachers were interviewed, across three primary schools in England. Findings indicated that the mean percentage of lesson time allocated to moderate to vigorous PA (MVPA) was 42.4% and the average lesson length was 35.3 minutes. Qualitative themes identified were: ‘knowledge and beliefs’; ‘teacher pedagogy’; and ‘teacher development’. The findings indicate that a change in perspective is needed, which includes a focus on PA during primary PE lessons. Intervention work is required that targets teachers’ knowledge and beliefs towards PE along with the development of effective teaching strategies. However, this needs to be grounded in an ecological approach which will allow researchers and schools to target the various levels of influence. It is strongly recommended that interventions are grounded in behaviour change theory, as this study indicates that sharing knowledge about pedagogical strategies to increase children’s MVPA does not necessarily produce changes in teachers’ behaviours

Barriers to Physical Activity: A Comparison of Afghans Living in the UK and Afghanistan

It is important to understand barriers to Physical Activity (PA), particularly for under researched populations. While a lack of PA has been acknowledged as an important issue for those living in Afghanistan, there have been no published studies exploring barriers to PA within this population. Therefore, the purpose of this study was to explore barriers to PA for Afghans and how these differ depending on where they reside: UK or Afghanistan. 930 participants, 578 (291 males; 287 females) were recruited from Afghanistan and 352 (267 males; 85 females) from the UK. A culturally nuanced questionnaire exploring barriers to PA was developed and used to collect data via an online survey software programme. Bayesian ordinal models explored differences between population groups, with results suggesting some barriers are shared across populations but others more pertinent for a particular group. Lack of time and being too tired were barriers for both populations, but were rated higher by those living in Afghanistan; as was a lack of confidence and being uncomfortable with exercise clothing. Afghan females perceived a lack of single- sex facilities, not being able to participate in PA with males, and having to be fully covered outside of the home as important barriers to their PA, but these were more of a barrier for those residing in the UK. The findings highligh

The Relationship between Training Load Measures and Next-Day Well-Being in Rugby Union Players

The aim of this study is to identify the relationship between different internal and external load measures and next day subjective wellbeing. With institutional ethics approval, ten academy rugby union players (Five forwards, and five backs) with a local National League One club agreed to participate in the study (aged; 18.4 ± 1.0 years, height; 181.3 ± 5.9 cm, body mass 85.9 ± 13.0 kg, VO2max 56.2 ± 6.8 mL·kg−1·min−1). Before the 6-week in-season data collection period, participants completed an incremental treadmill test to determine lactate thresholds at 2 mmol·L−1 (LT) and 4 mmol·L−1 and the heart rate blood lactate (HR-BLa) profile for individualized training impulse (iTRIMP) calculations. Internal training load was quantified using Banister’s TRIMP, Edward’s TRIMP, Lucia’s TRIMP, individualised TRIMP and session-RPE. External training load was reported using total distance, PlayerLoadTM, high-speed distances (HSD) > 18 km∙h−1 and >15 km∙h−1, and individualized high-speed distance (iHSD) based on each player’s velocity at OBLA. On arrival and prior to all training sessions players completed a well-being questionnaire (WB). Bayesian linear mixed model analysis identified that a range of internal and external load measures explained between 30% and 37% of next-day total wellbeing and between 65% and 67% of next-day perceived stress. All other internal and external load measures demonstrated very weak to moderate relationships (R2 = 0.08 to 0.39) with all other wellbeing components. Internal sRPE, iTRIMP and bTRIMP loads alongside external HSD loads provide coaches with the most practical measures to influence players’ perceived wellbeing

The ongoing effects of the COVID-19 pandemic on perceived physical activity, physical function and mood of older adults in the U.K:A follow-up study (March 2020-June 2021)

Coronavirus (COVID-19) and its variants, continue to spread globally more than two years after the discovery of the wild-type virus in Wuhan, China. Following the onset of COVID-19, fluctuating restrictions have likely impacted the daily lives of older adults living in the United Kingdom (UK). Subsequently, the longer term effects of COVID-19 on physical activity levels, perceived physical function and mood of older adults are unclear. Therefore, the present study aimed to follow a group of older adult's living in the UK for one year, to monitor physical activity levels, perceived physical function and mood. A longitudinal, mixed-methods, observational study was conducted using self-administered, online surveys at 3-month intervals between March 2020 and June 2021. A total of 100 participants (46 males [age: 76 ± 5 years] and 54 females [age:74 ± 4 years]) completed all surveys. Bayesian analysis allowed calculation of direct probabilities whilst incorporating our prior knowledge. Throughout this period, older adults maintained or increased their pre-lockdown physical activity levels despite a decrease in intensity of effort of physical activity tasks, whilst sitting time increased at two of the follow-up time-points. Furthermore, perceived physical function decreased (ps = 91.78%;>1.21 AU) and mood undu�lated in a pattern that reflected the tightening and easing of restrictions. Despite total physical activity being maintained, perceived physical function decreased by a small but clinically meaningful margin

Genomic, Pathway Network, and Immunologic Features Distinguishing Squamous Carcinomas

This integrated, multiplatform PanCancer Atlas study co-mapped and identified distinguishing molecular features of squamous cell carcinomas (SCCs) from five sites associated with smokin

Pan-Cancer Analysis of lncRNA Regulation Supports Their Targeting of Cancer Genes in Each Tumor Context

Long noncoding RNAs (lncRNAs) are commonly dys-regulated in tumors, but only a handful are known toplay pathophysiological roles in cancer. We inferredlncRNAs that dysregulate cancer pathways, onco-genes, and tumor suppressors (cancer genes) bymodeling their effects on the activity of transcriptionfactors, RNA-binding proteins, and microRNAs in5,185 TCGA tumors and 1,019 ENCODE assays.Our predictions included hundreds of candidateonco- and tumor-suppressor lncRNAs (cancerlncRNAs) whose somatic alterations account for thedysregulation of dozens of cancer genes and path-ways in each of 14 tumor contexts. To demonstrateproof of concept, we showed that perturbations tar-geting OIP5-AS1 (an inferred tumor suppressor) andTUG1 and WT1-AS (inferred onco-lncRNAs) dysre-gulated cancer genes and altered proliferation ofbreast and gynecologic cancer cells. Our analysis in-dicates that, although most lncRNAs are dysregu-lated in a tumor-specific manner, some, includingOIP5-AS1, TUG1, NEAT1, MEG3, and TSIX, synergis-tically dysregulate cancer pathways in multiple tumorcontexts

Spatial Organization and Molecular Correlation of Tumor-Infiltrating Lymphocytes Using Deep Learning on Pathology Images

Beyond sample curation and basic pathologic characterization, the digitized H&E-stained images of TCGA samples remain underutilized. To highlight this resource, we present mappings of tumorinfiltrating lymphocytes (TILs) based on H&E images from 13 TCGA tumor types. These TIL maps are derived through computational staining using a convolutional neural network trained to classify patches of images. Affinity propagation revealed local spatial structure in TIL patterns and correlation with overall survival. TIL map structural patterns were grouped using standard histopathological parameters. These patterns are enriched in particular T cell subpopulations derived from molecular measures. TIL densities and spatial structure were differentially enriched among tumor types, immune subtypes, and tumor molecular subtypes, implying that spatial infiltrate state could reflect particular tumor cell aberration states. Obtaining spatial lymphocytic patterns linked to the rich genomic characterization of TCGA samples demonstrates one use for the TCGA image archives with insights into the tumor-immune microenvironment

Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN