RLR-mediated antiviral innate immunity requires oxidative phosphorylation activity

Mitochondria act as a platform for antiviral innate immunity, and the immune system depends on activation of the retinoic acid-inducible gene I (RIG-I)-like receptors (RLR) signaling pathway via an adaptor molecule, mitochondrial antiviral signaling. We report that RLR-mediated antiviral innate immunity requires oxidative phosphorylation (OXPHOS) activity, a prominent physiologic function of mitochondria. Cells lacking mitochondrial DNA or mutant cells with respiratory defects exhibited severely impaired virus-induced induction of interferons and proinflammatory cytokines. Recovery of the OXPHOS activity in these mutants, however, re-established RLR-mediated signal transduction. Using in vivo approaches, we found that mice with OXPHOS defects were highly susceptible to viral infection and exhibited significant lung inflammation. Studies to elucidate the molecular mechanism of OXPHOS-coupled immune activity revealed that optic atrophy 1, a mediator of mitochondrial fusion, contributes to regulate the antiviral immune response. Our findings provide evidence for functional coordination between RLR-mediated antiviral innate immunity and the mitochondrial energy-generating system in mammals

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

A portable radioactive plume monitor using a silicon photodiode

In this study, a portable radioactive plume monitor using a silicon photodiode was developed for the detection of a radioactive plume (e.g. ^I, ^Cs and ^Cs) in an emergency situation. It was found that the background count rate was proportional to ambient dose equivalent rate and the detection limit for the monitor at 20 μSv h^ as an ambient dose equivalent rate was evaluated to be 187 Bq m^ using the ISO11929 method. These results suggest that the detection limit for the system can be decreased effectively by lead shielding with optimized thickness

RLR-mediated antiviral innate immunity requires oxidative phosphorylation activity

Mitochondria act as a platform for antiviral innate immunity, and the immune system depends on activation of the retinoic acid-inducible gene I (RIG-I)-like receptors (RLR) signaling pathway via an adaptor molecule, mitochondrial antiviral signaling. We report that RLR-mediated antiviral innate immunity requires oxidative phosphorylation (OXPHOS) activity, a prominent physiologic function of mitochondria. Cells lacking mitochondrial DNA or mutant cells with respiratory defects exhibited severely impaired virus-induced induction of interferons and proinflammatory cytokines. Recovery of the OXPHOS activity in these mutants, however, re-established RLR-mediated signal transduction. Using in vivo approaches, we found that mice with OXPHOS defects were highly susceptible to viral infection and exhibited significant lung inflammation. Studies to elucidate the molecular mechanism of OXPHOS-coupled immune activity revealed that optic atrophy 1, a mediator of mitochondrial fusion, contributes to regulate the antiviral immune response. Our findings provide evidence for functional coordination between RLR-mediated antiviral innate immunity and the mitochondrial energy-generating system in mammals

PRELIMINARY TECHNICAL DISCUSSION ON A NEW RADON AND ITS PROGENY CONTINUOUS MONITOR USING TWO-FILTER METHOD

Focusing on the scalability of Two-Filter Method, we started to develop a monitor for the concentration of radon and its progeny. In this study, we investigated the influence of a high-humidity environment on measuring radon concentration, and the influence of the decay chamber of the monitor on the measurement. In the high-humidity test, the conversion factor of (Bqm−3)/(cpm) tends to raise with increasing humidity. On the measurement of radon progeny, existence of the decay chamber of the monitor makes measurement sensitivity lower under environments of little aerosols. Radon concentration measurement by the developed monitor could be influenced by environmental humidity, and that counting loss could occur due to deposition of radon progeny inside of the decay chamber. Correction relating these would be needed based on the data of calibration tests

A portable radioactive plume monitor using a silicon photodiode

In this study, a portable radioactive plume monitor using a silicon photodiode was developed for the detection of a radioactive plume (e.g.131I,134Cs and137Cs) in an emergency situation. It was found that the background count rate was proportional to ambient dose equivalent rate and the detection limit for the monitor at 20 Sv h−1 as an ambient dose equivalent rate was evaluated to be 187 Bq m−3 using the ISO11929 method. These results suggest that the detection limit for the system can be decreased effectively by lead shielding with optimized thickness

Relationship between attributes of individual workers and concentration at work

Working environments are becoming far more diverse. For example, Activity-Based Working (ABW), a new style of working where workers choose their own work environment, is now attracting attention. An office that introduces ABW is comprised of various spaces, including open plan workspaces, corresponding to the ways of working. However, such an office may result in distraction. Therefore, it is necessary to identify the environmental factors that affect individual workers’ concentration at work. This study aimed to ascertain the relationship between individual workers’ attributes, such as the Big Five personality traits, personal control, and age, and the factors that distract them from their work. A questionnaire-based field survey was conducted at an office that partially adopted ABW to investigate the relationship between selection of seats and concentration at work. The results revealed different tendencies among workers for the type of work environment needed to concentrate according to their individual attributes. Additionally, it was suggested that the changing of seats lead to improved concentration at work, thus increasing productivity