Radon in Workplaces the Urgent Need of New Measurements and Devices

The existing passive radon monitors, their relative calibration facilities together with the past intercomparison exercises have been mission-oriented towards radon measurements in dwellings. These monitors have been successfully applied throughout the world for radon measurements in homes, characterized by temperatures in the range from 20 to 25°C and a relative humidity less than 50 R.H. A multitude of different problems may arise when these passive monitors are used in environment other than homes, such as in soil and in workplaces, where large humidity up to 100 RH and temperatures anywhere from 0°C to 40°C may be encountered. Under severe environmental conditions, different measurement errors may occur which have remained concealed to date. These errors may be caused by a drastic change of both the radon diffusivity through the and for the monitor housing respectively. permeation membranes or the radon absorption by the plastics, used for the track detector. For the compliance to the assessment of the occupational exposures, it is necessary to eliminate all the possible sources of errors, which may be conducive to litigation. Another important shortcoming of the existing passive monitors is the difficult to turn them on/off daily, as required for radon measurements in workplaces. Finally, most of the problems, listed above, can be solved by the exploitation of a new generation of passive monitors, known as Rn film-badges. These monitors are similar and often identical to neutron film-badges, which have proved to be very successful throughout the world for the personnel neutron dosimetry. In particular, the present paper will describe the unique characteristics of these radon film badges, such as compactness, fast time response, any desired response sensitivity, simplicity in turning them on and off, etc

Analysis and modelling of broad-band ferrite-based coaxial transmission-line transformers

The work presented in the paper focuses on accuracy of models for broad-band ferrite based coaxial transmission-line transformers. Soft-ferrites are largely used in VHF/UHF components allowing band enlargement on the low-edge side. Degradation of frequency performance on the high-edge side are produced both by ferrite losses, and by parasitic capacitance due to connection to the thermal and electrical ground in high power applications. Both a circuital model for low-power applications and a scalable e.m. model for high-power applications are presented and discussed. © 2010 EuMA

Neutron spectrometry at various altitudes in atmosphere by passive detector technique

A new experimental system, constituted by passive detectors, has been developed to measure neutron spectra at various altitudes in the atmosphere. The knowledge of the neutron spectrum is required to evaluate with a good accuracy the neutron contribution to the total dose, due to the cosmic ray exposure, in fact the flux-to-dose conversion factors strongly depend on neutron energy. Moreover, in many dosimetric applications, as the dose evaluation to the aircrew in service on intercontinental flights, the passive system is not only the most convenient but it is often the unique technique. The experimental system is constituted by the passive bubble detector BD100R, polycarbonate foils, polycarbonate bottles, sensitive in low and intermediate neutron energy range, and the bismuth stack, sensitive in the high energy range. Experimental data were obtained in high mountain measurements at Matterhorn (3600 m altitude, 46 N ) and Chacaltaya (5230 m altitude, 16 S), during flights at 12000 m and on board of stratospheric balloons at 38000 m. All the spectra obtained show, as expected, the evaporation peak around 1 MeV and the second direct bump around 100 MeV; the results, different in the neutron flux intensity, confirm the satisfactory sensitivity of this experimental technique

Epstein-Barr virus nuclear antigen 3A protein regulates CDKN2B transcription via interaction with MIZ-1

The Epstein-Barr virus (EBV) nuclear antigen 3 family of protein is critical for the EBV-induced primary B-cell growth transformation process. Using a yeast two-hybrid screen we identified 22 novel cellular partners of the EBNA3s. Most importantly, among the newly identified partners, five are known to play direct and important roles in transcriptional regulation. Of these, the Myc-interacting zinc finger protein-1 (MIZ-1) is a transcription factor initially characterized as a binding partner of MYC. MIZ-1 activates the transcription of a number of target genes including the cell cycle inhibitor CDKN2B. Focusing on the EBNA3A/MIZ-1 interaction we demonstrate that binding occurs in EBV-infected cells expressing both proteins at endogenous physiological levels and that in the presence of EBNA3A, a significant fraction of MIZ-1 translocates from the cytoplasm to the nucleus. Moreover, we show that a trimeric complex composed of a MIZ-1 recognition DNA element, MIZ-1 and EBNA3A can be formed, and that interaction of MIZ-1 with nucleophosmin (NPM), one of its coactivator, is prevented by EBNA3A. Finally, we show that, in the presence of EBNA3A, expression of the MIZ-1 target gene, CDKN2B, is downregulated and repressive H3K27 marks are established on its promoter region suggesting that EBNA3A directly counteracts the growth inhibitory action of MIZ-1

Mmf1p, a novel yeast mitochondrial protein conserved throughout evolution and involved in maintenance of the mitochondrial genome

A novel protein family (p14.5, or YERO57c/YJGFc) highly conserved throughout evolution has recently been identified. The biological role of these proteins is not yet well characterized. Two members of the p14.5 family are present in the yeast Saccharomyces cerevisiae. In this study, we have characterized some of the biological functions of the two yeast proteins. Mmf1p is a mitochondrial matrix factor, and homologous Mmf1p factor (Hmf1p) copurifies with the soluble cytoplasmic fraction. Δmmf1 cells lose mitochondrial DNA (mtDNA) and have a decreased growth rate, while Δhmf1 cells do not display any visible phenotype. Furthermore, we demonstrate by genetic analysis that Mmf1p does not play a direct role in replication and segregation of the mtDNA. rho(+) Δmmf1 haploid cells can be obtained when tetrads are directly dissected on medium containing a nonfermentable carbon source. Our data also indicate that Mmf1p and Hmf1p have similar biological functions in different subcellular compartments. Hmf1p, when fused with the Mmf1p leader peptide, is transported into mitochondria and is able to functionally replace Mmf1p. Moreover, we show that homologous mammalian proteins are functionally related to Mmf1p. Human p14.5 localizes in yeast mitochondria and rescues the Δmmf1-associated phenotypes. In addition, fractionation of rat liver mitochondria showed that rat p14.5, like Mmf1p, is a soluble protein of the matrix. Our study identifies a biological function for Mmf1p and furthermore indicates that this function is conserved between members of the p14.5 family

Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation

Epidermal keratinocytes are used as a cell source for autologous and allogenic cell transplant therapy for skin burns. The question addressed here is to determine whether the culture process may induce cellular, molecular, or genetic alterations that might increase the risk of cellular transformation. Keratinocytes from four different human donors were investigated for molecular and cellular parameters indicative of transformation status, including (i) karyotype, (ii) telomere length, (iii) proliferation rate, (iv) epithelial-mesenchymal transition, (v) anchorage-independent growth potential, and (vi) tumorigenicity in nude mice. Results show that, despite increased cell survival in one keratinocyte strain, none of the cultures displayed characteristics of cell transformations, implying that the culture protocol does not generate artefacts leading to the selection of transformed cells. We conclude that the current protocol does not result in an increased risk of tumorigenicity of transplanted cells

Prognostic significance of non-HPV16 genotypes in oropharyngeal squamous cell carcinoma

Recent studies have found that cases with oropharyngeal squamous cell carcinoma (OPSCC) positive for HPV16 genotype have better overall survival compared with cases positive for other HPV genotypes. We sought to further replicate these studies and determine if this relationship is modified by expression of p16 tumor suppressor protein

A compact Time-Of-Flight detector for space applications: The LIDAL system

Abstract LIDAL (Light Ion Detector for ALTEA system) is a compact detector designed to upgrade ALTEA (Anomalous Long Term Effects on Astronauts) silicon detector apparatus, in order to study in detail the low-Z part of ions spectrum inside the International Space Station (ISS) and to enhance the Particle Identification (PID) capability of the system. The new detector is designed to trigger ALTEA and to perform Time-Of-Flight measurements. It is based on plastic scintillators for fast timing applications read by Photo-Multiplier-Tubes (PMTs). A custom Front End Electronics (FEE) has been designed to reach time resolutions less than 100 ps ( σ ) for protons. A LIDAL prototype has been developed at the University of Rome Tor Vergata to test the timing performance of the scintillators, the PMTs and of the custom FEE using the proton beam line at the TIFPA (Trento Institute for Fundamentals Physics Applications) center in Trento, Italy. The results of these tests are reported and discussed. They have also been used for a preliminary evaluation of the Particle Identification (PID) capability of the final LIDAL-ALTEA detector system in response to the ions spectra expected on-board the ISS

Learning object relationships which determine the outcome of actions

Peer reviewedPublisher PD