Estilos de aprendizagem dos estudantes da disciplina de endodontia de uma universidade privada no sul do Brasil

The learning styles of Endodontics students from an undergraduate Dentistry course were evaluated using the Felder-Soloman Learning Styles Index. 144 students participated, from the morning and night shifts, between May and June 2016. Of these, 80.6% were female and 68.5% of the morning shift. The average age was 22.94 years and the median grade in the discipline was 6.52. The predominant styles were sensory, visual, active and sequential, respectively. The correlation between age and shift, and the intensity of each learning style had no statistically significant difference (p ˃ 0.05). Women were more sensory and men more visual (p = 0.010). In the correlation between the annual average and the styles, there was a significant difference for the sensory and the intuitive (p <0.001). Most had a mixed preference between learning styles, regardless of the shift.Los estilos de aprendizaje de los estudiantes de Endodoncia de un curso de pregrado en Odontología se evaluaron utilizando el índice de estilos de aprendizaje de Felder-Soloman. Participaron 144 estudiantes, de los turnos matutino y nocturno, entre mayo y junio de 2016. De estos, el 80,6% fueron mujeres y el 68,5% del turno matutino. La edad promedio fue de 22,94 años y la nota media en la disciplina fue de 6,52. Los estilos predominantes fueron sensorial, visual, activo y secuencial, respectivamente. La correlación entre la edad y el turno, y la intensidad de cada estilo de aprendizaje no tuvo diferencias estadísticamente significativas (p ˃ 0.05). Las mujeres eran más sensoriales y los hombres más visuales (p = 0,010). En la correlación entre el promedio anual y los estilos, hubo diferencia significativa para el sensorial y el intuitivo (p <0,001). La mayoría tenía una preferencia mixta entre los estilos de aprendizaje, independientemente del cambio.Foram avaliados os estilos de aprendizagem dos estudantes de Endodontia de um curso de graduação em Odontologia, por meio do Índice de Estilos de Aprendizagem de Felder-Soloman. Participaram 144 estudantes, dos turnos matutino e noturno, entre maio e junho de 2016. Destes, 80,6% eram do gênero feminino e 68,5% do turno da manhã. A média de idade foi de 22,94 anos e a mediana de notas na disciplina foi de 6,52. Os estilos predominantes foram sensorial, visual, ativo e sequencial, respectivamente. A correlação entre idade e turno, e a intensidade de cada estilo de aprendizado não tiveram diferença estatisticamente significativa (p ˃ 0,05). As mulheres foram mais sensoriais e os homens mais visuais (p = 0,010). Na correlação entre média anual e estilos, houve diferença significativa para o sensorial e o intuitivo (p < 0,001). A maioria teve preferência mista entre os estilos de aprendizagem, independentemente do turno

Influence of antimicrobial solutions in the decontamination and adhesion of glass-fiber posts to root canals

AbstractObjective This study evaluated the effect of root canal disinfectants on the elimination of bacteria from the root canals, as well as their effect on glass-fiber posts bond strength.Material and Methods Fifty-three endodontically treated root canals had post spaces of 11 mm in length prepared and contaminated with E. faecalis. For CFU/ml analysis, eight teeth were contaminated for 1 h or 30 days (n=4). Teeth were decontaminated with 5% NaOCl, 2% CHX, or distilled water. As control, no decontamination was conducted. After decontamination, sterile paper points were used to collect samples, and CFU/ml were counted. For push-out, three groups were evaluated (n=15): irrigation with 2.5% NaOCl, 2% CHX, or sterile distilled water. A bonding agent was applied to root canal dentin, and a glass-fiber post was cemented with a dual-cured cement. After 24 h, 1-mm-thick slices of the middle portion of root canals were obtained and submitted to the push-out evaluation. Three specimens of each group were evaluated in scanning electron microscopy (SEM). Data were analyzed with one-way ANOVA and Dunnett’s T3 test (α=0.05).Results The number of CFU/ml increased from 1 h to 30 days of contamination in control and sterile distilled water groups. Decontamination with NaOCl was effective only when teeth were contaminated for 1 h. CHX was effective at both contamination times. NaOCl did not influence the bond strength (p>0.05). Higher values were observed with CHX (p<0.05). SEM showed formation of resin tags in all groups.Conclusion CHX showed better results for the irrigation of contaminated root canals both in reducing the bacterial contamination and in improving the glass-fiber post bonding

Influence of antimicrobial solutions in the decontamination and adhesion of glass-fiber posts to root canals

AbstractObjective This study evaluated the effect of root canal disinfectants on the elimination of bacteria from the root canals, as well as their effect on glass-fiber posts bond strength.Material and Methods Fifty-three endodontically treated root canals had post spaces of 11 mm in length prepared and contaminated with E. faecalis. For CFU/ml analysis, eight teeth were contaminated for 1 h or 30 days (n=4). Teeth were decontaminated with 5% NaOCl, 2% CHX, or distilled water. As control, no decontamination was conducted. After decontamination, sterile paper points were used to collect samples, and CFU/ml were counted. For push-out, three groups were evaluated (n=15): irrigation with 2.5% NaOCl, 2% CHX, or sterile distilled water. A bonding agent was applied to root canal dentin, and a glass-fiber post was cemented with a dual-cured cement. After 24 h, 1-mm-thick slices of the middle portion of root canals were obtained and submitted to the push-out evaluation. Three specimens of each group were evaluated in scanning electron microscopy (SEM). Data were analyzed with one-way ANOVA and Dunnett’s T3 test (α=0.05).Results The number of CFU/ml increased from 1 h to 30 days of contamination in control and sterile distilled water groups. Decontamination with NaOCl was effective only when teeth were contaminated for 1 h. CHX was effective at both contamination times. NaOCl did not influence the bond strength (p>;0.05). Higher values were observed with CHX (

Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis Distribuição de biótipos e atividade leucotóxica de Aggregatibacter actinomycetemcomitans isolados de pacientes brasileiros com periodontite crônica

Aggregatibacter actinomycetemcomitans is an important etiologic agent of the periodontitis and is associated with extra-oral infections. In this study, the detection of the ltxA gene as well as the ltx promoter region from leukotoxic A. actinomycetemcomitans isolated from 50 Brazilian patients with periodontitis and 50 healthy subjects was performed. The leukotoxic activity on HL-60 cells was also evaluated. Leukotoxic activity was determined using a trypan blue exclusion method. The 530 bp deletion in the promoter region was evaluated by PCR using a PRO primer pair. A. actinomycetemcomitans was detected by culture and directly from crude subgingival biofilm by PCR using specific primers. By culture, A. actinomycetemcomitans was detected in nine (18%) of the periodontal patients and one (2%) healthy subject. However, by PCR, this organism was detected in 44% of the periodontal patients and in 16% of the healthy subjects. It was verified a great discrepancy between PCR detection of the ltx operon promoter directly from crude subgingival biofilm and from bacterial DNA. Only one periodontal sample harbored highly leukotoxic A. actinomycetemcomitans. Moreover, biotype II was the most prevalent and no correlation between biotypes and leukotoxic activity was observed. The diversity of leukotoxin expression by A. actinomycetemcomitans suggests a role of this toxin in the pathogenesis of periodontal disease and other infectious diseases.<br>Aggregatibacter actinomycetemcomitans é um importante agente etiológico da periodontite e produz infecções extra-bucais. Neste estudo, foram detectados os biótipos, o gene ltxA associado à produção de leucotoxina e o promotor ltx em A. actinomycetemcomitans de pacientes com e sem periodontite. A atividade leucotóxica sobre células HL-60 também foi avaliada. A atividade leucotóxica foi determinada através do método de exclusão do azul de tripam. A deleção de 530 bp no promotor ltx foi avaliada usando-se o par de iniciadores PRO. A. actinomycetemcomitans foi detectado por cultura e por PCR. Por cultura, A. actinomycetemcomitans foi detectado em nove pacientes com periodontite (18%) e em um indivíduo sadio (2%). Por PCR esse microrganismo foi detectado em 44% dos pacientes com periodontite e em 16% dos saudáveis. Verificou-se diferença estatística entre a detecção do promotor do operon ltx, por PCR, diretamente do biofilme subgengival e do DNA bacteriano. Somente uma amostra clínica apresentou A. actinomycetemcomitans altamente leukotóxico. O biótipo II foi o mais prevalente e não foi observada correlação biótipo-atividade leucotóxica. A expressão da leucotoxina por A. actinomycetemcomitans na doença periodontal e outras doenças infecciosas necessita ser avaliado

Surface topography and bacterial adhesion of CAD/ CAM resin based materials after application of different surface finishing techniques

is study evaluated the surface topography and bacterial adhesion of a hybrid ceramic and a nano ceramic resin composite after different surface finishes. Methods: Hybrid ceramic (Vita Enamic, VITA - EN) and nano ceramic resin composite (Lava Ultimate, 3M/ESPE - LV) blocks of 12 x 14 x 18 mm were cut into 1mm slices. Each slice was divided into four specimens (6 x 7 mm) that were randomly allocated into 4 groups (n=8) according to the surface finishing: CTL - without surface finish (control); DB - wear with a diamond bur; VT - polishing system for hybrid ceramic (VITA); and DD - polishing system for ceramics (Dedeco). The specimens were analyzed regarding surface roughness parameters (Ra, Rz, Rq), sterilized and subjected to bacterial adhesion. Representative specimens from each group were observed by SEM and Confocal Laser Scanning Microscopy. Data were submitted to two-way ANOVA and Tukeys test (&#945;=0.05). Results: EN had lower surface roughness and bacterial adhesion than LV (p0.05). The type of material and surface finish system significantly interfered with surface roughness parameters and bacterial adhesion. The hybrid ceramic performed better after polishing than the nano-ceramic resin. Conclusion: An adequate finishing/polishing technique should always be performed after any kind of adjustment to indirect restorations made with these materials tested