The use of germinants to potentiate the sensitivity of Bacillus anthracis spores to peracetic acid

Elimination of Bacillus anthracis spores from the environment is a difficult and costly process due in part to the toxicity of current sporicidal agents. For this reason we investigated the ability of the spore germinants L-alanine (100 mM) and inosine (5 mM) to reduce the concentration of peracetic acid (PAA) required to inactivate B. anthracis spores. While L-alanine significantly enhanced (p = 0.0085) the bactericidal activity of 500 ppm PAA the same was not true for inosine suggesting some form of negative interaction. In contrast the germinant combination proved most effective at 100 ppm PAA (p = 0.0009). To determine if we could achieve similar results in soil we treated soil collected from the burial site of an anthrax infected animal which had been supplemented with spores of the Sterne strain of B. anthracis to increase the level of contamination to 104 spores/g. Treatment with germinants followed 1 h later by 5000 ppm PAA eliminated all of the spores. In contrast direct treatment of the animal burial site using this approach delivered using a back pack sprayer had no detectable effect on the level of B. anthracis contamination or on total culturable bacterial numbers over the course of the experiment. It did trigger a significant, but temporary, reduction (p < 0.0001) in the total spore count suggesting that germination had been triggered under real world conditions. In conclusion, we have shown that the application of germinants increase the sensitivity of bacterial spores to PAA. While the results of the single field trial were inconclusive, the study highlighted the potential of this approach and the challenges faced when attempting to perform real world studies on B. anthracis spores contaminated sites

Supporting Real-time Peer-Mentoring of Rural Volunteers

Telephone-driven community forums have been a widely proposed solution to address the unreliable internet connectivity and large geographic scope that characterizes many international NGO contexts. Primarily, these applications support asynchronous activities, such as information portals or forums to access rural journalism, but opportunities for real-time experience sharing remain largely under-explored. In this paper, we explore the potential of such forums to support remote mentoring of NGO volunteers, a practice that requires synchronous, dialogical formats for experience sharing and peer discussion. We engaged 28 participants from a rural Indian NGO in the design of peer-mentoring sessions that leverage synchronous audio discussions, using the structure and format of traditional talk-show radio as a starting point. The participants favored an entertaining approach to mentoring and discussed the logistics required to achieve this within their resource constraints. We conclude with design implications for designing media-driven community engagement platforms and the ethical challenges around protecting marginalized community interests

An expression atlas of human primary cells: inference of gene function from coexpression networks

BACKGROUND: The specialisation of mammalian cells in time and space requires genes associated with specific pathways and functions to be co-ordinately expressed. Here we have combined a large number of publically available microarray datasets derived from human primary cells and analysed large correlation graphs of these data. RESULTS: Using the network analysis tool BioLayout Express(3D) we identify robust co-associations of genes expressed in a wide variety of cell lineages. We discuss the biological significance of a number of these associations, in particular the coexpression of key transcription factors with the genes that they are likely to control. CONCLUSIONS: We consider the regulation of genes in human primary cells and specifically in the human mononuclear phagocyte system. Of particular note is the fact that these data do not support the identity of putative markers of antigen-presenting dendritic cells, nor classification of M1 and M2 activation states, a current subject of debate within immunological field. We have provided this data resource on the BioGPS web site (http://biogps.org/dataset/2429/primary-cell-atlas/) and on macrophages.com (http://www.macrophages.com/hu-cell-atlas)

Sustainability of beef production from brigalow lands after cultivation and mining. 1. Sown pasture growth and carrying capacity

Context: New Acland coal mine in south-eastern Queensland is seeking to rehabilitate mined land to pastures that are safe, stable and sustainable for beef production. Little is known of the productivity and sustainability of grazing previously mined land in the Darling Downs study region. Additionally, information is required to specify management guidelines for sustainable grazing of regional land types retired from cultivation. Aims: Identify pasture growth characteristics, rainfall use efficiencies and long-term carrying capacities of subtropical sown pastures established on lands rehabilitated after open-cut coal mining in comparison to sown pastures established on un-mined but previously cultivated lands. Methods: Pasture growth and quality (% nitrogen) were observed using the Swiftsynd methodology in ungrazed exclosures with three sites on rehabilitated lands of the Acland Grazing Trial over a 5-year period (2014–2018), and 13 sites on unmined lands over periods of 2–5 years providing data for modelling pasture growth. Key results: Peak pasture yield (TSDM for autumn harvests) averaged for 2017 and 2018 was greater (P < 0.1) on rehabilitated sites than unmined Poplar Box land type sites (5957 and 2233 kg/ha respectively) but similar to Brigalow Uplands and Mountain Coolibah land type sites (3946 and 3413 kg/ha respectively). Pasture rundown was evident, with pasture N uptake decreasing over 5 years at some sites. Soil mineral N supply (potentially mineralisable N and mineral N) in spring was a useful indicator of N uptake over the following growing season. Simulations using the GRASP pasture growth model for the grazing trial period predicted rainfall use efficiencies of 12.0, 7.0, 9.1 and 4.8 kg/ha.mm rainfall for rehabilitated sites and unmined sites on Brigalow Uplands, Mountain Coolibah and Poplar Box land types respectively. Long-term carrying capacities based on estimates of long-term median pasture growth and 30% utilisation were 4.39, 3.58 and 5.92 ha/adult equivalent respectively for the unmined land types, and 2.45 ha/adult equivalent for the rehabilitated lands. Conclusions: Rehabilitated land can be as productive as unmined but previously cultivated land. Implications: Grazing management plans for sustainable management of mined and unmined lands can be developed using data from the present study. The plans will assist with the transition of rehabilitated lands to commercial agriculture

Sustainability of beef production from brigalow lands after cultivation and mining. 3. Pasture rundown, climate and grazing pressure effects

Context: The Acland Land System overlying the Walloon sandstone coal deposits in southern Queensland is generally marginal for cropping but well suited to grazing, and thus cultivated land is commonly returned to pasture. Rehabilitation of these lands after open-cut coal mining seeks to be safe, stable and self-sustaining to satisfy requirements for ecologically sustainable development. Aims: The present paper evaluates the sustainability and economic viability of beef production on (a) lands retired from cultivation and then rehabilitated with sown pastures after open-cut coal mining at the New Acland mine site, and (b) similar nearby pasture lands that were not mined but were also retired from cultivation. Methods: The GRASP grazing systems model was modified and calibrated with short-term (5-year) grazing trial data (soil, pasture and cattle observations), and then used with long-term (60-year) weather data to estimate effects of land type, pasture rundown, climate and grazing pressure on productivity and economic returns. The productivity of three rehabilitated sites and 15 unmined sites were evaluated, including pastures on six commercial properties. Key results: Estimates of long-term mean annual growth of pastures on unmined lands retired from cultivation on three land types (Mountain Coolibah, Brigalow Uplands and Poplar Box) were 3398, 2817 and 2325 kg/ha respectively. Pasture growth was greater on rehabilitated lands; 3736 kg/ha on the site most typical of rehabilitated lands and a mean of 4959 kg/ha across three sites. Seasonal conditions had large effects on cattle liveweight gain (133–213 kg/head per year during the trial); however, pasture growth was the main driver of beef production and economic returns per hectare. In GRASP, potential nitrogen uptake was used to influence key pasture growth processes and accounted for 64% of variation in observed annual growth. The short-term lift and subsequent rundown in productivity typically associated with sown pastures was estimated to have increased mean annual pasture and cattle productivity during the 2014–2018 trial period by up to 17% and 25% respectively. Estimates of long-term mean annual beef production and economic returns for the unmined lands were less than estimated for rehabilitated lands and were 139 kg/head.year (45 kg/ha.year) and AU$154/adult equivalent. Conclusions: Rehabilitated lands were found to be sustainable for beef production at grazing pressures up to 30% utilisation of annual pasture growth, and comparable with grazing systems on native and sown pastures in good condition. Pastures on unmined lands retired from cultivation had reduced productivity. Implications: Overgrazing is a significant and on-going residual risk to sustainable production. Grazing regimes need to continually adjust for changes in novel landscapes, pasture condition and climate. The methods used in the present study could be applied more generally

Admission Blood Glucose Level and Its Association With Cardiovascular and Renal Complications in Patients Hospitalized With COVID-19

OBJECTIVE: To investigate the association between admission blood glucose levels and risk of in-hospital cardiovascular and renal complications. RESEARCH DESIGN AND METHODS: In this multicenter prospective study of 36,269 adults hospitalized with COVID-19 between 6 February 2020 and 16 March 2021 (N = 143,266), logistic regression models were used to explore associations between admission glucose level (mmol/L and mg/dL) and odds of in-hospital complications, including heart failure, arrhythmia, cardiac ischemia, cardiac arrest, coagulation complications, stroke, and renal injury. Nonlinearity was investigated using restricted cubic splines. Interaction models explored whether associations between glucose levels and complications were modified by clinically relevant factors. RESULTS: Cardiovascular and renal complications occurred in 10,421 (28.7%) patients; median admission glucose level was 6.7 mmol/L (interquartile range 5.8-8.7) (120.6 mg/dL [104.4-156.6]). While accounting for confounders, for all complications except cardiac ischemia and stroke, there was a nonlinear association between glucose and cardiovascular and renal complications. For example, odds of heart failure, arrhythmia, coagulation complications, and renal injury decreased to a nadir at 6.4 mmol/L (115 mg/dL), 4.9 mmol/L (88.2 mg/dL), 4.7 mmol/L (84.6 mg/dL), and 5.8 mmol/L (104.4 mg/dL), respectively, and increased thereafter until 26.0 mmol/L (468 mg/dL), 50.0 mmol/L (900 mg/dL), 8.5 mmol/L (153 mg/dL), and 32.4 mmol/L (583.2 mg/dL). Compared with 5 mmol/L (90 mg/dL), odds ratios at these glucose levels were 1.28 (95% CI 0.96, 1.69) for heart failure, 2.23 (1.03, 4.81) for arrhythmia, 1.59 (1.36, 1.86) for coagulation complications, and 2.42 (2.01, 2.92) for renal injury. For most complications, a modifying effect of age was observed, with higher odds of complications at higher glucose levels for patients age <69 years. Preexisting diabetes status had a similar modifying effect on odds of complications, but evidence was strongest for renal injury, cardiac ischemia, and any cardiovascular/renal complication. CONCLUSIONS: Increased odds of cardiovascular or renal complications were observed for admission glucose levels indicative of both hypo- and hyperglycemia. Admission glucose could be used as a marker for risk stratification of high-risk patients. Further research should evaluate interventions to optimize admission glucose on improving COVID-19 outcomes

Admission Blood Glucose Level and Its Association With Cardiovascular and Renal Complications in Patients Hospitalized With COVID-19

ObjectiveTo investigate the association between admission blood glucose levels and risk of in-hospital cardiovascular and renal complications.Research design and methodsIn this multicenter prospective study of 36,269 adults hospitalized with COVID-19 between 6 February 2020 and 16 March 2021 (N = 143,266), logistic regression models were used to explore associations between admission glucose level (mmol/L and mg/dL) and odds of in-hospital complications, including heart failure, arrhythmia, cardiac ischemia, cardiac arrest, coagulation complications, stroke, and renal injury. Nonlinearity was investigated using restricted cubic splines. Interaction models explored whether associations between glucose levels and complications were modified by clinically relevant factors.ResultsCardiovascular and renal complications occurred in 10,421 (28.7%) patients; median admission glucose level was 6.7 mmol/L (interquartile range 5.8-8.7) (120.6 mg/dL [104.4-156.6]). While accounting for confounders, for all complications except cardiac ischemia and stroke, there was a nonlinear association between glucose and cardiovascular and renal complications. For example, odds of heart failure, arrhythmia, coagulation complications, and renal injury decreased to a nadir at 6.4 mmol/L (115 mg/dL), 4.9 mmol/L (88.2 mg/dL), 4.7 mmol/L (84.6 mg/dL), and 5.8 mmol/L (104.4 mg/dL), respectively, and increased thereafter until 26.0 mmol/L (468 mg/dL), 50.0 mmol/L (900 mg/dL), 8.5 mmol/L (153 mg/dL), and 32.4 mmol/L (583.2 mg/dL). Compared with 5 mmol/L (90 mg/dL), odds ratios at these glucose levels were 1.28 (95% CI 0.96, 1.69) for heart failure, 2.23 (1.03, 4.81) for arrhythmia, 1.59 (1.36, 1.86) for coagulation complications, and 2.42 (2.01, 2.92) for renal injury. For most complications, a modifying effect of age was observed, with higher odds of complications at higher glucose levels for patients age ConclusionsIncreased odds of cardiovascular or renal complications were observed for admission glucose levels indicative of both hypo- and hyperglycemia. Admission glucose could be used as a marker for risk stratification of high-risk patients. Further research should evaluate interventions to optimize admission glucose on improving COVID-19 outcomes

Smart homes and their users:a systematic analysis and key challenges

Published research on smart homes and their users is growing exponentially, yet a clear understanding of who these users are and how they might use smart home technologies is missing from a field being overwhelmingly pushed by technology developers. Through a systematic analysis of peer-reviewed literature on smart homes and their users, this paper takes stock of the dominant research themes and the linkages and disconnects between them. Key findings within each of nine themes are analysed, grouped into three: (1) views of the smart home-functional, instrumental, socio-technical; (2) users and the use of the smart home-prospective users, interactions and decisions, using technologies in the home; and (3) challenges for realising the smart home-hardware and software, design, domestication. These themes are integrated into an organising framework for future research that identifies the presence or absence of cross-cutting relationships between different understandings of smart homes and their users. The usefulness of the organising framework is illustrated in relation to two major concerns-privacy and control-that have been narrowly interpreted to date, precluding deeper insights and potential solutions. Future research on smart homes and their users can benefit by exploring and developing cross-cutting relationships between the research themes identified