Effects of infestation by cowpea aphid (Homoptera: Aphididae) on different growth stages of resistant and susceptible cowpea cultivars

Field studies were conducted to characterise the effects of infestations by adult and nymphal stages of cowpea aphid, Aphis craccivora Koch, on the growth and yield of cowpea, Vigna unguiculata (L.) Walp. Seedling, flowering, and podding stage plants of aphid-resistant (cv. ICV-12) and aphid-susceptible (cv. ICV-1) cowpea cultivars were used in the studies. Four treatments (consisting of infestations with adult and nymphal aphids, caged controls and uncaged controls) were administered on plants for 22 days post-treatment. Eight parameters of crop success were measured: extended leaf heights (ELH); plant mortality; incidence of sooty mould; incidence and abundance of natural enemy species; crop growth parameters (net assimilation rate, [NAR] in g/ dm2/day, and crop growth rate [CGR] in g/dm2 land surface/day); and plant yields (seeds per pod, weight per seed). Data were analysed using analysis of variance (ANOVA), orthogonal contrasts and 95% confidence intervals (C.I.). There were no significant (P > 0.05) differences between adult and nymphal infestations or between caged and uncaged controls, so the respective sets of data were combined for comparisons of aphid infestations with control treatments. Infestations caused severe plant stunting and other growth deformities, drastic yield reductions, higher plant mortality, greater incidence of natural enemies and abundance of Cheilomenes spp. on cv. ICV-1 than on cv. ICV-12, and on infested and uninfested plants. Aphid infestations did not significantly affect the incidence of sooty mould on plants of cv. ICV-12 or cv. ICV-

Pattern and rate of within-field dispersal and bionomics of the cowpea aphid, Aphis craccivora (Aphididae), on selected cowpea cultivars

Comparative field studies were conducted during the rainy and dry seasons at the Mbita Point Field Station of ICIPE in southwestern Kenya, to investigate the pattern and rate of dispersal of Aphis craccivora on aphid-resistant (ICV-12) and aphid-susceptible (ICV-1) cowpea cultivars in relation to key weather factors. The effects of the dispersal trends on crop performance and aphid population dynamics were analysed. Treatments consisted of initial aphid releases at the north, south, west, east and centre of test plots and uninfested controls maintained on plants for 22 days. Parameters recorded included: pattern (direction of spread of aphids) and rate (number of aphid-infested plants at a given time) in test plots; crop growth and yields and associated factors such as incidence of sooty mould and plant mortality, and aphid density and associated factors, including the incidence of natural enemy species, particularly coccinellids. Dispersal was fastest when releases were made in the west, north and centre of plots, and resulted in adveVse effects on ICV-1 growth and yields. Infestations of ICV-12 did not significantly affect crop performance. There was an apparent direct, positive relationship between wind direction and pattern of spread of aphid infestations, but this pattern was more apparent during the rainy season, when wind speeds were higher, than during the dry season. The incidence of natural enemy species correlated with the spread of aphid infestations, while the abundance of coccinellids correlated with aphid densit

Stylet Penetration Activities by Aphis craccivora (Homoptera: Aphididae) on Plants and Excised Plant Parts of Resistant and Susceptible Cultivars of Cowpea (Leguminosae)

Direct current electrical penetration graphs (DC-EPGs) were used to analyze the stylet penetration activities of cowpea aphid, Aphis craccivora Koch, on plants of aphid-resistant (ICV-12) and aphid-susceptible (ICV-1) cultivars of cowpea, Vigna unguiculata (L.) Walpers. Aphid stylet penetration on whole plants at seedling, flowering, and podding stages were studied in one experiment, and in another experiment excised leaves from seedling plants, excised flowers, and excised pods were tested. Electrical signals depicting the aphid stylet penetration activities on their host plants were amplified, recorded onto a paper chart recorder, and scored for specific waveform patterns. Compared with similar tissues of ICV-1, intact leaves and excised seedling foliage of ICV-12 plants caused severe disruption of aphid stylet penetration activities. This was manifested in frequent penetration attempts that were abruptly terminated or unsustained, and in shorter penetration times, signifying antixenosis resistance in ICV-12. There was reduced occurrence of E waveforms, which represent stylet activity in plant vascular tissues. Also, prior exposure of test aphids to plants of one cultivar did not significantly influence the expected stylet penetration activities on plants of the other cultivar. Overall, ICV-12 exhibited high levels of resistance against A. craccivor

Performance of a North American Field Population and a Laboratory Colony of the Potato Tuberworm, Phthorimaea operculella, on Foliage of Resistant and Susceptible Potato Clones

Foliar resistance of two potato clones was tested against a Columbia Basin field population (CBFP) and a Colorado laboratory colony (COLC) of the potato tuberworm, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae). The first clone was a cross of a cultivated potato, Solanum tuberosum L. (Solanales: Solanaceae), and a wild potato, Solanum berthaultii Hawkes (Q 174-2); the second clone was cv. Allegany, S. tuberosum L.. In no-choice assays, defoliation by P. operculella larvae of COLC and CBFP did not differ on Allegany and Q174-2. Larval weight and production of COLC and CBFP colonies were similarly reduced on Q174-2 compared to cv. Allegany, although larval weights and production of the CBFP population were slightly less affected by the host. Larval production by the COLC on Allegany was greater than that on Q174-2, while that of the CBFP on Allegany and Q174-2 did not differ. However, production of P. operculella larvae by the CBFP on Q174-2 during no-choice assays was greater than that in choice tests, indicating reduced host preference. Most of the larvae recovered from either host were fourth instars, followed by third instars. Although the levels of resistance expressed by Q174-2 potato clone to the two P. operculella populations differed in magnitude, nearly all of P. operculella performance criteria measured in this study were adversely affected by Q174-2 foliage compared to the commercial potato cultivar, cv. Allegany

Large subglacial source of mercury from the southwestern margin of the Greenland Ice Sheet

The Greenland Ice Sheet is currently not accounted for in Arctic mercury budgets, despite large and increasing annual runoff to the ocean and the socio-economic concerns of high mercury levels in Arctic organisms. Here we present concentrations of mercury in meltwaters from three glacial catchments on the southwestern margin of the Greenland Ice Sheet and evaluate the export of mercury to downstream fjords based on samples collected during summer ablation seasons. We show that concentrations of dissolved mercury are among the highest recorded in natural waters and mercury yields from these glacial catchments (521–3,300 mmol km−2 year−1) are two orders of magnitude higher than from Arctic rivers (4–20 mmol km−2 year−1). Fluxes of dissolved mercury from the southwestern region of Greenland are estimated to be globally significant (15.4–212 kmol year−1), accounting for about 10% of the estimated global riverine flux, and include export of bioaccumulating methylmercury (0.31–1.97 kmol year−1). High dissolved mercury concentrations (~20 pM inorganic mercury and ~2 pM methylmercury) were found to persist across salinity gradients of fjords. Mean particulate mercury concentrations were among the highest recorded in the literature (~51,000 pM), and dissolved mercury concentrations in runoff exceed reported surface snow and ice values. These results suggest a geological source of mercury at the ice sheet bed. The high concentrations of mercury and its large export to the downstream fjords have important implications for Arctic ecosystems, highlighting an urgent need to better understand mercury dynamics in ice sheet runoff under global warming